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Searching for up to 100 curated homologs for WP_011814428.1 NCBI__GCF_000015585.1:WP_011814428.1 (454 a.a.)

Found high-coverage hits (≥70%) to 78 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

TSBType2 / Q9FFW8 tryptophan synthase, beta subunit (EC 4.2.1.122) from Arabidopsis thaliana (see paper)
Q9FFW8 tryptophan synthase (EC 4.2.1.20) from Arabidopsis thaliana (see paper)
    58% identity, 97% coverage of query (535 bits)

trpB2 / Q9WZ09 tryptophan synthase β2 subunit (EC 4.2.1.122) from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
    55% identity, 92% coverage of query (486 bits)

Q5JDJ1 tryptophan synthase (indole-salvaging) (EC 4.2.1.122); tryptophan synthase (subunit 1/2) (EC 4.2.1.20) from Thermococcus kodakarensis (see paper)
    52% identity, 95% coverage of query (445 bits)

6hulB / P50383 Sulfolobus solfataricus tryptophan synthase ab complex (see paper)
    47% identity, 91% coverage of query (384 bits)

Q97TX6 tryptophan synthase (EC 4.2.1.20) from Saccharolobus solfataricus (see paper)
    43% identity, 94% coverage of query (338 bits)

4qysB / Q97TX6 Trpb2 enzymes (see paper)
    43% identity, 93% coverage of query (332 bits)

4qysA Trpb2 enzymes
    42% identity, 93% coverage of query (312 bits)

trpB / Q9YGB0 tryptophan synthase β subunit (EC 4.2.1.122) from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (see paper)
Q9YGB0 tryptophan synthase (indole-salvaging) (EC 4.2.1.122); tryptophan synthase (subunit 2/2) (EC 4.2.1.20) from Thermococcus kodakarensis (see paper)
    32% identity, 78% coverage of query (141 bits)

trpB / CAB55324.2 tryptophan synthase beta subunit from Rhizobium etli (see paper)
    33% identity, 76% coverage of query (133 bits)

5dw3A Tryptophan synthase beta-subunit from pyrococcus furiosus with product l-tryptophan non-covalently bound in the active site
    30% identity, 76% coverage of query (133 bits)

5vm5D Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9, with ser bound
    31% identity, 76% coverage of query (133 bits)

TRPB_MYCTU / P9WFX9 Tryptophan synthase beta chain; EC 4.2.1.20 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
    33% identity, 78% coverage of query (132 bits)

5tciH / P9WFX9 Crystal structure of tryptophan synthase from m. Tuberculosis - brd4592-bound form (see paper)
    33% identity, 78% coverage of query (132 bits)

6am8B Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9 with trp bound as e(aex2)
    30% identity, 76% coverage of query (132 bits)

6uapB Crystal structure of tryptophan synthase from m. Tuberculosis - open form with brd6309 bound
    33% identity, 78% coverage of query (132 bits)

6usaB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and gsk1-bound form
    32% identity, 77% coverage of query (132 bits)

6dweB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and brd0059-bound form
    32% identity, 77% coverage of query (132 bits)

6u6cB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and gsk2-bound form
    32% identity, 77% coverage of query (132 bits)

5ocwB Structure of mycobacterium tuberculosis tryptophan synthase in space group f222
    32% identity, 77% coverage of query (132 bits)

5ixjD / Q8U093 Tryptophan synthase beta-subunit from pyrococcus furiosus with l- threonine non-covalently bound in the active site (see paper)
    31% identity, 76% coverage of query (131 bits)

TRPB_RHIEC / Q2KE82 Tryptophan synthase beta chain; EC 4.2.1.20 from Rhizobium etli (strain ATCC 51251 / DSM 11541 / JCM 21823 / NBRC 15573 / CFN 42) (see paper)
    33% identity, 76% coverage of query (131 bits)

5t6mA Structure of the tryptophan synthase b-subunit from pyroccus furiosus with b-methyltryptophan non-covalently bound
    31% identity, 76% coverage of query (131 bits)

O28672 tryptophan synthase (EC 4.2.1.20) from Archaeoglobus fulgidus (see paper)
    30% identity, 77% coverage of query (130 bits)

Q8U093 tryptophan synthase (subunit 2/2) (EC 4.2.1.20) from Pyrococcus furiosus (see paper)
    31% identity, 76% coverage of query (130 bits)

7rnpA Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9_h275e with 4-cl-trp non-covalently bound
    30% identity, 76% coverage of query (130 bits)

5dw0A Trpb from pyrococcus furiosus with l-serine bound as the external aldimine
    31% identity, 76% coverage of query (130 bits)

5t6mB Structure of the tryptophan synthase b-subunit from pyroccus furiosus with b-methyltryptophan non-covalently bound
    30% identity, 76% coverage of query (130 bits)

1v8zA X-ray crystal structure of the tryptophan synthase b2 subunit from hyperthermophile, pyrococcus furiosus
    30% identity, 76% coverage of query (130 bits)

5ey5B Lbcats
    31% identity, 76% coverage of query (129 bits)

6cuzA Engineered trpb from pyrococcus furiosus, pftrpb7e6 with (2s,3r)- ethylserine bound as the amino-acrylate
    30% identity, 76% coverage of query (127 bits)

6cutA Engineered holo trpb from pyrococcus furiosus, pftrpb7e6 with (2s,3s)- isopropylserine bound as the external aldimine
    30% identity, 76% coverage of query (127 bits)

trpB1 / P50909 β subunit of tryptophan synthase (EC 4.2.1.122) from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see 2 papers)
P50909 tryptophan synthase (EC 4.2.1.20) from Thermotoga maritima (see paper)
    30% identity, 77% coverage of query (125 bits)

trpB / RF|YP_045379.1 tryptophan synthase, beta subunit; EC 4.2.1.20 from Acinetobacter calcoaceticus (see paper)
    30% identity, 83% coverage of query (125 bits)

Build an alignment

Build an alignment for WP_011814428.1 and 33 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

8vhhA / P50909 Engineered holo tryptophan synthase (tm9d8 ) Derived from t. Maritima trpb (see paper)
    29% identity, 77% coverage of query (120 bits)

8eh1A Engineered tyrosine synthase (tmtyrs1) derived from t. Maritima trpb with ser bound as the amino-acrylate intermediate and complexed with 4-hydroxyquinoline
    29% identity, 77% coverage of query (117 bits)

8eh0A Engineered tyrosine synthase (tmtyrs1) derived from t. Maritima trpb with ser bound as the amino-acrylate intermediate and complexed with quinoline n-oxide
    29% identity, 77% coverage of query (117 bits)

8egzA Engineered tyrosine synthase (tmtyrs1) derived from t. Maritima trpb with ser bound as the amino-acrylate intermediate
    29% identity, 77% coverage of query (117 bits)

trpB / GI|28971664 tryptophan synthase, beta subunit; EC 4.2.1.20 from Burkholderia multivorans ATCC 17616 (see paper)
trpB / BAC65264.1 tryptophan synthase beta chain from Burkholderia multivorans (see paper)
    30% identity, 76% coverage of query (113 bits)

trpB / AAD29261.1 tryptophan synthase beta chain from Rhodobacter sphaeroides (see paper)
    30% identity, 78% coverage of query (111 bits)

TRP_YEAST / P00931 Tryptophan synthase; EC 4.2.1.20 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
    29% identity, 77% coverage of query (111 bits)

TSB1 / AAA33490.1 tryptophan synthase beta-subunit, partial from Zea mays (see paper)
    30% identity, 77% coverage of query (110 bits)

Q60142 tryptophan synthase (EC 4.2.1.20) from Bacillus subtilis (see paper)
    30% identity, 77% coverage of query (109 bits)

TRP5 likely tryptophan synthetase alpha chain from Candida albicans (see paper)
    28% identity, 77% coverage of query (109 bits)

trpB bifunctional tryptophan synthase TRPB from Emericella nidulans (see 2 papers)
    29% identity, 77% coverage of query (108 bits)

TSB2 / AAA33491.1 tryptophan synthase beta-subunit from Zea mays (see paper)
    29% identity, 77% coverage of query (108 bits)

TSB1 / P14671 tryptophan synthase, beta subunit (EC 4.2.1.122) from Arabidopsis thaliana (see 3 papers)
P14671 tryptophan synthase (EC 4.2.1.20) from Arabidopsis thaliana (see paper)
    28% identity, 77% coverage of query (108 bits)

5kzmB / Q5NE79 Crystal structure of tryptophan synthase alpha-beta chain complex from francisella tularensis (see paper)
    29% identity, 77% coverage of query (102 bits)

A0A2U8JPY3 tryptophan synthase (indole-salvaging) (EC 4.2.1.122); tryptophan synthase (EC 4.2.1.20) from Psilocybe cubensis (see paper)
    28% identity, 77% coverage of query (102 bits)

6v82B / O84172 Crystal structure of tryptophan synthase from chlamydia trachomatis d/uw-3/cx (see paper)
    27% identity, 76% coverage of query (97.8 bits)

7jmqB / P0A2K1 The external aldimine form of the mutant beta-s377a salmonella thypi tryptophan synthase in open conformation showing dual side chain conformations for the residue beta-q114, sodium ion at the metal coordination site, and f9 inhibitor at the alpha-site. One of the beta-q114 rotamer conformations allows a hydrogen bond to form with the plp oxygen at the position 3 in the ring
    28% identity, 77% coverage of query (89.4 bits)

TRPB_SALTY / P0A2K1 Tryptophan synthase beta chain; EC 4.2.1.20 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
trpB / PDB|1KFJ_B tryptophan synthase beta chain; EC 4.2.1.20 from Salmonella enterica subsp. enterica serovar Typhimurium (see 10 papers)
    28% identity, 77% coverage of query (89.4 bits)

6xt0B Salmonella typhimurium tryptophan synthase complexed with dioxindolyl- l-alanine and d-glycerol-3-phosphate
    28% identity, 77% coverage of query (89.0 bits)

6xrhB Salmonella typhimurium tryptophan synthase complexed with oxindolyl-l- alanine and d-glycerol-3-phosphate
    28% identity, 77% coverage of query (89.0 bits)

6xoyB Salmonella typhimurium tryptophan synthase complexed with d-tryptophan and d-glycerol-3-phosphate
    28% identity, 77% coverage of query (89.0 bits)

6xinB The crystal structure of tryptophan synthase from salmonella enterica serovar typhimurium in complex with (2s)-3-amino-3-imino-2- phenyldiazenylpropanamide at the enzyme alpha-site.
    28% identity, 77% coverage of query (89.0 bits)

3pr2B Tryptophan synthase indoline quinonoid structure with f9 inhibitor in alpha site
    28% identity, 77% coverage of query (89.0 bits)

1beuB Trp synthase (d60n-ipp-ser) with k+
    28% identity, 77% coverage of query (89.0 bits)

6ducB Crystal structure of mutant beta-k167t tryptophan synthase in complex with inhibitor n-(4'-trifluoromethoxybenzenesulfonyl)-2-amino-1- ethylphosphate (f9f) at the alpha-site, cesium ion at the metal coordination site, and 2-aminophenol quinonoid (1d0) at the beta-site
    28% identity, 77% coverage of query (89.0 bits)

1a50B Crystal structure of wild-type tryptophan synthase complexed with 5- fluoroindole propanol phosphate
    28% identity, 77% coverage of query (89.0 bits)

5cgqB Crystal structure of tryptophan synthase from salmonella typhimurium in complex with f9 ligand in the alpha-site and the product l- tryptophan in the beta-site.
    28% identity, 77% coverage of query (89.0 bits)

1qopB Crystal structure of wild-type tryptophan synthase complexed with indole propanol phosphate
    28% identity, 77% coverage of query (89.0 bits)

7jqwB The external aldimine crystal structure of salmonella typhimurium tryptophan synthase mutant beta-s377a in complex with cesium ion at the metal coordination site. The single beta-q114 rotamer conformation allows a hydrogen bond to form with the plp oxygen at the position 3 in the ring
    28% identity, 77% coverage of query (89.0 bits)

7lv5B The internal aldimine form of the wild-type salmonella typhimurium tryptophan synthase in complex with inhibitor n-(4'- trifluoromethoxybenzenesulfonyl)-2-amino-1-ethylphosphate (f9f) at the enzyme alpha-site, cesium ion at the metal coordination site and l-histidine at the enzyme beta-site at 1.55 angstrom resolution
    28% identity, 77% coverage of query (89.0 bits)

3cepB Structure of a tryptophan synthase quinonoid intermediate
    28% identity, 77% coverage of query (89.0 bits)

2clfB Tryptophan synthase in complex with n-(4'- trifluoromethoxybenzoyl)-2-amino-1-ethylphosphate (f6) - highf6 complex
    28% identity, 77% coverage of query (89.0 bits)

8b05B Tryptophan synthase - cryo-trapping by the spitrobot crystal plunger after 20 sec
    28% identity, 77% coverage of query (88.6 bits)

2j9xB Tryptophan synthase in complex with gp, alpha-d,l-glycerol-phosphate, cs, ph6.5 - alpha aminoacrylate form - (gp)e(a-a)
    28% identity, 77% coverage of query (88.6 bits)

4kkxB Crystal structure of tryptophan synthase from salmonella typhimurium with 2-aminophenol quinonoid in the beta site and the f6 inhibitor in the alpha site
    28% identity, 77% coverage of query (88.6 bits)

4hpxB Crystal structure of tryptophan synthase at 1.65 a resolution in complex with alpha aminoacrylate e(a-a) and benzimidazole in the beta site and the f9 inhibitor in the alpha site
    28% identity, 77% coverage of query (88.6 bits)

4hpjB Crystal structure of tryptophan synthase at 1.45 a resolution in complex with 2-aminophenol quinonoid in the beta site and the f9 inhibitor in the alpha site
    28% identity, 77% coverage of query (88.6 bits)

4hn4B Tryptophan synthase in complex with alpha aminoacrylate e(a-a) form and the f9 inhibitor in the alpha site
    28% identity, 77% coverage of query (88.6 bits)

1a5sB Crystal structure of wild-type tryptophan synthase complexed with 5- fluoroindole propanol phosphate and l-ser bound as amino acrylate to the beta site
    28% identity, 77% coverage of query (87.4 bits)

2j9yB Tryptophan synthase q114n mutant in complex with compound ii
    28% identity, 77% coverage of query (86.7 bits)

2tysB Crystal structures of mutant (betak87t) tryptophan synthase alpha2 beta2 complex with ligands bound to the active sites of the alpha and beta subunits reveal ligand-induced conformational changes
    28% identity, 77% coverage of query (86.7 bits)

6o1hB Tryptophan synthase q114a mutant in complex with n-(4'- trifluoromethoxybenzenesulfonyl)-2-amino-1-ethylphosphate (f9f) at the enzyme alpha-site, cesium ion at the metal coordination site, and 2-aminophenol quinonoid at the enzyme beta site
    28% identity, 77% coverage of query (86.3 bits)

TrpB / b1261 tryptophan synthase subunit β (EC 4.2.1.122) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
trpB / P0A879 tryptophan synthase subunit β (EC 4.2.1.122) from Escherichia coli (strain K12) (see 7 papers)
    27% identity, 77% coverage of query (83.2 bits)

P0A879 Tryptophan synthase beta chain; EC 4.2.1.20 from Escherichia coli (strain K12)
    27% identity, 77% coverage of query (83.2 bits)

2rhgB / P0A2K1 Tryptophan synthase complexed with igp, ph 7.0, internal aldimine
    28% identity, 77% coverage of query (80.1 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory