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Searching for up to 100 curated homologs for WP_018125495.1 NCBI__GCF_000375485.1:WP_018125495.1 (455 a.a.)

Found high-coverage hits (≥70%) to 90 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

SYNO_ARATH / O48593 Asparagine--tRNA ligase, chloroplastic/mitochondrial; Asparaginyl-tRNA synthetase; AsnRS; AtNS1; Protein OVULE ABORTION 8; EC 6.1.1.22 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
    57% identity, 96% coverage of query (536 bits)

Tss / b0930 asparagine—tRNA ligase (EC 6.1.1.22) from Escherichia coli K-12 substr. MG1655 (see 13 papers)
asnS / P0A8M0 asparagine—tRNA ligase (EC 6.1.1.22) from Escherichia coli (strain K12) (see 14 papers)
SYN_ECOLI / P0A8M0 Asparagine--tRNA ligase; Asparaginyl-tRNA synthetase; AsnRS; EC 6.1.1.22 from Escherichia coli (strain K12) (see 2 papers)
    56% identity, 96% coverage of query (526 bits)

SYNM_HUMAN / Q96I59 Asparaginyl-tRNA synthetase; AsnRS; NARS2; Asparagine--tRNA ligase, mitochondrial; EC 6.1.1.22 from Homo sapiens (Human) (see 4 papers)
Q96I59 asparagine-tRNA ligase (EC 6.1.1.22) from Homo sapiens (see 2 papers)
    46% identity, 99% coverage of query (404 bits)

SYNM_MOUSE / Q8BGV0 Asparaginyl-tRNA synthetase; AsnRS; NARS2; Asparagine--tRNA ligase, mitochondrial; EC 6.1.1.22 from Mus musculus (Mouse) (see paper)
    45% identity, 98% coverage of query (400 bits)

SYNM_SCHPO / Q9P6I0 Asparagine--tRNA ligase, mitochondrial; Asparaginyl-tRNA synthetase; AsnRS; EC 6.1.1.22 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    42% identity, 95% coverage of query (330 bits)

SYNM_YEAST / P25345 Asparagine--tRNA ligase, mitochondrial; Asparaginyl-tRNA synthetase; AsnRS; EC 6.1.1.22 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
    39% identity, 95% coverage of query (309 bits)

P54263 asparagine-tRNA ligase (EC 6.1.1.22) from Thermus thermophilus (see paper)
    38% identity, 95% coverage of query (268 bits)

asnS / CAA62491.1 asparaginyl-tRNA synthetase from Thermus thermophilus (see paper)
    38% identity, 95% coverage of query (267 bits)

1x54A / O57980 Crystal structure of asparaginyl-tRNA synthetase from pyrococcus horikoshii complexed with asparaginyl-adenylate (see paper)
    35% identity, 96% coverage of query (229 bits)

1x55A Crystal structure of asparaginyl-tRNA synthetase from pyrococcus horikoshii complexed with asparaginyl-adenylate analogue
    35% identity, 96% coverage of query (229 bits)

SYNC_CAEEL / Q19722 Asparagine--tRNA ligase, cytoplasmic; Asparaginyl-tRNA synthetase; AsnRS; EC 6.1.1.22 from Caenorhabditis elegans (see paper)
    32% identity, 95% coverage of query (191 bits)

Build an alignment

Build an alignment for WP_018125495.1 and 11 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

SYNC_YEAST / P38707 Asparagine--tRNA ligase, cytoplasmic; Asparaginyl-tRNA synthetase; AsnRS; EC 6.1.1.22 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
    29% identity, 95% coverage of query (184 bits)

1b8aA / Q52428 Aspartyl-tRNA synthetase (see paper)
    31% identity, 100% coverage of query (184 bits)

SYD_THEKO / Q52428 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase; AspRS; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see 4 papers)
Q52428 aspartate-tRNA ligase (EC 6.1.1.12) from Thermococcus kodakarensis (see 4 papers)
    31% identity, 100% coverage of query (182 bits)

3nemB Aspartyl-tRNA synthetase complexed with aspartyl adenylate
    31% identity, 100% coverage of query (182 bits)

3nemA Aspartyl-tRNA synthetase complexed with aspartyl adenylate
    31% identity, 100% coverage of query (182 bits)

3nelA Aspartyl-tRNA synthetase complexed with aspartic acid
    31% identity, 100% coverage of query (182 bits)

2xgtB Asparaginyl-tRNA synthetase from brugia malayi complexed with the sulphamoyl analogue of asparaginyl-adenylate (see paper)
    30% identity, 95% coverage of query (176 bits)

2xtiA Asparaginyl-tRNA synthetase from brugia malayi complexed with atp:mg and l-asp-beta-noh adenylate:ppi:mg
    31% identity, 95% coverage of query (174 bits)

8h53A Human asparaginyl-tRNA synthetase in complex with asparagine-amp
    29% identity, 98% coverage of query (167 bits)

8tc9A Human asparaginyl-tRNA synthetase bound to osm-s-106
    29% identity, 95% coverage of query (167 bits)

2xtiB Asparaginyl-tRNA synthetase from brugia malayi complexed with atp:mg and l-asp-beta-noh adenylate:ppi:mg
    30% identity, 95% coverage of query (167 bits)

SYNC_HUMAN / O43776 Asparagine--tRNA ligase, cytoplasmic; Asparaginyl-tRNA synthetase; AsnRS; Asparaginyl-tRNA synthetase 1; EC 6.1.1.22 from Homo sapiens (Human) (see 6 papers)
O43776 asparagine-tRNA ligase (EC 6.1.1.22) from Homo sapiens (see 2 papers)
    29% identity, 95% coverage of query (166 bits)

8tc8A / O43776 Human asparaginyl-tRNA synthetase bound to adenosine 5'-sulfamate (see paper)
    29% identity, 98% coverage of query (165 bits)

SYDND_METTH / O26328 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) (see paper)
O26328 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Methanothermobacter thermautotrophicus (see paper)
    30% identity, 98% coverage of query (163 bits)

SYDND_HALSA / O07683 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium) (see paper)
O07683 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Halobacterium salinarum (see paper)
    28% identity, 100% coverage of query (162 bits)

3m4pA / C4LWW8 Entamoeba histolytica asparaginyl-tRNA synthetase (asnrs) in complex with asparaginyl-adenylate
    28% identity, 95% coverage of query (157 bits)

SYNC_BRUMA / P10723 Asparagine--tRNA ligase, cytoplasmic; Asparaginyl-tRNA synthetase; AsnRS; Potentially protective 63 kDa antigen; EC 6.1.1.22 from Brugia malayi (Filarial nematode worm) (see paper)
P10723 asparagine-tRNA ligase (EC 6.1.1.22) from Brugia malayi (see paper)
    29% identity, 95% coverage of query (156 bits)

SYNC_SCHPO / O94567 Probable asparagine--tRNA ligase, cytoplasmic; Asparaginyl-tRNA synthetase; AsnRS; EC 6.1.1.22 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    28% identity, 95% coverage of query (155 bits)

SYDC_YEAST / P04802 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
    33% identity, 73% coverage of query (148 bits)

1asyA / P04802 Class ii aminoacyl transfer RNA synthetases: crystal structure of yeast aspartyl-tRNA synthetase complexed with tRNA asp (see paper)
    33% identity, 73% coverage of query (147 bits)

1aszA The active site of yeast aspartyl-tRNA synthetase: structural and functional aspects of the aminoacylation reaction
    33% identity, 73% coverage of query (147 bits)

SYDND_THET8 / Q5SIC2 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase 2; AspRS2; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 4 papers)
Q5SIC2 aspartate-tRNA ligase (EC 6.1.1.12) from Thermus thermophilus (see paper)
    29% identity, 96% coverage of query (145 bits)

Q8I2B1 aspartate-tRNA ligase (EC 6.1.1.12) from Plasmodium falciparum (see paper)
    28% identity, 72% coverage of query (142 bits)

C4LZN0 aspartate-tRNA ligase (EC 6.1.1.12); aspartate-tRNAAsn ligase (EC 6.1.1.23) from Entamoeba histolytica (see paper)
    33% identity, 73% coverage of query (141 bits)

SYDC2_ARATH / Q9M084 Aspartate--tRNA ligase 2, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Protein IMPAIRED IN BABA-INDUCED DISEASE IMMUNITY 1; EC 6.1.1.12 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    31% identity, 72% coverage of query (141 bits)

SYDC_HUMAN / P14868 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein; EC 6.1.1.12 from Homo sapiens (Human) (see 4 papers)
P14868 aspartate-tRNA ligase (EC 6.1.1.12) from Homo sapiens (see 2 papers)
    30% identity, 72% coverage of query (137 bits)

SYDC_MOUSE / Q922B2 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Mus musculus (Mouse) (see paper)
Q922B2 aspartate-tRNA ligase (EC 6.1.1.12) from Mus musculus (see paper)
    30% identity, 72% coverage of query (134 bits)

SYDC_RAT / P15178 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Rattus norvegicus (Rat) (see paper)
    29% identity, 72% coverage of query (132 bits)

SYDC_SCHPO / O74407 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    30% identity, 73% coverage of query (131 bits)

Q585M3 aspartate-tRNA ligase (EC 6.1.1.12) from Trypanosoma brucei (see paper)
    24% identity, 98% coverage of query (126 bits)

aspS2 / Q9RVH4 aspartyl-tRNA synthetase, non-discriminating (EC 6.1.1.23) from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) (see 2 papers)
SYDND_DEIRA / Q9RVH4 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase 2; AspRS2; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) (see 2 papers)
    25% identity, 100% coverage of query (120 bits)

erdS / Q4WKG3 aspartate--tRNA ligase/ergosteryl-3β-O-L-aspartate synthase (EC 6.1.1.23; EC 2.3.2.37) from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (see paper)
    31% identity, 71% coverage of query (118 bits)

Q38CB4 aspartate-tRNA ligase (EC 6.1.1.12) from Trypanosoma brucei (see paper)
    25% identity, 98% coverage of query (112 bits)

3kfuC / Q5SIC2 Crystal structure of the transamidosome (see paper)
    25% identity, 95% coverage of query (99.4 bits)

slgZ / CBA11568.1 asparaginyl-tRNA synthetase-like from Streptomyces lydicus (see paper)
    26% identity, 72% coverage of query (96.7 bits)

3a74A / Q9RHV9 Lysyl-tRNA synthetase from bacillus stearothermophilus complexed with diadenosine tetraphosphate (ap4a)
    25% identity, 96% coverage of query (89.4 bits)

3e9iA Lysyl-tRNA synthetase from bacillus stearothermophilus complexed with l-lysine hydroxamate-amp
    25% identity, 96% coverage of query (89.4 bits)

3e9hA Lysyl-tRNA synthetase from bacillus stearothermophilus complexed with l-lysylsulfamoyl adenosine
    25% identity, 96% coverage of query (89.4 bits)

SYK_STAAU / Q53638 Lysine--tRNA ligase; Lysyl-tRNA synthetase; LysRS; EC 6.1.1.6 from Staphylococcus aureus (see paper)
    24% identity, 97% coverage of query (86.7 bits)

Q9RHV9 lysine-tRNA ligase (EC 6.1.1.6) from Geobacillus stearothermophilus (see 3 papers)
    25% identity, 96% coverage of query (85.9 bits)

1e1tA / P0A8N5 Lysyl-tRNA synthetase (lysu) hexagonal form complexed with the lysyl_adenylate intermediate (see paper)
    24% identity, 96% coverage of query (80.9 bits)

5yzxA Crystal structure of e.Coli lysu t146d mutant
    24% identity, 96% coverage of query (80.9 bits)

1e24A Lysyl-tRNA synthetase (lysu) hexagonal form complexed with lysine and atp and mn2+
    24% identity, 96% coverage of query (80.9 bits)

1e22A Lysyl-tRNA synthetase (lysu) hexagonal form complexed with lysine and the non-hydrolysable atp analogue amp-pcp
    24% identity, 96% coverage of query (80.9 bits)

1bbuA / P0A8N3 Lysyl-tRNA synthetase (lyss) complexed with lysine (see paper)
    25% identity, 95% coverage of query (80.5 bits)

Q04LI2 lysine-tRNA ligase (EC 6.1.1.6) from Streptococcus pneumoniae (see paper)
    24% identity, 94% coverage of query (80.1 bits)

HerC / b2890 lysine—tRNA ligase, constitutive (EC 6.1.1.6) from Escherichia coli K-12 substr. MG1655 (see 53 papers)
lysS / P0A8N3 lysine—tRNA ligase (EC 6.1.1.6) from Escherichia coli (strain K12) (see 53 papers)
lysS lysine--tRNA ligase; EC 6.1.1.6 from Escherichia coli K12 (see paper)
    25% identity, 95% coverage of query (79.0 bits)

1e1oA Lysyl-tRNA synthetase (lysu) hexagonal form, complexed with lysine
    24% identity, 96% coverage of query (78.6 bits)

6wbdB / B2FQ84 Crystal structure of lysyl-tRNA synthetase from stenotrophomonas maltophilia with bound l-lysine
    24% identity, 95% coverage of query (77.4 bits)

LysU / b4129 lysine—tRNA ligase/Ap4A synthetase/Ap3A synthetase (EC 6.1.1.6) from Escherichia coli K-12 substr. MG1655 (see 70 papers)
lysU / P0A8N5 lysyl-tRNA synthetase (EC 6.1.1.6) from Escherichia coli (strain K12) (see 73 papers)
P0A8N5 lysine-tRNA ligase (EC 6.1.1.6) from Escherichia coli (see paper)
lysU / RF|NP_418553 lysyl-tRNA synthetase, heat inducible; EC 6.1.1.6 from Escherichia coli K12 (see 10 papers)
    24% identity, 96% coverage of query (76.6 bits)

P0A8N5 Lysine--tRNA ligase, heat inducible; Lysyl-tRNA synthetase; LysRS; EC 6.1.1.6 from Escherichia coli (strain K12)
    24% identity, 96% coverage of query (76.6 bits)

4rmfA Biochemical and structural characterization of mycobacterial aspartyl- tRNA synthetase asps, a promising tb drug target
    26% identity, 70% coverage of query (72.4 bits)

4o2dB / A0QWN3 Crystal structure of aspartyl-tRNA synthetase from mycobacterium smegmatis with bound aspartic acid (see paper)
    25% identity, 70% coverage of query (70.5 bits)

4o2dA / A0QWN3 Crystal structure of aspartyl-tRNA synthetase from mycobacterium smegmatis with bound aspartic acid (see paper)
    25% identity, 70% coverage of query (70.5 bits)

4ex5A / Q2SXD6 Crystal structure of lysyl-tRNA synthetase lysrs from burkholderia thailandensis bound to lysine (see paper)
    25% identity, 96% coverage of query (70.1 bits)

SYDND_PSEAE / Q51422 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    25% identity, 71% coverage of query (68.9 bits)

Q580G3 lysine-tRNA ligase (EC 6.1.1.6) from Trypanosoma brucei (see paper)
    24% identity, 94% coverage of query (68.2 bits)

4wj4A Crystal structure of non-discriminating aspartyl-tRNA synthetase from pseudomonas aeruginosa complexed with tRNA(asn) and aspartic acid
    25% identity, 70% coverage of query (67.8 bits)

4wj3M / Q51422 Crystal structure of the asparagine transamidosome from pseudomonas aeruginosa (see paper)
    25% identity, 70% coverage of query (67.4 bits)

5zh3A Crystal structure of pfkrs with inhibitor clado-6
    23% identity, 84% coverage of query (64.7 bits)

4pg3A Crystal structure of krs complexed with inhibitor
    24% identity, 84% coverage of query (63.5 bits)

5zh4A Crystal structure of pfkrs with inhibitor clado-7
    24% identity, 84% coverage of query (63.2 bits)

5zh3B Crystal structure of pfkrs with inhibitor clado-6
    23% identity, 97% coverage of query (62.4 bits)

5zh2A / W7JP72 Crystal structure of pfkrs with inhibitor clado-5 (see paper)
    23% identity, 84% coverage of query (61.2 bits)

6l3yA Crystal structure of lysyl-tRNA synthetase from plasmodium falciparum complexed with l-lysine and clado-c
    24% identity, 84% coverage of query (60.8 bits)

5zh5A Crystal structure of pfkrs with inhibitor clado-2
    24% identity, 84% coverage of query (60.1 bits)

6hcvA Crystal structure of lysyl-tRNA synthetase from plasmodium falciparum complexed with a chromone ligand
    23% identity, 84% coverage of query (60.1 bits)

8k9wA Crystal structure of plasmodium lysrs complexing with asp3026 derived lysrs inhibitor 4 (adki4)
    23% identity, 97% coverage of query (59.7 bits)

P15180 Lysine--tRNA ligase, cytoplasmic; Lysyl-tRNA synthetase; LysRS; EC 6.1.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    23% identity, 97% coverage of query (59.3 bits)

7zogA Crystal structure of cryptosporidium parvum -plasmodium falciparum mutant lysyl tRNA synthetase in complex with inhibitor
    25% identity, 88% coverage of query (58.5 bits)

7f6wA / P15180 Crystal structure of saccharomyces cerevisiae lysyl-tRNA synthetase (see paper)
    23% identity, 97% coverage of query (58.5 bits)

6bniA / Q5CR27 Crystal structure of lysyl-tRNA synthetase from cryptosporidium parvum complexed with l-lysine and adenosine
    24% identity, 89% coverage of query (58.2 bits)

5eloA Crystal structure of lysyl-tRNA synthetase from cryptosporidium parvum complexed with l-lysine and cladosporin
    24% identity, 89% coverage of query (58.2 bits)

6hcwA Crystal structure of lysyl-tRNA synthetase from cryptosporidium parvum complexed with l-lysine and a difluoro cyclohexyl chromone ligand
    24% identity, 89% coverage of query (57.8 bits)

6c86A Crystal structure of lysyl-tRNA synthetase from cryptosporidium parvum complexed with l-lysylsulfamoyl adenosine
    24% identity, 89% coverage of query (57.8 bits)

7qi8A Crystal structure of lysyl-tRNA synthetase from mycobacterium tuberculosis complexed with l-lysine and inhibitor
    23% identity, 96% coverage of query (56.6 bits)

7qh8A / P9WFU9 Crystal structure of lysyl-tRNA synthetase from mycobacterium tuberculosis complexed with l-lysine (see paper)
    22% identity, 96% coverage of query (56.6 bits)

7qhnA Crystal structure of lysyl-tRNA synthetase from mycobacterium tuberculosis complexed with l-lysine and an inhibitor
    23% identity, 96% coverage of query (55.1 bits)

6aqgC / A0PV47 Crystal structure of lysyl-tRNA synthetase from mycobacterium ulcerans complexed with l-lysine and cladosporin
    21% identity, 98% coverage of query (43.5 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory