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Searching for up to 100 curated homologs for SM_b20577 FitnessBrowser__Smeli:SM_b20577 (253 a.a.)

Found high-coverage hits (≥70%) to 38 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

A0A193DXP2 protocatechuate 3,4-dioxygenase (subunit 1/2) (EC 1.13.11.3) from Rhizobium sp. LMB-1 (see paper)
    78% identity, 96% coverage of query (398 bits)

pcaH / AAF34268.1 protocatechuate 3,4-dioxygenase beta chain from Agrobacterium tumefaciens (see 2 papers)
    77% identity, 96% coverage of query (395 bits)

pcaH / P00437 protocatechuate 3,4-dioxygenase β subunit (EC 1.13.11.3) from Pseudomonas putida (see paper)
pcaH / AAB41024.1 protocatechuate 3,4-dioxygenase, beta subunit from Pseudomonas putida (see paper)
    61% identity, 93% coverage of query (295 bits)

P00437 Protocatechuate 3,4-dioxygenase beta chain; 3,4-PCD; EC 1.13.11.3 from Pseudomonas putida (Arthrobacter siderocapsulatus)
    61% identity, 93% coverage of query (295 bits)

4whrB Anhydride reaction intermediate trapped in protocatechuate 3,4- dioxygenase (pseudomonas putida) at ph 8.5
    61% identity, 93% coverage of query (295 bits)

4whqB Alkylperoxo reaction intermediate trapped in protocatechuate 3,4- dioxygenase (pseudomonas putida) at ph 6.5
    61% identity, 93% coverage of query (295 bits)

I0DHJ1 protocatechuate 3,4-dioxygenase (subunit 1/2) (EC 1.13.11.3) from Stenotrophomonas maltophilia (see 2 papers)
    59% identity, 98% coverage of query (294 bits)

3lktM / P00437 Tyrosine 447 of protocatechuate 3,4-dioxygenase controls efficient progress through catalysis
    60% identity, 93% coverage of query (293 bits)

3pckM Structure of protocatechuate 3,4-dioxygenase complexed with 6- hydroxynicotinic acid n-oxide
    61% identity, 92% coverage of query (293 bits)

3pcjM Structure of protocatechuate 3,4-dioxygenase complexed with 2- hydroxyisonicotinic acid n-oxide
    61% identity, 92% coverage of query (293 bits)

3pciM Structure of protocatechuate 3,4-dioxygenase complexed with 3-iodo-4- hydroxybenzoate
    61% identity, 92% coverage of query (293 bits)

3pchM Structure of protocatechuate 3,4-dioxygenase complexed with 3-chloro- 4-hydroxybenzoate
    61% identity, 92% coverage of query (293 bits)

3pcgM Structure of protocatechuate 3,4-dioxygenase complexed with the inhibitor 4-hydroxyphenylacetate
    61% identity, 92% coverage of query (293 bits)

3pcfM Structure of protocatechuate 3,4-dioxygenase complexed with 3-fluro-4- hydroxybenzoate
    61% identity, 92% coverage of query (293 bits)

3pceM Structure of protocatechuate 3,4-dioxygenase complexed with 3- hydroxyphenylacetate
    61% identity, 92% coverage of query (293 bits)

3pcbM Structure of protocatechuate 3,4-dioxygenase complexed with 3- hydroxybenzoate
    61% identity, 92% coverage of query (293 bits)

3lxvM Tyrosine 447 of protocatechuate 3,4-dioxygenase controls efficient progress through catalysis
    60% identity, 93% coverage of query (293 bits)

4whqF Alkylperoxo reaction intermediate trapped in protocatechuate 3,4- dioxygenase (pseudomonas putida) at ph 6.5
    61% identity, 92% coverage of query (293 bits)

3mv4M Axial ligand swapping in double mutant maintains intradiol-cleavage chemistry in protocatechuate 3,4-dioxygenase
    60% identity, 93% coverage of query (291 bits)

3mi5M Axial ligand swapping in double mutant maintains intradiol-cleavage chemistry in protocatechuate 3,4-dioxygenase
    60% identity, 93% coverage of query (291 bits)

3mflM Axial ligand swapping in double mutant maintains intradiol-cleavage chemistry in protocatechuate 3,4-dioxygenase
    60% identity, 93% coverage of query (291 bits)

A8I4B3 protocatechuate 3,4-dioxygenase (EC 1.13.11.3) from Chromohalobacter sp. (see paper)
    56% identity, 98% coverage of query (287 bits)

pcaH / RF|YP_046375.1 protocatechuate 3,4-dioxygenase, beta chain; EC 1.13.11.3 from Acinetobacter sp. ADP1 (see 3 papers)
pcaH / AAC37153.1 protocatechuate 3,4-dioxygenase beta subunit from Acinetobacter baylyi (see 11 papers)
    58% identity, 92% coverage of query (284 bits)

2bumB / P20372 Crystal structure of wild-type protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 (see paper)
    58% identity, 92% coverage of query (283 bits)

2buqB Crystal structure of wild-type protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 in complex with catechol
    58% identity, 92% coverage of query (283 bits)

1eocB Crystal structure of acinetobacter sp. Adp1 protocatechuate 3,4- dioxygenase in complex with 4-nitrocatechol
    58% identity, 92% coverage of query (283 bits)

1eoaB Crystal structure of acinetobacter sp. Adp1 protocatechuate 3,4- dioxygenase in complex with cyanide
    58% identity, 92% coverage of query (283 bits)

1eo9B Crystal structure of acinetobacter sp. Adp1 protocatechuate 3,4- dioxygenase at ph < 7.0
    58% identity, 92% coverage of query (283 bits)

Q0SH27 protocatechuate 3,4-dioxygenase (subunit 2/2) (EC 1.13.11.3) from Rhodococcus jostii (see paper)
    53% identity, 89% coverage of query (233 bits)

pcaH / AAC38243.1 protocatechuate dioxygenase beta subunit from Rhodococcus opacus (see paper)
    52% identity, 89% coverage of query (232 bits)

pcaH2 / Q93PS2 protocatechuate 3,4-dioxygenase type II β subunit (EC 1.13.11.3) from Hydrogenophaga intermedia (see 4 papers)
    48% identity, 88% coverage of query (209 bits)

2buqA / P20371 Crystal structure of wild-type protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 in complex with catechol (see paper)
    32% identity, 74% coverage of query (83.6 bits)

pcaG / RF|YP_046376.1 protocatechuate 3,4-dioxygenase, alpha chain; EC 1.13.11.3 from Acinetobacter sp. ADP1 (see 3 papers)
    32% identity, 74% coverage of query (83.6 bits)

2buuA Crystal structure of protocatechuate 3,4-dioxygenase from acinetobacter sp. Adp1 mutant r457s in complex with 4-nitrocatechol
    32% identity, 74% coverage of query (83.6 bits)

pcaG / AAC37154.1 protocatechuate 3,4-dioxygenase alpha subunit from Acinetobacter baylyi (see 11 papers)
    32% identity, 74% coverage of query (81.6 bits)

A8I4B7 protocatechuate 3,4-dioxygenase (EC 1.13.11.3) from Chromohalobacter sp. (see paper)
    32% identity, 80% coverage of query (77.8 bits)

5td3A Crystal structure of catechol 1,2-dioxygenase from burkholderia vietnamiensis
    34% identity, 78% coverage of query (76.6 bits)

Build an alignment

Build an alignment for SM_b20577 and 37 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

Cgl1160 / Q8NR92 hydroxyquinol 1,2-dioxygenase (EC 1.13.11.37) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see 2 papers)
HXQD1_CORGL / Q8NR92 Hydroxyquinol 1,2-dioxygenase; EC 1.13.11.37 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
    28% identity, 75% coverage of query (53.9 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory