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Searching for up to 100 curated homologs for WP_045585058.1 NCBI__GCF_001305595.1:WP_045585058.1 (366 a.a.)

Found high-coverage hits (≥70%) to 40 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

AZOBR_RS08260 L-proline and D-alanine ABC transporter, substrate-binding component from Azospirillum brasilense Sp245
    90% identity, 100% coverage of query (663 bits)

TC 3.A.1.4.3 / Q9L3M3 BraC, component of General L- (and D-)amino acid uptake porter (transports acidic, basic, polar, semipolar and hydrophobic amino acids). The amino and carboxyl groups do not need to be α since γ-aminobutyric acid (GABA) is a substrate. The system may function with additional binding proteins since L-alanine uptake is not dependent on BraC from Rhizobium leguminosarum (biovar viciae) (see 2 papers)
braC / CAB75553.1 periplasmic binding protein from Rhizobium leguminosarum bv. viciae (see 2 papers)
    55% identity, 97% coverage of query (397 bits)

3ip5A / Q7CX36 Structure of atu2422-gaba receptor in complex with alanine (see paper)
    58% identity, 92% coverage of query (392 bits)

3ip9A Structure of atu2422-gaba receptor in complex with gaba
    58% identity, 92% coverage of query (392 bits)

3ip7A Structure of atu2422-gaba receptor in complex with valine
    58% identity, 92% coverage of query (392 bits)

3ip6A Structure of atu2422-gaba receptor in complex with proline
    58% identity, 92% coverage of query (392 bits)

3ipcA Structure of atu2422-gaba f77a mutant receptor in complex with leucine
    57% identity, 92% coverage of query (388 bits)

AABP_PSEAE / Q9HUQ0 Amino acid binding protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    51% identity, 99% coverage of query (355 bits)

LIVB3_RHIJ3 / Q1MDE9 Leu/Ile/Val-binding protein BraC3 from Rhizobium johnstonii (strain DSM 114642 / LMG 32736 / 3841) (Rhizobium leguminosarum bv. viciae) (see paper)
    44% identity, 97% coverage of query (307 bits)

BRAC_PSEAE / P21175 Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein; LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
TC 3.A.1.4.8 / P21175 Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein, component of Branched chain amino acid uptake transporter. Transports alanine from Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    44% identity, 98% coverage of query (305 bits)

4n0qB / Q8YEE8 Crystal structure of an abc transporter, substrate-binding protein from brucella melitensis 16m in complex with l-leucine using a crystal grown in a crystal former (microlytic)
    44% identity, 93% coverage of query (304 bits)

PS417_06615 ABC transporter for branched-chain amino acids, substrate-binding component from Pseudomonas simiae WCS417
    44% identity, 97% coverage of query (296 bits)

Pf6N2E2_2921 ABC transporter for L-leucine/L-phenylalanine/D-alanine, substrate-binding component LivK from Pseudomonas fluorescens FW300-N2E2
    42% identity, 97% coverage of query (283 bits)

LivJ / b3460 branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein (EC 7.4.2.2) from Escherichia coli K-12 substr. MG1655 (see 12 papers)
LivJ / P0AD96 branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein (EC 7.4.2.2) from Escherichia coli (strain K12) (see 12 papers)
TC 3.A.1.4.1 / P0AD96 Leu/Ile/Val-binding protein LivJ aka B3460 aka LIV-BP, component of Leucine; leucine/isoleucine/valine porter from Escherichia coli (see 9 papers)
    42% identity, 99% coverage of query (274 bits)

HrbC / b3458 L-leucine/L-phenylalanine ABC transporter periplasmic binding protein (EC 7.4.2.2) from Escherichia coli K-12 substr. MG1655 (see 17 papers)
livK / P04816 L-leucine/L-phenylalanine ABC transporter periplasmic binding protein (EC 7.4.2.2) from Escherichia coli (strain K12) (see 16 papers)
TC 3.A.1.4.1 / P04816 Livk aka B3458, component of Leucine; leucine/isoleucine/valine porter from Escherichia coli (see 9 papers)
livK leucine-specific-binding protein LivK from Escherichia coli K12 (see 9 papers)
    40% identity, 96% coverage of query (269 bits)

1z16A / P0AD96 Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound leucine (see paper)
    42% identity, 94% coverage of query (266 bits)

1z18A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound valine
    42% identity, 94% coverage of query (266 bits)

1z17A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound ligand isoleucine
    42% identity, 94% coverage of query (266 bits)

1usiA / P04816 L-leucine-binding protein with phenylalanine bound (see paper)
    40% identity, 92% coverage of query (258 bits)

1uskA L-leucine-binding protein with leucine bound
    40% identity, 92% coverage of query (258 bits)

HSERO_RS00870 L-proline ABC transporter, substrate-binding component from Herbaspirillum seropedicae SmR1
    35% identity, 96% coverage of query (189 bits)

Ac3H11_2396 ABC transporter for glutamine/isoleucine/leucine/phenylalanine/proline/serine/tyrosine, substrate-binding component from Acidovorax sp. GW101-3H11
    33% identity, 99% coverage of query (171 bits)

4q6bA Crystal structure of abc transporter substrate-binding protein fromdesulfitobacterium hafniense complex with leu
    31% identity, 93% coverage of query (131 bits)

4mlcA / B8FZ96 Abc transporter substrate-binding protein fromdesulfitobacterium hafniense
    30% identity, 93% coverage of query (130 bits)

TC 3.A.1.4.10 / Q8DQI1 ABC transporter substrate-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM from Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
    30% identity, 90% coverage of query (119 bits)

4gnrA / J9PBT7 1.0 angstrom resolution crystal structure of the branched-chain amino acid transporter substrate binding protein livj from streptococcus pneumoniae str. Canada mdr_19a in complex with isoleucine
    30% identity, 90% coverage of query (118 bits)

Build an alignment

Build an alignment for WP_045585058.1 and 26 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

3td9A / Q9X0L9 Crystal structure of a leucine binding protein livk (tm1135) from thermotoga maritima msb8 at 1.90 a resolution
    29% identity, 88% coverage of query (117 bits)

4q6wA / Q7VS30 Crystal structure of periplasmic binding protein type 1 from bordetella pertussis tohama i complexed with 3-hydroxy benzoic acid
    29% identity, 82% coverage of query (100 bits)

4nqrA / Q3MFZ5 The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with alanine
    23% identity, 88% coverage of query (62.8 bits)

4rdcA The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with proline
    23% identity, 88% coverage of query (62.8 bits)

4qymA The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with methionine
    23% identity, 88% coverage of query (62.8 bits)

4otzA The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with cystein
    23% identity, 88% coverage of query (62.8 bits)

4og2A The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with leucine
    23% identity, 88% coverage of query (62.8 bits)

4oatA The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with isoleucine.
    23% identity, 88% coverage of query (62.8 bits)

4nv3A The crystal structure of a solute-binding protein (n280d mutant) from anabaena variabilis atcc 29413 in complex with valine.
    23% identity, 88% coverage of query (62.8 bits)

4rv5A The crystal structure of a solute-binding protein from anabaena variabilis atcc 29413 in complex with pyruvic acid
    23% identity, 88% coverage of query (60.8 bits)

4obbA The crystal structure of a solute-binding protein from anabaena variabilis atcc 29413 in complex with (3s)-3-methyl-2-oxopentanoic acid.
    23% identity, 88% coverage of query (60.8 bits)

3i45A / Q2RWX8 Crystal structure of putative twin-arginine translocation pathway signal protein from rhodospirillum rubrum atcc 11170
    25% identity, 84% coverage of query (58.5 bits)

8wchA / Q4FMW4 Crystal structure of sar11_0655 bound to a co-purified ligand, l- pyroglutamate (see paper)
    22% identity, 83% coverage of query (55.8 bits)

8hicA / Q7V3V9 Crystal structure of urta from prochlorococcus marinus str. Mit 9313 in complex with urea and calcium (see paper)
    24% identity, 76% coverage of query (44.3 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory