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Searching for up to 100 curated homologs for WP_013259630.1 NCBI__GCF_000143965.1:WP_013259630.1 (638 a.a.)

Found high-coverage hits (≥70%) to 18 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

hytB / S5YUD3 hydrogenase (NADP+,ferredoxin) β subunit from Clostridium autoethanogenum DSM 10061 (see paper)
    57% identity, 93% coverage of query (696 bits)

hydB / Q8RBC9 NADH-dependent hydrogenase β subunit (EC 1.12.1.2) from Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (see 2 papers)
    58% identity, 93% coverage of query (693 bits)

8a6tB / A0A097ATG4 Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
    54% identity, 96% coverage of query (684 bits)

7q4vB / H6LFG4 Electron bifurcating hydrogenase - hydabc from a. Woodii (see paper)
    57% identity, 93% coverage of query (681 bits)

hymB / GI|14250934 protein HymB from Eubacterium acidaminophilum (see paper)
    55% identity, 93% coverage of query (645 bits)

K0B622 formate dehydrogenase (NAD+, ferredoxin) (subunit 2/4) (EC 1.17.1.11) from Gottschalkia acidurici (see paper)
    54% identity, 93% coverage of query (642 bits)

8oh5B / A0A0U1KYM9 Cryo-em structure of the electron bifurcating transhydrogenase stnabc complex from sporomusa ovata (state 2) (see paper)
    56% identity, 92% coverage of query (625 bits)

TC 3.D.1.5.1 / Q74GA3 NuoF aka GSU0343, component of Proton-translocating NADH dehydrogenase I from Geobacter sulfurreducens (see paper)
    53% identity, 92% coverage of query (609 bits)

7p8nB / O52682 Tmhydabc- t. Maritima hydrogenase with bridge closed (see paper)
    50% identity, 95% coverage of query (592 bits)

HNDC_SOLFR / Q46507 NADP-reducing hydrogenase subunit HndC; Hydrogen dehydrogenase (NADP(+)); EC 1.12.1.3 from Solidesulfovibrio fructosivorans (Desulfovibrio fructosivorans) (see 2 papers)
    58% identity, 75% coverage of query (591 bits)

hydB / O52682 hydrogenase β subunit (EC 1.12.1.4) from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see 2 papers)
HYDB_THEMA / O52682 Bifurcating [FeFe] hydrogenase beta subunit; Hydrogenase (NAD(+), ferredoxin) beta subunit; Iron-hydrogenase beta subunit; EC 1.12.1.4 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see 2 papers)
    49% identity, 95% coverage of query (588 bits)

7q4vF Electron bifurcating hydrogenase - hydabc from a. Woodii
    63% identity, 73% coverage of query (587 bits)

Q84FW2 formate dehydrogenase (EC 1.17.1.9) from Methylorubrum extorquens (see paper)
    40% identity, 80% coverage of query (372 bits)

hoxF / P22317 α subunit from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (see paper)
P22317 hydrogen dehydrogenase (EC 1.12.1.2) from Cupriavidus necator (see paper)
    35% identity, 82% coverage of query (294 bits)

7vw6B / C5ATT6 Cryo-em structure of formate dehydrogenase 1 from methylorubrum extorquens am1 (see paper)
    31% identity, 82% coverage of query (234 bits)

fdh1B / Q8KTI8 NAD-dependent formate dehydrogenase β subunit (EC 1.17.1.9) from Methylorubrum extorquens (see 2 papers)
Q8KTI8 formate dehydrogenase (EC 1.17.1.9) from Methylorubrum extorquens (see paper)
    31% identity, 82% coverage of query (234 bits)

5xfaA / A0A077L6X8 Crystal structure of NAD+-reducing [nife]-hydrogenase in the h2- reduced state (see paper)
    35% identity, 71% coverage of query (231 bits)

5xf9A Crystal structure of NAD+-reducing [nife]-hydrogenase in the air- oxidized state
    35% identity, 71% coverage of query (231 bits)

Build an alignment

Build an alignment for WP_013259630.1 and 18 homologs with ≥ 30% identity

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory