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Searching for up to 100 curated homologs for WP_013420474.1 NCBI__GCF_000166055.1:WP_013420474.1 (695 a.a.)

Found high-coverage hits (≥70%) to 50 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

6iunB / Q0KBG3 Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
    46% identity, 98% coverage of query (622 bits)

pimF / Q6N3H7 6-carboxyhex-2-enoyl-CoA hydratase from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) (see paper)
    47% identity, 98% coverage of query (621 bits)

H281DRAFT_00361 (3S)-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) / enoyl-CoA hydratase domain from Paraburkholderia bryophila 376MFSha3.1
    47% identity, 98% coverage of query (591 bits)

BPHYT_RS13545 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) from Burkholderia phytofirmans PsJN
    46% identity, 98% coverage of query (583 bits)

EHHADH / Q08426 peroxisomal bifunctional enzyme (EC 4.2.1.17; EC 1.1.1.35) from Homo sapiens (see 14 papers)
ECHP_HUMAN / Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 4 papers)
    41% identity, 98% coverage of query (485 bits)

3zwaA / P07896 Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-hexanoyl-coa (see paper)
    40% identity, 98% coverage of query (470 bits)

ECHP_RAT / P07896 Peroxisomal bifunctional enzyme; PBE; PBFE; Multifunctional enzyme 1; MFE1; Multifunctional protein 1; MFP1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Rattus norvegicus (Rat) (see 4 papers)
P07896 enoyl-CoA hydratase (EC 4.2.1.17) from Rattus norvegicus (see paper)
Ehhadh / RF|NP_598290.1 noyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase/3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (see paper)
    40% identity, 98% coverage of query (470 bits)

3zw9A Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2-methylbutanoyl-coa
    40% identity, 98% coverage of query (470 bits)

5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
    40% identity, 98% coverage of query (470 bits)

5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD
    40% identity, 98% coverage of query (470 bits)

3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa
    40% identity, 98% coverage of query (470 bits)

2x58A The crystal structure of mfe1 liganded with coa
    40% identity, 98% coverage of query (470 bits)

6zibAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and nadh'
    41% identity, 98% coverage of query (469 bits)

6zicAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with 3s-hydroxybutanoyl-coa and nadh'
    40% identity, 98% coverage of query (468 bits)

6z5oAAA Peroxisomal bifunctional enzyme
    40% identity, 98% coverage of query (468 bits)

3zwbA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 2trans-hexenoyl-coa
    40% identity, 98% coverage of query (467 bits)

ECHP_MOUSE / Q9DBM2 Peroxisomal bifunctional enzyme; PBE; PBFE; L-peroxisomal bifunctional enzyme; L-PBE; Multifunctional enzyme 1; MFE1; Multifunctional protein 1; MFP-1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Mus musculus (Mouse) (see 2 papers)
    39% identity, 98% coverage of query (463 bits)

MFP2 / Q9ZPI5 peroxisomal fatty acid β-oxidation multifunctional protein MFP2 (EC 1.1.1.35; EC 4.2.1.17) from Arabidopsis thaliana (see 4 papers)
MFP2_ARATH / Q9ZPI5 Peroxisomal fatty acid beta-oxidation multifunctional protein MFP2; AtMPF2; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Arabidopsis thaliana (Mouse-ear cress) (see 7 papers)
Q9ZPI5 enoyl-CoA hydratase (EC 4.2.1.17) from Arabidopsis thaliana (see paper)
    37% identity, 97% coverage of query (426 bits)

AIM1 / Q9ZPI6 peroxisomal fatty acid β-oxidation multifunctional protein AIM1 (EC 1.1.1.35; EC 4.2.1.17) from Arabidopsis thaliana (see 4 papers)
AIM1_ARATH / Q9ZPI6 Peroxisomal fatty acid beta-oxidation multifunctional protein AIM1; Protein ABNORMAL INFLORESCENCE MERISTEM 1; AtAIM1; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
    36% identity, 98% coverage of query (417 bits)

MFPA_CUCSA / Q39659 Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Cucumis sativus (Cucumber) (see paper)
    35% identity, 97% coverage of query (413 bits)

MFP_ORYSJ / Q8W1L6 Peroxisomal fatty acid beta-oxidation multifunctional protein; MFP; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Oryza sativa subsp. japonica (Rice) (see paper)
    36% identity, 98% coverage of query (404 bits)

ECHA_RAT / Q64428 Trifunctional enzyme subunit alpha, mitochondrial; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Rattus norvegicus (Rat) (see paper)
Q64428 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211); acetyl-CoA C-acyltransferase (EC 2.3.1.16) from Rattus norvegicus (see 6 papers)
    32% identity, 100% coverage of query (335 bits)

ECHA_MOUSE / Q8BMS1 Trifunctional enzyme subunit alpha, mitochondrial; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Mus musculus (Mouse) (see paper)
    31% identity, 98% coverage of query (330 bits)

HADHA / P40939 mitochondrial trifunctional enzyme α subunit (EC 1.1.1.35; EC 4.2.1.17; EC 1.1.1.211) from Homo sapiens (see 5 papers)
ECHA_HUMAN / P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 11 papers)
P40939 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) from Homo sapiens (see 6 papers)
    31% identity, 98% coverage of query (329 bits)

FadJ / b2341 3-hydroxyacyl-CoA dehydrogenase FadJ (EC 1.1.1.35; EC 1.1.1.211; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8) from Escherichia coli K-12 substr. MG1655 (see 8 papers)
fadJ / P77399 multifunctional 3-hydroxyacyl-CoA dehydrogenase/3-hydroxyacyl-CoA epimerase/enoyl-CoA hydratase FadJ (EC 1.1.1.35; EC 4.2.1.17; EC 4.2.1.74; EC 5.1.2.3; EC 5.3.3.8) from Escherichia coli (strain K12) (see 6 papers)
FADJ_ECOLI / P77399 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
fadJ / RF|NP_416843 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 from Escherichia coli K12 (see 8 papers)
    34% identity, 98% coverage of query (324 bits)

6yswA / P77399 E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
    34% identity, 98% coverage of query (323 bits)

HP15_1512 Enoyl-CoA hydratase (EC 4.2.1.17) from Marinobacter adhaerens HP15
    32% identity, 92% coverage of query (308 bits)

fadB / Q9HZJ2 fatty acid oxidation complex α subunit (EC 1.1.1.35; EC 4.2.1.74) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
    34% identity, 94% coverage of query (302 bits)

fadB / Q88L02 FadB (EC 5.3.3.8; EC 1.1.1.35; EC 5.1.2.3; EC 4.2.1.17; EC 1.1.1.36) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 3 papers)
    33% identity, 95% coverage of query (300 bits)

FADB_PSEFR / P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see 2 papers)
1wdlA / P28793 Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
    33% identity, 95% coverage of query (297 bits)

OldB / b3846 multifunctional enoyl-CoA hydratase, 3-hydroxyacyl-CoA epimerase, Δ3-cis- Δ2-trans-enoyl-CoA isomerase, L-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35; EC 1.1.1.211; EC 4.2.1.17; EC 5.3.3.8; EC 5.1.2.3) from Escherichia coli K-12 substr. MG1655 (see 36 papers)
fadB / P21177 multifunctional enoyl-CoA hydratase, 3-hydroxyacyl-CoA epimerase, Δ3-cis- Δ2-trans-enoyl-CoA isomerase, L-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35; EC 4.2.1.17; EC 4.2.1.74; EC 5.3.3.8; EC 5.1.2.3) from Escherichia coli (strain K12) (see 36 papers)
FADB_ECOLI / P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 5 papers)
    32% identity, 98% coverage of query (295 bits)

6tnmA / P21177 E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
    32% identity, 98% coverage of query (295 bits)

1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3)
    33% identity, 95% coverage of query (291 bits)

Sama_0032 Enoyl-CoA hydratase (EC 4.2.1.17); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) from Shewanella amazonensis SB2B
    33% identity, 94% coverage of query (270 bits)

7o4uA Structure of the alpha subunit of mycobacterium tuberculosis beta- oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide
    32% identity, 92% coverage of query (262 bits)

Shewana3_0024 Enoyl-CoA hydratase (EC 4.2.1.17); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) from Shewanella sp. ANA-3
    32% identity, 95% coverage of query (261 bits)

8pf8A Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-72
    31% identity, 92% coverage of query (259 bits)

8oquA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-92
    31% identity, 92% coverage of query (259 bits)

8oqtA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-91
    31% identity, 92% coverage of query (259 bits)

8oqnA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-53
    31% identity, 92% coverage of query (259 bits)

8opvA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with resveratrol (fragment-b-h11)
    31% identity, 92% coverage of query (259 bits)

8opuA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with sulfamethoxazole (fragment-b-e1)
    31% identity, 92% coverage of query (259 bits)

8oqoA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-49
    31% identity, 92% coverage of query (259 bits)

8oqqA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-79
    31% identity, 92% coverage of query (259 bits)

8oqpA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-76
    31% identity, 92% coverage of query (259 bits)

8oqsB / O53872 Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-83 (see paper)
    31% identity, 92% coverage of query (258 bits)

8oqrA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-80
    31% identity, 92% coverage of query (258 bits)

4b3iA Crystal structure of mycobacterium tuberculosis fatty acid beta- oxidation complex with coenzymea bound at the hydratase active sites
    31% identity, 92% coverage of query (258 bits)

8oqlA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-1
    31% identity, 92% coverage of query (258 bits)

8oqvA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-109
    31% identity, 92% coverage of query (258 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory