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Searching for up to 100 curated homologs for WP_012067598.1 NCBI__GCF_000017145.1:WP_012067598.1 (258 a.a.)

Found high-coverage hits (≥70%) to 44 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

moeZ / P9WMN7 [sulfur-carrier protein CysO]--sulfur ligase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
MOEZ_MYCTU / P9WMN7 Probable adenylyltransferase/sulfurtransferase MoeZ; EC 2.7.7.-; EC 2.8.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
    56% identity, 96% coverage of query (257 bits)

ptmS4 / A0A2R3ZBG9 [sulfur-carrier protein]--sulfur ligase from Streptomyces platensis (see 3 papers)
    51% identity, 96% coverage of query (239 bits)

MOCS3_DROME / Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
    46% identity, 96% coverage of query (224 bits)

ttuC / B6ZH72 [sulfur carrier protein TtuB] adenylyltransferase from Thermus thermophilus (see paper)
    55% identity, 95% coverage of query (218 bits)

TTUC_THET2 / Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
    54% identity, 95% coverage of query (215 bits)

UBA4_EMENI / O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
cnxF adenylyltransferase and sulfurtransferase uba4; EC 2.7.7.-; EC 2.8.1.- from Emericella nidulans (see 2 papers)
    45% identity, 93% coverage of query (206 bits)

ubaA / D4GSF3 E1 SAMP-activating enzyme monomer (EC 2.7.7.100) from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (see 3 papers)
UBAA_HALVD / D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see 4 papers)
D4GSF3 SAMP-activating enzyme (EC 2.7.7.100); E1 SAMP-activating enzyme (EC 6.2.1.55) from Haloferax volcanii (see 8 papers)
    51% identity, 95% coverage of query (203 bits)

UBA4_YEAST / P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 8 papers)
    44% identity, 92% coverage of query (202 bits)

MOCS3 / O95396 MOCS3 adenylyltransferase/sulfurtransferase (EC 2.8.1.11; EC 2.7.7.80) from Homo sapiens (see 6 papers)
MOCS3_HUMAN / O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 6 papers)
O95396 molybdopterin-synthase adenylyltransferase (EC 2.7.7.80); molybdopterin synthase sulfurtransferase (EC 2.8.1.11) from Homo sapiens (see 6 papers)
    48% identity, 93% coverage of query (190 bits)

ChlN / b0826 molybdopterin-synthase adenylyltransferase (EC 2.7.7.80) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
moeB / P12282 molybdopterin-synthase adenylyltransferase (EC 2.7.7.80) from Escherichia coli (strain K12) (see 10 papers)
MOEB_ECOLI / P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
P12282 molybdopterin-synthase adenylyltransferase (EC 2.7.7.80) from Escherichia coli (see 2 papers)
moeB / GB|BAA35514.1 molybdopterin biosynthesis protein moeB from Escherichia coli K12 (see 4 papers)
    45% identity, 96% coverage of query (182 bits)

1jw9B / P12282 Structure of the native moeb-moad protein complex (see paper)
    44% identity, 96% coverage of query (176 bits)

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex
    44% identity, 96% coverage of query (176 bits)

1jwaB Structure of the atp-bound moeb-moad protein complex
    42% identity, 96% coverage of query (166 bits)

6yubA / G0SC54 Crystal structure of uba4 from chaetomium thermophilum (see paper)
    39% identity, 86% coverage of query (152 bits)

1zfnA / P30138 Structural analysis of escherichia coli thif (see paper)
    41% identity, 93% coverage of query (151 bits)

ThiA / b3992 sulfur carrier protein ThiS adenylyltransferase (EC 2.7.7.73) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
thiF / P30138 sulfur carrier protein ThiS adenylyltransferase (EC 2.7.7.73) from Escherichia coli (strain K12) (see 11 papers)
THIF_ECOLI / P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 4 papers)
P30138 sulfur carrier protein ThiS adenylyltransferase (EC 2.7.7.73) from Escherichia coli (see 3 papers)
    41% identity, 93% coverage of query (151 bits)

6yubB Crystal structure of uba4 from chaetomium thermophilum
    38% identity, 86% coverage of query (150 bits)

1zud3 Structure of this-thif protein complex
    40% identity, 93% coverage of query (147 bits)

thiF / O31619 [sulfur-carrier protein ThiS] adenylyltransferase (EC 2.7.7.73) from Bacillus subtilis (strain 168) (see 4 papers)
O31619 sulfur carrier protein ThiS adenylyltransferase (EC 2.7.7.73) from Bacillus subtilis (see paper)
    35% identity, 87% coverage of query (112 bits)

TC 3.A.25.2.1 / Q8ID54 UBA/THIF-type NAD/FAD binding protein, putative, component of The SELMA system in the apicoplast of the malaria paraiste, Plasmodium falciparum from Plasmodium falciparum (isolate 3D7)
    31% identity, 76% coverage of query (103 bits)

Build an alignment

Build an alignment for WP_012067598.1 and 20 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

3kydB / Q9UBT2 Human sumo e1~sumo1-amp tetrahedral intermediate mimic (see paper)
    28% identity, 79% coverage of query (79.0 bits)

TC 3.A.25.1.1 / B7FYG1 Ubiquitin-activating enzyme E1, protein 1, component of The Marine diatom SELMA pre-protein translocase complex from Phaeodactylum tricornutum (strain CCAP 1055/1)
    29% identity, 71% coverage of query (77.0 bits)

UBE12_ARATH / P92974 Ubiquitin-activating enzyme E1 2; AtUBA2; EC 6.2.1.45 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
P92974 E2 ubiquitin-conjugating enzyme (EC 2.3.2.23); E1 ubiquitin-activating enzyme (EC 6.2.1.45) from Arabidopsis thaliana (see 2 papers)
    32% identity, 72% coverage of query (72.4 bits)

UBA1_SCHPO / O94609 Ubiquitin-activating enzyme E1 1; Poly(A)+ RNA transport protein 3; EC 6.2.1.45 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
ptr3 ubiquitin activating enzyme E1 from Schizosaccharomyces pombe (see 2 papers)
    28% identity, 72% coverage of query (72.4 bits)

4ii3A / O94609 Crystal structure of s. Pombe ubiquitin activating enzyme 1 (uba1) in complex with ubiquitin and atp/mg (see paper)
    28% identity, 72% coverage of query (72.0 bits)

4ii3C Crystal structure of s. Pombe ubiquitin activating enzyme 1 (uba1) in complex with ubiquitin and atp/mg
    28% identity, 72% coverage of query (72.0 bits)

6o82A S. Pombe ubiquitin e1 complex with a ubiquitin-amp mimic
    28% identity, 72% coverage of query (72.0 bits)

6o83A S. Pombe ubiquitin e1~ubiquitin-amp tetrahedral intermediate mimic
    28% identity, 72% coverage of query (72.0 bits)

9b5hA Ubiquitin-activating enzyme E1 1
    28% identity, 72% coverage of query (72.0 bits)

9b5cA Ubiquitin-activating enzyme E1 1
    28% identity, 72% coverage of query (72.0 bits)

TC 3.A.16.1.2 / E7LWL7 Uba1p, component of ER retrotranslocon for misfolded luminal ER proteins from Saccharomyces cerevisiae (strain VIN 13)
    29% identity, 72% coverage of query (70.5 bits)

5l6iA / P22515 Uba1 in complex with ub-mln4924 covalent adduct (see paper)
    29% identity, 72% coverage of query (70.5 bits)

UBA1_YEAST / P22515 Ubiquitin-activating enzyme E1 1; EC 6.2.1.45 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 5 papers)
P22515 E1 ubiquitin-activating enzyme (EC 6.2.1.45) from Saccharomyces cerevisiae (see paper)
    29% identity, 72% coverage of query (70.5 bits)

6nyaA Crystal structure of ubiquitin e1 (uba1) in complex with ubc3 (cdc34) and ubiquitin
    29% identity, 72% coverage of query (70.5 bits)

5l6jA Uba1 in complex with ub-mln7243 covalent adduct
    29% identity, 72% coverage of query (70.5 bits)

5l6hA Uba1 in complex with ub-abpa3 covalent adduct
    29% identity, 72% coverage of query (70.5 bits)

4nnjA Crystal structure of uba1 in complex with ubiquitin-amp and thioesterified ubiquitin
    29% identity, 72% coverage of query (70.5 bits)

3cmmA Crystal structure of the uba1-ubiquitin complex
    29% identity, 72% coverage of query (70.5 bits)

5tr4A Structure of ubiquitin activating enzyme (uba1) in complex with ubiquitin and tak-243
    29% identity, 72% coverage of query (70.1 bits)

UBA2 / P31251 ubiquitin-activating enzyme (EC 6.2.1.45) from Triticum aestivum (see paper)
    31% identity, 72% coverage of query (67.8 bits)

UBA1 / P20973 ubiquitin-activating enzyme (EC 6.2.1.45) from Triticum aestivum (see paper)
UBE11_WHEAT / P20973 Ubiquitin-activating enzyme E1 1; EC 6.2.1.45 from Triticum aestivum (Wheat) (see paper)
    31% identity, 72% coverage of query (67.8 bits)

UBE11_ARATH / P93028 Ubiquitin-activating enzyme E1 1; AtUBA1; Protein MODIFIER OF SNC1 5; EC 6.2.1.45 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
P93028 E1 ubiquitin-activating enzyme (EC 6.2.1.45) from Arabidopsis thaliana (see 2 papers)
    32% identity, 72% coverage of query (67.0 bits)

UBA3 / P31252 ubiquitin-activating enzyme (EC 6.2.1.45) from Triticum aestivum (see paper)
UBE13_WHEAT / P31252 Ubiquitin-activating enzyme E1 3; EC 6.2.1.45 from Triticum aestivum (Wheat) (see paper)
    30% identity, 74% coverage of query (63.2 bits)

UBA1Y_MOUSE / P31254 Ubiquitin-like modifier-activating enzyme 1 Y; Ubiquitin-activating enzyme E1; Ubiquitin-activating enzyme E1 Y; EC 6.2.1.45 from Mus musculus (Mouse) (see paper)
    23% identity, 83% coverage of query (45.8 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory