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Searching for up to 100 curated homologs for WP_013136746.1 NCBI__GCF_000092245.1:WP_013136746.1 (330 a.a.)

Found high-coverage hits (≥70%) to 40 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

PSEB_CAMJE / Q0P8W4 UDP-N-acetylglucosamine 4,6-dehydratase (inverting); Pseudaminic acid biosynthesis protein B; UDP-GlcNAc-inverting 4,6-dehydratase; EC 4.2.1.115 from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) (see paper)
    71% identity, 99% coverage of query (486 bits)

PSEB_CAMJJ / Q5QKR8 UDP-N-acetylglucosamine 4,6-dehydratase (inverting); Pseudaminic acid biosynthesis protein B; UDP-GlcNAc-inverting 4,6-dehydratase; EC 4.2.1.115 from Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) (see 2 papers)
    70% identity, 99% coverage of query (484 bits)

Q6VYQ6 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Helicobacter pylori (see paper)
    63% identity, 99% coverage of query (439 bits)

Q6VYQ5 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Helicobacter pylori (see paper)
    63% identity, 99% coverage of query (437 bits)

2gn4A / O25511 Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADPH and udp-glcnac (see paper)
    63% identity, 99% coverage of query (436 bits)

pseB / O25511 UDP-N-acetylglucosamine 4,6-dehydratase subunit (EC 4.2.1.115) from Helicobacter pylori (strain ATCC 700392 / 26695) (see 4 papers)
PSEB_HELPY / O25511 UDP-N-acetylglucosamine 4,6-dehydratase (inverting); Pseudaminic acid biosynthesis protein B; UDP-GlcNAc-inverting 4,6-dehydratase; EC 4.2.1.115 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see 3 papers)
    63% identity, 99% coverage of query (436 bits)

2gn8A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp
    63% identity, 99% coverage of query (436 bits)

2gnaA Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-gal
    63% identity, 99% coverage of query (436 bits)

2gn9A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-glc
    63% identity, 99% coverage of query (436 bits)

2gn6A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-glcnac
    63% identity, 99% coverage of query (436 bits)

BC_3750 / Q81A42 UDP-N-acetylglucosamine 4,6-dehydratase (EC 4.2.1.135) from Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711) (see 5 papers)
Q81A42 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (EC 4.2.1.135) from Bacillus cereus (see 2 papers)
    46% identity, 88% coverage of query (255 bits)

A0A0H3JPH0 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Staphylococcus aureus (see paper)
    44% identity, 94% coverage of query (239 bits)

6bwcC X-ray structure of pen from bacillus thuringiensis (see paper)
    41% identity, 86% coverage of query (238 bits)

3w1vA / A0A0H3JPH0 Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with inihibitor (see paper)
    43% identity, 94% coverage of query (236 bits)

4g5hA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with by-product
    43% identity, 94% coverage of query (236 bits)

wbvB / Q8L348 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Vibrio cholerae O37 (see 3 papers)
    40% identity, 100% coverage of query (233 bits)

4j2oC / P37362 Crystal structure of NADP-bound wbjb from a. Baumannii community strain d1279779 (see paper)
    45% identity, 85% coverage of query (231 bits)

3vvcA Crystal structure of capsular polysaccharide synthesizing enzyme cape , k126e, in apo form
    43% identity, 87% coverage of query (228 bits)

V5RBP5 UDP-glucose 4-epimerase (EC 5.1.3.2) from Acinetobacter baumannii (see paper)
    45% identity, 84% coverage of query (226 bits)

wbjB / Q9KID0 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Pseudomonas aeruginosa (see 2 papers)
    40% identity, 97% coverage of query (222 bits)

CAPD_RICPR / Q9ZDJ5 UDP-glucose 4-epimerase; Galactowaldenase; UDP-galactose 4-epimerase; EC 5.1.3.2 from Rickettsia prowazekii (strain Madrid E) (see paper)
    46% identity, 81% coverage of query (221 bits)

3vvbA / A0A0H3JPH0 Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in apo form (see paper)
    43% identity, 73% coverage of query (187 bits)

G5CSR9 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Megavirus chiliensis (see paper)
    38% identity, 85% coverage of query (180 bits)

4tqgA / G5CSR9 Crystal structure of megavirus udp-glcnac 4,6-dehydratase, 5-epimerase mg534 (see paper)
    36% identity, 85% coverage of query (166 bits)

cpsE polysaccharide capsule synthesis protein CpsE from Streptococcus iniae (see paper)
    39% identity, 80% coverage of query (161 bits)

EPSC_BACSU / P71052 Probable polysaccharide biosynthesis protein EpsC from Bacillus subtilis (strain 168) (see paper)
    35% identity, 80% coverage of query (160 bits)

wbiI / GI|3135688 putative epimerase/dehydratase WbiI from Burkholderia pseudomallei (see paper)
    33% identity, 81% coverage of query (152 bits)

wbpM / Q9KIC5 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (EC 4.2.1.135) from Pseudomonas aeruginosa (see 6 papers)
    32% identity, 82% coverage of query (150 bits)

wbgZ / Q9F736 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (EC 4.2.1.135) from Shigella sonnei (see paper)
    32% identity, 77% coverage of query (147 bits)

P72145 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Pseudomonas aeruginosa (see paper)
    32% identity, 82% coverage of query (147 bits)

RfbU / CAA69127.1 mannosyl-transferase from Vibrio cholerae (see paper)
    33% identity, 78% coverage of query (141 bits)

5bjuA / O86159 X-ray structure of the pglf dehydratase from campylobacter jejuni in complex with udp and NAD(h) (see paper)
    32% identity, 84% coverage of query (140 bits)

pglF / Q0P9D4 UDP-N-acetylglucosamine C-6 dehydratase (EC 4.2.1.135) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) (see 3 papers)
PGLF_CAMJE / Q0P9D4 UDP-N-acetyl-alpha-D-glucosamine C6 dehydratase; UDP-GlcNAc C6 dehydratase; Protein glycosylation pathway protein F; UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining); EC 4.2.1.135 from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) (see paper)
    32% identity, 84% coverage of query (140 bits)

5bjvA X-ray structure of the pglf udp-n-acetylglucosamine 4,6-dehydratase from campylobacterjejuni, d396n/k397a variant in complex with udp-n- acrtylglucosamine
    32% identity, 84% coverage of query (137 bits)

wbkD / Q2YMM0 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (EC 4.2.1.135) from Brucella abortus (strain 2308) (see 2 papers)
    31% identity, 77% coverage of query (135 bits)

Build an alignment

Build an alignment for WP_013136746.1 and 35 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

3pvzA / Q5E8L1 Udp-n-acetylglucosamine 4,6-dehydratase from vibrio fischeri
    29% identity, 78% coverage of query (86.3 bits)

pseB / A0A0A8J869 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Escherichia coli (see paper)
    26% identity, 89% coverage of query (82.4 bits)

6dntA / D3E402 Udp-n-acetylglucosamine 4-epimerase from methanobrevibacter ruminantium m1 in complex with udp-n-acetylmuramic acid (see paper)
    26% identity, 89% coverage of query (70.5 bits)

TGDS_HUMAN / O95455 dTDP-D-glucose 4,6-dehydratase; EC 4.2.1.46 from Homo sapiens (Human) (see paper)
    25% identity, 72% coverage of query (58.9 bits)

JF740056 / F8U969 UDP-glucose 4,6-dehydratase (EC 4.2.1.76) from Magnaporthe oryzae (see paper)
F8U969 UDP-glucose 4,6-dehydratase (EC 4.2.1.76) from Pyricularia grisea (see paper)
    24% identity, 74% coverage of query (55.5 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory