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Searching for up to 100 curated homologs for WP_012674645.1 NCBI__GCF_000021545.1:WP_012674645.1 (533 a.a.)

Found high-coverage hits (≥70%) to 29 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

cimA / Q74C76 (R)-citramalate synthase (EC 2.3.3.21) from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) (see paper)
CIMA_GEOSL / Q74C76 (R)-citramalate synthase; Citramalate synthase; EC 2.3.3.21 from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) (see paper)
Q74C76 (R)-citramalate synthase (EC 2.3.3.21) from Geobacter sulfurreducens (see paper)
    57% identity, 97% coverage of query (621 bits)

DVU1914 (R)-citramalate synthase (EC 2.3.3.21) from Desulfovibrio vulgaris Hildenborough JW710
    51% identity, 98% coverage of query (545 bits)

B0K6M2 (R)-citramalate synthase (EC 2.3.3.21) from Thermoanaerobacter sp. (see paper)
    51% identity, 98% coverage of query (531 bits)

CIMA_SULAC / Q4J6H1 (R)-citramalate synthase; Citramalate synthase; CMS; EC 2.3.3.21 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
    47% identity, 97% coverage of query (467 bits)

cimA / Q58787 (R)-citratemalate synthase subunit (EC 2.3.3.21) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see paper)
CIMA_METJA / Q58787 (R)-citramalate synthase CimA; EC 2.3.3.21 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58787 (R)-citramalate synthase (EC 2.3.3.21) from Methanocaldococcus jannaschii (see 2 papers)
    33% identity, 96% coverage of query (242 bits)

LEU1_METJA / Q58595 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58595 2-isopropylmalate synthase (EC 2.3.3.13) from Methanocaldococcus jannaschii (see paper)
    34% identity, 96% coverage of query (237 bits)

Q72JC9 2-isopropylmalate synthase (EC 2.3.3.13) from Thermus thermophilus (see 2 papers)
    31% identity, 96% coverage of query (212 bits)

LEU1_SULAC / Q4JA78 2-isopropylmalate synthase; IPMS; Alpha-isopropylmalate synthase; Alpha-IPM synthase; EC 2.3.3.13 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
    35% identity, 71% coverage of query (204 bits)

Build an alignment

Build an alignment for WP_012674645.1 and 8 homologs with ≥ 30% identity

Select sequences

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Change minimum %identity:

Additional hits (identity < 30%)

LEU1_SALTY / P15875 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 3 papers)
    29% identity, 96% coverage of query (195 bits)

LeuA / b0074 2-isopropylmalate synthase (EC 2.3.3.13) from Escherichia coli K-12 substr. MG1655 (see 7 papers)
leuA / P09151 2-isopropylmalate synthase (EC 2.3.3.13) from Escherichia coli (strain K12) (see 15 papers)
    30% identity, 96% coverage of query (192 bits)

6ktqA / Q4J989 Crystal structure of catalytic domain of homocitrate synthase from sulfolobus acidocaldarius (sahcs(dram)) in complex with alpha- ketoglutarate/zn2+/coa (see paper)
    31% identity, 70% coverage of query (179 bits)

HOSA_SULAC / Q4J989 Homocitrate synthase; HCS; EC 2.3.3.14 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
Q4J989 homocitrate synthase (EC 2.3.3.14) from Sulfolobus acidocaldarius (see paper)
    31% identity, 70% coverage of query (178 bits)

BT1861 2-isopropylmalate synthase (EC 2.3.3.13) from Bacteroides thetaiotaomicron VPI-5482
    29% identity, 97% coverage of query (172 bits)

LEU1_NEIMB / Q9JZG1 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Neisseria meningitidis serogroup B (strain MC58) (see 2 papers)
Q9JZG1 2-isopropylmalate synthase (EC 2.3.3.13) from Neisseria meningitidis (see 2 papers)
    29% identity, 83% coverage of query (172 bits)

LEU11_LEPIN / Q8F445 2-isopropylmalate synthase 1; Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1; EC 2.3.3.13 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see paper)
    28% identity, 96% coverage of query (161 bits)

P58637 homocitrate synthase (EC 2.3.3.14) from Nostoc sp. PCC 7120 = FACHB-418 (see paper)
    32% identity, 70% coverage of query (160 bits)

BT1858 (R)-citramalate synthase (EC 2.3.1.182) from Bacteroides thetaiotaomicron VPI-5482
    28% identity, 95% coverage of query (147 bits)

LEU11_ARATH / Q9LPR4 2-isopropylmalate synthase 1, chloroplastic; Methylthioalkylmalate synthase-like 4; EC 2.3.3.13 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
Q9LPR4 2-isopropylmalate synthase (EC 2.3.3.13) from Arabidopsis thaliana (see 3 papers)
    27% identity, 91% coverage of query (146 bits)

Q2QXY9 2-isopropylmalate synthase (EC 2.3.3.13) from Oryza sativa Japonica Group (see paper)
    27% identity, 96% coverage of query (145 bits)

Q2RAP8 2-isopropylmalate synthase (EC 2.3.3.13) from Oryza sativa Japonica Group (see paper)
    27% identity, 96% coverage of query (143 bits)

K4C627 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum lycopersicum (see paper)
    27% identity, 91% coverage of query (143 bits)

LEU12_ARATH / Q9C550 2-isopropylmalate synthase 2, chloroplastic; 2-isopropylmalate synthase 1; Methylthioalkylmalate synthase-like 3; EC 2.3.3.13 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9C550 2-isopropylmalate synthase (EC 2.3.3.13) from Arabidopsis thaliana (see 2 papers)
    28% identity, 90% coverage of query (143 bits)

LEU1_SOYBN / Q39891 Probable 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; Late nodulin-56; N-56; EC 2.3.3.13 from Glycine max (Soybean) (Glycine hispida) (see paper)
    26% identity, 90% coverage of query (142 bits)

Q30DX9 2-isopropylmalate synthase (EC 2.3.3.13) from Brassica insularis (see paper)
    27% identity, 90% coverage of query (140 bits)

D0VY45 homocitrate synthase (EC 2.3.3.14) from Lotus japonicus (see paper)
    24% identity, 94% coverage of query (125 bits)

cimA / Q8F3Q1 (R)-citramalate synthase subunit (EC 2.3.3.21) from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see paper)
CIMA_LEPIN / Q8F3Q1 (R)-citramalate synthase CimA; LiCMS; EC 2.3.3.21 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see 3 papers)
    26% identity, 92% coverage of query (111 bits)

CIRSY_SYNAS / Q2LTE1 Citrate (Re)-synthase; Re-citrate synthase; EC 2.3.3.3 from Syntrophus aciditrophicus (strain SB) (see paper)
    25% identity, 75% coverage of query (97.8 bits)

hphA / B2J8P8 2-benzylmalate synthase from Nostoc punctiforme (strain ATCC 29133 / PCC 73102) (see paper)
    25% identity, 71% coverage of query (89.7 bits)

pmmS / Q9LCB4 2-phosphinomethylmalate synthase monomer (EC 2.3.3.18) from Streptomyces hygroscopicus (see paper)
PMMS_STRHY / Q9LCB4 2-phosphinomethylmalate synthase; PMS; PMM synthase; EC 2.3.3.18 from Streptomyces hygroscopicus (see 2 papers)
Q9LCB4 2-phosphinomethylmalate synthase (EC 2.3.3.18); 2-phosphonomethylmalate synthase (EC 2.3.3.19) from Streptomyces hygroscopicus (see 4 papers)
    24% identity, 74% coverage of query (84.0 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory