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Searching for up to 100 curated homologs for WP_012465627.1 NCBI__GCF_000020465.1:WP_012465627.1 (253 a.a.)

Found high-coverage hits (≥70%) to 48 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

ptmS4 / A0A2R3ZBG9 [sulfur-carrier protein]--sulfur ligase from Streptomyces platensis (see 3 papers)
    42% identity, 99% coverage of query (196 bits)

ubaA / D4GSF3 E1 SAMP-activating enzyme monomer (EC 2.7.7.100) from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (see 3 papers)
UBAA_HALVD / D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see 4 papers)
D4GSF3 SAMP-activating enzyme (EC 2.7.7.100); E1 SAMP-activating enzyme (EC 6.2.1.55) from Haloferax volcanii (see 8 papers)
    42% identity, 99% coverage of query (178 bits)

moeZ / P9WMN7 [sulfur-carrier protein CysO]--sulfur ligase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
MOEZ_MYCTU / P9WMN7 Probable adenylyltransferase/sulfurtransferase MoeZ; EC 2.7.7.-; EC 2.8.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
    38% identity, 97% coverage of query (169 bits)

ttuC / B6ZH72 [sulfur carrier protein TtuB] adenylyltransferase from Thermus thermophilus (see paper)
    39% identity, 100% coverage of query (166 bits)

UBA4_EMENI / O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
cnxF adenylyltransferase and sulfurtransferase uba4; EC 2.7.7.-; EC 2.8.1.- from Emericella nidulans (see 2 papers)
    38% identity, 98% coverage of query (165 bits)

TTUC_THET2 / Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
    39% identity, 100% coverage of query (164 bits)

ChlN / b0826 molybdopterin-synthase adenylyltransferase (EC 2.7.7.80) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
moeB / P12282 molybdopterin-synthase adenylyltransferase (EC 2.7.7.80) from Escherichia coli (strain K12) (see 10 papers)
MOEB_ECOLI / P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
P12282 molybdopterin-synthase adenylyltransferase (EC 2.7.7.80) from Escherichia coli (see 2 papers)
moeB / GB|BAA35514.1 molybdopterin biosynthesis protein moeB from Escherichia coli K12 (see 4 papers)
    38% identity, 98% coverage of query (159 bits)

1jw9B / P12282 Structure of the native moeb-moad protein complex (see paper)
    37% identity, 98% coverage of query (155 bits)

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex
    37% identity, 98% coverage of query (155 bits)

MOCS3_DROME / Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
    38% identity, 99% coverage of query (153 bits)

6yubA / G0SC54 Crystal structure of uba4 from chaetomium thermophilum (see paper)
    36% identity, 85% coverage of query (142 bits)

6yubB Crystal structure of uba4 from chaetomium thermophilum
    36% identity, 85% coverage of query (142 bits)

1zud3 Structure of this-thif protein complex
    36% identity, 98% coverage of query (142 bits)

ThiA / b3992 sulfur carrier protein ThiS adenylyltransferase (EC 2.7.7.73) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
thiF / P30138 sulfur carrier protein ThiS adenylyltransferase (EC 2.7.7.73) from Escherichia coli (strain K12) (see 11 papers)
THIF_ECOLI / P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 4 papers)
P30138 sulfur carrier protein ThiS adenylyltransferase (EC 2.7.7.73) from Escherichia coli (see 3 papers)
    37% identity, 99% coverage of query (141 bits)

1zfnA / P30138 Structural analysis of escherichia coli thif (see paper)
    37% identity, 98% coverage of query (140 bits)

1jwaB Structure of the atp-bound moeb-moad protein complex
    36% identity, 94% coverage of query (134 bits)

UBA4_YEAST / P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 8 papers)
    37% identity, 85% coverage of query (131 bits)

MOCS3 / O95396 MOCS3 adenylyltransferase/sulfurtransferase (EC 2.8.1.11; EC 2.7.7.80) from Homo sapiens (see 6 papers)
MOCS3_HUMAN / O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 6 papers)
O95396 molybdopterin-synthase adenylyltransferase (EC 2.7.7.80); molybdopterin synthase sulfurtransferase (EC 2.8.1.11) from Homo sapiens (see 6 papers)
    36% identity, 99% coverage of query (129 bits)

thiF / O31619 [sulfur-carrier protein ThiS] adenylyltransferase (EC 2.7.7.73) from Bacillus subtilis (strain 168) (see 4 papers)
O31619 sulfur carrier protein ThiS adenylyltransferase (EC 2.7.7.73) from Bacillus subtilis (see paper)
    31% identity, 96% coverage of query (115 bits)

TC 3.A.25.2.1 / Q8ID54 UBA/THIF-type NAD/FAD binding protein, putative, component of The SELMA system in the apicoplast of the malaria paraiste, Plasmodium falciparum from Plasmodium falciparum (isolate 3D7)
    32% identity, 95% coverage of query (105 bits)

Build an alignment

Build an alignment for WP_012465627.1 and 20 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca
    26% identity, 98% coverage of query (82.0 bits)

3h5nA Crystal structure of e. Coli mccb + atp
    26% identity, 98% coverage of query (82.0 bits)

3h5rA Crystal structure of e. Coli mccb + succinimide
    25% identity, 98% coverage of query (80.1 bits)

mccB / Q47506 MccB protein monomer from Escherichia coli (see paper)
    25% identity, 98% coverage of query (79.0 bits)

3h5aC / Q47506 Crystal structure of e. Coli mccb (see paper)
    25% identity, 98% coverage of query (79.0 bits)

UBA5_CAEEL / P91430 Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5 from Caenorhabditis elegans (see paper)
    24% identity, 78% coverage of query (75.1 bits)

SAE2_DANRE / Q7SXG4 SUMO-activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
    26% identity, 71% coverage of query (74.7 bits)

UBA5_DROME / Q9VYY3 Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; UFM1-activating enzyme from Drosophila melanogaster (Fruit fly) (see paper)
    29% identity, 89% coverage of query (72.4 bits)

TC 3.A.16.1.3 / Q8I553 Ubiquitin-activating enzyme, putative, component of ERAD system in the endoplasmic reticulum of the malaria parasite, Plasmodium falciparum from Plasmodium falciparum (isolate 3D7)
    31% identity, 73% coverage of query (70.5 bits)

6h77B / Q9GZZ9 E1 enzyme for ubiquitin like protein activation in complex with ubl (see paper)
    26% identity, 93% coverage of query (70.1 bits)

UBA5_HUMAN / Q9GZZ9 Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1 from Homo sapiens (Human) (see 21 papers)
Q9GZZ9 E1 ubiquitin-activating enzyme (EC 6.2.1.45) from Homo sapiens (see 3 papers)
    26% identity, 93% coverage of query (68.9 bits)

UBA5_MOUSE / Q8VE47 Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; UFM1-activating enzyme from Mus musculus (Mouse) (see 3 papers)
    24% identity, 78% coverage of query (63.5 bits)

UBA5_DANRE / X1WER2 Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; UFM1-activating enzyme from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
    25% identity, 81% coverage of query (63.5 bits)

UBA6_MOUSE / Q8C7R4 Ubiquitin-like modifier-activating enzyme 6; Ubiquitin-activating enzyme 6; Ubiquitin-activating enzyme E1-like protein 2; E1-L2; EC 6.2.1.45 from Mus musculus (Mouse) (see paper)
    27% identity, 75% coverage of query (62.8 bits)

5l95A Crystal structure of human uba5 in complex with ufm1 and amp
    26% identity, 87% coverage of query (61.6 bits)

3gucA Human ubiquitin-activating enzyme 5 in complex with amppnp
    26% identity, 87% coverage of query (60.1 bits)

7pvnB / A0AVT1 Crystal structure of human uba6 in complex with atp (see paper)
    27% identity, 75% coverage of query (58.9 bits)

7solC Crystal structures of the bispecific ubiquitin/fat10 activating enzyme, uba6
    27% identity, 75% coverage of query (58.9 bits)

7pvnA Crystal structure of human uba6 in complex with atp
    27% identity, 75% coverage of query (58.9 bits)

7solA / A0AVT1 Crystal structures of the bispecific ubiquitin/fat10 activating enzyme, uba6 (see paper)
    27% identity, 75% coverage of query (58.9 bits)

UBA6_HUMAN / A0AVT1 Ubiquitin-like modifier-activating enzyme 6; Ubiquitin-activating enzyme 6; Monocyte protein 4; MOP-4; Ubiquitin-activating enzyme E1-like protein 2; E1-L2; EC 6.2.1.45 from Homo sapiens (Human) (see 4 papers)
A0AVT1 E1 ubiquitin-activating enzyme (EC 6.2.1.45) from Homo sapiens (see 4 papers)
    27% identity, 75% coverage of query (58.2 bits)

5iaaB / Q9GZZ9 Crystal structure of human uba5 in complex with ufm1 (see paper)
    24% identity, 87% coverage of query (51.6 bits)

6o82A S. Pombe ubiquitin e1 complex with a ubiquitin-amp mimic
    22% identity, 84% coverage of query (47.0 bits)

4ii3A / O94609 Crystal structure of s. Pombe ubiquitin activating enzyme 1 (uba1) in complex with ubiquitin and atp/mg (see paper)
    22% identity, 84% coverage of query (47.0 bits)

4ii3C Crystal structure of s. Pombe ubiquitin activating enzyme 1 (uba1) in complex with ubiquitin and atp/mg
    22% identity, 84% coverage of query (47.0 bits)

9b5hA Ubiquitin-activating enzyme E1 1
    22% identity, 84% coverage of query (47.0 bits)

9b5cA Ubiquitin-activating enzyme E1 1
    22% identity, 84% coverage of query (47.0 bits)

UBA1_SCHPO / O94609 Ubiquitin-activating enzyme E1 1; Poly(A)+ RNA transport protein 3; EC 6.2.1.45 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
ptr3 ubiquitin activating enzyme E1 from Schizosaccharomyces pombe (see 2 papers)
    22% identity, 84% coverage of query (44.3 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory