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Searching for up to 100 curated homologs for WP_018126152.1 NCBI__GCF_000375485.1:WP_018126152.1 (611 a.a.)

Found high-coverage hits (≥70%) to 38 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

DvMF_2038 aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) from Desulfovibrio vulgaris Miyazaki F
    64% identity, 99% coverage of query (826 bits)

4wj3M / Q51422 Crystal structure of the asparagine transamidosome from pseudomonas aeruginosa (see paper)
    56% identity, 95% coverage of query (647 bits)

SYDND_PSEAE / Q51422 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    56% identity, 95% coverage of query (646 bits)

4wj4A Crystal structure of non-discriminating aspartyl-tRNA synthetase from pseudomonas aeruginosa complexed with tRNA(asn) and aspartic acid
    56% identity, 95% coverage of query (646 bits)

O32038 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Bacillus subtilis (see paper)
    54% identity, 95% coverage of query (639 bits)

Q6MI59 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Bdellovibrio bacteriovorus (see paper)
    52% identity, 96% coverage of query (621 bits)

Q9KDG1 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Alkalihalobacillus halodurans (see paper)
    52% identity, 96% coverage of query (614 bits)

Tls / b1866 aspartate—tRNA ligase (EC 6.1.1.12) from Escherichia coli K-12 substr. MG1655 (see 24 papers)
aspS / P21889 aspartate—tRNA ligase (EC 6.1.1.12; EC 6.1.1.23) from Escherichia coli (strain K12) (see 26 papers)
SYD_ECOLI / P21889 Aspartate--tRNA ligase; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Escherichia coli (strain K12) (see paper)
    53% identity, 95% coverage of query (605 bits)

1c0aA / P21889 Crystal structure of the e. Coli aspartyl-tRNA synthetase : trnaasp : aspartyl-adenylate complex (see paper)
    53% identity, 95% coverage of query (603 bits)

Synpcc7942_1313 aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) from Synechococcus elongatus PCC 7942
    50% identity, 94% coverage of query (589 bits)

SYDND_HELPY / P56459 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
P56459 aspartate-tRNA ligase (EC 6.1.1.12) from Helicobacter pylori (see paper)
    50% identity, 95% coverage of query (574 bits)

aspS1 / Q9RUN7 aspartyl-tRNA synthetase subunit (EC 6.1.1.23) from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) (see 2 papers)
SYD_DEIRA / Q9RUN7 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase 1; AspRS1; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) (see paper)
    51% identity, 92% coverage of query (566 bits)

6sjcB / Q5SKD2 Structure of t. Thermophilus asprs in complex with 5'-o-(n-(l- aspartyl)-sulfamoyl)adenosine (see paper)
    52% identity, 95% coverage of query (562 bits)

SYD_THETH / P36419 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase; AspRS; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 from Thermus thermophilus (see 2 papers)
SYD_THET8 / Q5SKD2 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase 1; AspRS1; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 2 papers)
P36419 aspartate-tRNA ligase (EC 6.1.1.12) from Thermus thermophilus (see 2 papers)
    52% identity, 94% coverage of query (561 bits)

1g51B Aspartyl tRNA synthetase from thermus thermophilus at 2.4 a resolution
    52% identity, 94% coverage of query (561 bits)

1g51A Aspartyl tRNA synthetase from thermus thermophilus at 2.4 a resolution
    52% identity, 94% coverage of query (561 bits)

1efwA Crystal structure of aspartyl-tRNA synthetase from thermus thermophilus complexed to trnaasp from escherichia coli
    52% identity, 94% coverage of query (561 bits)

6hhxA Structure of t. Thermophilus asprs in complex with 5'-o-(n-(l- aspartyl)-sulfamoyl)cytidine
    52% identity, 95% coverage of query (553 bits)

6hhwA Structure of t. Thermophilus asprs in complex with 5'-o-(n-(l- aspartyl)-sulfamoyl)uridine
    52% identity, 95% coverage of query (553 bits)

6hhvA Structure of t. Thermophilus asprs in complex with 5'-o-(n-(l- aspartyl)-sulfamoyl)n3-methyluridine
    52% identity, 95% coverage of query (553 bits)

4rmfA Biochemical and structural characterization of mycobacterial aspartyl- tRNA synthetase asps, a promising tb drug target
    49% identity, 94% coverage of query (552 bits)

7ap4A Thermus thermophilus aspartyl-tRNA synthetase in complex with compound asps7hmdda
    52% identity, 95% coverage of query (548 bits)

4o2dA / A0QWN3 Crystal structure of aspartyl-tRNA synthetase from mycobacterium smegmatis with bound aspartic acid (see paper)
    49% identity, 94% coverage of query (545 bits)

SYDND_CHLTR / O84546 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Chlamydia trachomatis serovar D (strain ATCC VR-885 / DSM 19411 / UW-3/Cx) (see paper)
    49% identity, 95% coverage of query (545 bits)

P9WFW3 aspartate-tRNA ligase (EC 6.1.1.12) from Mycobacterium tuberculosis (see paper)
    47% identity, 96% coverage of query (542 bits)

SYDM_ARATH / F4JJT9 Aspartate--tRNA ligase, chloroplastic/mitochondrial; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    47% identity, 95% coverage of query (541 bits)

5w25A / P9WFW3 Crystal structure of aspartyl-tRNA synthetase from mycobacterium tuberculosis complexed with l-aspartic acid
    47% identity, 94% coverage of query (537 bits)

4o2dB / A0QWN3 Crystal structure of aspartyl-tRNA synthetase from mycobacterium smegmatis with bound aspartic acid (see paper)
    44% identity, 94% coverage of query (465 bits)

CCNA_01969 aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) from Caulobacter crescentus NA1000
    42% identity, 94% coverage of query (453 bits)

PGA1_c24530 aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) from Phaeobacter inhibens BS107
    41% identity, 95% coverage of query (446 bits)

Dshi_2633 aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) from Dinoroseobacter shibae DFL-12
    41% identity, 95% coverage of query (442 bits)

SYDM_HUMAN / Q6PI48 Aspartate--tRNA ligase, mitochondrial; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Homo sapiens (Human) (see 4 papers)
Q6PI48 aspartate-tRNA ligase (EC 6.1.1.12) from Homo sapiens (see 3 papers)
    40% identity, 96% coverage of query (426 bits)

SYDM_SCHPO / O94242 Aspartate--tRNA ligase, mitochondrial; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    33% identity, 94% coverage of query (302 bits)

SYDM_YEAST / P15179 Aspartate--tRNA ligase, mitochondrial; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
    35% identity, 94% coverage of query (285 bits)

Build an alignment

Build an alignment for WP_018126152.1 and 34 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

C4M7X2 lysine-tRNA ligase (EC 6.1.1.6) from Entamoeba histolytica (see paper)
    26% identity, 88% coverage of query (117 bits)

P15180 Lysine--tRNA ligase, cytoplasmic; Lysyl-tRNA synthetase; LysRS; EC 6.1.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    23% identity, 90% coverage of query (93.6 bits)

7f6wA / P15180 Crystal structure of saccharomyces cerevisiae lysyl-tRNA synthetase (see paper)
    22% identity, 87% coverage of query (92.4 bits)

SYK_MOUSE / Q99MN1 Lysine--tRNA ligase; Lysyl-tRNA synthetase; LysRS; EC 2.7.7.-; EC 6.1.1.6 from Mus musculus (Mouse) (see paper)
Q99MN1 lysine-tRNA ligase (EC 6.1.1.6) from Mus musculus (see paper)
    24% identity, 90% coverage of query (90.5 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory