Sites on a Tree

 

Searching for up to 100 curated homologs for WP_012976812.1 NCBI__GCF_000010725.1:WP_012976812.1 (343 a.a.)

Found high-coverage hits (≥70%) to 64 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

ASPG2_BACSU / O34482 L-asparaginase 2; L-ASNase 2; L-asparagine amidohydrolase 2; EC 3.5.1.1 from Bacillus subtilis (strain 168) (see paper)
    61% identity, 96% coverage of query (382 bits)

ASPG_DICCH / P06608 L-asparaginase; L-ASNase; L-asparagine amidohydrolase; EC 3.5.1.1 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see 3 papers)
    57% identity, 98% coverage of query (374 bits)

1hg0A X-ray structure of the complex between erwinia chrysanthemi l- asparaginase and succinic acid
    57% identity, 97% coverage of query (372 bits)

1jsrA Crystal structure of erwinia chrysanthemi l-asparaginase complexed with 6-hydroxy-l-norleucine
    57% identity, 96% coverage of query (371 bits)

1jslA Crystal structure of erwinia chrysanthemi l-asparaginase complexed with 6-hydroxy-d-norleucine
    57% identity, 96% coverage of query (371 bits)

7u6mC / P06608 Albumin binding domain fused to a mutant of the erwinia asparaginase (see paper)
    56% identity, 97% coverage of query (369 bits)

5k3oA / P50286 Wolinella succinogenes l-asparaginase p121 and l-aspartic acid (see paper)
    54% identity, 96% coverage of query (365 bits)

5k45A Wolinella succinogenes l-asparaginase p121 + l-glutamic acid
    54% identity, 96% coverage of query (365 bits)

ansB1 / Q6Q4F4 L-asparaginase subunit (EC 3.5.1.38) from Pectobacterium atrosepticum (see 10 papers)
Q6Q4F4 asparaginase (EC 3.5.1.1) from Pectobacterium atrosepticum (see paper)
    54% identity, 98% coverage of query (364 bits)

ASPQ_PSEFA / O68897 Glutaminase-asparaginase; L-ASNase/L-GLNase; L-asparagine/L-glutamine amidohydrolase; EC 3.5.1.38 from Pseudomonas fluorescens biotype A (see paper)
    58% identity, 96% coverage of query (363 bits)

2gvnA / I1SBD9 L-asparaginase from erwinia carotovora in complex with aspartic acid (see paper)
    55% identity, 96% coverage of query (363 bits)

P50286 asparaginase (EC 3.5.1.1) from Wolinella succinogenes (see paper)
    53% identity, 96% coverage of query (362 bits)

Q6Q4F3 asparaginase (EC 3.5.1.1) from Dickeya chrysanthemi (see 2 papers)
    55% identity, 98% coverage of query (358 bits)

6wywA Crystal structure of pseudomonas 7a glutaminase-asparaginase in complex with l-asp at ph 4.5
    55% identity, 96% coverage of query (351 bits)

6wyyA Crystal structure of pseudomonas 7a glutaminase-asparaginase in complex with l-glu at ph 6.5
    54% identity, 96% coverage of query (345 bits)

1hg1A X-ray structure of the complex between erwinia chrysanthemi l- asparaginase and d-aspartate
    54% identity, 96% coverage of query (343 bits)

1hfwA X-ray structure of the complex between erwinia chrysanthemi l- asparaginase and l-glutamate
    54% identity, 96% coverage of query (343 bits)

fraE / Q8ZLD3 N-(1-deoxy-D-fructos-1-yl)-L-asparaginase (EC 3.5.1.1) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
    52% identity, 98% coverage of query (341 bits)

1djoA / P10182 Crystal structure of pseudomonas 7a glutaminase-asparaginase with the inhibitor donv covalently bound in the active site (see paper)
    56% identity, 96% coverage of query (340 bits)

ansB / PDB|1AGX_A glutaminase-asparaginase; EC 3.5.1.38 from Acinetobacter glutaminasificans (see 3 papers)
    53% identity, 95% coverage of query (340 bits)

4pgaA Glutaminase-asparaginase from pseudomonas 7a
    56% identity, 96% coverage of query (340 bits)

1djpA Crystal structure of pseudomonas 7a glutaminase-asparaginase with the inhibitor don covalently bound in the active site
    56% identity, 96% coverage of query (340 bits)

6wyzA Crystal structure of pseudomonas 7a glutaminase-asparaginase (mutant k173m) in complex with d-glu at ph 5.5
    53% identity, 96% coverage of query (340 bits)

2hlnA L-asparaginase from erwinia carotovora in complex with glutamic acid
    53% identity, 96% coverage of query (339 bits)

ASPQ_PSES7 / P10182 Glutaminase-asparaginase; L-ASNase/L-GLNase; L-asparagine/L-glutamine amidohydrolase; PGA; EC 3.5.1.38 from Pseudomonas sp. (strain ATCC 29598 / 7A) (see paper)
    55% identity, 96% coverage of query (337 bits)

BPHYT_RS08815 L-asparaginase (EC 3.5.1.1) from Burkholderia phytofirmans PsJN
    50% identity, 100% coverage of query (336 bits)

C0KWF5 asparaginase (EC 3.5.1.1) from Escherichia coli (see paper)
    55% identity, 96% coverage of query (329 bits)

AnsB / b2957 L-asparaginase 2 (EC 3.5.1.38; EC 3.5.1.1) from Escherichia coli K-12 substr. MG1655 (see 4 papers)
ansB / P00805 L-asparaginase 2 (EC 3.5.1.38) from Escherichia coli (strain K12) (see 73 papers)
ASPG2_ECOLI / P00805 L-asparaginase 2; L-asparaginase II; L-ASNase II; L-asparagine amidohydrolase II; Colaspase; EC 3.5.1.1 from Escherichia coli (strain K12) (see 5 papers)
    55% identity, 96% coverage of query (327 bits)

1ho3A Crystal structure analysis of e. Coli l-asparaginase ii (y25f mutant)
    54% identity, 96% coverage of query (326 bits)

1jazA Crystal structure of monoclinic form of d90e mutant of escherichia coli asparaginase ii
    52% identity, 96% coverage of query (322 bits)

7r5qA Escherichia coli type ii asparaginase n24s mutant in complex with glu
    52% identity, 96% coverage of query (322 bits)

Q66CJ2 asparaginase (EC 3.5.1.1) from Yersinia pseudotuberculosis (see 2 papers)
    52% identity, 96% coverage of query (317 bits)

2wltA / Q9ZLB9 The crystal structure of helicobacter pylori l-asparaginase at 1.4 a resolution (see paper)
    49% identity, 96% coverage of query (315 bits)

Q9ZLB9 asparaginase (EC 3.5.1.1) from Helicobacter pylori J99 (see paper)
    48% identity, 96% coverage of query (311 bits)

6pa3A / P00805 E. Coli l-asparaginase ii double mutant (t89v,k162t) in complex with l-asn at ph 7.0 (see paper)
    54% identity, 96% coverage of query (308 bits)

B6ZCD8 asparaginase (EC 3.5.1.1) from Helicobacter pylori (see paper)
I0ZIE0 asparaginase (EC 3.5.1.1) from Helicobacter pylori (see paper)
    49% identity, 96% coverage of query (305 bits)

A0A2X5A091 asparaginase (EC 3.5.1.1); glutaminase (EC 3.5.1.2) from Helicobacter pylori (see paper)
    48% identity, 96% coverage of query (301 bits)

ASP22_YEAST / P0CX77 L-asparaginase 2-2; L-asparaginase II; L-asparagine amidohydrolase II; ASP II; EC 3.5.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
ASP23_YEAST / P0CX78 L-asparaginase 2-3; L-asparaginase II; L-asparagine amidohydrolase II; ASP II; EC 3.5.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
ASP24_YEAST / P0CX79 L-asparaginase 2-4; L-asparaginase II; L-asparagine amidohydrolase II; ASP II; EC 3.5.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
ASP21_YEAST / P0CZ17 L-asparaginase 2-1; L-asparaginase II; L-asparagine amidohydrolase II; ASP II; EC 3.5.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
P0CZ17 asparaginase (EC 3.5.1.1) from Saccharomyces cerevisiae (see paper)
    43% identity, 96% coverage of query (243 bits)

P38986 asparaginase (EC 3.5.1.1) from Saccharomyces cerevisiae (see paper)
    38% identity, 95% coverage of query (201 bits)

P38986 L-asparaginase 1; L-asparaginase I; L-asparagine amidohydrolase I; ASP I; EC 3.5.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    38% identity, 95% coverage of query (201 bits)

J9H7D1 asparaginase (EC 3.5.1.1) from Staphylococcus sp. (see paper)
    32% identity, 94% coverage of query (154 bits)

8h4aB / A0A6I7U6Y2 Blasnase-t13a/m57p
    31% identity, 91% coverage of query (140 bits)

7c8xA Blasnase-t13a with l-asn
    32% identity, 91% coverage of query (140 bits)

1zq1A / Q9V0T9 Structure of gatde tRNA-dependent amidotransferase from pyrococcus abyssi (see paper)
    34% identity, 96% coverage of query (139 bits)

7c8qA Blasnase-t13a with d-asn
    31% identity, 91% coverage of query (139 bits)

7cbuA Blasnase-t13a with l-asp
    33% identity, 76% coverage of query (138 bits)

Build an alignment

Build an alignment for WP_012976812.1 and 46 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

gatD / O26802 glutamyl-tRNA(Gln) amidotransferase subunit D (EC 6.3.5.7) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (see paper)
    29% identity, 97% coverage of query (122 bits)

ASPG_MYCTU / P9WPX5 L-asparaginase; L-ASNase; L-asparagine amidohydrolase; EC 3.5.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
    32% identity, 76% coverage of query (106 bits)

ASPG_THEKO / Q5JIW4 L-asparaginase; L-asparagine amidohydrolase; TkA; TkAsn; EC 3.5.1.1 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see 3 papers)
5ot0A / Q5JIW4 The thermostable l-asparaginase from thermococcus kodakarensis (see paper)
    28% identity, 94% coverage of query (81.6 bits)

ASPG1_BACSU / P26900 L-asparaginase 1; L-ASNase 1; L-asparagine amidohydrolase 1; EC 3.5.1.1 from Bacillus subtilis (strain 168) (see paper)
    25% identity, 96% coverage of query (79.0 bits)

CA265_RS25090 L-asparaginase (EC 3.5.1.1) from Pedobacter sp. GW460-11-11-14-LB5
    28% identity, 82% coverage of query (74.7 bits)

A0JNU3 1-alkyl-2-acetylglycerophosphocholine esterase (subunit 3/3) (EC 3.1.1.47) from Mus musculus (see paper)
    28% identity, 72% coverage of query (73.9 bits)

AnsA / b1767 L-asparaginase 1 (EC 3.5.1.38; EC 3.5.1.1) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
ansA / P0A962 L-asparaginase 1 (EC 3.5.1.38) from Escherichia coli (strain K12) (see 11 papers)
ASPG1_ECOLI / P0A962 L-asparaginase 1; L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I; EC 3.5.1.1 from Escherichia coli (strain K12) (see paper)
    25% identity, 85% coverage of query (72.8 bits)

ASPG_PYRFU / Q8TZE8 L-asparaginase; EC 3.5.1.1 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see 6 papers)
Q8TZE8 asparaginase (EC 3.5.1.1) from Pyrococcus furiosus (see 2 papers)
    24% identity, 73% coverage of query (70.9 bits)

4q0mA / Q8TZE8 Crystal structure of pyrococcus furiosus l-asparaginase (see paper)
    24% identity, 73% coverage of query (70.9 bits)

6nxcB / P0A962 Ecai(t162a) mutant in complex with citrate at ph 4 (see paper)
    24% identity, 78% coverage of query (67.8 bits)

2himA Crystal structure and allosteric regulation of the cytoplasmic escherichia coli l-asparaginase i
    24% identity, 75% coverage of query (65.9 bits)

7r6bB / A0A3N4B0Q2 Crystal structure of mutant r43d/l124d/r125a/c273s of l-asparaginase i from yersinia pestis (see paper)
    25% identity, 87% coverage of query (62.0 bits)

Pf6N2E2_4087 L-asparaginase (EC 3.5.1.1) from Pseudomonas fluorescens FW300-N2E2
    24% identity, 86% coverage of query (59.7 bits)

Pf1N1B4_2023 L-asparaginase (EC 3.5.1.1) from Pseudomonas fluorescens FW300-N1B4
    24% identity, 81% coverage of query (57.8 bits)

PfGW456L13_740 L-asparaginase (EC 3.5.1.1) from Pseudomonas fluorescens GW456-L13
    22% identity, 81% coverage of query (54.3 bits)

AO356_11100 L-asparaginase (EC 3.5.1.1) from Pseudomonas fluorescens FW300-N2C3
    23% identity, 81% coverage of query (54.3 bits)

AO353_09960 L-asparaginase (EC 3.5.1.1) from Pseudomonas fluorescens FW300-N2E3
    24% identity, 81% coverage of query (49.7 bits)

A0A1I0U0N3 asparaginase (EC 3.5.1.1) from Pseudomonas otitidis (see paper)
    23% identity, 94% coverage of query (45.8 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory