Sites on a Tree

 

Searching for up to 100 curated homologs for WP_020174371.1 NCBI__GCF_000385335.1:WP_020174371.1 (494 a.a.)

Found high-coverage hits (≥70%) to 14 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

MCTP_RHIJ3 / Q1M7A2 Monocarboxylate transport permease protein from Rhizobium johnstonii (strain DSM 114642 / LMG 32736 / 3841) (Rhizobium leguminosarum bv. viciae) (see paper)
    78% identity, 94% coverage of query (686 bits)

TC 2.A.21.4.1 / Q8VM88 The monocarboxylate uptake (H+ symport?) permease, MctP (transports lactate (Km = 4.4 μM), pyruvate (Km = 3.8), propionate, butyrate (butanoic acid), α-hydroxybutyrate, L- and D-alanine (Km = 0.5 mM), and possibly cysteine and histidine) from Rhizobium leguminosarum (biovar viciae) (see paper)
mctP / CAD19128.1 monocarboxylic acid permease from Rhizobium leguminosarum bv. viciae (see paper)
    78% identity, 94% coverage of query (683 bits)

BPHYT_RS22245 transporter for D-alanine, D-lactate, and pyruvate (MctP) from Burkholderia phytofirmans PsJN
    66% identity, 93% coverage of query (568 bits)

Build an alignment

Build an alignment for WP_020174371.1 and 3 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

YjcG / b4067 acetate/glycolate:cation symporter from Escherichia coli K-12 substr. MG1655 (see 2 papers)
actP / P32705 acetate/glycolate:cation symporter from Escherichia coli (strain K12) (see 5 papers)
ACTP_ECOLI / P32705 Cation/acetate symporter ActP; Acetate permease; Acetate transporter ActP from Escherichia coli (strain K12) (see 2 papers)
TC 2.A.21.7.2 / P32705 Acetate/glyoxylate permease, ActP from Escherichia coli (see 6 papers)
    22% identity, 86% coverage of query (55.1 bits)

SC5A7_CAEEL / O02228 High-affinity choline transporter 1 from Caenorhabditis elegans (see paper)
    26% identity, 87% coverage of query (54.7 bits)

PS417_08865 large component of pyruvate transporter (actP-like) from Pseudomonas simiae WCS417
    23% identity, 86% coverage of query (54.3 bits)

PUTP_STAA8 / Q2FWY7 Sodium/proline symporter; Proline permease from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see 3 papers)
TC 2.A.21.2.2 / Q7A4Q7 Sodium/proline symporter (Proline permease) from Staphylococcus aureus (strain N315) (see paper)
    24% identity, 96% coverage of query (52.0 bits)

Pf1N1B4_3673 large component of pyruvate transporter (actP-like) from Pseudomonas fluorescens FW300-N1B4
    23% identity, 86% coverage of query (51.6 bits)

PutC / b1015 proline:Na+ symporter from Escherichia coli K-12 substr. MG1655 (see 7 papers)
putP / P07117 proline:Na+ symporter from Escherichia coli (strain K12) (see 12 papers)
PUTP_ECOLI / P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 9 papers)
TC 2.A.21.2.1 / P07117 Proline:Na+ symporter, PutP from Escherichia coli (see 12 papers)
    22% identity, 73% coverage of query (50.8 bits)

TC 2.A.21.2.3 / Q9I5F5 L-proline uptake porter, PutP from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    21% identity, 77% coverage of query (50.1 bits)

OPUE_BACSU / O06493 Osmoregulated proline transporter OpuE; Osmoprotectant uptake; Proline uptake system from Bacillus subtilis (strain 168) (see 2 papers)
TC 2.A.21.2.5 / M1TZ79 Proline uptake porter, OpuE (YerK) from Bacillus subtilis subsp. subtilis 6051-HGW
opuE / AAB72182.1 OpuE from Bacillus subtilis (see paper)
    24% identity, 75% coverage of query (49.7 bits)

Pf6N2E2_5849 large component of pyruvate transporter (actP-like) from Pseudomonas fluorescens FW300-N2E2
    23% identity, 86% coverage of query (48.5 bits)

putP / AAA79365.1 proline permease homolog from Staphylococcus aureus (see paper)
    23% identity, 96% coverage of query (47.8 bits)

PUTP_SALTY / P10502 Sodium/proline symporter; Proline permease from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
    21% identity, 75% coverage of query (47.0 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory