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Searching for up to 100 curated homologs for WP_024771447.1 NCBI__GCF_000520995.1:WP_024771447.1 (445 a.a.)

Found high-coverage hits (≥70%) to 70 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Sama_1319 symporter for L-glutamate, L-glutamine, and L-aspartate from Shewanella amazonensis SB2B
    34% identity, 90% coverage of query (242 bits)

Psest_4075 symporter for L-glutamate, L-glutamine, and L-proline from Pseudomonas stutzeri RCH2
    35% identity, 91% coverage of query (225 bits)

GLTT_BACSU / O07605 Proton/sodium-glutamate symport protein; Glutamate-aspartate carrier protein from Bacillus subtilis (strain 168) (see 2 papers)
    33% identity, 93% coverage of query (220 bits)

5e9sA / Q5JID0 Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
    35% identity, 94% coverage of query (218 bits)

GLT_PYRHO / O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 9 papers)
TC 2.A.23.1.5 / O59010 Archaeal aspartate transporter, Gltph (GltPh) (3-D structure known; 3V8F and 3V8G) (Boudker et al., 2007; Yernool et al., 2004). Cotransports aspartate with 2 Na+ (Ryan et al., 2009) or 3 Na+ (Groeneveld and Slotboom, 2010) or 1Na+ plus 1 H+ plus 1 K+ from Pyrococcus horikoshii (see 2 papers)
    34% identity, 91% coverage of query (218 bits)

2nwwA Crystal structure of gltph in complex with tboa
    34% identity, 91% coverage of query (218 bits)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor
    35% identity, 94% coverage of query (218 bits)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis)
    35% identity, 93% coverage of query (218 bits)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound
    35% identity, 93% coverage of query (218 bits)

6x15A / O59010 Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
    34% identity, 91% coverage of query (216 bits)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa
    34% identity, 91% coverage of query (215 bits)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine
    34% identity, 91% coverage of query (214 bits)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate
    34% identity, 91% coverage of query (214 bits)

6bauA Crystal structure of gltph r397c in complex with l-cysteine
    34% identity, 91% coverage of query (214 bits)

TC 2.A.23.2.11 / B0W0K4 EAAT homologue, a glutamate/aspartate preferring transporter of 483 aas from Culex quinquefasciatus
    31% identity, 93% coverage of query (197 bits)

Build an alignment

Build an alignment for WP_024771447.1 and 15 homologs with ≥ 30% identity

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Change minimum %identity:

Additional hits (identity < 30%)

EAA3_RABIT / P31597 Excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1 from Oryctolagus cuniculus (Rabbit) (see paper)
    29% identity, 97% coverage of query (196 bits)

O35874 Neutral amino acid transporter A; Alanine/serine/cysteine/threonine transporter 1; ASCT-1; Solute carrier family 1 member 4 from Mus musculus (Mouse)
    31% identity, 92% coverage of query (195 bits)

GLTT_BACCA / P24944 Proton/sodium-glutamate symport protein; Glutamate-aspartate carrier protein from Bacillus caldotenax (see 2 papers)
gltT / GI|143002 proton/sodium-glutamate symport protein GltT from Bacillus caldotenax (see 4 papers)
    32% identity, 86% coverage of query (192 bits)

AAAT_RABIT / O19105 Neutral amino acid transporter B(0); ATB(0); Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 member 5 from Oryctolagus cuniculus (Rabbit) (see paper)
    32% identity, 80% coverage of query (192 bits)

AAAT_MOUSE / P51912 Neutral amino acid transporter B(0); ATB(0); ASC-like Na(+)-dependent neutral amino acid transporter ASCT2; Insulin-activated amino acid transporter; Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 member 5 from Mus musculus (Mouse) (see 4 papers)
TC 2.A.23.3.2 / P51912 Insulin-activated, Na+-dependet amino acid (serine, alanine, glutamate, glutamine and other neutral amino acids):amino acid antiporter (Ndaru et al. 2019). Also transports homocysteine from Mus musculus (Mouse) (see 2 papers)
Slc1a5 neutral amino acid transporter B(0) from Mus musculus (see paper)
    30% identity, 95% coverage of query (191 bits)

SATT_HUMAN / P43007 Neutral amino acid transporter A; Alanine/serine/cysteine/threonine transporter 1; ASCT-1; Solute carrier family 1 member 4 from Homo sapiens (Human) (see 7 papers)
TC 2.A.23.3.1 / P43007 Neutral amino acid (alanine, serine, cysteine, threonine):Na+ symporter. Also transports homocysteine (Jiang et al., 2007). AscT1 is the Syncytin-1 (Q9UQF0) receptor. Syncytin-1, of 538 aas with 4-7 TMSs, is a viral fusion protein and is involved in the development of multiple sclerosis (Antony et al. 2007). Mutation causes nuerological problems including global developmental delay, severe progressive microcephaly, seizures, spasticity and thin corpus callosum (CC) from Homo sapiens (Human) (see 9 papers)
SLC1A4 / RF|NP_003029.2 neutral amino acid transporter A from Homo sapiens (see paper)
    32% identity, 81% coverage of query (191 bits)

GLTT_GEOSE / P24943 Proton/sodium-glutamate symport protein; Glutamate-aspartate carrier protein from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 3 papers)
TC 2.A.23.1.2 / P24943 Glutamate/aspartate:Na+ + H+ symporter from Bacillus stearothermophilus (see 4 papers)
gltT / GI|143000 proton/sodium-glutamate symport protein GltT from Geobacillus stearothermophilus (see 4 papers)
    32% identity, 86% coverage of query (190 bits)

6bmiA Crystal structure of gltph r397c in complex with l-serine
    32% identity, 91% coverage of query (190 bits)

TC 2.A.23.2.10 / Q10901 Excitatory amino acid transporter (Sodium-dependent glutamate/aspartate transporter), Gkt-1 of 503 aas and 9 - 11 TMSs from Caenorhabditis elegans (see 5 papers)
    28% identity, 95% coverage of query (189 bits)

Q10901 Excitatory amino acid transporter; Sodium-dependent glutamate/ aspartate transporter from Caenorhabditis elegans
    28% identity, 95% coverage of query (189 bits)

7xr4A / P43004 Structure of human excitatory amino acid transporter 2 (eaat2) in complex with glutamate (see paper)
    32% identity, 91% coverage of query (188 bits)

AAAT_BOVIN / Q95JC7 Neutral amino acid transporter B(0); ATB(0); Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 member 5 from Bos taurus (Bovine) (see paper)
    31% identity, 85% coverage of query (188 bits)

EAA3_MOUSE / P51906 Excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1 from Mus musculus (Mouse) (see 6 papers)
    30% identity, 87% coverage of query (188 bits)

7awmA / P43003,Q15758 Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
    32% identity, 81% coverage of query (187 bits)

EAA3_RAT / P51907 Excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1 from Rattus norvegicus (Rat) (see 4 papers)
    30% identity, 87% coverage of query (187 bits)

7xr6A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613
    32% identity, 91% coverage of query (186 bits)

EAA5_HUMAN / O00341 Excitatory amino acid transporter 5; Retinal glutamate transporter; Solute carrier family 1 member 7 from Homo sapiens (Human) (see paper)
TC 2.A.23.2.5 / O00341 solute carrier family 1 (glutamate transporter), member 7 from Homo sapiens (see 4 papers)
    29% identity, 87% coverage of query (185 bits)

GltP / b4077 glutamate/aspartate : H+ symporter GltP from Escherichia coli K-12 substr. MG1655 (see paper)
gltP / P21345 glutamate/aspartate : H+ symporter GltP from Escherichia coli (strain K12) (see 5 papers)
GLTP_ECOLI / P21345 Proton/glutamate-aspartate symporter; Glutamate-aspartate carrier protein; Proton-glutamate-aspartate transport protein from Escherichia coli (strain K12) (see 5 papers)
TC 2.A.23.1.1 / P21345 Glutamate/aspartate:H+ symporter, GltP or GltT; has 8 TMSs with 2 re-entrant loops as for GltPh (TC# 2.A.23.1.5) from Escherichia coli (see 6 papers)
    29% identity, 96% coverage of query (184 bits)

EAA2_RAT / P31596 Excitatory amino acid transporter 2; GLT-1; Sodium-dependent glutamate/aspartate transporter 2; GLUT-R; Solute carrier family 1 member 2 from Rattus norvegicus (Rat) (see 3 papers)
TC 2.A.23.2.2 / P31596 Glutamate/aspartate:Na+ symporter, GLT1; GLUT-R; EAAT2. Interacts directly with the Na+, K+-ATPase (TC #3.A.3.1) (Rose et al., 2009). Cotransports glutamic acid with three Na+ followed by countertransport of K+ (Teichman et al., 2009). The C-terminal 74aa domain regulates transport activity (Leinenweber et al., 2011). Hippocampal glutamate transporter 1 (GLT-1) levels parallel memory training (Heo et al., 2011). GLT-1 is regulated by MAGI-1 from Rattus norvegicus (Rat) (see 5 papers)
    29% identity, 97% coverage of query (184 bits)

8cv2A / P43005 Human excitatory amino acid transporter 3 (eaat3) in an outward facing sodium-bound state (see paper)
    29% identity, 93% coverage of query (184 bits)

EAA2_MOUSE / P43006 Excitatory amino acid transporter 2; GLT-1; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2 from Mus musculus (Mouse) (see 6 papers)
    30% identity, 91% coverage of query (182 bits)

TC 2.A.23.2.12 / B0W0K5 Dicarboxylic acid over dicarboxylic amino acid from Culex quinquefasciatus
    32% identity, 94% coverage of query (181 bits)

8cuaA Human excitatory amino acid transporter 3 (eaat3) with bound potassium in an intermediate outward facing state
    30% identity, 94% coverage of query (181 bits)

6x2zA Heaat3-ofs-asp
    31% identity, 93% coverage of query (180 bits)

EAA2_HUMAN / P43004 Excitatory amino acid transporter 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2 from Homo sapiens (Human) (see 9 papers)
TC 2.A.23.2.7 / P43004 Excitatory amino acid transporter 2, EAAT2 (Glutamate/aspartate transporter II) (Sodium-dependent glutamate/aspartate transporter 2) (Solute carrier family 1 member 2) from Homo sapiens (see 9 papers)
    29% identity, 97% coverage of query (179 bits)

7nsgA Structure of human excitatory amino acid transporter 3 (eaat3) in complex with hip-b
    31% identity, 95% coverage of query (179 bits)

6s3qA Structure of human excitatory amino acid transporter 3 (eaat3) in complex with tfb-tboa
    31% identity, 95% coverage of query (179 bits)

8ctcA Human excitatory amino acid transporter 3 (eaat3) with bound glutamate in an intermediate outward facing state
    31% identity, 91% coverage of query (179 bits)

5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101
    32% identity, 81% coverage of query (178 bits)

EAA5_MOUSE / Q8JZR4 Excitatory amino acid transporter 5; Solute carrier family 1 member 7 from Mus musculus (Mouse) (see 2 papers)
    28% identity, 87% coverage of query (177 bits)

8ouiC Complex of asct2 with suppressyn (see paper)
    33% identity, 81% coverage of query (177 bits)

8qrrB / Q15758 Asct2 protomer in lipid nanodiscs with bound glutamine and na+ ions in the outward-facing state (ofs.3) (see paper)
    33% identity, 83% coverage of query (175 bits)

EAA3_HUMAN / P43005 Excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1 from Homo sapiens (Human) (see 9 papers)
TC 2.A.23.2.3 / P43005 Glutamate/aspartate/cysteine:Na+ symporter, EAAC1; EAAT3, SLC1A1 (Li+ can replace Na+; EAAC1 also mediates glutamate-independent anion conductance.) Cotransports glutamic acid with three Na+ followed by countertransport of K+(Teichman et al., 2009). The 50 residue 4B-4C loop (following TMS4) binds Na+ (Koch et al., 2007). (The dicarboxylic aminoaciduria protein in humans; NP_004161; Bröer, 2008a; 2008b). Neutralizing aspartate 83 modifies substrate translocation from Homo sapiens (Human) (see 6 papers)
    29% identity, 97% coverage of query (175 bits)

8oudA Structure of the human neutral amino acid transporter asct2 in complex with nanobody 469
    33% identity, 83% coverage of query (174 bits)

7vr7A Inward-facing structure of human eaat2 in the way213613-bound state
    32% identity, 88% coverage of query (174 bits)

EAA1_AMBTI / O57321 Excitatory amino acid transporter 1; SEAAT1; Sodium-dependent glutamate/aspartate transporter; GLAST from Ambystoma tigrinum (Eastern tiger salamander) (see 2 papers)
    29% identity, 88% coverage of query (172 bits)

RR42_RS03990 aspartate:proton symporter Glt from Cupriavidus basilensis FW507-4G11
    27% identity, 85% coverage of query (168 bits)

GLTP_BACSU / P39817 Proton/glutamate-aspartate symporter; Proton/glutamate symport protein from Bacillus subtilis (strain 168) (see paper)
GltP / AAA82878.1 proton/glutamate symport protein from Bacillus subtilis (see paper)
    28% identity, 86% coverage of query (167 bits)

EAA1_RAT / P24942 Excitatory amino acid transporter 1; Glial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 1; GLAST; GLAST-1; Solute carrier family 1 member 3 from Rattus norvegicus (Rat) (see 4 papers)
TC 2.A.23.2.1 / P24942 Glutamate/aspartate:Na+ symporter, GLAST or EAAT1, Structural rearrangements have been probed by Leighton et al., 2006). EAAT1 interacts directly with the Na+, K+-ATPase (TC #3.A.3.1) (Rose et al., 2009). CEAT1 couples glutamate uptake to the symport of 3 Na+ and 1 H+ followed by the antiport of 1 K+. It can function as an uncoupled anion, water and/or urea channel (Vandenberg et al., 2011). Large collective motions regulate the functional properties of EAAT1 trimers from Rattus norvegicus (Rat) (see 5 papers)
Slc1a3 / RF|NP_062098.1 excitatory amino acid transporter 1 from Rattus norvegicus (see paper)
    30% identity, 88% coverage of query (166 bits)

EAA1_MOUSE / P56564 Excitatory amino acid transporter 1; Glial high affinity glutamate transporter; High-affinity neuronal glutamate transporter; GluT-1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Mus musculus (Mouse) (see 6 papers)
    29% identity, 88% coverage of query (165 bits)

TC 2.A.23.1.9 / Q1J1H5 Fumarate:H+ symporter of 442 aas and 14 established TMSs, DctA. Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from Deinococcus geothermalis (strain DSM 11300)
    29% identity, 91% coverage of query (165 bits)

AO356_01905 L-aspartate transporter from Pseudomonas fluorescens FW300-N2C3
    29% identity, 92% coverage of query (165 bits)

Q21353 Putative sodium-dependent excitatory amino acid transporter glt-3 from Caenorhabditis elegans
    30% identity, 84% coverage of query (165 bits)

EAA1_HUMAN / P43003 Excitatory amino acid transporter 1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Homo sapiens (Human) (see 8 papers)
TC 2.A.23.2.6 / P43003 Excitatory amino acid transporter 1 (EAAT1) (Sodium-dependent glutamate/aspartate transporter 1) (GLAST-1) (Solute carrier family 1 member 3) from Homo sapiens (see 9 papers)
    29% identity, 83% coverage of query (164 bits)

EAA1_BOVIN / P46411 Excitatory amino acid transporter 1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Bos taurus (Bovine) (see paper)
    29% identity, 83% coverage of query (162 bits)

AO356_18980 sodium:C4-dicarboxylate symporter (dctA) from Pseudomonas fluorescens FW300-N2C3
    28% identity, 91% coverage of query (159 bits)

DCTA2_PSEAE / Q9I4F5 C4-dicarboxylate transport protein 2 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    28% identity, 82% coverage of query (158 bits)

TC 2.A.23.1.10 / Q848I3 Organic acid uptake porter, DctA of 444 aas and 8 - 10 putative TMSs from Pseudomonas chlororaphis
    29% identity, 82% coverage of query (155 bits)

b3528 C4 dicarboxylate/orotate:H+ symporter (DctA) from Escherichia coli BW25113
DctA / b3528 C4 dicarboxylate/orotate:H+ symporter from Escherichia coli K-12 substr. MG1655 (see 13 papers)
dctA / P0A830 C4 dicarboxylate/orotate:H+ symporter from Escherichia coli (strain K12) (see 23 papers)
DCTA_ECOLI / P0A830 Aerobic C4-dicarboxylate transport protein from Escherichia coli (strain K12) (see 2 papers)
TC 2.A.23.1.7 / P0A830 Aerobic dicarboxylate transporter, DctA. Interacts with the DcuS sensor kinase from Escherichia coli (strain K12) (see 6 papers)
dctA / GB|AAC76553.1 aerobic C4-dicarboxylate transport protein from Escherichia coli (see 5 papers)
    29% identity, 84% coverage of query (155 bits)

TC 2.A.23.1.6 / P96603 The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up from Bacillus subtilis (see 2 papers)
    30% identity, 82% coverage of query (154 bits)

PfGW456L13_2016 C4-dicarboxylate transporter from Pseudomonas fluorescens GW456-L13
    29% identity, 82% coverage of query (154 bits)

PS417_21500 C4 dicarboxylate transporter DctA from Pseudomonas simiae WCS417
    27% identity, 85% coverage of query (152 bits)

CH_124149 excitatory amino acid transporter 2 from Magnaporthe grisea 70-15 (see paper)
    29% identity, 84% coverage of query (151 bits)

BWI76_RS26760 C4-dicarboxylate transport protein DctA from Klebsiella michiganensis M5al
    28% identity, 86% coverage of query (149 bits)

TC 2.A.23.1.3 / Q01857 C4-dicarboxylate transporter (substrates: fumarate, D- and L-malate, succinate, succinamide, orotate, iticonate, mesaconate) from Rhizobium leguminosarum
    28% identity, 86% coverage of query (133 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory