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Searching for up to 100 curated homologs for WP_015738277.1 NCBI__GCF_000024605.1:WP_015738277.1 (522 a.a.)

Found high-coverage hits (≥70%) to 37 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

cimA / Q74C76 (R)-citramalate synthase (EC 2.3.3.21) from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) (see paper)
CIMA_GEOSL / Q74C76 (R)-citramalate synthase; Citramalate synthase; EC 2.3.3.21 from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) (see paper)
Q74C76 (R)-citramalate synthase (EC 2.3.3.21) from Geobacter sulfurreducens (see paper)
    54% identity, 100% coverage of query (574 bits)

B0K6M2 (R)-citramalate synthase (EC 2.3.3.21) from Thermoanaerobacter sp. (see paper)
    51% identity, 100% coverage of query (551 bits)

DVU1914 (R)-citramalate synthase (EC 2.3.3.21) from Desulfovibrio vulgaris Hildenborough JW710
    50% identity, 99% coverage of query (496 bits)

CIMA_SULAC / Q4J6H1 (R)-citramalate synthase; Citramalate synthase; CMS; EC 2.3.3.21 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
    49% identity, 98% coverage of query (492 bits)

LEU1_METJA / Q58595 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58595 2-isopropylmalate synthase (EC 2.3.3.13) from Methanocaldococcus jannaschii (see paper)
    30% identity, 99% coverage of query (222 bits)

cimA / Q58787 (R)-citratemalate synthase subunit (EC 2.3.3.21) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see paper)
CIMA_METJA / Q58787 (R)-citramalate synthase CimA; EC 2.3.3.21 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58787 (R)-citramalate synthase (EC 2.3.3.21) from Methanocaldococcus jannaschii (see 2 papers)
    31% identity, 98% coverage of query (216 bits)

LEU1_SULAC / Q4JA78 2-isopropylmalate synthase; IPMS; Alpha-isopropylmalate synthase; Alpha-IPM synthase; EC 2.3.3.13 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
    35% identity, 72% coverage of query (213 bits)

Build an alignment

Build an alignment for WP_015738277.1 and 7 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

Q72JC9 2-isopropylmalate synthase (EC 2.3.3.13) from Thermus thermophilus (see 2 papers)
    29% identity, 98% coverage of query (189 bits)

AKSA_METJA / Q57926 Homocitrate synthase AksA; (R)-homo(2)citrate synthase; (R)-homo(3)citrate synthase; EC 2.3.3.14; EC 2.3.3.- from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
    31% identity, 71% coverage of query (187 bits)

LEU1_SALTY / P15875 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 3 papers)
    29% identity, 97% coverage of query (184 bits)

LeuA / b0074 2-isopropylmalate synthase (EC 2.3.3.13) from Escherichia coli K-12 substr. MG1655 (see 7 papers)
leuA / P09151 2-isopropylmalate synthase (EC 2.3.3.13) from Escherichia coli (strain K12) (see 15 papers)
    30% identity, 97% coverage of query (184 bits)

6e1jA / C5J4P1 Crystal structure of methylthioalkylmalate synthase (bjumam1.1) from brassica juncea (see paper)
    30% identity, 70% coverage of query (172 bits)

BT1861 2-isopropylmalate synthase (EC 2.3.3.13) from Bacteroides thetaiotaomicron VPI-5482
    26% identity, 98% coverage of query (171 bits)

LEU1_NEIMB / Q9JZG1 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Neisseria meningitidis serogroup B (strain MC58) (see 2 papers)
Q9JZG1 2-isopropylmalate synthase (EC 2.3.3.13) from Neisseria meningitidis (see 2 papers)
    28% identity, 85% coverage of query (170 bits)

LEU1_SOYBN / Q39891 Probable 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; Late nodulin-56; N-56; EC 2.3.3.13 from Glycine max (Soybean) (Glycine hispida) (see paper)
    27% identity, 92% coverage of query (162 bits)

MAM3 / Q9FN52 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see paper)
MAM3_ARATH / Q9FN52 Methylthioalkylmalate synthase 3, chloroplastic; 2-isopropylmalate synthase 2; Methylthioalkylmalate synthase-like; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9FN52 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see 4 papers)
    30% identity, 72% coverage of query (161 bits)

K4C627 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum lycopersicum (see paper)
    26% identity, 97% coverage of query (161 bits)

cimA / Q8F3Q1 (R)-citramalate synthase subunit (EC 2.3.3.21) from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see paper)
CIMA_LEPIN / Q8F3Q1 (R)-citramalate synthase CimA; LiCMS; EC 2.3.3.21 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see 3 papers)
    27% identity, 95% coverage of query (155 bits)

LEU11_LEPIN / Q8F445 2-isopropylmalate synthase 1; Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1; EC 2.3.3.13 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see paper)
    27% identity, 97% coverage of query (152 bits)

LEU12_ARATH / Q9C550 2-isopropylmalate synthase 2, chloroplastic; 2-isopropylmalate synthase 1; Methylthioalkylmalate synthase-like 3; EC 2.3.3.13 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9C550 2-isopropylmalate synthase (EC 2.3.3.13) from Arabidopsis thaliana (see 2 papers)
    27% identity, 97% coverage of query (151 bits)

BT1858 (R)-citramalate synthase (EC 2.3.1.182) from Bacteroides thetaiotaomicron VPI-5482
    29% identity, 96% coverage of query (150 bits)

LEU11_ARATH / Q9LPR4 2-isopropylmalate synthase 1, chloroplastic; Methylthioalkylmalate synthase-like 4; EC 2.3.3.13 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
Q9LPR4 2-isopropylmalate synthase (EC 2.3.3.13) from Arabidopsis thaliana (see 3 papers)
    27% identity, 97% coverage of query (150 bits)

D0VY45 homocitrate synthase (EC 2.3.3.14) from Lotus japonicus (see paper)
    27% identity, 93% coverage of query (145 bits)

Q30DX9 2-isopropylmalate synthase (EC 2.3.3.13) from Brassica insularis (see paper)
    27% identity, 97% coverage of query (145 bits)

A0A1Y6KBA1 homocitrate synthase (EC 2.3.3.14) from Bradyrhizobium sp. ORS 285 (see 2 papers)
    28% identity, 70% coverage of query (132 bits)

LEU12_LEPIN / Q8F8T4 2-isopropylmalate synthase 2; Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2; EC 2.3.3.13 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see 2 papers)
    29% identity, 73% coverage of query (119 bits)

C7C437 homocitrate synthase (EC 2.3.3.14) from Aspergillus fumigatus (see paper)
    25% identity, 75% coverage of query (113 bits)

hphA / B2J8P8 2-benzylmalate synthase from Nostoc punctiforme (strain ATCC 29133 / PCC 73102) (see paper)
    26% identity, 73% coverage of query (104 bits)

LEU4 putative 2-isopropylmalalate synthase from Candida albicans (see paper)
    24% identity, 98% coverage of query (92.8 bits)

LEU9_YEAST / Q12166 2-isopropylmalate synthase 2, mitochondrial; Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2; Alpha-isopropylmalate synthase II; EC 2.3.3.13 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
    24% identity, 84% coverage of query (88.6 bits)

LEU1_YEAST / P06208 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
    24% identity, 96% coverage of query (87.8 bits)

LEU1_MYCTO / P9WQB2 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) (see paper)
LEU1_MYCTU / P9WQB3 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
P9WQB3 2-isopropylmalate synthase (EC 2.3.3.13) from Mycobacterium tuberculosis (see 6 papers)
    25% identity, 83% coverage of query (71.6 bits)

3hpsA Crystal structure of mycobacterium tuberculosis leua complexed with ketoisocaproate (kic)
    24% identity, 96% coverage of query (68.2 bits)

3figB / P9WQB3 Crystal structure of leucine-bound leua from mycobacterium tuberculosis (see paper)
    24% identity, 96% coverage of query (68.2 bits)

3hpzB Crystal structure of mycobacterium tuberculosis leua complexed with bromopyruvate
    24% identity, 96% coverage of query (68.2 bits)

3hq1A Crystal structure of mycobacterium tuberculosis leua complexed with citrate and mn2+
    24% identity, 96% coverage of query (67.8 bits)

1sr9A Crystal structure of leua from mycobacterium tuberculosis
    24% identity, 96% coverage of query (67.8 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory