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Searching for up to 100 curated homologs for WP_028990604.1 NCBI__GCF_000423825.1:WP_028990604.1 (645 a.a.)

Found high-coverage hits (≥70%) to 50 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

ACON2_YEAST / P39533 Homocitrate dehydratase, mitochondrial; Aconitase 2; EC 4.2.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 5 papers)
    35% identity, 99% coverage of query (363 bits)

Q8RP87 aconitate hydratase (EC 4.2.1.3) from Bacteroides fragilis (see paper)
    36% identity, 98% coverage of query (361 bits)

Aco / O74699 aconitase from Aspergillus terreus (see paper)
    36% identity, 98% coverage of query (359 bits)

ACON2_SCHPO / Q9P7D4 Aconitase-ribosomal protein bL21m fusion protein; EC 4.2.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
Q9P7D4 aconitate hydratase (EC 4.2.1.3) from Schizosaccharomyces pombe (see paper)
    35% identity, 98% coverage of query (359 bits)

ACO1 aconitate hydratase; EC 4.2.1.3 from Candida albicans (see 2 papers)
    36% identity, 99% coverage of query (357 bits)

ACON_ASPFU / Q4WLN1 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; Homocitrate dehydratase; EC 4.2.1.3; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
    36% identity, 98% coverage of query (355 bits)

ACON_SCHPO / O13966 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    35% identity, 98% coverage of query (352 bits)

Q99KI0 aconitate hydratase (EC 4.2.1.3) from Mus musculus (see paper)
    36% identity, 98% coverage of query (350 bits)

ACON_EMENI / C8VG90 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; Homocitrate dehydratase; EC 4.2.1.3; EC 4.2.1.- from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
    35% identity, 98% coverage of query (350 bits)

ACON_YEAST / P19414 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 8 papers)
P19414 aconitate hydratase (EC 4.2.1.3) from Saccharomyces cerevisiae (see 2 papers)
    36% identity, 91% coverage of query (349 bits)

ACON_RAT / Q9ER34 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Rattus norvegicus (Rat) (see paper)
Q9ER34 aconitate hydratase (EC 4.2.1.3) from Rattus norvegicus (see 3 papers)
    36% identity, 98% coverage of query (347 bits)

ACON_BOVIN / P20004 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Bos taurus (Bovine) (see paper)
    35% identity, 98% coverage of query (345 bits)

ACO2 / Q99798 mitochondrial aconitate hydratase from Homo sapiens (see 7 papers)
ACON_HUMAN / Q99798 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Homo sapiens (Human) (see 2 papers)
Q99798 aconitate hydratase (EC 4.2.1.3) from Homo sapiens (see paper)
    36% identity, 98% coverage of query (345 bits)

8acnA Crystal structures of aconitase with isocitrate and nitroisocitrate bound
    36% identity, 98% coverage of query (345 bits)

1fghA Complex with 4-hydroxy-trans-aconitate
    36% identity, 98% coverage of query (345 bits)

1amjA Steric and conformational features of the aconitase mechanism
    36% identity, 98% coverage of query (345 bits)

1amiA Steric and conformational features of the aconitase mechanism
    36% identity, 98% coverage of query (345 bits)

1acoA Crystal structure of aconitase with transaconitate bound
    36% identity, 98% coverage of query (345 bits)

5acnA / P16276 Structure of activated aconitase. Formation of the (4fe-4s) cluster in the crystal (see paper)
    36% identity, 98% coverage of query (344 bits)

1nisA Crystal structure of aconitase with trans-aconitate and nitrocitrate bound
    36% identity, 98% coverage of query (344 bits)

ACON_PIG / P16276 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Sus scrofa (Pig) (see 4 papers)
    36% identity, 98% coverage of query (343 bits)

ACON2_EMENI / Q5B6D6 Putative aconitate hydratase, mitochondrial; Aconitase 2; EC 4.2.1.- from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
    35% identity, 99% coverage of query (342 bits)

1b0kA S642a:fluorocitrate complex of aconitase
    36% identity, 98% coverage of query (342 bits)

ACON2_ASPFU / Q4WJ90 Putative aconitate hydratase, mitochondrial; Aconitase 2; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
    37% identity, 91% coverage of query (341 bits)

ACON_CAEEL / P34455 Probable aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Caenorhabditis elegans (see paper)
    34% identity, 98% coverage of query (330 bits)

Build an alignment

Build an alignment for WP_028990604.1 and 25 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

YbhJ / b0771 putative hydratase YbhJ from Escherichia coli K-12 substr. MG1655 (see 3 papers)
    28% identity, 99% coverage of query (224 bits)

J9VIU5 methanogen homoaconitase (EC 4.2.1.114) from Cryptococcus neoformans var. grubii serotype A (see paper)
    29% identity, 98% coverage of query (220 bits)

2b3xA / P21399 Structure of an orthorhombic crystal form of human cytosolic aconitase (irp1) (see paper)
    28% identity, 78% coverage of query (203 bits)

ACO1 / P21399 cytoplasmic aconitate hydratase from Homo sapiens (see 6 papers)
ACOHC_HUMAN / P21399 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Homo sapiens (Human) (see 8 papers)
P21399 aconitate hydratase (EC 4.2.1.3) from Homo sapiens (see 3 papers)
    28% identity, 78% coverage of query (203 bits)

lys2 / RF|NP_593437.1 homoaconitate hydratase Lys2; EC 4.2.1.36 from Schizosaccharomyces pombe (see 2 papers)
    27% identity, 99% coverage of query (199 bits)

B1P2N4 homoaconitate hydratase (EC 4.2.1.36) from Penicillium chrysogenum (see paper)
    30% identity, 88% coverage of query (198 bits)

ACO3M_ARATH / Q9SIB9 Aconitate hydratase 3, mitochondrial; Aconitase 3; mACO1; Citrate hydro-lyase 3; EC 4.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
Q9SIB9 aconitate hydratase (EC 4.2.1.3) from Arabidopsis thaliana (see paper)
    28% identity, 85% coverage of query (197 bits)

D3GQL0 aconitate hydratase (EC 4.2.1.3) from Citrus clementina (see paper)
    28% identity, 80% coverage of query (197 bits)

LYS4_YEAST / P49367 Homoaconitase, mitochondrial; Homoaconitate hydratase; EC 4.2.1.36 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
P49367 homoaconitate hydratase (EC 4.2.1.36) from Saccharomyces cerevisiae (see paper)
    30% identity, 89% coverage of query (196 bits)

ACO1 / Q42560 aconitase from Arabidopsis thaliana (see paper)
ACO1_ARATH / Q42560 Aconitate hydratase 1; Aconitase 1; Citrate hydro-lyase 1; EC 4.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
Q42560 aminocyclopropanecarboxylate oxidase (EC 1.14.17.4) from Arabidopsis thaliana (see paper)
    28% identity, 80% coverage of query (194 bits)

HP15_3433 Aconitate hydratase (EC 4.2.1.3) from Marinobacter adhaerens HP15
    27% identity, 89% coverage of query (194 bits)

ACNA_THET8 / Q5SMF6 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
    28% identity, 86% coverage of query (192 bits)

ACON3_ASPFU / Q4WBR0 Putative aconitate hydratase; Aconitase 3; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
    32% identity, 77% coverage of query (192 bits)

ACO2M_ARATH / Q94A28 Aconitate hydratase 2, mitochondrial; Aconitase 2; mACO2; Citrate hydro-lyase 2; EC 4.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
    29% identity, 76% coverage of query (191 bits)

ACNA_BRADU / P70920 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
    26% identity, 96% coverage of query (191 bits)

LYS4_EMENI / Q92412 Homoaconitase, mitochondrial; Homoaconitate hydratase; EC 4.2.1.36 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
Q92412 homoaconitate hydratase (EC 4.2.1.36) from Aspergillus nidulans (see paper)
    28% identity, 88% coverage of query (191 bits)

D3GQL2 aconitate hydratase (EC 4.2.1.3) from Citrus clementina (see paper)
    28% identity, 78% coverage of query (190 bits)

ACOHC_RAT / Q63270 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Rattus norvegicus (Rat) (see paper)
Q63270 aconitate hydratase (EC 4.2.1.3) from Rattus norvegicus (see paper)
    28% identity, 75% coverage of query (189 bits)

ACOHC_MOUSE / P28271 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Mus musculus (Mouse) (see paper)
P28271 aconitate hydratase (EC 4.2.1.3) from Mus musculus (see paper)
    27% identity, 85% coverage of query (188 bits)

ACOHC_BOVIN / Q0VCU1 Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Iron-responsive element-binding protein 1; IRE-BP 1; EC 4.2.1.3 from Bos taurus (Bovine) (see paper)
    27% identity, 89% coverage of query (188 bits)

citB / P09339 aconitate hydratase (bifunctional aconitase) (EC 4.2.1.99; EC 4.2.1.3) from Bacillus subtilis (strain 168) (see 21 papers)
ACNA_BACSU / P09339 Aconitate hydratase A; ACN; Aconitase; Aconitate/2-methylaconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.- from Bacillus subtilis (strain 168) (see 7 papers)
P09339 aconitate hydratase (EC 4.2.1.3) from Bacillus subtilis (see paper)
    27% identity, 95% coverage of query (187 bits)

lysF / GI|1628374 homoaconitase, mitochondrial; EC 4.2.1.36 from Emericella nidulans (see paper)
    27% identity, 88% coverage of query (187 bits)

ACON_LEGPH / P37032 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; IP210; Iron-responsive protein-like; IRP-like; Major iron-containing protein; MICP; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) (see paper)
    27% identity, 96% coverage of query (185 bits)

3snpA / Q01059 Crystal structure analysis of iron regulatory protein 1 in complex with ferritin h ire RNA (see paper)
    28% identity, 85% coverage of query (182 bits)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    28% identity, 92% coverage of query (171 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory