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Searching for up to 100 curated homologs for WP_010963584.1 NCBI__GCF_000008765.1:WP_010963584.1 (265 a.a.)

Found high-coverage hits (≥70%) to 76 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

P58637 homocitrate synthase (EC 2.3.3.14) from Nostoc sp. PCC 7120 = FACHB-418 (see paper)
    43% identity, 93% coverage of query (210 bits)

A0A0S6UXF5 homocitrate synthase (EC 2.3.3.14) from Bradyrhizobium sp. DOA9 (see paper)
    41% identity, 86% coverage of query (189 bits)

cimA / Q58787 (R)-citratemalate synthase subunit (EC 2.3.3.21) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see paper)
CIMA_METJA / Q58787 (R)-citramalate synthase CimA; EC 2.3.3.21 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58787 (R)-citramalate synthase (EC 2.3.3.21) from Methanocaldococcus jannaschii (see 2 papers)
    39% identity, 99% coverage of query (188 bits)

A0A1Y6KBA1 homocitrate synthase (EC 2.3.3.14) from Bradyrhizobium sp. ORS 285 (see 2 papers)
    40% identity, 86% coverage of query (185 bits)

nifV / P05342 homocitrate synthase monomer (EC 2.3.3.14) from Azotobacter vinelandii (see paper)
NIFV_AZOVI / P05342 Homocitrate synthase; EC 2.3.3.14 from Azotobacter vinelandii (see paper)
    38% identity, 84% coverage of query (179 bits)

LEU1_METJA / Q58595 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58595 2-isopropylmalate synthase (EC 2.3.3.13) from Methanocaldococcus jannaschii (see paper)
    41% identity, 93% coverage of query (178 bits)

LEU1_SULAC / Q4JA78 2-isopropylmalate synthase; IPMS; Alpha-isopropylmalate synthase; Alpha-IPM synthase; EC 2.3.3.13 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
    38% identity, 87% coverage of query (158 bits)

HOSA_SULTO / Q971S5 Homocitrate synthase; HCS; EC 2.3.3.14 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
    39% identity, 86% coverage of query (157 bits)

frbC / Q0ZQ46 2-phosphonomethylmalate synthase (EC 2.3.3.19) from Streptomyces rubellomurinus (strain ATCC 31215) (see 2 papers)
FRBC_STRR3 / Q0ZQ46 2-phosphonomethylmalate synthase; EC 2.3.3.19 from Streptomyces rubellomurinus (strain ATCC 31215) (see paper)
Q0ZQ46 2-phosphonomethylmalate synthase (EC 2.3.3.19) from Streptomyces rubellomurinus (see paper)
    34% identity, 93% coverage of query (155 bits)

HOSA_SULAC / Q4J989 Homocitrate synthase; HCS; EC 2.3.3.14 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
Q4J989 homocitrate synthase (EC 2.3.3.14) from Sulfolobus acidocaldarius (see paper)
    37% identity, 86% coverage of query (149 bits)

6ktqA / Q4J989 Crystal structure of catalytic domain of homocitrate synthase from sulfolobus acidocaldarius (sahcs(dram)) in complex with alpha- ketoglutarate/zn2+/coa (see paper)
    37% identity, 86% coverage of query (149 bits)

AKSA_METJA / Q57926 Homocitrate synthase AksA; (R)-homo(2)citrate synthase; (R)-homo(3)citrate synthase; EC 2.3.3.14; EC 2.3.3.- from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
    37% identity, 86% coverage of query (137 bits)

3ivtB / Q9Y823 Homocitrate synthase lys4 bound to 2-og (see paper)
    36% identity, 84% coverage of query (134 bits)

HOSM_SCHPO / Q9Y823 Homocitrate synthase, mitochondrial; HCS; EC 2.3.3.14 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
lys4 / RF|NP_596458.1 homocitrate synthase (predicted); EC 2.3.3.14 from Schizosaccharomyces pombe (see 2 papers)
    36% identity, 84% coverage of query (134 bits)

3rmjB / Q9JZG1 Crystal structure of truncated alpha-isopropylmalate synthase from neisseria meningitidis (see paper)
    35% identity, 85% coverage of query (130 bits)

LYS21 / Q12122 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 6 papers)
HOSM_YEAST / Q12122 Homocitrate synthase, mitochondrial; HCS; EC 2.3.3.14 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
Q12122 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (see paper)
    34% identity, 85% coverage of query (130 bits)

LYS20 / P48570 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 6 papers)
HOSC_YEAST / P48570 Homocitrate synthase, cytosolic isozyme; HCS; EC 2.3.3.14 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
P48570 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (see 2 papers)
    34% identity, 85% coverage of query (129 bits)

LEU1_NEIMB / Q9JZG1 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Neisseria meningitidis serogroup B (strain MC58) (see 2 papers)
Q9JZG1 2-isopropylmalate synthase (EC 2.3.3.13) from Neisseria meningitidis (see 2 papers)
    35% identity, 86% coverage of query (129 bits)

3mi3A Homocitrate synthase lys4 bound to lysine
    36% identity, 85% coverage of query (128 bits)

LEU12_ARATH / Q9C550 2-isopropylmalate synthase 2, chloroplastic; 2-isopropylmalate synthase 1; Methylthioalkylmalate synthase-like 3; EC 2.3.3.13 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9C550 2-isopropylmalate synthase (EC 2.3.3.13) from Arabidopsis thaliana (see 2 papers)
    35% identity, 86% coverage of query (127 bits)

Q72JC9 2-isopropylmalate synthase (EC 2.3.3.13) from Thermus thermophilus (see 2 papers)
    34% identity, 86% coverage of query (126 bits)

C7C437 homocitrate synthase (EC 2.3.3.14) from Aspergillus fumigatus (see paper)
    34% identity, 85% coverage of query (126 bits)

LEU11_ARATH / Q9LPR4 2-isopropylmalate synthase 1, chloroplastic; Methylthioalkylmalate synthase-like 4; EC 2.3.3.13 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
Q9LPR4 2-isopropylmalate synthase (EC 2.3.3.13) from Arabidopsis thaliana (see 3 papers)
    35% identity, 86% coverage of query (125 bits)

M1U949 homocitrate synthase (EC 2.3.3.14) from Flammulina velutipes (see paper)
    32% identity, 97% coverage of query (125 bits)

3ivsA Homocitrate synthase lys4
    35% identity, 84% coverage of query (125 bits)

K4CJ56 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum lycopersicum (see paper)
    33% identity, 89% coverage of query (123 bits)

Q30DX9 2-isopropylmalate synthase (EC 2.3.3.13) from Brassica insularis (see paper)
    35% identity, 86% coverage of query (123 bits)

A0A0G2T6D7 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum pennellii (see paper)
    33% identity, 89% coverage of query (123 bits)

CA265_RS15855 2-isopropylmalate synthase (EC 2.3.3.13) from Pedobacter sp. GW460-11-11-14-LB5
    31% identity, 99% coverage of query (123 bits)

MAM3 / Q9FN52 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see paper)
MAM3_ARATH / Q9FN52 Methylthioalkylmalate synthase 3, chloroplastic; 2-isopropylmalate synthase 2; Methylthioalkylmalate synthase-like; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9FN52 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see 4 papers)
    33% identity, 86% coverage of query (122 bits)

MAM1 / Q9FG67 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see 2 papers)
MAM1_ARATH / Q9FG67 Methylthioalkylmalate synthase 1, chloroplastic; 2-isopropylmalate synthase 3; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
Q9FG67 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see 7 papers)
    33% identity, 86% coverage of query (121 bits)

Echvi_3833 2-isopropylmalate synthase (EC 2.3.3.13) from Echinicola vietnamensis KMM 6221, DSM 17526
    32% identity, 98% coverage of query (121 bits)

K4C627 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum lycopersicum (see paper)
    33% identity, 86% coverage of query (120 bits)

CIRSY_DEHMC / P0DO96 Citrate (Re)-synthase; Re face-specific citrate synthase; Re-citrate synthase; EC 2.3.3.3 from Dehalococcoides mccartyi (strain CBDB1) (see paper)
P0DO96 citrate (Re)-synthase (EC 2.3.3.3) from Dehalococcoides sp. (see paper)
    35% identity, 89% coverage of query (120 bits)

MAM2_ARATH / Q8VX04 Methylthioalkylmalate synthase 2, chloroplastic; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q8VX04 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see paper)
    33% identity, 86% coverage of query (119 bits)

BT1861 2-isopropylmalate synthase (EC 2.3.3.13) from Bacteroides thetaiotaomicron VPI-5482
    31% identity, 98% coverage of query (119 bits)

LEU1_SOYBN / Q39891 Probable 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; Late nodulin-56; N-56; EC 2.3.3.13 from Glycine max (Soybean) (Glycine hispida) (see paper)
    33% identity, 86% coverage of query (117 bits)

D0VY45 homocitrate synthase (EC 2.3.3.14) from Lotus japonicus (see paper)
    33% identity, 86% coverage of query (116 bits)

3a9iA / O87198 Crystal structure of homocitrate synthase from thermus thermophilus complexed with lys (see paper)
    35% identity, 88% coverage of query (114 bits)

2ztjA Crystal structure of homocitrate synthase from thermus thermophilus complexed with alpha-ketoglutarate
    35% identity, 88% coverage of query (114 bits)

LEU11_LEPIN / Q8F445 2-isopropylmalate synthase 1; Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1; EC 2.3.3.13 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see paper)
    35% identity, 85% coverage of query (113 bits)

6e1jA / C5J4P1 Crystal structure of methylthioalkylmalate synthase (bjumam1.1) from brassica juncea (see paper)
    30% identity, 98% coverage of query (113 bits)

LeuA / b0074 2-isopropylmalate synthase (EC 2.3.3.13) from Escherichia coli K-12 substr. MG1655 (see 7 papers)
leuA / P09151 2-isopropylmalate synthase (EC 2.3.3.13) from Escherichia coli (strain K12) (see 15 papers)
    33% identity, 88% coverage of query (112 bits)

LEU1_SALTY / P15875 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 3 papers)
    32% identity, 86% coverage of query (112 bits)

3bliA / Q8F3Q1 Crystal structure of the catalytic domain of licms in complexed with pyruvate and acetyl-coa (see paper)
    30% identity, 91% coverage of query (112 bits)

cimA / Q8F3Q1 (R)-citramalate synthase subunit (EC 2.3.3.21) from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see paper)
CIMA_LEPIN / Q8F3Q1 (R)-citramalate synthase CimA; LiCMS; EC 2.3.3.21 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see 3 papers)
    30% identity, 91% coverage of query (112 bits)

2zyfA Crystal structure of homocitrate synthase from thermus thermophilus complexed with magnesuim ion and alpha-ketoglutarate
    35% identity, 88% coverage of query (112 bits)

hcs / O87198 homocitrate synthase (EC 2.3.3.14) from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
HOSA_THET2 / O87198 Homocitrate synthase; HCS; EC 2.3.3.14 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 3 papers)
    33% identity, 88% coverage of query (111 bits)

Build an alignment

Build an alignment for WP_010963584.1 and 48 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

Q2RAP8 2-isopropylmalate synthase (EC 2.3.3.13) from Oryza sativa Japonica Group (see paper)
    28% identity, 98% coverage of query (110 bits)

Q2QXY9 2-isopropylmalate synthase (EC 2.3.3.13) from Oryza sativa Japonica Group (see paper)
    28% identity, 98% coverage of query (110 bits)

B0K6M2 (R)-citramalate synthase (EC 2.3.3.21) from Thermoanaerobacter sp. (see paper)
    29% identity, 88% coverage of query (100 bits)

hphA / B2J8P8 2-benzylmalate synthase from Nostoc punctiforme (strain ATCC 29133 / PCC 73102) (see paper)
    29% identity, 89% coverage of query (97.8 bits)

CIMA_SULAC / Q4J6H1 (R)-citramalate synthase; Citramalate synthase; CMS; EC 2.3.3.21 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
    31% identity, 96% coverage of query (97.4 bits)

BT1858 (R)-citramalate synthase (EC 2.3.1.182) from Bacteroides thetaiotaomicron VPI-5482
    30% identity, 85% coverage of query (94.7 bits)

B0SN40 2-isopropylmalate synthase (EC 2.3.3.13) from Leptospira biflexa (see paper)
    30% identity, 86% coverage of query (87.0 bits)

4ov9A / B0SN40 Structure of isopropylmalate synthase binding with alpha- isopropylmalate (see paper)
    30% identity, 86% coverage of query (87.0 bits)

4ov4A Isopropylmalate synthase binding with ketoisovalerate
    30% identity, 86% coverage of query (87.0 bits)

LEU12_LEPIN / Q8F8T4 2-isopropylmalate synthase 2; Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2; EC 2.3.3.13 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see 2 papers)
    30% identity, 85% coverage of query (84.7 bits)

cimA / Q74C76 (R)-citramalate synthase (EC 2.3.3.21) from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) (see paper)
CIMA_GEOSL / Q74C76 (R)-citramalate synthase; Citramalate synthase; EC 2.3.3.21 from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) (see paper)
Q74C76 (R)-citramalate synthase (EC 2.3.3.21) from Geobacter sulfurreducens (see paper)
    29% identity, 88% coverage of query (79.7 bits)

DVU1914 (R)-citramalate synthase (EC 2.3.3.21) from Desulfovibrio vulgaris Hildenborough JW710
    27% identity, 89% coverage of query (77.4 bits)

CIRSY_CLOK5 / A5N6T4 Citrate (Re)-synthase; Re-citrate synthase; EC 2.3.3.3 from Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) (see paper)
    28% identity, 86% coverage of query (72.8 bits)

HOA_THET8 / Q53WI0 4-hydroxy-2-oxovalerate aldolase; HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxohexanoate aldolase; 4-hydroxy-2-oxopentanoate aldolase; EC 4.1.3.39; EC 4.1.3.43 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
Q53WI0 4-hydroxy-2-oxohexanoate aldolase (EC 4.1.3.43) from Thermus thermophilus (see paper)
    24% identity, 90% coverage of query (71.2 bits)

rhiG / D4HRI4 rhiG aldolase subunit from Bacillus subtilis subsp. spizizenii ATCC 6633 (see paper)
    25% identity, 90% coverage of query (71.2 bits)

CIRSY_SYNAS / Q2LTE1 Citrate (Re)-synthase; Re-citrate synthase; EC 2.3.3.3 from Syntrophus aciditrophicus (strain SB) (see paper)
    25% identity, 91% coverage of query (69.7 bits)

P51017 4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.39) from Pseudomonas putida (see paper)
nahM / AAA89107.1 4-hydroxy-2-oxovalerate aldolase from Pseudomonas putida (see paper)
    26% identity, 89% coverage of query (60.1 bits)

nahM / BAE92172.1 4-hydroxy-2-oxovalerate aldolase NahM from Pseudomonas putida (see 2 papers)
    25% identity, 89% coverage of query (59.3 bits)

pmmS / Q9LCB4 2-phosphinomethylmalate synthase monomer (EC 2.3.3.18) from Streptomyces hygroscopicus (see paper)
PMMS_STRHY / Q9LCB4 2-phosphinomethylmalate synthase; PMS; PMM synthase; EC 2.3.3.18 from Streptomyces hygroscopicus (see 2 papers)
Q9LCB4 2-phosphinomethylmalate synthase (EC 2.3.3.18); 2-phosphonomethylmalate synthase (EC 2.3.3.19) from Streptomyces hygroscopicus (see 4 papers)
    25% identity, 87% coverage of query (58.2 bits)

pms / A0A0M3WP31 2-phosphinomethylmalate synthase (EC 2.3.3.18) from Kitasatospora phosalacinea (see 2 papers)
    23% identity, 90% coverage of query (55.5 bits)

HMGC2_HUMAN / Q8TB92 3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic; 3-hydroxy-3-methylglutaryl-CoA lyase-like protein 1; HMGCL-like 1; Endoplasmic reticulum 3-hydroxy-3-methylglutaryl-CoA lyase; er-cHL; EC 4.1.3.4 from Homo sapiens (Human) (see 2 papers)
    22% identity, 87% coverage of query (51.6 bits)

HMGCL / P35914 Hydroxymethylglutaryl-CoA lyase, mitochondrial (EC 4.1.3.4) from Homo sapiens (see 9 papers)
HMGCL_HUMAN / P35914 Hydroxymethylglutaryl-CoA lyase, mitochondrial; HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; EC 4.1.3.4 from Homo sapiens (Human) (see 18 papers)
P35914 hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) from Homo sapiens (see 4 papers)
    24% identity, 93% coverage of query (50.1 bits)

3mp3B Crystal structure of human lyase in complex with inhibitor hg-coa
    24% identity, 93% coverage of query (50.1 bits)

2cw6A / P35914 Crystal structure of human hmg-coa lyase: insights into catalysis and the molecular basis for hydroxymethylglutaric aciduria (see paper)
    24% identity, 93% coverage of query (50.1 bits)

5ks8F / Q1H157 Crystal structure of two-subunit pyruvate carboxylase from methylobacillus flagellatus (see paper)
    25% identity, 85% coverage of query (48.5 bits)

5ks8D / Q1H157 Crystal structure of two-subunit pyruvate carboxylase from methylobacillus flagellatus (see paper)
    25% identity, 85% coverage of query (48.1 bits)

5ks8C / Q1H157 Crystal structure of two-subunit pyruvate carboxylase from methylobacillus flagellatus (see paper)
    25% identity, 85% coverage of query (47.4 bits)

6ndsA / A0A0D5YK08 Structure of an hmg-coa lyase from acenitobacter baumannii in complex with coenzyme a and 3-methylmalate
    22% identity, 84% coverage of query (46.6 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory