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Searching for up to 100 curated homologs for WP_004687247.1 NCBI__GCF_000022745.1:WP_004687247.1 (389 a.a.)

Found high-coverage hits (≥70%) to 42 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

pobA / AAA73519.1 4-hydroxybenzoate hydroxylase from Rhizobium leguminosarum (see paper)
    78% identity, 100% coverage of query (637 bits)

1pbcA Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2-hydroxy- 4-aminobenzoate and of the try222ala mutant, complexed with 2- hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring
    64% identity, 100% coverage of query (524 bits)

1bf3A P-hydroxybenzoate hydroxylase (phbh) mutant with cys 116 replaced by ser (c116s) and arg 42 replaced by lys (r42k), in complex with fad and 4-hydroxybenzoic acid
    64% identity, 100% coverage of query (523 bits)

pobA / P00438 p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas fluorescens (see 2 papers)
PHHY_PSEFL / P00438 p-hydroxybenzoate hydroxylase; PHBH; PHBHase; 4-hydroxybenzoate 3-monooxygenase; EC 1.14.13.2 from Pseudomonas fluorescens (see 13 papers)
    64% identity, 100% coverage of query (522 bits)

2phhA The coenzyme analogue adenosine 5-diphosphoribose displaces fad in the active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation
    64% identity, 100% coverage of query (522 bits)

1pdhA Crystal structure of p-hydroxybenzoate hydroxylase reconstituted with the modified fad present in alcohol oxidase from methylotrophic yeasts: evidence for an arabinoflavin
    64% identity, 100% coverage of query (522 bits)

PHHY_PSEAE / P20586 p-hydroxybenzoate hydroxylase; PHBH; 4-hydroxybenzoate 3-monooxygenase; EC 1.14.13.2 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 7 papers)
1d7lA / P20586 Structure-function correlations of the reaction of reduced nicotinamide analogs with p-hydroxybenzoate hydroxylase substituted with a series of 8-substituted flavins (see paper)
    63% identity, 100% coverage of query (521 bits)

1iusA P-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate at ph 5.0
    63% identity, 100% coverage of query (521 bits)

1dodA The mobil flavin of 4-oh benzoate hydroxylase: motion of a prosthetic group regulates catalysis
    63% identity, 100% coverage of query (521 bits)

1k0lA Pseudomonas aeruginosa phbh r220q free of p-ohb
    63% identity, 100% coverage of query (520 bits)

1k0jA Pseudomonas aeruginosa phbh r220q in complex with NADPH and free of p- ohb
    63% identity, 100% coverage of query (520 bits)

8y2sA 4-hydroxybenzoate 3-monooxygenase
    63% identity, 100% coverage of query (515 bits)

1ykjB A45g p-hydroxybenzoate hydroxylase with p-hydroxybenzoate bound
    63% identity, 100% coverage of query (512 bits)

pobA / Q9R9T1 p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas putida (see paper)
Q9R9T1 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Pseudomonas putida (see paper)
    62% identity, 100% coverage of query (508 bits)

Pf6N2E2_2914 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Pseudomonas fluorescens FW300-N2E2
    62% identity, 100% coverage of query (507 bits)

6dllB / Q88H28 2.2 angstrom resolution crystal structure of p-hydroxybenzoate hydroxylase from pseudomonas putida in complex with fad. (see paper)
    62% identity, 100% coverage of query (506 bits)

RR42_RS21940 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Cupriavidus basilensis FW507-4G11
    65% identity, 100% coverage of query (501 bits)

pobA / F2JUE7 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) from Marinomonas mediterranea (strain ATCC 700492 / JCM 21426 / NBRC 103028 / MMB-1) (see paper)
    57% identity, 100% coverage of query (464 bits)

7on9A / C4TP09 Crystal structure of para-hydroxybenzoate-3-hydroxylase prai (see paper)
    52% identity, 100% coverage of query (401 bits)

PRAI_PAESP / C4TP09 4-hydroxybenzoate 3-monooxygenase (NAD(P)H); 4-hydroxybenzoate 3-hydroxylase; 4HB 3-hydroxylase; EC 1.14.13.33 from Paenibacillus sp. (see paper)
praI / BAH79107.1 4-hydroxybenzoate 3-hydroxylase from Paenibacillus sp. JJ-1b (see paper)
    52% identity, 100% coverage of query (401 bits)

8jqoA Protocatecuate hydroxylase from xylophilus ampelinus complexed with imidazole (see paper)
    44% identity, 100% coverage of query (344 bits)

8weqC / A0A4P7SP66 P-hydroxybenzoate 3-monooxygenase
    46% identity, 100% coverage of query (337 bits)

8jqoD Protocatecuate hydroxylase from xylophilus ampelinus complexed with imidazole (see paper)
    38% identity, 99% coverage of query (255 bits)

Build an alignment

Build an alignment for WP_004687247.1 and 23 homologs with ≥ 30% identity

Select sequences

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Change minimum %identity:

Additional hits (identity < 30%)

7yj0D Structural basis of oxepinone formation by a flavin-monooxygenase vibo (see paper)
    26% identity, 79% coverage of query (72.4 bits)

NTNA_NECSZ / A0A455LLV4 FAD-dependent monooxygenase ntnA; Nectripenoid biosynthesis cluster protein A; EC 1.-.-.- from Nectria sp. (see paper)
    24% identity, 80% coverage of query (58.9 bits)

pqsH / Q9I0Q0 2-heptyl-3-hydroxy-4(1H)-quinolone synthase (EC 1.14.13.182) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
PQSH_PSEAE / Q9I0Q0 2-heptyl-3-hydroxy-4(1H)-quinolone synthase; 2-heptyl-3,4-dihydroxyquinoline synthase; EC 1.14.13.182 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
Q9I0Q0 2-heptyl-3-hydroxy-4(1H)-quinolone synthase (EC 1.14.13.182) from Pseudomonas aeruginosa (see paper)
    28% identity, 78% coverage of query (58.2 bits)

7oujAAA / Q8KY42 7oujAAA (see paper)
    26% identity, 75% coverage of query (57.0 bits)

6brdA / F2R776 Crystal structure of rifampin monooxygenase from streptomyces venezuelae, complexed with rifampin and fad (see paper)
    23% identity, 86% coverage of query (56.6 bits)

6ui5A / Q1MX79 Tmn9 in complex with cofactor fad
    26% identity, 78% coverage of query (56.6 bits)

ROX_STRVP / F2R776 Rifampicin monooxygenase; RIFMO; EC 1.14.13.211 from Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745) (see paper)
    23% identity, 86% coverage of query (56.2 bits)

PQSH_PSEAB / Q02N79 2-heptyl-3-hydroxy-4(1H)-quinolone synthase; 2-heptyl-3,4-dihydroxyquinoline synthase; EC 1.14.13.182 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see 2 papers)
    26% identity, 78% coverage of query (55.5 bits)

TERD_ASPTN / Q0D1P2 FAD-dependent monooxygenase terD; Terrein biosynthesis cluster protein terD; EC 1.-.-.- from Aspergillus terreus (strain NIH 2624 / FGSC A1156) (see 13 papers)
    22% identity, 77% coverage of query (53.9 bits)

vppD / A0A075XAG5 6-hydroxy-3-succinoyl-pyridine hydroxylase (EC 1.14.13.163) from Ochrobactrum sp. SJY1 (see 2 papers)
A0A075XAG5 6-hydroxy-3-succinoylpyridine 3-monooxygenase (EC 1.14.13.163) from Ochrobactrum sp. (see paper)
    26% identity, 80% coverage of query (53.1 bits)

mtmOII / Q194R1 MtmOII from Streptomyces argillaceus (see 3 papers)
    25% identity, 89% coverage of query (52.4 bits)

PYRE3_STRRG / K7QRJ5 Dialkyldecalin synthase; FAD-dependent [4+2] cyclase; EC 5.5.1.- from Streptomyces rugosporus (see 2 papers)
    26% identity, 79% coverage of query (52.4 bits)

ROX_NOCFA / Q5YTV5 Rifampicin monooxygenase; RIFMO; EC 1.14.13.211 from Nocardia farcinica (strain IFM 10152) (see 3 papers)
Q5YTV5 rifampicin monooxygenase (EC 1.14.13.211) from Nocardia farcinica (see paper)
    28% identity, 78% coverage of query (52.4 bits)

W8E0Y0 6-hydroxy-3-succinoylpyridine 3-monooxygenase (EC 1.14.13.163) from Agrobacterium tumefaciens (see 2 papers)
    25% identity, 80% coverage of query (52.4 bits)

5kowA / Q5YTV5 Structure of rifampicin monooxygenase (see paper)
    28% identity, 78% coverage of query (52.4 bits)

6c7sA Structure of rifampicin monooxygenase with product bound
    28% identity, 78% coverage of query (52.4 bits)

5koxA Structure of rifampicin monooxygenase complexed with rifampicin
    28% identity, 78% coverage of query (52.4 bits)

5xgvA / K7QRJ5 The structure of diels-alderase pyre3 in the biosynthetic pathway of pyrroindomycins (see paper)
    26% identity, 78% coverage of query (51.2 bits)

8fhjC Crystal structure of a fad monooxygenease from methylocystis sp. Strain sb2 (see paper)
    24% identity, 89% coverage of query (47.8 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory