Sites on a Tree

 

Searching for up to 100 curated homologs for WP_011384016.1 NCBI__GCF_000009985.1:WP_011384016.1 (275 a.a.)

Found high-coverage hits (≥70%) to 79 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

NIT1_SYNYG / P0DP66 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; syNit1; EC 3.5.1.128 from Synechocystis sp. (strain PCC 6803 / GT-S) (see paper)
P0DP66 deaminated glutathione amidase (EC 3.5.1.128) from Synechocystis sp. PCC 6803 (see paper)
    43% identity, 97% coverage of query (213 bits)

NILP2_ARATH / Q94JV5 Deaminated glutathione amidase, chloroplastic/cytosolic; dGSH amidase; Nitrilase-like protein 2; Protein nitrilase 1 homolog; AtNit1; Protein Nit1 homolog; EC 3.5.1.128 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q94JV5 deaminated glutathione amidase (EC 3.5.1.128) from Arabidopsis thaliana (see paper)
    41% identity, 97% coverage of query (202 bits)

Q9HVU6 deaminated glutathione amidase (EC 3.5.1.128) from Pseudomonas aeruginosa (see paper)
    40% identity, 96% coverage of query (189 bits)

NIT1_YEREN / P0DP68 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; yeNit1; EC 3.5.1.128 from Yersinia enterocolitica (see paper)
P0DP68 deaminated glutathione amidase (EC 3.5.1.128) from Yersinia enterocolitica (see paper)
    35% identity, 97% coverage of query (187 bits)

NIT1_HUMAN / Q86X76 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Homo sapiens (Human) (see paper)
    38% identity, 96% coverage of query (184 bits)

NIT1_MOUSE / Q8VDK1 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Mus musculus (Mouse) (see 7 papers)
Q8VDK1 deaminated glutathione amidase (EC 3.5.1.128) from Mus musculus (see paper)
    38% identity, 96% coverage of query (181 bits)

NFT1_CAEEL / O76463 Nitrilase and fragile histidine triad fusion protein NitFhit; EC 3.6.1.29; EC 3.5.-.- from Caenorhabditis elegans (see 2 papers)
    39% identity, 97% coverage of query (175 bits)

NFT1_DROME / O76464 Nitrilase and fragile histidine triad fusion protein NitFhit; NFT-1 protein; EC 3.6.1.29; EC 3.5.-.- from Drosophila melanogaster (Fruit fly) (see paper)
    37% identity, 94% coverage of query (163 bits)

NIT2_HUMAN / Q9NQR4 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Homo sapiens (Human) (see 4 papers)
Q9NQR4 omega-amidase (EC 3.5.1.3) from Homo sapiens (see paper)
    35% identity, 97% coverage of query (159 bits)

4hg3A / P47016 Structural insights into yeast nit2: wild-type yeast nit2 in complex with alpha-ketoglutarate (see paper)
    33% identity, 96% coverage of query (157 bits)

NIT2_YEAST / P47016 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
P47016 deaminated glutathione amidase (EC 3.5.1.128) from Saccharomyces cerevisiae (see paper)
    33% identity, 96% coverage of query (157 bits)

NIT2_SCHPO / O94660 Probable hydrolase nit2; EC 3.5.-.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    39% identity, 94% coverage of query (157 bits)

NIT2_RAT / Q497B0 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Rattus norvegicus (Rat) (see 2 papers)
    34% identity, 97% coverage of query (157 bits)

4hg5A Structural insights into yeast nit2: wild-type yeast nit2 in complex with oxaloacetate
    33% identity, 96% coverage of query (157 bits)

Nit2 / Q9JHW2 ω-amidase NIT2 monomer (EC 3.5.1.3) from Mus musculus (see 2 papers)
NIT2_MOUSE / Q9JHW2 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Mus musculus (Mouse) (see 3 papers)
    34% identity, 97% coverage of query (154 bits)

4hgdA Structural insights into yeast nit2: c169s mutant of yeast nit2 in complex with an endogenous peptide-like ligand
    32% identity, 96% coverage of query (149 bits)

NIT3_YEAST / P49954 Omega-amidase NIT3; Nitrilase homolog 2; EC 3.5.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
    30% identity, 99% coverage of query (147 bits)

B8C1M9 nitrilase (EC 3.5.5.1) from Thalassiosira pseudonana (see paper)
    32% identity, 96% coverage of query (142 bits)

A0A0E3D8K7 nitrilase (EC 3.5.5.1); Aliphatic nitrilase (EC 3.5.5.7) from Fusarium proliferatum (see paper)
    32% identity, 97% coverage of query (134 bits)

B8A2V8 omega-amidase (EC 3.5.1.3) from Zea mays (see paper)
    32% identity, 96% coverage of query (127 bits)

B8A0E5 omega-amidase (EC 3.5.1.3) from Zea mays (see paper)
    31% identity, 97% coverage of query (127 bits)

NILP3_ARATH / Q8RUF8 Omega-amidase, chloroplastic; Nitrilase-like protein 3; EC 3.5.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q8RUF8 omega-amidase (EC 3.5.1.3) from Arabidopsis thaliana (see paper)
    30% identity, 94% coverage of query (121 bits)

YBEM_ECOBD / A0A140NCB4 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; ecYbeM; EC 3.5.1.128 from Escherichia coli (strain B / BL21-DE3) (see paper)
    32% identity, 93% coverage of query (119 bits)

ORF77 / Q93NG1 2-ketoglutaramate amidase monomer (EC 3.5.1.3) from Paenarthrobacter nicotinovorans (see 2 papers)
NIT_PAENI / Q93NG1 2-oxoglutaramate amidase; Omega-amidase; EC 3.5.1.111 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
Q93NG1 2-oxoglutaramate amidase (EC 3.5.1.111) from Paenarthrobacter nicotinovorans (see paper)
    32% identity, 85% coverage of query (107 bits)

Build an alignment

Build an alignment for WP_011384016.1 and 24 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

mtnU / O31664 2-oxoglutaramate:glutamine aminotransferase (EC 3.5.1.3) from Bacillus subtilis (strain 168) (see 4 papers)
MTNU_BACSU / O31664 2-oxoglutaramate amidase; EC 3.5.1.111 from Bacillus subtilis (strain 168) (see 2 papers)
    29% identity, 95% coverage of query (100 bits)

A0QQY9 D-inositol-3-phosphate glycosyltransferase (EC 2.4.1.250) from Mycolicibacterium smegmatis (see paper)
    30% identity, 96% coverage of query (92.4 bits)

3klcA / Q9UYV8 Crystal structure of hyperthermophilic nitrilase (see paper)
    31% identity, 92% coverage of query (90.1 bits)

At2g27450 / Q8VYF5 At2g27450-monomer (EC 3.5.1.53) from Arabidopsis thaliana (see paper)
NILP1_ARATH / Q8VYF5 N-carbamoylputrescine amidase; Nitrilase-like protein 1; EC 3.5.1.53 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    26% identity, 96% coverage of query (90.1 bits)

NITR_PYRAB / Q9UYV8 Nitrilase; PaNit; EC 3.5.5.1 from Pyrococcus abyssi (strain GE5 / Orsay) (see 2 papers)
    31% identity, 92% coverage of query (90.1 bits)

3klcB Crystal structure of hyperthermophilic nitrilase
    31% identity, 92% coverage of query (90.1 bits)

6ypaB / O58376 The c146a variant of an amidase from pyrococcus horikoshii with bound glutaramide
    30% identity, 82% coverage of query (85.1 bits)

7ovgA The c146a variant of an amidase from pyrococcus horikoshii with bound acetamide
    30% identity, 82% coverage of query (84.7 bits)

5h8iC / G7ITU5 Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) in complex with n-(dihydroxymethyl)putrescine (see paper)
    25% identity, 93% coverage of query (84.0 bits)

5h8jB Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) in complex with cadaverine
    25% identity, 93% coverage of query (84.0 bits)

AGUB_SOLLC / Q9XGI9 N-carbamoylputrescine amidase; EC 3.5.1.53 from Solanum lycopersicum (Tomato) (Lycopersicon esculentum) (see paper)
    27% identity, 96% coverage of query (83.2 bits)

D2SGH7 nitrilase (EC 3.5.5.1) from Geodermatophilus obscurus (see paper)
    29% identity, 90% coverage of query (82.8 bits)

Pf1N1B4_2504 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N1B4
    30% identity, 88% coverage of query (82.0 bits)

AO356_14225 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N2C3
    31% identity, 92% coverage of query (81.3 bits)

PfGW456L13_1170 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens GW456-L13
    30% identity, 92% coverage of query (80.9 bits)

5h8lB Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) c158s mutant in complex with putrescine
    24% identity, 93% coverage of query (80.5 bits)

Pf6N2E2_4777 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N2E2
    31% identity, 92% coverage of query (79.7 bits)

A0A088BHP3 aryl-acylamidase (EC 3.5.1.13); amidase (EC 3.5.1.4) from Streptomyces sp. (see paper)
    29% identity, 97% coverage of query (78.2 bits)

A3DHT2 nitrilase (EC 3.5.5.1) from Acetivibrio thermocellus (see paper)
    27% identity, 94% coverage of query (77.4 bits)

YAFV_STAA4 / P0DP65 Omega-amidase YafV; saYafV; EC 3.5.1.3 from Staphylococcus aureus (strain 04-02981) (see paper)
    28% identity, 72% coverage of query (76.3 bits)

AO353_07425 5-aminopentanamidase (EC 3.5.1.30) from Pseudomonas fluorescens FW300-N2E3
    29% identity, 88% coverage of query (74.7 bits)

D7B8P3 nitrilase (EC 3.5.5.1) from Nocardiopsis dassonvillei ATCC 23218 (see paper)
    31% identity, 72% coverage of query (74.7 bits)

aguB / Q9I6J8 N-carbamoylputrascine amidohydrolase subunit (EC 3.5.1.53) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    29% identity, 86% coverage of query (73.9 bits)

ctu / Q5NHL7 citrullinase (EC 3.5.1.20) from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) (see paper)
CTU_FRATT / Q5NHL7 Citrullinase; Citrulline ureidase; CTU; EC 3.5.1.20 from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) (see paper)
Q5NHL7 citrullinase (EC 3.5.1.20) from Francisella tularensis subsp. tularensis (see paper)
    26% identity, 88% coverage of query (73.2 bits)

F8AEP0 amidase (EC 3.5.1.4) from Pyrococcus yayanosii (see paper)
    28% identity, 87% coverage of query (72.0 bits)

pydC / Q846U4 3-amino-isobutyrate synthase (EC 3.5.1.6) from Brevibacillus agri (see paper)
    28% identity, 72% coverage of query (70.9 bits)

YCU9_SCHPO / O59829 Probable nitrilase C965.09; EC 3.5.-.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    24% identity, 98% coverage of query (69.7 bits)

C6K3Z5 amidase (EC 3.5.1.4) from Nesterenkonia sp. (see paper)
    29% identity, 84% coverage of query (69.3 bits)

ramA / Q75SP7 (R)-amidase (EC 3.5.1.100) from Pseudomonas sp. (see paper)
RSAM_PSESP / Q75SP7 (R)-stereoselective amidase; EC 3.5.1.100 from Pseudomonas sp. (see paper)
Q75SP7 (R)-amidase (EC 3.5.1.100) from Pseudomonas sp. (see paper)
    25% identity, 96% coverage of query (69.3 bits)

4izuA The e41q mutant of the amidase from nesterenkonia sp. An1 showing the result of michael addition of acrylamide at the active site cysteine
    28% identity, 85% coverage of query (68.9 bits)

aguB / A0A1J6PBK3 N-carbamoylputrescine amidohydrolase (EC 3.5.1.53) from Campylobacter jejuni (see paper)
    25% identity, 96% coverage of query (67.0 bits)

4iztA / D0VWZ1 The e41q mutant of the amidase from nesterenkonia sp. An1 showing covalent addition of the acetamide moiety of fluoroacetamide at the active site cysteine
    28% identity, 85% coverage of query (67.0 bits)

Q5KR04 N-carbamoylputrescine amidase (EC 3.5.1.53) from Selenomonas ruminantium (see paper)
    27% identity, 91% coverage of query (66.6 bits)

5nycA A c145a mutant of nesterenkonia an1 amidase bound to propionitrile
    28% identity, 85% coverage of query (66.2 bits)

4izsA The c145a mutant of the amidase from nesterenkonia sp. An1 in complex with butyramide
    28% identity, 85% coverage of query (66.2 bits)

NIT1 / P32961 Nit1 (EC 3.5.5.1; EC 4.2.1.84) from Arabidopsis thaliana (see 5 papers)
NRL1_ARATH / P32961 Nitrilase 1; EC 3.5.5.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
P32961 deaminated glutathione amidase (EC 3.5.1.128); nitrilase (EC 3.5.5.1) from Arabidopsis thaliana (see 6 papers)
    26% identity, 93% coverage of query (65.9 bits)

YAFV_YEREN / P0DP67 Omega-amidase YafV; yeYafV; EC 3.5.1.3 from Yersinia enterocolitica (see paper)
    31% identity, 84% coverage of query (65.5 bits)

5nybA A c145a mutant of nesterenkonia an1 amidase bound to adipamide
    28% identity, 85% coverage of query (64.3 bits)

5ny7A A c145a mutant of nesterenkonia an1 amidase bound to nicotinamide
    28% identity, 85% coverage of query (64.3 bits)

NIT_RHIFH / G9AIU0 Aliphatic nitrilase; NitSf; EC 3.5.5.1 from Rhizobium fredii (strain HH103) (Sinorhizobium fredii) (see paper)
    24% identity, 97% coverage of query (60.8 bits)

NIT2 / P32962 Nit2 (EC 3.5.5.1; EC 4.2.1.84) from Arabidopsis thaliana (see 4 papers)
NRL2_ARATH / P32962 Nitrilase 2; EC 3.5.5.1 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
P32962 nitrilase (EC 3.5.5.1) from Arabidopsis thaliana (see paper)
    25% identity, 93% coverage of query (60.5 bits)

Q84FR7 N-carbamoyl-D-amino-acid hydrolase (EC 3.5.1.77) from Arthrobacter crystallopoietes (see paper)
    27% identity, 89% coverage of query (60.5 bits)

A0A023XI92 N-carbamoyl-D-amino-acid hydrolase (EC 3.5.1.77) from Bradyrhizobium japonicum (see paper)
    27% identity, 77% coverage of query (60.1 bits)

YHCX_BACSU / P54608 Probable hydrolase YhcX; EC 3.5.-.- from Bacillus subtilis (strain 168) (see paper)
    23% identity, 74% coverage of query (59.3 bits)

R0GJB9 nitrilase (EC 3.5.5.1) from Capsella rubella (see paper)
    25% identity, 92% coverage of query (59.3 bits)

A3QYW3 nitrilase (EC 3.5.5.1) from Brassica rapa (see paper)
    23% identity, 93% coverage of query (57.4 bits)

Q8PXI9 nitrilase (EC 3.5.5.1) from Methanosarcina mazei (see paper)
    26% identity, 96% coverage of query (57.0 bits)

R0H4W3 nitrilase (EC 3.5.5.1) from Capsella rubella (see paper)
    25% identity, 93% coverage of query (56.2 bits)

DCAS_ENSAD / Q5S260 N-carbamoyl-D-amino acid hydrolase; D-carbamoylase; EC 3.5.1.77 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
Q5S260 N-carbamoyl-D-amino-acid hydrolase (EC 3.5.1.77) from Ensifer adhaerens (see paper)
    26% identity, 76% coverage of query (53.9 bits)

Q44185 N-carbamoyl-D-amino acid hydrolase; D-N-alpha-carbamilase; EC 3.5.1.77 from Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)
    24% identity, 85% coverage of query (52.8 bits)

8hpcC / P60327 Crystal structure of c171a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-hydroxyphenylglycine
    24% identity, 88% coverage of query (52.0 bits)

1uf8A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-phenylalanine
    24% identity, 88% coverage of query (52.0 bits)

1uf7A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-valine
    24% identity, 88% coverage of query (52.0 bits)

1uf5A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-methionine
    24% identity, 88% coverage of query (52.0 bits)

Q1I7X1 nitrilase (EC 3.5.5.1) from Pseudomonas entomophila (see paper)
    25% identity, 95% coverage of query (48.5 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory