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Searching for up to 100 curated homologs for WP_006748146.1 NCBI__GCF_000227685.2:WP_006748146.1 (595 a.a.)

Found high-coverage hits (≥70%) to 39 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

asnO / Q92W82 N-acetylglutaminylglutamine amide synthase from Rhizobium meliloti (strain 1021) (see paper)
    52% identity, 99% coverage of query (592 bits)

ddaH / E2JA33 N-3-fumaroyl-(S)-2,3-diaminopropanoate aminotransferase from Enterobacter agglomerans (see 2 papers)
    33% identity, 100% coverage of query (284 bits)

oxyD / L8EVF2 malonamoyl-CoA synthetase from Streptomyces rimosus subsp. rimosus (strain ATCC 10970 / DSM 40260 / JCM 4667 / NRRL 2234) (see 3 papers)
    37% identity, 88% coverage of query (271 bits)

asnB / GB|CAB15032.1 asparagine synthase (glutamine-hydrolysing) 1; EC 6.3.5.4 from Bacillus subtilis (see 4 papers)
    30% identity, 100% coverage of query (264 bits)

asnO / GI|1934835 asparagine synthase (glutamine-hydrolysing) 3; EC 6.3.5.4 from Bacillus subtilis (see 3 papers)
    30% identity, 99% coverage of query (257 bits)

ltsA / BAD83576.1 glutamine amidotransferase from Rhodococcus erythropolis (see paper)
    33% identity, 87% coverage of query (246 bits)

ASNH_MYCBO / P64248 Putative asparagine synthetase [glutamine-hydrolyzing]; EC 6.3.5.4 from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) (see paper)
    34% identity, 95% coverage of query (245 bits)

Build an alignment

Build an alignment for WP_006748146.1 and 7 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

asnH / Q9CDJ2 peptidoglycan asparagine synthase from Lactococcus lactis subsp. lactis (strain IL1403) (see paper)
    29% identity, 89% coverage of query (223 bits)

AsnB / b0674 asparagine synthetase B (EC 6.3.5.4; EC 6.3.1.1; EC 3.5.1.38; EC 3.5.1.2) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
asnB / P22106 asparagine synthetase B (EC 6.3.5.4; EC 6.3.1.1; EC 3.5.1.38) from Escherichia coli (strain K12) (see 17 papers)
ASNB_ECOLI / P22106 Asparagine synthetase B [glutamine-hydrolyzing]; AS-B; EC 6.3.5.4 from Escherichia coli (strain K12) (see 6 papers)
asnB asparagine synthetase B, glutamine-hydrolyzing; EC 6.3.5.4 from Escherichia coli (see paper)
    29% identity, 88% coverage of query (182 bits)

1ct9A / P22106 Crystal structure of asparagine synthetase b from escherichia coli (see paper)
    30% identity, 84% coverage of query (182 bits)

B3KYI2 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Phaseolus vulgaris (see paper)
    28% identity, 88% coverage of query (164 bits)

Q10MX3 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Oryza sativa Japonica Group (see paper)
    28% identity, 88% coverage of query (161 bits)

Q6HA26 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Pinus sylvestris (see paper)
    29% identity, 90% coverage of query (161 bits)

Q14SM9 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Glycine max (see paper)
    29% identity, 88% coverage of query (160 bits)

E7EAQ2 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Pinus pinaster (see paper)
    29% identity, 88% coverage of query (160 bits)

F2DJ31 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare (see paper)
    28% identity, 88% coverage of query (160 bits)

F2CQ60 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare subsp. vulgare (see paper)
    28% identity, 88% coverage of query (160 bits)

Q9SP19 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Helianthus annuus (see paper)
    29% identity, 88% coverage of query (159 bits)

ASNS2_ARATH / Q9LV77 Asparagine synthetase [glutamine-hydrolyzing] 2; Glutamine-dependent asparagine synthetase 2; EC 6.3.5.4 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
    28% identity, 88% coverage of query (156 bits)

ASNS2_ORYSJ / Q43011 Asparagine synthetase [glutamine-hydrolyzing] 2; Glutamine-dependent asparagine synthetase 2; EC 6.3.5.4 from Oryza sativa subsp. japonica (Rice) (see paper)
Q43011 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Oryza sativa Japonica Group (see paper)
    28% identity, 88% coverage of query (155 bits)

ASNS1_ARATH / P49078 Asparagine synthetase [glutamine-hydrolyzing] 1; Glutamine-dependent asparagine synthetase 1; Protein DARK INDUCIBLE 6; EC 6.3.5.4 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
    27% identity, 88% coverage of query (152 bits)

Q9SP20 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Helianthus annuus (see paper)
    28% identity, 88% coverage of query (152 bits)

A0A1D5VHV8 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Triticum aestivum (see paper)
    28% identity, 88% coverage of query (152 bits)

ASNS_SCHPO / P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
asn1 / RF|NP_595291.1 asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (see 2 papers)
    29% identity, 84% coverage of query (151 bits)

D9IXC8 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Pinus pinaster (see paper)
    28% identity, 88% coverage of query (150 bits)

Q9LKQ9 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Helianthus annuus (see paper)
    28% identity, 88% coverage of query (150 bits)

Q93XP9 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare subsp. vulgare (see paper)
    28% identity, 83% coverage of query (150 bits)

A0A1D5YL35 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Triticum aestivum (see paper)
    28% identity, 83% coverage of query (149 bits)

A9XS73 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Phaseolus vulgaris (see paper)
    28% identity, 83% coverage of query (148 bits)

F2DDF6 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare subsp. vulgare (see paper)
    27% identity, 90% coverage of query (147 bits)

Asns / P49088 asparagine synthetase subunit (EC 6.3.5.4) from Rattus norvegicus (see paper)
    26% identity, 92% coverage of query (144 bits)

F2CWZ7 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare subsp. vulgare (see paper)
    27% identity, 88% coverage of query (144 bits)

Q84LA5 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare (see paper)
    27% identity, 88% coverage of query (144 bits)

ASNS_MOUSE / Q61024 Asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Mus musculus (Mouse) (see paper)
    26% identity, 92% coverage of query (144 bits)

ASN1 asparagine synthetase from Candida albicans (see paper)
    26% identity, 97% coverage of query (140 bits)

Q9SM55 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Phaseolus vulgaris (see paper)
    26% identity, 83% coverage of query (139 bits)

ASNS_HUMAN / P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 2 papers)
P08243 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Homo sapiens (see 4 papers)
    25% identity, 92% coverage of query (135 bits)

Q2L9S5 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Bombyx mori (see paper)
    27% identity, 88% coverage of query (128 bits)

6gq3A / P08243 Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
    25% identity, 87% coverage of query (127 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory