Sites on a Tree

 

Searching for up to 100 curated homologs for WP_052306284.1 NCBI__GCF_000020045.1:WP_052306284.1 (584 a.a.)

Found high-coverage hits (≥70%) to 100 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

ilvB / Q0KCU4 acetohydroxyacid synthase subunit B (EC 2.2.1.6) from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (see paper)
    64% identity, 96% coverage of query (783 bits)

IlvI / b0077 acetolactate synthase / acetohydroxybutanoate synthase, catalytic subunit (EC 2.2.1.6) from Escherichia coli K-12 substr. MG1655 (see 9 papers)
ilvI / P00893 acetolactate synthase / acetohydroxybutanoate synthase, catalytic subunit (EC 2.2.1.6) from Escherichia coli (strain K12) (see 7 papers)
P00893 acetolactate synthase (subunit 2/2) (EC 2.2.1.6) from Escherichia coli (see paper)
    55% identity, 96% coverage of query (635 bits)

G0ZSE6 acetolactate synthase (EC 2.2.1.6) from Pseudomonas sp. (see paper)
    55% identity, 96% coverage of query (633 bits)

P45261 acetolactate synthase (EC 2.2.1.6) from Haemophilus influenzae (see paper)
    52% identity, 96% coverage of query (617 bits)

Q8RL88 acetolactate synthase (subunit 2/2) (EC 2.2.1.6) from Geobacillus stearothermophilus (see paper)
ilvB / AAL99356.1 acetohydroxy acid synthase large subunit from Geobacillus stearothermophilus (see paper)
    50% identity, 96% coverage of query (558 bits)

Q5SJ01 acetolactate synthase (EC 2.2.1.6); pyruvate decarboxylase (EC 4.1.1.1) from Thermus thermophilus (see paper)
    48% identity, 96% coverage of query (525 bits)

ILVB1_MYCTU / P9WG41 Acetolactate synthase large subunit IlvB1; ALS; Acetohydroxy-acid synthase large subunit; AHAS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
P9WG41 acetolactate synthase (subunit 2/2) (EC 2.2.1.6) from Mycobacterium tuberculosis (see paper)
    50% identity, 94% coverage of query (523 bits)

Q5S3P7 acetolactate synthase (EC 2.2.1.6) from Streptomyces cinnamonensis (see 2 papers)
ilvB / AAV52901.1 acetohydroxy acid synthase large subunit from Streptomyces cinnamonensis (see paper)
    46% identity, 97% coverage of query (519 bits)

P37251 acetolactate synthase (EC 2.2.1.6) from Bacillus subtilis (see paper)
    47% identity, 96% coverage of query (515 bits)

P42463 acetolactate synthase (EC 2.2.1.6) from Corynebacterium glutamicum (see paper)
    45% identity, 95% coverage of query (499 bits)

ilvB / AAB38426.1 acetolactate synthase from Mycobacterium avium (see paper)
    48% identity, 97% coverage of query (494 bits)

ilvB / AAA93098.1 acetolactate synthase from Streptomyces avermitilis (see paper)
    45% identity, 96% coverage of query (489 bits)

ilvB / O08353 acetohydroxy-acid synthase large subunit (EC 2.2.1.6) from Methanococcus aeolicus (see 2 papers)
    44% identity, 96% coverage of query (454 bits)

Q5VB49 acetolactate synthase (EC 2.2.1.6) from Helianthus annuus (see paper)
    43% identity, 96% coverage of query (447 bits)

Q5VB44 acetolactate synthase (EC 2.2.1.6) from Helianthus annuus (see paper)
    43% identity, 95% coverage of query (446 bits)

Q5VB42 acetolactate synthase (EC 2.2.1.6) from Helianthus annuus (see paper)
    41% identity, 97% coverage of query (442 bits)

ILVG_ECOLI / P0DP90 Acetolactate synthase isozyme 2 large subunit; AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit; EC 2.2.1.6 from Escherichia coli (strain K12) (see 2 papers)
ilvG / GB|CAA28573.1 acetolactate synthase isozyme 2 large subunit; EC 2.2.1.6 from Escherichia coli K12 (see 8 papers)
    44% identity, 96% coverage of query (442 bits)

1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl
    42% identity, 97% coverage of query (439 bits)

1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl
    42% identity, 97% coverage of query (439 bits)

1t9aA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl
    42% identity, 97% coverage of query (438 bits)

Q42768 acetolactate synthase (EC 2.2.1.6) from Gossypium hirsutum (see paper)
    42% identity, 95% coverage of query (437 bits)

1t9bB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron
    42% identity, 97% coverage of query (436 bits)

1n0hA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorimuron ethyl
    42% identity, 97% coverage of query (435 bits)

ILVB_ARATH / P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 16 papers)
P17597 acetolactate synthase (EC 2.2.1.6) from Arabidopsis thaliana (see 8 papers)
    41% identity, 95% coverage of query (434 bits)

6u9dB / P07342 Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
    42% identity, 97% coverage of query (434 bits)

5k2oA / P17597 Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
    41% identity, 96% coverage of query (434 bits)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron
    41% identity, 96% coverage of query (434 bits)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam
    41% identity, 96% coverage of query (434 bits)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium
    41% identity, 96% coverage of query (434 bits)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl
    41% identity, 96% coverage of query (434 bits)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin
    41% identity, 96% coverage of query (434 bits)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl
    41% identity, 96% coverage of query (434 bits)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl
    41% identity, 96% coverage of query (434 bits)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron
    41% identity, 96% coverage of query (434 bits)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl
    41% identity, 96% coverage of query (434 bits)

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl
    41% identity, 96% coverage of query (434 bits)

ILVB_YEAST / P07342 Acetolactate synthase catalytic subunit, mitochondrial; Acetohydroxy-acid synthase catalytic subunit; AHAS; ALS; EC 2.2.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
P07342 acetolactate synthase (EC 2.2.1.6) from Saccharomyces cerevisiae (see 5 papers)
    42% identity, 97% coverage of query (433 bits)

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium
    41% identity, 96% coverage of query (433 bits)

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester
    41% identity, 96% coverage of query (433 bits)

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron
    41% identity, 96% coverage of query (433 bits)

V5Q0Z4 acetolactate synthase (EC 2.2.1.6) from Myosoton aquaticum (see paper)
    42% identity, 95% coverage of query (432 bits)

Q6K2E8 acetolactate synthase (EC 2.2.1.6) from Oryza sativa (see paper)
    42% identity, 96% coverage of query (431 bits)

A5X300 acetolactate synthase (EC 2.2.1.6) from Oryza sativa Indica Group (see paper)
    42% identity, 96% coverage of query (431 bits)

5wkcA Saccharomyces cerevisiae acetohydroxyacid synthase in complex with the herbicide penoxsulam
    41% identity, 97% coverage of query (431 bits)

1t9bA / P07342 Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
    42% identity, 97% coverage of query (430 bits)

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium
    41% identity, 96% coverage of query (430 bits)

1t9dB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl
    42% identity, 97% coverage of query (430 bits)

A0A0M4NDK0 acetolactate synthase (EC 2.2.1.6) from Poa annua (see paper)
    42% identity, 96% coverage of query (429 bits)

A0A0M3TGF7 acetolactate synthase (EC 2.2.1.6) from Poa annua (see paper)
    42% identity, 96% coverage of query (429 bits)

7egvA Acetolactate synthase from trichoderma harzianum with inhibitor harzianic acid (see paper)
    42% identity, 97% coverage of query (426 bits)

K7TWQ8 acetolactate synthase (EC 2.2.1.6) from Zea mays (see paper)
    42% identity, 97% coverage of query (423 bits)

6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl
    40% identity, 97% coverage of query (422 bits)

6delA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide chlorimuron ethyl
    40% identity, 97% coverage of query (422 bits)

6denA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron ethyl
    40% identity, 97% coverage of query (422 bits)

6deoA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron methyl
    41% identity, 97% coverage of query (422 bits)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco)
    41% identity, 95% coverage of query (422 bits)

6deqA / A0A1D8PJF9 Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
    40% identity, 97% coverage of query (421 bits)

6desA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide propoxycarbazone
    40% identity, 97% coverage of query (421 bits)

6depA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide sulfometuron methyl
    40% identity, 97% coverage of query (421 bits)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco)
    41% identity, 95% coverage of query (421 bits)

6derA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide metosulam
    40% identity, 97% coverage of query (421 bits)

ILV2 acetolactate synthase; EC 2.2.1.6 from Candida albicans (see paper)
    40% identity, 97% coverage of query (419 bits)

G4MMK2 acetolactate synthase (subunit 1/2) (EC 2.2.1.6) from Pyricularia oryzae (see paper)
    40% identity, 97% coverage of query (413 bits)

CFOL_ASPCN / A0A2I2F2I5 Acetolactate synthase catalytic subunit, mitochondrial; Acetohydroxy-acid synthase catalytic subunit; AHAS; ALS; Chlorflavonin biosynthesis cluster protein L; EC 2.2.1.6 from Aspergillus candidus (see paper)
    41% identity, 96% coverage of query (412 bits)

AN4956 acetolactate synthase; EC 2.2.1.6 from Emericella nidulans (see paper)
    41% identity, 97% coverage of query (409 bits)

ilv1 / RF|NP_595382.1 acetolactate synthase catalytic subunit; EC 2.2.1.6 from Schizosaccharomyces pombe (see 2 papers)
    41% identity, 96% coverage of query (408 bits)

6dekA Crystal structure of candida albicans acetohydroxyacid synthase catalytic subunit
    39% identity, 97% coverage of query (404 bits)

Q8U2A4 acetolactate synthase (EC 2.2.1.6) from Pyrococcus furiosus (see paper)
    39% identity, 96% coverage of query (402 bits)

6vz8D / P17597 Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
    41% identity, 90% coverage of query (402 bits)

5imsA Saccharomyces cerevisiae acetohydroxyacid synthase
    41% identity, 92% coverage of query (401 bits)

6wo1A / Q96VZ6 Hybrid acetohydroxyacid synthase complex structure with cryptococcus neoformans ahas catalytic subunit and saccharomyces cerevisiae ahas regulatory subunit (see paper)
    40% identity, 96% coverage of query (400 bits)

6bd3A Saccharomyces cerevisiae acetohydroxyacid synthase
    40% identity, 92% coverage of query (397 bits)

6bd9A Saccharomyces cerevisiae acetohydroxyacid synthase
    41% identity, 92% coverage of query (395 bits)

1jscA Crystal structure of the catalytic subunit of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors
    41% identity, 92% coverage of query (395 bits)

IlvB / b3671 acetohydroxy acid synthase I subunit IlvB (EC 2.2.1.6) from Escherichia coli K-12 substr. MG1655 (see 7 papers)
ilvB / P08142 acetohydroxy acid synthase I subunit IlvB (EC 2.2.1.6) from Escherichia coli (strain K12) (see 4 papers)
P08142 acetolactate synthase (subunit 2/2) (EC 2.2.1.6) from Escherichia coli (see paper)
    39% identity, 96% coverage of query (386 bits)

6lpiB / P08142 Crystal structure of ahas holo-enzyme (see paper)
    40% identity, 96% coverage of query (383 bits)

PS417_08810 glyoxylate carboligase (EC 4.1.1.47) from Pseudomonas simiae WCS417
    35% identity, 89% coverage of query (297 bits)

Psest_1181 Glyoxylate carboligase (EC 4.1.1.47) from Pseudomonas stutzeri RCH2
    33% identity, 89% coverage of query (280 bits)

Gcl / b0507 glyoxylate carboligase (EC 4.1.1.47) from Escherichia coli K-12 substr. MG1655 (see 7 papers)
gcl / P0AEP7 glyoxylate carboligase (EC 4.1.1.47) from Escherichia coli (strain K12) (see 6 papers)
P0AEP7 tartronate-semialdehyde synthase (EC 4.1.1.47) from Escherichia coli K-12 (see paper)
gcl / ECOCYC|GLYOCARBOLIG-MONOMER glyoxylate carboligase; EC 4.1.1.47 from Escherichia coli K12 (see 5 papers)
gcl / AAA23864.1 glyoxylate carboligase from Escherichia coli (see paper)
    33% identity, 89% coverage of query (277 bits)

8i01A / P0AEP7 Crystal structure of escherichia coli glyoxylate carboligase (see paper)
    33% identity, 89% coverage of query (277 bits)

8beoB Crystal structure of e. Coli glyoxylate carboligase mutant i393a with map
    33% identity, 89% coverage of query (276 bits)

8beoA Crystal structure of e. Coli glyoxylate carboligase mutant i393a with map
    33% identity, 89% coverage of query (276 bits)

8i07D Crystal structure of escherichia coli glyoxylate carboligase double mutant in complex with glycolaldehyde
    33% identity, 89% coverage of query (276 bits)

8i07A Crystal structure of escherichia coli glyoxylate carboligase double mutant in complex with glycolaldehyde
    33% identity, 89% coverage of query (276 bits)

6u9dR / P07342 Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
    34% identity, 85% coverage of query (251 bits)

5ahkA / Q4K6F7 Crystal structure of acetohydroxy acid synthase pf5 from pseudomonas protegens
    31% identity, 84% coverage of query (219 bits)

Build an alignment

Build an alignment for WP_052306284.1 and 86 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

5dx6B / P27696 Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
    29% identity, 90% coverage of query (197 bits)

4rjiC Acetolactate synthase from bacillus subtilis bound to thdp - crystal form i (see paper)
    27% identity, 93% coverage of query (197 bits)

4rjkF Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii
    27% identity, 93% coverage of query (197 bits)

4rjkG Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii
    27% identity, 93% coverage of query (197 bits)

1ozgA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate
    29% identity, 90% coverage of query (194 bits)

5d6rB Acetolactate synthase from klebsiella pneumoniae in complex with mechanism-based inhibitor
    29% identity, 90% coverage of query (193 bits)

1ozfA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors
    29% identity, 90% coverage of query (192 bits)

3ey9A / P07003 Structural basis for membrane binding and catalytic activation of the peripheral membrane enzyme pyruvate oxidase from escherichia coli (see paper)
    28% identity, 93% coverage of query (192 bits)

PoxB / b0871 pyruvate oxidase (EC 1.2.5.1) from Escherichia coli K-12 substr. MG1655 (see 70 papers)
poxB / P07003 pyruvate oxidase (EC 1.2.5.1) from Escherichia coli (strain K12) (see 69 papers)
POXB_ECOLI / P07003 Pyruvate dehydrogenase [ubiquinone]; Pyruvate oxidase; POX; Pyruvate:ubiquinone-8 oxidoreductase; EC 1.2.5.1 from Escherichia coli (strain K12) (see 13 papers)
P07003 pyruvate dehydrogenase (quinone) (EC 1.2.5.1) from Escherichia coli (see paper)
    28% identity, 93% coverage of query (191 bits)

2djiA / A9X9K8 Crystal structure of pyruvate oxidase from aerococcus viridans containing fad (see paper)
    28% identity, 91% coverage of query (191 bits)

1v5gA Crystal structure of the reaction intermediate between pyruvate oxidase containing fad and tpp, and substrate pyruvate
    28% identity, 91% coverage of query (191 bits)

1v5fA Crystal structure of pyruvate oxidase complexed with fad and tpp, from aerococcus viridans
    28% identity, 91% coverage of query (191 bits)

8rphD 8rphD (see paper)
    28% identity, 87% coverage of query (187 bits)

8rpjA Janthe from janthinobacterium sp. Hh01
    28% identity, 87% coverage of query (187 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory