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Searching for up to 100 curated homologs for WP_013257171.1 NCBI__GCF_000143965.1:WP_013257171.1 (464 a.a.)

Found high-coverage hits (≥70%) to 57 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

LEUC1_SALTY / P15717 3-isopropylmalate dehydratase large subunit 1; Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1; EC 4.2.1.33 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
    62% identity, 100% coverage of query (595 bits)

LeuC / b0072 3-isopropylmalate dehydratase subunit LeuC (EC 4.2.1.33) from Escherichia coli K-12 substr. MG1655 (see 13 papers)
leuC / P0A6A6 3-isopropylmalate dehydratase subunit LeuC (EC 4.2.1.33) from Escherichia coli (strain K12) (see 11 papers)
leuC / ECOCYC|LEUC-MONOMER 3-isopropylmalate dehydratase large subunit; EC 4.2.1.33 from Escherichia coli K12 (see 9 papers)
    61% identity, 100% coverage of query (592 bits)

P9WQF5 3-isopropylmalate dehydratase (EC 4.2.1.33) from Mycobacterium tuberculosis (see paper)
    61% identity, 98% coverage of query (577 bits)

LEUC_MYCBO / Q7TXH6 3-isopropylmalate dehydratase large subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) (see paper)
    61% identity, 98% coverage of query (575 bits)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    58% identity, 100% coverage of query (570 bits)

leuC / Q8F4E6 isopropylmalate isomerase large subunit (EC 4.2.1.35) from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see paper)
    56% identity, 100% coverage of query (568 bits)

LEUC_YEAST / P07264 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
    57% identity, 100% coverage of query (560 bits)

AN5886 alpha isopropylmalate isomerase (Eurofung) from Emericella nidulans (see paper)
    57% identity, 99% coverage of query (559 bits)

leu1 / CAB99455.1 3-isopropylmalate dehydratase, partial from Yarrowia lipolytica (see paper)
    57% identity, 99% coverage of query (553 bits)

LEUC_CORGL / P58946 3-isopropylmalate dehydratase large subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
    54% identity, 98% coverage of query (524 bits)

hacA / Q58409 homoaconitase α subunit (EC 4.2.1.36) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see paper)
HACA_METJA / Q58409 Methanogen homoaconitase large subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
Q58409 methanogen homoaconitase (EC 4.2.1.114) from Methanocaldococcus jannaschii (see paper)
hacA homoaconitase; EC 4.2.1.-; EC 4.2.1.36 from Methanocaldococcus jannaschii (see 3 papers)
    34% identity, 94% coverage of query (230 bits)

DVU2982 3-isopropylmalate dehydratase / (R)-citramalate isomerase, large subunit LeuC (EC 4.2.1.33; EC 4.2.1.35) from Desulfovibrio vulgaris Hildenborough JW710
    35% identity, 100% coverage of query (224 bits)

4kp1A / P81291 Crystal structure of ipm isomerase large subunit from methanococcus jannaschii (mj0499) (see paper)
    32% identity, 96% coverage of query (222 bits)

leuC / P81291 isopropylmalate isomerase large subunit (EC 4.2.1.35) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see paper)
LEUC_METJA / P81291 Isopropylmalate/citramalate isomerase large subunit; (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease; EC 4.2.1.33; EC 4.2.1.35; EC 4.2.1.31 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
P81291 3-isopropylmalate dehydratase (EC 4.2.1.33); homoaconitate hydratase (EC 4.2.1.36) from Methanocaldococcus jannaschii (see paper)
    32% identity, 96% coverage of query (222 bits)

hphCD / B2J8P2 benzylmalate isomerase from Nostoc punctiforme (strain ATCC 29133 / PCC 73102) (see paper)
    32% identity, 96% coverage of query (216 bits)

4nqyA The reduced form of mj0499
    31% identity, 96% coverage of query (206 bits)

IPMI1 / Q94AR8 isopropylmalate isomerase large subunit (EC 4.2.1.33) from Arabidopsis thaliana (see paper)
LEUC_ARATH / Q94AR8 3-isopropylmalate dehydratase large subunit, chloroplastic; 2-(omega-methylthio)alkylmalate dehydratase large subunit; AtLEUC; Isopropylmalate isomerase large subunit 1; AtIIL1; IPMI LSU1; Methylthioalkylmalate isomerase large subunit; MAM-IL; EC 4.2.1.33; EC 4.2.1.170 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
Q94AR8 2-(omega-methylthio)alkylmalate dehydratase (subunit 2/2) (EC 4.2.1.170); 3-isopropylmalate dehydratase (EC 4.2.1.33) from Arabidopsis thaliana (see paper)
    33% identity, 96% coverage of query (205 bits)

lysT / Q9ZNE0 homoaconitase large subunit (EC 4.2.1.36) from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 3 papers)
HACA_THET2 / Q9ZNE0 Homoaconitase large subunit; HACN; Homoaconitate hydratase; EC 4.2.1.36 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
    37% identity, 88% coverage of query (204 bits)

dmdA / Q0QLE2 dimethylmaleate hydratase subunit A (EC 4.2.1.85) from Eubacterium barkeri (see paper)
DMDA_EUBBA / Q0QLE2 2,3-dimethylmalate dehydratase large subunit; EC 4.2.1.85 from Eubacterium barkeri (Clostridium barkeri) (see 2 papers)
    31% identity, 98% coverage of query (201 bits)

ACON2_YEAST / P39533 Homocitrate dehydratase, mitochondrial; Aconitase 2; EC 4.2.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 5 papers)
    30% identity, 86% coverage of query (153 bits)

Build an alignment

Build an alignment for WP_013257171.1 and 20 homologs with ≥ 30% identity

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Additional hits (identity < 30%)

LYS4_EMENI / Q92412 Homoaconitase, mitochondrial; Homoaconitate hydratase; EC 4.2.1.36 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
Q92412 homoaconitate hydratase (EC 4.2.1.36) from Aspergillus nidulans (see paper)
    28% identity, 93% coverage of query (150 bits)

lys2 / RF|NP_593437.1 homoaconitate hydratase Lys2; EC 4.2.1.36 from Schizosaccharomyces pombe (see 2 papers)
    30% identity, 92% coverage of query (149 bits)

lysF / GI|1628374 homoaconitase, mitochondrial; EC 4.2.1.36 from Emericella nidulans (see paper)
    28% identity, 93% coverage of query (147 bits)

HtyD / K0E2G4 2-(4-hydroxybenzyl)-malate isomerase from Aspergillus rugulosus (see paper)
HTYD_ASPRU / K0E2G4 Aconitase htyD; L-homotyrosine biosynthetic cluster protein D; EC 4.2.1.- from Aspergillus rugulosus (Emericella rugulosa) (see paper)
    28% identity, 89% coverage of query (147 bits)

B1P2N4 homoaconitate hydratase (EC 4.2.1.36) from Penicillium chrysogenum (see paper)
    28% identity, 93% coverage of query (146 bits)

ACON_YEAST / P19414 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 8 papers)
P19414 aconitate hydratase (EC 4.2.1.3) from Saccharomyces cerevisiae (see 2 papers)
    29% identity, 86% coverage of query (146 bits)

ACON2_SCHPO / Q9P7D4 Aconitase-ribosomal protein bL21m fusion protein; EC 4.2.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
Q9P7D4 aconitate hydratase (EC 4.2.1.3) from Schizosaccharomyces pombe (see paper)
    29% identity, 86% coverage of query (144 bits)

ACON_EMENI / C8VG90 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; Homocitrate dehydratase; EC 4.2.1.3; EC 4.2.1.- from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
    29% identity, 86% coverage of query (143 bits)

LYS4_YEAST / P49367 Homoaconitase, mitochondrial; Homoaconitate hydratase; EC 4.2.1.36 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
P49367 homoaconitate hydratase (EC 4.2.1.36) from Saccharomyces cerevisiae (see paper)
    27% identity, 88% coverage of query (143 bits)

LYS4_ASPFU / Q4WUL6 Homoaconitase, mitochondrial; Homoaconitate hydratase; EC 4.2.1.36 from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
    27% identity, 95% coverage of query (142 bits)

J9VIU5 methanogen homoaconitase (EC 4.2.1.114) from Cryptococcus neoformans var. grubii serotype A (see paper)
    26% identity, 94% coverage of query (139 bits)

ACON3_ASPFU / Q4WBR0 Putative aconitate hydratase; Aconitase 3; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
    29% identity, 89% coverage of query (139 bits)

Q8RP87 aconitate hydratase (EC 4.2.1.3) from Bacteroides fragilis (see paper)
    29% identity, 86% coverage of query (137 bits)

GLOJ_GLAL2 / S3E7P8 3-isopropylmalate dehydratase large subunit gloJ; L-homotyrosine biosynthesis sub-cluster protein gloJ; Pneumocandin biosynthesis cluster protein J; EC 4.2.1.33 from Glarea lozoyensis (strain ATCC 20868 / MF5171) (see 5 papers)
    26% identity, 89% coverage of query (135 bits)

Aco / O74699 aconitase from Aspergillus terreus (see paper)
    27% identity, 86% coverage of query (135 bits)

ACON2_EMENI / Q5B6D6 Putative aconitate hydratase, mitochondrial; Aconitase 2; EC 4.2.1.- from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
    28% identity, 86% coverage of query (134 bits)

ACO1 aconitate hydratase; EC 4.2.1.3 from Candida albicans (see 2 papers)
    29% identity, 86% coverage of query (134 bits)

ACON_ASPFU / Q4WLN1 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; Homocitrate dehydratase; EC 4.2.1.3; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
    27% identity, 86% coverage of query (134 bits)

ACON2_ASPFU / Q4WJ90 Putative aconitate hydratase, mitochondrial; Aconitase 2; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
    30% identity, 86% coverage of query (132 bits)

ACON_SCHPO / O13966 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    29% identity, 86% coverage of query (132 bits)

ACON_CAEEL / P34455 Probable aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Caenorhabditis elegans (see paper)
    29% identity, 86% coverage of query (130 bits)

1nisA Crystal structure of aconitase with trans-aconitate and nitrocitrate bound
    29% identity, 86% coverage of query (115 bits)

8acnA Crystal structures of aconitase with isocitrate and nitroisocitrate bound
    29% identity, 86% coverage of query (115 bits)

1fghA Complex with 4-hydroxy-trans-aconitate
    29% identity, 86% coverage of query (115 bits)

1amjA Steric and conformational features of the aconitase mechanism
    29% identity, 86% coverage of query (115 bits)

1amiA Steric and conformational features of the aconitase mechanism
    29% identity, 86% coverage of query (115 bits)

1acoA Crystal structure of aconitase with transaconitate bound
    29% identity, 86% coverage of query (115 bits)

ACON_BOVIN / P20004 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Bos taurus (Bovine) (see paper)
    28% identity, 86% coverage of query (114 bits)

1b0kA S642a:fluorocitrate complex of aconitase
    29% identity, 86% coverage of query (114 bits)

ACON_PIG / P16276 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Sus scrofa (Pig) (see 4 papers)
    29% identity, 86% coverage of query (114 bits)

5acnA / P16276 Structure of activated aconitase. Formation of the (4fe-4s) cluster in the crystal (see paper)
    29% identity, 86% coverage of query (114 bits)

ACO1 / P21399 cytoplasmic aconitate hydratase from Homo sapiens (see 6 papers)
ACOHC_HUMAN / P21399 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Homo sapiens (Human) (see 8 papers)
P21399 aconitate hydratase (EC 4.2.1.3) from Homo sapiens (see 3 papers)
    27% identity, 86% coverage of query (107 bits)

2b3xA / P21399 Structure of an orthorhombic crystal form of human cytosolic aconitase (irp1) (see paper)
    27% identity, 86% coverage of query (107 bits)

ACO3M_ARATH / Q9SIB9 Aconitate hydratase 3, mitochondrial; Aconitase 3; mACO1; Citrate hydro-lyase 3; EC 4.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
Q9SIB9 aconitate hydratase (EC 4.2.1.3) from Arabidopsis thaliana (see paper)
    25% identity, 86% coverage of query (105 bits)

citB / P09339 aconitate hydratase (bifunctional aconitase) (EC 4.2.1.99; EC 4.2.1.3) from Bacillus subtilis (strain 168) (see 21 papers)
ACNA_BACSU / P09339 Aconitate hydratase A; ACN; Aconitase; Aconitate/2-methylaconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.- from Bacillus subtilis (strain 168) (see 7 papers)
P09339 aconitate hydratase (EC 4.2.1.3) from Bacillus subtilis (see paper)
    28% identity, 76% coverage of query (105 bits)

A0QX20 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis)
    25% identity, 78% coverage of query (71.2 bits)

3snpA / Q01059 Crystal structure analysis of iron regulatory protein 1 in complex with ferritin h ire RNA (see paper)
    26% identity, 77% coverage of query (70.9 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory