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Searching for up to 100 curated homologs for WP_013707581.1 NCBI__GCF_000195295.1:WP_013707581.1 (354 a.a.)

Found high-coverage hits (≥70%) to 39 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

6bwcC X-ray structure of pen from bacillus thuringiensis (see paper)
    43% identity, 95% coverage of query (288 bits)

BC_3750 / Q81A42 UDP-N-acetylglucosamine 4,6-dehydratase (EC 4.2.1.135) from Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711) (see 5 papers)
Q81A42 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (EC 4.2.1.135) from Bacillus cereus (see 2 papers)
    43% identity, 81% coverage of query (240 bits)

PSEB_CAMJJ / Q5QKR8 UDP-N-acetylglucosamine 4,6-dehydratase (inverting); Pseudaminic acid biosynthesis protein B; UDP-GlcNAc-inverting 4,6-dehydratase; EC 4.2.1.115 from Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) (see 2 papers)
    38% identity, 84% coverage of query (221 bits)

EPSC_BACSU / P71052 Probable polysaccharide biosynthesis protein EpsC from Bacillus subtilis (strain 168) (see paper)
    44% identity, 79% coverage of query (220 bits)

PSEB_CAMJE / Q0P8W4 UDP-N-acetylglucosamine 4,6-dehydratase (inverting); Pseudaminic acid biosynthesis protein B; UDP-GlcNAc-inverting 4,6-dehydratase; EC 4.2.1.115 from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) (see paper)
    38% identity, 83% coverage of query (219 bits)

cpsE polysaccharide capsule synthesis protein CpsE from Streptococcus iniae (see paper)
    44% identity, 78% coverage of query (219 bits)

wbiI / GI|3135688 putative epimerase/dehydratase WbiI from Burkholderia pseudomallei (see paper)
    41% identity, 79% coverage of query (216 bits)

Q6VYQ6 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Helicobacter pylori (see paper)
    38% identity, 87% coverage of query (216 bits)

pseB / O25511 UDP-N-acetylglucosamine 4,6-dehydratase subunit (EC 4.2.1.115) from Helicobacter pylori (strain ATCC 700392 / 26695) (see 4 papers)
PSEB_HELPY / O25511 UDP-N-acetylglucosamine 4,6-dehydratase (inverting); Pseudaminic acid biosynthesis protein B; UDP-GlcNAc-inverting 4,6-dehydratase; EC 4.2.1.115 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see 3 papers)
    38% identity, 87% coverage of query (215 bits)

2gn4A / O25511 Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADPH and udp-glcnac (see paper)
    39% identity, 85% coverage of query (215 bits)

Q6VYQ5 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Helicobacter pylori (see paper)
    38% identity, 87% coverage of query (215 bits)

2gnaA Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-gal
    39% identity, 85% coverage of query (215 bits)

2gn9A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-glc
    39% identity, 85% coverage of query (215 bits)

2gn6A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-glcnac
    39% identity, 85% coverage of query (215 bits)

V5RBP5 UDP-glucose 4-epimerase (EC 5.1.3.2) from Acinetobacter baumannii (see paper)
    36% identity, 97% coverage of query (214 bits)

2gn8A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp
    39% identity, 85% coverage of query (214 bits)

4j2oC / P37362 Crystal structure of NADP-bound wbjb from a. Baumannii community strain d1279779 (see paper)
    40% identity, 82% coverage of query (213 bits)

wbjB / Q9KID0 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Pseudomonas aeruginosa (see 2 papers)
    35% identity, 96% coverage of query (209 bits)

wbvB / Q8L348 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Vibrio cholerae O37 (see 3 papers)
    34% identity, 97% coverage of query (208 bits)

A0A0H3JPH0 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Staphylococcus aureus (see paper)
    34% identity, 94% coverage of query (199 bits)

RfbU / CAA69127.1 mannosyl-transferase from Vibrio cholerae (see paper)
    40% identity, 79% coverage of query (198 bits)

3w1vA / A0A0H3JPH0 Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with inihibitor (see paper)
    34% identity, 94% coverage of query (198 bits)

4g5hA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with by-product
    34% identity, 94% coverage of query (198 bits)

3vvcA Crystal structure of capsular polysaccharide synthesizing enzyme cape , k126e, in apo form
    36% identity, 82% coverage of query (195 bits)

wbgZ / Q9F736 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (EC 4.2.1.135) from Shigella sonnei (see paper)
    39% identity, 77% coverage of query (191 bits)

P72145 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Pseudomonas aeruginosa (see paper)
    37% identity, 76% coverage of query (190 bits)

wbpM / Q9KIC5 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (EC 4.2.1.135) from Pseudomonas aeruginosa (see 6 papers)
    37% identity, 76% coverage of query (189 bits)

wbkD / Q2YMM0 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (EC 4.2.1.135) from Brucella abortus (strain 2308) (see 2 papers)
    39% identity, 77% coverage of query (188 bits)

CAPD_RICPR / Q9ZDJ5 UDP-glucose 4-epimerase; Galactowaldenase; UDP-galactose 4-epimerase; EC 5.1.3.2 from Rickettsia prowazekii (strain Madrid E) (see paper)
    37% identity, 79% coverage of query (186 bits)

5bjuA / O86159 X-ray structure of the pglf dehydratase from campylobacter jejuni in complex with udp and NAD(h) (see paper)
    39% identity, 78% coverage of query (178 bits)

pglF / Q0P9D4 UDP-N-acetylglucosamine C-6 dehydratase (EC 4.2.1.135) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) (see 3 papers)
PGLF_CAMJE / Q0P9D4 UDP-N-acetyl-alpha-D-glucosamine C6 dehydratase; UDP-GlcNAc C6 dehydratase; Protein glycosylation pathway protein F; UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining); EC 4.2.1.135 from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) (see paper)
    39% identity, 78% coverage of query (177 bits)

5bjvA X-ray structure of the pglf udp-n-acetylglucosamine 4,6-dehydratase from campylobacterjejuni, d396n/k397a variant in complex with udp-n- acrtylglucosamine
    39% identity, 78% coverage of query (174 bits)

G5CSR9 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) (EC 4.2.1.115) from Megavirus chiliensis (see paper)
    30% identity, 94% coverage of query (143 bits)

3vvbA / A0A0H3JPH0 Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in apo form (see paper)
    31% identity, 70% coverage of query (135 bits)

Build an alignment

Build an alignment for WP_013707581.1 and 34 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

4tqgA / G5CSR9 Crystal structure of megavirus udp-glcnac 4,6-dehydratase, 5-epimerase mg534 (see paper)
    28% identity, 93% coverage of query (125 bits)

graE / AAA99939.1 dTDP-glucose dehydratase from Streptomyces violaceoruber (see paper)
    27% identity, 72% coverage of query (65.5 bits)

6dntA / D3E402 Udp-n-acetylglucosamine 4-epimerase from methanobrevibacter ruminantium m1 in complex with udp-n-acetylmuramic acid (see paper)
    26% identity, 77% coverage of query (65.1 bits)

RHM2 / Q9LPG6 trifunctional UDP-glucose 4,6-dehydratase/UDP-4-dehydro-6-deoxy-D-glucose 3,5-epimerase/UDP-4-dehydro-L-rhamnose-reductase RHM2 (EC 4.2.1.76) from Arabidopsis thaliana (see 2 papers)
RHM2_ARATH / Q9LPG6 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM2; NDP-rhamnose synthase; Protein MUCILAGE-MODIFIED 4; Protein RHAMNOSE BIOSYNTHESIS 2; Rhamnose biosynthetic enzyme 2; AtRHM2; UDP-L-rhamnose synthase MUM4; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
    26% identity, 72% coverage of query (58.9 bits)

3β-HSD/D1 / Q9FX01 plant 3β-hydroxysteroid dehydrogenase/C4-decarboxylase 1 (EC 1.1.1.418) from Arabidopsis thaliana (see 3 papers)
HSDD1_ARATH / Q9FX01 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1; At3BETAHSD/D1; 4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase isoform 1-1; Reticulon-like protein B24; AtRTNLB24; Sterol-4-alpha-carboxylate 3-dehydrogenase 1, decarboxylating; EC 1.1.1.418 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
    25% identity, 71% coverage of query (51.2 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory