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Searching for up to 100 curated homologs for SM_b20579 FitnessBrowser__Smeli:SM_b20579 (268 a.a.)

Found high-coverage hits (≥70%) to 80 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

pcaD / AAF34270.1 beta-ketoadipate enol-lactone hydrolase from Agrobacterium tumefaciens (see 2 papers)
    63% identity, 98% coverage of query (342 bits)

pcaL / AAC38246.1 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Rhodococcus opacus (see paper)
    38% identity, 89% coverage of query (162 bits)

2xuaH / Q13KT2 Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
    36% identity, 97% coverage of query (160 bits)

Q13KT2 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Paraburkholderia xenovorans (see 2 papers)
    36% identity, 97% coverage of query (160 bits)

Q0SH24 3-oxoadipate enol-lactonase (EC 3.1.1.24); 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) from Rhodococcus jostii (see paper)
    37% identity, 89% coverage of query (155 bits)

catD / BAA75208.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see paper)
catD / BAC82535.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see 3 papers)
    39% identity, 93% coverage of query (154 bits)

catD / AAC46435.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 8 papers)
    34% identity, 94% coverage of query (153 bits)

pcaD / Q88N36 subunit of 3-oxoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 5 papers)
Q88N36 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Pseudomonas putida (see paper)
    38% identity, 94% coverage of query (152 bits)

AO353_17230 Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas fluorescens FW300-N2E3
    35% identity, 93% coverage of query (145 bits)

pcaD / AAC37150.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 11 papers)
    34% identity, 90% coverage of query (135 bits)

bioH / BAB39459.1 BioH from Kurthia sp. 538-KA26 (see paper)
    30% identity, 92% coverage of query (100 bits)

Build an alignment

Build an alignment for SM_b20579 and 11 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

6eb3B Structural and enzymatic characterization of an esterase from a metagenomic library
    28% identity, 97% coverage of query (90.9 bits)

6eb3A Structural and enzymatic characterization of an esterase from a metagenomic library
    28% identity, 97% coverage of query (89.7 bits)

6eb3C Structural and enzymatic characterization of an esterase from a metagenomic library
    29% identity, 97% coverage of query (89.0 bits)

4uhfA / A0A0M3KKY6 Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
    26% identity, 89% coverage of query (85.9 bits)

4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound)
    26% identity, 89% coverage of query (85.9 bits)

4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound)
    26% identity, 89% coverage of query (85.9 bits)

BPOC_MYCTU / P9WNH1 Putative non-heme bromoperoxidase BpoC from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
    28% identity, 89% coverage of query (77.8 bits)

PFMAB_PESFW / W3XA95 Proline iminopeptidase PfmaB; PIP; Conidial pigment biosynthesis cluster protein B; EC 3.4.11.5 from Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) (see 2 papers)
    25% identity, 96% coverage of query (77.0 bits)

aamsh1 / Q988D4 2-(acetamidomethylene)succinate hydrolase monomer (EC 3.5.1.29) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (see 7 papers)
AAMHY_RHILO / Q988D4 2-(acetamidomethylene)succinate hydrolase; alpha-(N-acetylaminomethylene)succinic acid amidohydrolase; AAMS amidohydrolase; EC 3.5.1.29 from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) (see 2 papers)
Q988D4 2-(acetamidomethylene)succinate hydrolase (EC 3.5.1.29) from Mesorhizobium loti (see 2 papers)
    28% identity, 92% coverage of query (73.2 bits)

5frdA / O28735 Structure of a thermophilic esterase (see paper)
    28% identity, 88% coverage of query (72.8 bits)

EstN1 / K0IAM1 pimeloyl-[acyl-carrier protein] methyl ester esterase (EC 3.1.1.85) from Nitrososphaera gargensis (strain Ga9.2) (see paper)
    27% identity, 88% coverage of query (69.3 bits)

O73957 carboxylesterase (EC 3.1.1.1) from Sulfolobus acidocaldarius (see paper)
    25% identity, 95% coverage of query (68.6 bits)

5z7xA / A0A0M4AMQ0 Crystal structure of striga hermonthica htl4 (shhtl4) (see paper)
    24% identity, 92% coverage of query (63.9 bits)

flnE / BAC75995.1 meta cleavage compound hydrolase from Terrabacter sp. DBF63 (see paper)
    25% identity, 90% coverage of query (62.8 bits)

C7TMK0 prolyl aminopeptidase (EC 3.4.11.5) from Lacticaseibacillus rhamnosus (see paper)
    24% identity, 96% coverage of query (60.8 bits)

5h3hB / K0ACL0 Esterase (eaest) from exiguobacterium antarcticum (see paper)
    25% identity, 77% coverage of query (60.5 bits)

5cbkA / A0A0M5I297 Crystal structure of the strigolactone receptor shhtl5 from striga hermonthica (see paper)
    22% identity, 88% coverage of query (59.7 bits)

Q8KT44 carboxylesterase (EC 3.1.1.1) from Lacticaseibacillus casei (see paper)
    25% identity, 81% coverage of query (59.3 bits)

A0A286S6I1 quorum-quenching N-acyl-homoserine lactonase (EC 3.1.1.81) from uncultured bacterium (see paper)
    26% identity, 96% coverage of query (58.9 bits)

8dvcA Receptor shhtl5 from striga hermonthica in complex with strigolactone agonist gr24
    22% identity, 88% coverage of query (58.9 bits)

C2F9L5 prolyl aminopeptidase (EC 3.4.11.5) from Lacticaseibacillus paracasei subsp. paracasei (see paper)
    23% identity, 96% coverage of query (58.5 bits)

D14L_ORYSJ / Q10J20 Probable esterase D14L; Protein DWARF-14-like; Protein D14-like; Protein HEBIBA D14L from Oryza sativa subsp. japonica (Rice) (see paper)
    25% identity, 92% coverage of query (58.2 bits)

BIOH_NEIMH / E6MWF8 Pimeloyl-[acyl-carrier protein] methyl ester esterase; Biotin synthesis protein BioH; Carboxylesterase BioH; EC 3.1.1.85 from Neisseria meningitidis serogroup B / serotype 15 (strain H44/76) (see paper)
    28% identity, 87% coverage of query (57.8 bits)

1xrmA Crystal structure of active site f1-mutant e213q soaked with peptide ala-phe
    22% identity, 96% coverage of query (57.4 bits)

1mu0A / P96084 Crystal structure of the tricorn interacting factor f1 complex with pck (see paper)
pip / GB|AAC44636.1 prolyl aminopeptidase; EC 3.4.11.5 from Thermoplasma acidophilum (see 3 papers)
    22% identity, 96% coverage of query (57.0 bits)

KAI2_ARATH / Q9SZU7 Probable esterase KAI2; Protein DWARF-14-like; Protein D14-like; Protein HYPOSENSITIVE TO LIGHT; Protein KARRIKIN INSENSITIVE 2 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
    23% identity, 93% coverage of query (56.6 bits)

4jymA / Q9SZU7 Crystal structure of kai2 in complex with 3-methyl-2h-furo[2,3- c]pyran-2-one (see paper)
    23% identity, 93% coverage of query (56.2 bits)

8agsAAA / A0A1U9WZ52 8agsAAA
    23% identity, 93% coverage of query (55.8 bits)

6brtA / A0A0D9Z3K8 F-box protein cth with hydrolase (see paper)
    25% identity, 92% coverage of query (55.8 bits)

D14_ORYSJ / Q10QA5 Strigolactone esterase D14; Protein DWARF 14; Protein DWARF 88; Protein HIGH-TILLERING DWARF 2; EC 3.1.-.- from Oryza sativa subsp. japonica (Rice) (see 11 papers)
    25% identity, 92% coverage of query (55.8 bits)

5zhsA Crystal structure of osd14 in complex with covalently bound kk052
    25% identity, 92% coverage of query (55.8 bits)

8agnAAA Alpha/beta epoxide hydrolase
    23% identity, 93% coverage of query (55.8 bits)

8agmAAA Alpha/beta epoxide hydrolase
    23% identity, 93% coverage of query (55.8 bits)

4ihaA Crystal structure of rice dwarf14 (d14) in complex with a gr24 hydrolysis intermediate
    25% identity, 92% coverage of query (55.8 bits)

5zhtA Crystal structure of osd14 in complex with covalently bound kk073
    25% identity, 92% coverage of query (55.8 bits)

5zhrA Crystal structure of osd14 in complex with covalently bound kk094
    25% identity, 92% coverage of query (55.8 bits)

5yz7A Crystal structure of osd14 in complex with d-ring-opened 7'-carba-4bd
    25% identity, 92% coverage of query (55.8 bits)

5ng7B Novel epoxide hydrolases belonging to the alpha/beta hydrolases superfamily in metagenomes from hot environments
    23% identity, 93% coverage of query (55.8 bits)

8agpAAA Alpha/beta epoxide hydrolase
    23% identity, 93% coverage of query (55.8 bits)

6ap8A Crystal structure of rice d14 bound to 2-(2-methyl-3-nitroanilino) benzoic acid
    25% identity, 92% coverage of query (55.8 bits)

5dj5A Crystal structure of rice dwarf14 in complex with synthetic strigolactone gr24
    25% identity, 92% coverage of query (55.8 bits)

1xqyA Crystal structure of f1-mutant s105a complex with pro-leu-gly-gly
    22% identity, 96% coverage of query (55.8 bits)

1xqxA Crystal structure of f1-mutant s105a complex with pck
    22% identity, 96% coverage of query (55.8 bits)

1xqwA Crystal structure of f1-mutant s105a complex with phe-leu
    22% identity, 96% coverage of query (55.8 bits)

7snuA / A0A0M3PNA2 Crystal structure of shhtl7 from striga hermonthica in complex with strigolactone antagonist rg6 (see paper)
    24% identity, 85% coverage of query (55.5 bits)

5z7wB / A0A0M4AV81 Crystal structure of striga hermonthica htl1 (shhtl1) (see paper)
    23% identity, 94% coverage of query (55.5 bits)

5z7yA Crystal structure of striga hermonthica htl7 (shhtl7)
    24% identity, 85% coverage of query (55.5 bits)

7c8lA Hybrid designing of potent inhibitors of striga strigolactone receptor shhtl7
    24% identity, 85% coverage of query (55.5 bits)

NAP_BACSU / P96688 Uncharacterized carboxylesterase nap; EC 3.1.1.1 from Bacillus subtilis (strain 168)
    24% identity, 71% coverage of query (55.1 bits)

5dnuA / A0A0M3PN85 Crystal structure of striga kai2-like protein in complex with karrikin (see paper)
    22% identity, 88% coverage of query (54.7 bits)

3bwxA / Q2G9T7 Crystal structure of an alpha/beta hydrolase (yp_496220.1) from novosphingobium aromaticivorans dsm 12444 at 1.50 a resolution
    23% identity, 96% coverage of query (54.7 bits)

D0EPY0 carboxylesterase (EC 3.1.1.1) from Bacillus subtilis (see paper)
    23% identity, 71% coverage of query (54.3 bits)

4ccwA / Q59248 Crystal structure of naproxen esterase (carboxylesterase np) from bacillus subtilis (see paper)
    23% identity, 70% coverage of query (53.9 bits)

5z89A Structural basis for specific inhibition of highly sensitive shhtl7 receptor
    24% identity, 86% coverage of query (53.5 bits)

7k38A Crystal structure of pisum sativum kai2 in complex with gr24-ent5ds product (see paper)
    20% identity, 88% coverage of query (53.1 bits)

Q5FMT1 prolyl aminopeptidase (EC 3.4.11.5) from Lactobacillus acidophilus (see paper)
    24% identity, 93% coverage of query (53.1 bits)

D14_ARATH / Q9SQR3 Strigolactone esterase D14; Protein DWARF 14; AtD14; EC 3.1.-.- from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
    22% identity, 92% coverage of query (52.8 bits)

RSBQ_BACSU / O07015 Sigma factor SigB regulation protein RsbQ from Bacillus subtilis (strain 168) (see 2 papers)
    21% identity, 93% coverage of query (52.4 bits)

6i8wB / Q9KJG6 Crystal structure of a membrane phospholipase a, a novel bacterial virulence factor (see paper)
    24% identity, 91% coverage of query (50.8 bits)

6o5jA Crystal structure of dad2 bound to quinazolinone derivative
    23% identity, 92% coverage of query (50.4 bits)

6ap7A Crystal structure of dad2 in complex with 2-(2-methyl-3-nitroanilino) benzoic acid
    23% identity, 92% coverage of query (50.4 bits)

6ap6A / J9U5U9 Crystal structure of dad2 in complex with tolfenamic acid (see paper)
    23% identity, 92% coverage of query (50.4 bits)

1m33A / P13001 Crystal structure of bioh at 1.7 a (see paper)
    23% identity, 84% coverage of query (49.3 bits)

1hl7A / Q8GJP7 Gamma lactamase from an aureobacterium species in complex with 3a,4,7, 7a-tetrahydro-benzo [1,3] dioxol-2-one (see paper)
    25% identity, 88% coverage of query (48.5 bits)

5hzgA / Q9SQR3 The crystal structure of the strigolactone-induced atd14-d3-ask1 complex (see paper)
    24% identity, 92% coverage of query (48.5 bits)

BioB / b3412 pimeloyl-acyl carrier protein methyl ester esterase (EC 3.1.1.85; EC 3.1.1.1) from Escherichia coli K-12 substr. MG1655 (see 8 papers)
bioH / P13001 pimeloyl-acyl carrier protein methyl ester esterase (EC 3.1.1.85; EC 3.1.1.1) from Escherichia coli (strain K12) (see 8 papers)
BIOH_ECOLI / P13001 Pimeloyl-[acyl-carrier protein] methyl ester esterase; Biotin synthesis protein BioH; Carboxylesterase BioH; EC 3.1.1.85 from Escherichia coli (strain K12) (see 4 papers)
P13001 carboxylesterase (EC 3.1.1.1); pimelyl-[acyl-carrier protein] methyl ester esterase (EC 3.1.1.85) from Escherichia coli (see paper)
    22% identity, 84% coverage of query (48.1 bits)

DAD2_PETHY / J9U5U9 Probable strigolactone esterase DAD2; Protein DECREASED APICAL DOMINANCE 2; EC 3.1.-.- from Petunia hybrida (Petunia) (see paper)
    22% identity, 92% coverage of query (47.0 bits)

4etwA Structure of the enzyme-acp substrate gatekeeper complex required for biotin synthesis
    22% identity, 89% coverage of query (47.0 bits)

RMS3_PEA / A0A109QYD3 Strigolactone esterase RMS3; Protein DWARF 14 homolog; PsD14; Protein RAMOSUS 3; EC 3.1.-.- from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see paper)
    21% identity, 85% coverage of query (46.2 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory