Sites on a Tree

 

Searching for up to 100 curated homologs for WP_015942967.1 NCBI__GCF_000021925.1:WP_015942967.1 (460 a.a.)

Found high-coverage hits (≥70%) to 64 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

DVU_3027 / Q726S9 quinone-dependent D-lactate dehydrogenase catalytic subunit (EC 1.1.2.5) from Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / NCIMB 8303 / VKM B-1760 / Hildenborough) (see 3 papers)
    41% identity, 100% coverage of query (352 bits)

glcD1 / Q55124 glycolate dehydrogenase 1 (EC 1.1.99.14) from Synechocystis sp. (strain PCC 6803 / Kazusa) (see 4 papers)
Q55124 glycolate dehydrogenase (EC 1.1.99.14) from Synechocystis sp. PCC 6803 (see paper)
    40% identity, 98% coverage of query (345 bits)

Psest_3841 D-lactate/glycolate dehydrogenase, FAD-linked subunit GlcD (EC 1.1.99.6; EC 1.1.99.14) from Pseudomonas stutzeri RCH2
    35% identity, 98% coverage of query (310 bits)

b2979 glycolate dehydrogenase, putative FAD-linked subunit from Escherichia coli BW25113
YghM / b2979 glycolate dehydrogenase, putative FAD-linked subunit (EC 1.1.99.14) from Escherichia coli K-12 substr. MG1655 (see 9 papers)
glcD / P0AEP9 glycolate dehydrogenase, putative FAD-linked subunit (EC 1.1.99.14) from Escherichia coli (strain K12) (see 7 papers)
GLCD_ECOLI / P0AEP9 Glycolate oxidase subunit GlcD; Glycolate dehydrogenase subunit GlcD; EC 1.1.99.14 from Escherichia coli (strain K12) (see 4 papers)
glcD / RF|NP_417453 glycolate oxidase subunit glcD from Escherichia coli K12 (see paper)
glcD / AAB02530.1 glycolate oxidase subunit from Escherichia coli (see paper)
    35% identity, 98% coverage of query (296 bits)

SMc00832 D-lactate dehydrogenase, FAD-linked subunit GlcD (EC 1.1.99.6) from Sinorhizobium meliloti 1021
    36% identity, 99% coverage of query (286 bits)

PGA1_c29720 D-lactate dehydrogenase, FAD-linked subunit GlcD (EC 1.1.99.6) from Phaeobacter inhibens BS107
    34% identity, 98% coverage of query (286 bits)

E6N788 (S)-2-hydroxy-acid oxidase (EC 1.1.3.15); D-lactate dehydrogenase (acceptor) (EC 1.1.99.6) from Candidatus Caldarchaeum subterraneum (see paper)
    34% identity, 99% coverage of query (276 bits)

RR42_RS17300 D-lactate dehydrogenase, FAD-linked subunit GlcD (EC 1.1.99.6) from Cupriavidus basilensis FW507-4G11
    32% identity, 98% coverage of query (272 bits)

lctD / H6LBS1 lactate dehydrogenase (EC 1.1.1.436) from Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1) (see 2 papers)
LCTD_ACEWD / H6LBS1 Lactate dehydrogenase (NAD(+),ferredoxin) subunit LctD; EC 1.1.1.436 from Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1) (see paper)
H6LBS1 lactate dehydrogenase (NAD+, ferredoxin) (subunit 1/3) (EC 1.3.1.110) from Acetobacterium woodii (see paper)
    35% identity, 91% coverage of query (271 bits)

7qh2C / H6LBS1 Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
    35% identity, 91% coverage of query (271 bits)

DLD / Q94AX4 D-lactate dehydrogenase (cytochrome c) monomer (EC 1.1.2.4) from Arabidopsis thaliana (see 2 papers)
DLD_ARATH / Q94AX4 D-lactate dehydrogenase [cytochrome], mitochondrial; AtD-LDH; D-lactate ferricytochrome C oxidoreductase; Glycolate dehydrogenase; EC 1.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
    32% identity, 91% coverage of query (246 bits)

DLD_ANAHA / D4MUV9 D-lactate dehydrogenase; EC 1.1.99.6 from Anaerostipes hadrus (see paper)
    34% identity, 91% coverage of query (243 bits)

LDHD_DANRE / F1QXM5 Probable D-lactate dehydrogenase, mitochondrial; DLD; Lactate dehydrogenase D; EC 1.1.2.4 from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
    31% identity, 99% coverage of query (241 bits)

LDHD_MOUSE / Q7TNG8 Probable D-lactate dehydrogenase, mitochondrial; DLD; Lactate dehydrogenase D; EC 1.1.2.4 from Mus musculus (Mouse) (see paper)
    32% identity, 99% coverage of query (233 bits)

P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    34% identity, 98% coverage of query (233 bits)

LDHD / Q86WU2 mitochondrial D-lactate dehydrogenase (EC 1.1.2.4) from Homo sapiens (see 3 papers)
LDHD_HUMAN / Q86WU2 Probable D-lactate dehydrogenase, mitochondrial; DLD; Lactate dehydrogenase D; EC 1.1.2.4 from Homo sapiens (Human) (see 3 papers)
Q86WU2 D-lactate dehydrogenase (cytochrome) (EC 1.1.2.4) from Homo sapiens (see paper)
    32% identity, 91% coverage of query (232 bits)

A0A0G2K1W9 D-lactate dehydrogenase (EC 1.1.1.28) from Rattus norvegicus (see paper)
    32% identity, 99% coverage of query (231 bits)

8jdeA Crystal structure of mldhd in complex with d-lactate
    32% identity, 99% coverage of query (231 bits)

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid
    32% identity, 99% coverage of query (230 bits)

Q974L0 D-lactate dehydrogenase (acceptor) (EC 1.1.99.6) from Sulfurisphaera tokodaii (see paper)
    35% identity, 90% coverage of query (229 bits)

8jdsA Crystal structure of mldhd in complex with pyruvate
    32% identity, 99% coverage of query (229 bits)

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid
    32% identity, 99% coverage of query (228 bits)

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid
    31% identity, 99% coverage of query (228 bits)

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid
    32% identity, 99% coverage of query (226 bits)

8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid
    31% identity, 99% coverage of query (226 bits)

8jdbA / Q7TNG8 Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
    31% identity, 99% coverage of query (224 bits)

8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid
    31% identity, 99% coverage of query (224 bits)

8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid
    31% identity, 99% coverage of query (224 bits)

8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid
    31% identity, 99% coverage of query (224 bits)

8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid
    31% identity, 99% coverage of query (224 bits)

8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid
    31% identity, 99% coverage of query (224 bits)

8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid
    32% identity, 99% coverage of query (224 bits)

8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid
    32% identity, 99% coverage of query (224 bits)

Q9YEU4 D-lactate dehydrogenase (EC 1.1.1.28); D-lactate dehydrogenase (acceptor) (EC 1.1.99.6) from Aeropyrum pernix (see 2 papers)
    33% identity, 92% coverage of query (223 bits)

W1QLN6 D-lactate dehydrogenase (cytochrome) (EC 1.1.2.4) from Ogataea angusta (see 2 papers)
    30% identity, 99% coverage of query (221 bits)

G4MWZ3 D-lactate dehydrogenase (EC 1.1.1.28) from Pyricularia oryzae (see paper)
    31% identity, 99% coverage of query (211 bits)

DLD1 / P32891 D-lactate dehydrogenase (EC 1.1.2.4) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 11 papers)
DLD1_YEAST / P32891 D-lactate dehydrogenase [cytochrome] 1, mitochondrial; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
    30% identity, 98% coverage of query (209 bits)

G4RPF4 D-lactate dehydrogenase (acceptor) (EC 1.1.99.6) from Thermoproteus tenax (see paper)
    34% identity, 91% coverage of query (208 bits)

DLD_ARCFU / O29853 D-lactate dehydrogenase; EC 1.1.99.6 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see 2 papers)
O29853 D-lactate dehydrogenase (acceptor) (EC 1.1.99.6) from Archaeoglobus fulgidus (see 2 papers)
    32% identity, 90% coverage of query (196 bits)

Build an alignment

Build an alignment for WP_015942967.1 and 39 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

D2HDH_STUS1 / A4VGK4 D-2-hydroxyglutarate dehydrogenase; D-2-HG dehydrogenase; D2HGDH; D-malate dehydrogenase; EC 1.1.99.39; EC 1.1.99.- from Stutzerimonas stutzeri (strain A1501) (Pseudomonas stutzeri) (see 2 papers)
    28% identity, 100% coverage of query (184 bits)

Q9I6H4 D-2-hydroxyglutarate dehydrogenase (EC 1.1.99.39) from Pseudomonas aeruginosa (see paper)
    27% identity, 100% coverage of query (181 bits)

3pm9A / Q6NAV4 Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
    31% identity, 99% coverage of query (180 bits)

D2HDH_XANCL / P0DV35 D-2-hydroxyglutarate dehydrogenase; D2HGDH; EC 1.1.99.39 from Xanthomonas citri pv. viticola (strain LMG 965 / NCPPB 2475 / ICMP 3867 / CFBP 7660) (Xanthomonas campestris pv. viticola) (see paper)
    27% identity, 99% coverage of query (162 bits)

D2HDH_ARATH / O23240 D-2-hydroxyglutarate dehydrogenase, mitochondrial; AtD-2HGDH; EC 1.1.99.39 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
    27% identity, 98% coverage of query (160 bits)

6lpnB / Q8N465 Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
    26% identity, 91% coverage of query (149 bits)

6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og)
    26% identity, 91% coverage of query (149 bits)

6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg)
    26% identity, 91% coverage of query (149 bits)

6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal)
    26% identity, 91% coverage of query (149 bits)

6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg)
    26% identity, 91% coverage of query (149 bits)

D2HGDH / Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial (EC 1.1.99.39) from Homo sapiens (see paper)
D2HDH_HUMAN / Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
Q8N465 D-2-hydroxyglutarate dehydrogenase (EC 1.1.99.39) from Homo sapiens (see 3 papers)
    26% identity, 91% coverage of query (148 bits)

DLD2_YEAST / P46681 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2; D-2HG--pyruvate transhydrogenase DLD2; Actin-interacting protein 2; D-lactate dehydrogenase [cytochrome] 2, mitochondrial; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
    24% identity, 99% coverage of query (146 bits)

D2HDH_RAT / P84850 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Rattus norvegicus (Rat) (see paper)
    25% identity, 90% coverage of query (139 bits)

Rv0161 / O07406 trans-delta20-39-hydroxy-40-methyl-C60:1-[acp] oxidoreductase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
    26% identity, 90% coverage of query (135 bits)

DLD3_YEAST / P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
P39976 (R)-2-hydroxyglutarate-pyruvate transhydrogenase (EC 1.1.99.40) from Saccharomyces cerevisiae (see paper)
    25% identity, 90% coverage of query (134 bits)

D2HDH_PANAA / A0A0H3KZS3 D-2-hydroxyglutarate dehydrogenase; D2HGDH; EC 1.1.99.39 from Pantoea ananatis (strain AJ13355) (see paper)
    25% identity, 91% coverage of query (127 bits)

YdiJ / b1687 putative FAD-linked oxidoreductase YdiJ from Escherichia coli K-12 substr. MG1655 (see 4 papers)
ydiJ / P77748 D-2-hydroxyglutarate dehydrogenase (EC 1.1.99.39) from Escherichia coli (strain K12) (see 5 papers)
D2HDH_ECOLI / P77748 D-2-hydroxyglutarate dehydrogenase; D2HGDH; EC 1.1.99.39 from Escherichia coli (strain K12) (see 2 papers)
    25% identity, 91% coverage of query (124 bits)

YN53_SCHPO / Q9C1X2 Putative D-lactate dehydrogenase C713.03, mitochondrial; EC 1.1.2.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    24% identity, 99% coverage of query (123 bits)

PP_4493 D-2-hydroxyglutarate dehydrogenase (EC 1.1.99.39) from Pseudomonas putida KT2440
ydiJ / Q88EH0 (R)-2-hydroxyglutarate dehydrogenase (EC 1.1.99.39) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
D2HDH_PSEPK / Q88EH0 D-2-hydroxyglutarate dehydrogenase; D2HGDH; EC 1.1.99.39 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
    23% identity, 91% coverage of query (122 bits)

YgcV / b4463 putative FAD-containing dehydrogenase from Escherichia coli K-12 substr. MG1655 (see paper)
    26% identity, 98% coverage of query (121 bits)

ADAS_DICDI / O96759 Alkyldihydroxyacetonephosphate synthase; Alkyl-DHAP synthase; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Dictyostelium discoideum (Social amoeba) (see paper)
O96759 alkylglycerone-phosphate synthase (EC 2.5.1.26) from Dictyostelium discoideum (see paper)
    24% identity, 91% coverage of query (120 bits)

Q88DT2 D-2-hydroxyacid dehydrogenase (quinone) (EC 1.1.5.10); D-lactate dehydrogenase (quinone) (EC 1.1.5.12) from Pseudomonas putida (see paper)
    22% identity, 91% coverage of query (88.2 bits)

dld-II / Q8EGS3 D-lactate dehydrogenase Dld-II (EC 1.1.2.5) from Shewanella oneidensis (strain MR-1) (see paper)
    21% identity, 91% coverage of query (87.0 bits)

2bvfA / P08159 Crystal structure of 6-hydoxy-d-nicotine oxidase from arthrobacter nicotinovorans. Crystal form 3 (p1) (see paper)
    23% identity, 87% coverage of query (45.8 bits)

HDNO_PAENI / P08159 (R)-6-hydroxynicotine oxidase; 6-hydroxy-D-nicotine oxidase; 6-HDNO; D-6-hydroxynicotine oxidase; DHNO; EC 1.5.3.6 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see 10 papers)
    23% identity, 87% coverage of query (45.1 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory