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Searching for up to 100 curated homologs for WP_066600301.1 NCBI__GCF_001046645.1:WP_066600301.1 (320 a.a.)

Found high-coverage hits (≥70%) to 100 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

U80676 / P93052 acyl-CoA reductase (NADH) from Botryococcus braunii (see 2 papers)
    82% identity, 100% coverage of query (527 bits)

mdh / Q84FY8 malate dehydrogenase subunit (EC 1.1.1.37) from Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (see paper)
Q84FY8 malate dehydrogenase (EC 1.1.1.37) from Methylorubrum extorquens (see paper)
    73% identity, 100% coverage of query (469 bits)

3gvhA / Q2YLR9 Crystal structure of lactate/malate dehydrogenase from brucella melitensis
    71% identity, 99% coverage of query (467 bits)

4rosA / A9W386 Crystal structure of methylobacterium extorquens malate dehydrogenase complexed with oxaloacetate and adenosine-5-diphosphoribose
    73% identity, 99% coverage of query (465 bits)

5ujkA Malate dehydrogenase from methylobacterium extorquens, complexed with NAD
    73% identity, 98% coverage of query (464 bits)

3gviA Crystal structure of lactate/malate dehydrogenase from brucella melitensis in complex with adp
    70% identity, 99% coverage of query (450 bits)

4pltB / A0A075B5H0 Crystal structure of ancestral apicomplexan malate dehydrogenase with oxamate. (see paper)
    65% identity, 97% coverage of query (407 bits)

4plyA Crystal structure of ancestral apicomplexan malate dehydrogenase with malate.
    65% identity, 97% coverage of query (407 bits)

4plfB / A0A075B5G8 Crystal structure of ancestral apicomplexan lactate dehydrogenase with lactate. (see paper)
    58% identity, 98% coverage of query (375 bits)

4plcA Crystal structure of ancestral apicomplexan lactate dehydrogenase with malate.
    58% identity, 98% coverage of query (375 bits)

6vdjA Crystal structure of ancestral apicomplexan lactate dehydrogenase with sulfate and nadh4.
    61% identity, 98% coverage of query (364 bits)

Q8I8U5 L-lactate dehydrogenase (EC 1.1.1.27) from Eimeria acervulina (see paper)
    52% identity, 99% coverage of query (343 bits)

P49814 Malate dehydrogenase; Vegetative protein 69; VEG69; EC 1.1.1.37 from Bacillus subtilis (strain 168)
    52% identity, 97% coverage of query (338 bits)

7byaA Malate dehydrogenase from geobacillus stearothermophilus (gs-mdh) complexed with oxaloacetic acid (oaa) and adenosine 5'- diphosphoribose (apr)
    52% identity, 97% coverage of query (337 bits)

7by9A Malate dehydrogenase from geobacillus stearothermophilus (gs-mdh) complexed with oxaloacetic acid (oaa) and nicotinamide adenine dinucleotide (NAD)
    52% identity, 97% coverage of query (337 bits)

7f8dA / A0A143T1U9 Malate dehydrogenase from geobacillus stearothermophilus (gs-mdh) g218y mutant (see paper)
    51% identity, 98% coverage of query (333 bits)

MDH_THET7 / A0A0S3QTC6 Malate dehydrogenase; MDH; EC 1.1.1.37 from Thermosulfidibacter takaii (strain DSM 17441 / JCM 13301 / NBRC 103674 / ABI70S6) (see paper)
    52% identity, 96% coverage of query (333 bits)

1uxgA / P80040 Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface. (see paper)
    54% identity, 98% coverage of query (326 bits)

Q27797 L-lactate dehydrogenase (EC 1.1.1.27) from Toxoplasma gondii (see paper)
    50% identity, 96% coverage of query (325 bits)

3czmB / Q27797 T. Gondii bradyzoite-specific ldh (ldh2) in complex with NAD and oxq
    50% identity, 96% coverage of query (325 bits)

MDH_CHLAA / P80040 Malate dehydrogenase; EC 1.1.1.37 from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) (see 3 papers)
    54% identity, 98% coverage of query (325 bits)

2hjrB / Q5CYZ3 Crystal structure of cryptosporidium parvum malate dehydrogenase (see paper)
    52% identity, 98% coverage of query (325 bits)

1sowA T. Gondii bradyzoite-specific ldh (ldh2) in complex with NAD and oxalate
    50% identity, 96% coverage of query (325 bits)

MDH_CHLTE / P80039 Malate dehydrogenase; EC 1.1.1.37 from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum) (see paper)
    53% identity, 98% coverage of query (323 bits)

1guzA / P0C890,P80039 Structural basis for thermophilic protein stability: structures of thermophilic and mesophilic malate dehydrogenases (see paper)
    52% identity, 97% coverage of query (318 bits)

6ct6B / U6M598 Crystal structure of lactate dehydrogenase from eimeria maxima with nadh and oxamate
    51% identity, 98% coverage of query (315 bits)

Q8I8U3 L-lactate dehydrogenase (EC 1.1.1.27) from Eimeria maxima (see paper)
    50% identity, 98% coverage of query (314 bits)

MDH_PROVB / P0C890 Malate dehydrogenase; EC 1.1.1.37 from Prosthecochloris vibrioformis (Chlorobium vibrioforme) (see paper)
    51% identity, 98% coverage of query (314 bits)

1gv0A / P80039 Structural basis for thermophilic protein stability: structures of thermophilic and mesophilic malate dehydrogenases (see paper)
    52% identity, 97% coverage of query (307 bits)

Q7Z0X7 L-lactate dehydrogenase (EC 1.1.1.27) from Babesia bovis (see paper)
CH_024078 L-lactate dehydrogenase; EC 1.1.1.27 from Babesia bovis T2Bo (see paper)
    48% identity, 96% coverage of query (305 bits)

Q9GT92 L-lactate dehydrogenase (EC 1.1.1.27) from Cryptosporidium parvum (see 2 papers)
    51% identity, 96% coverage of query (300 bits)

2fn7A / Q5CYZ2 Crystal structure of the lactate dehydrogenase from cryptosporidium parvum complexed with substrate (lactic acid) and cofactor (b- nicotinamide adenine dinucleotide)
    51% identity, 96% coverage of query (299 bits)

4nd2A Crystal structure of the lactate dehydrogenase from cryptosporidium parvum complexed with substrate (pyruvic acid) and cofactor analog (3-acetylpyridine adenine dinucleotide)
    51% identity, 96% coverage of query (299 bits)

Q8I8U4 L-lactate dehydrogenase (EC 1.1.1.27) from Eimeria tenella (see paper)
    50% identity, 96% coverage of query (285 bits)

2a92C / Q4PRK9 Crystal structure of lactate dehydrogenase from plasmodium vivax: complex with nadh (see paper)
    45% identity, 96% coverage of query (277 bits)

LDH lactate dehydrogenase; EC 1.1.1.27 from Plasmodium falciparum (see paper)
LDH / GB|ABA46354.1 lactate dehydrogenase; EC 1.1.1.27 from Plasmodium falciparum (see paper)
    44% identity, 96% coverage of query (274 bits)

2a94A Structure of plasmodium falciparum lactate dehydrogenase complexed to apadh.
    44% identity, 96% coverage of query (274 bits)

1t2dA Plasmodium falciparum lactate dehydrogenase complexed with NAD+ and oxalate
    44% identity, 96% coverage of query (274 bits)

1t25A Plasmodium falciparum lactate dehydrogenase complexed with nadh and 3- hydroxyisoxazole-4-carboxylic acid
    44% identity, 96% coverage of query (274 bits)

1t24A Plasmodium falciparum lactate dehydrogenase complexed with NAD+ and 4- hydroxy-1,2,5-oxadiazole-3-carboxylic acid
    44% identity, 96% coverage of query (274 bits)

3zh2A Structure of plasmodium falciparum lactate dehydrogenase in complex with a DNA aptamer
    44% identity, 96% coverage of query (274 bits)

1t26A Plasmodium falciparum lactate dehydrogenase complexed with nadh and 4- hydroxy-1,2,5-thiadiazole-3-carboxylic acid
    44% identity, 96% coverage of query (274 bits)

4b7uA Plasmodium falciparum l-lactate dehydrogenase complexed with bicine
    44% identity, 96% coverage of query (274 bits)

6txrB Structural insights into cubane-modified aptamer recognition of a malaria biomarker
    45% identity, 96% coverage of query (272 bits)

6txrA Structural insights into cubane-modified aptamer recognition of a malaria biomarker
    45% identity, 96% coverage of query (272 bits)

LDH_PLABA / Q7SI97 L-lactate dehydrogenase; EC 1.1.1.27 from Plasmodium berghei (strain Anka) (see paper)
    44% identity, 93% coverage of query (269 bits)

1u4oA Plasmodium falciparum lactate dehydrogenase complexed with 2,6- naphthalenedicarboxylic acid
    44% identity, 96% coverage of query (269 bits)

1u4sA Plasmodium falciparum lactate dehydrogenase complexed with 2,6- naphthalenedisulphonic acid
    44% identity, 96% coverage of query (269 bits)

1oc4A Lactate dehydrogenase from plasmodium berghei
    44% identity, 93% coverage of query (269 bits)

1u5aA Plasmodium falciparum lactate dehydrogenase complexed with 3,5- dihydroxy-2-naphthoic acid
    44% identity, 96% coverage of query (268 bits)

4b7uB Plasmodium falciparum l-lactate dehydrogenase complexed with bicine
    44% identity, 96% coverage of query (268 bits)

1u5cA Plasmodium falciparum lactate dehydrogenase complexed with 3,7- dihydroxynaphthalene-2-carboxylic acid and NAD+
    44% identity, 96% coverage of query (268 bits)

1xivA Plasmodium falciparum lactate dehydrogenase complexed with 2-({4- chloro-[hydroxy(methoxy)methyl]cyclohexyl}amino)ethane-1,1,2-triol
    44% identity, 96% coverage of query (268 bits)

Q6VVP7 malate dehydrogenase (EC 1.1.1.37) from Plasmodium falciparum (see paper)
GI|46361136 malate dehydrogenase; EC 1.1.1.37 from Plasmodium falciparum 3D7 (see paper)
    43% identity, 94% coverage of query (265 bits)

6r8gC / Q6VVP7 Crystal structure of malate dehydrogenase from plasmodium falciparum in complex with 4-(3,4-difluorophenyl)thiazol-2-amine
    43% identity, 94% coverage of query (265 bits)

MDH_FLAFR / Q25QU7 Malate dehydrogenase; EC 1.1.1.37 from Flavobacterium frigidimaris (see 2 papers)
    39% identity, 97% coverage of query (264 bits)

1cetA Chloroquine binds in the cofactor binding site of plasmodium falciparum lactate dehydrogenase.
    43% identity, 96% coverage of query (263 bits)

1t2eA / Q27743 Plasmodium falciparum lactate dehydrogenase s245a, a327p mutant complexed with nadh and oxamate (see paper)
    44% identity, 96% coverage of query (262 bits)

P90613 L-lactate dehydrogenase (EC 1.1.1.27) from Toxoplasma gondii (see 2 papers)
    47% identity, 97% coverage of query (259 bits)

3om9A / P90613 T. Gondii bradyzoite-specific ldh (ldh1) in complex with NAD and oxq
    47% identity, 97% coverage of query (259 bits)

1pzgA T.Gondii ldh1 complexed with apad and sulfate at 1.6 angstroms
    47% identity, 97% coverage of query (259 bits)

1pzfA T.Gondii ldh1 ternary complex with apad+ and oxalate
    47% identity, 97% coverage of query (259 bits)

MDH_AERPE / Q9YEA1 Malate dehydrogenase; EC 1.1.1.299 from Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) (see paper)
    39% identity, 96% coverage of query (237 bits)

MDH_PYRIL / A1RS58 Malate dehydrogenase; EC 1.1.1.37 from Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3) (see paper)
    38% identity, 95% coverage of query (226 bits)

A3MWU9 malate dehydrogenase (EC 1.1.1.37) from Pyrobaculum calidifontis (see paper)
    38% identity, 95% coverage of query (224 bits)

3pqdB / P13714 Crystal structure of l-lactate dehydrogenase from bacillus subtilis complexed with fbp and NAD+
    33% identity, 96% coverage of query (183 bits)

LDH_BACSU / P13714 L-lactate dehydrogenase; L-LDH; EC 1.1.1.27 from Bacillus subtilis (strain 168) (see paper)
    33% identity, 96% coverage of query (183 bits)

ldh / P00343 L-lactate dehydrogenase subunit (EC 1.1.1.27) from Lactobacillus casei (see 2 papers)
LDH_LACCA / P00343 L-lactate dehydrogenase; L-LDH; EC 1.1.1.27 from Lacticaseibacillus casei (Lactobacillus casei) (see 3 papers)
    31% identity, 96% coverage of query (182 bits)

1llcA / P00343 Structure determination of the allosteric l-lactate dehydrogenase from lactobacillus casei at 3.0 angstroms resolution
    31% identity, 96% coverage of query (182 bits)

LDH1_STAAE / A6QDL6 L-lactate dehydrogenase 1; L-LDH 1; EC 1.1.1.27 from Staphylococcus aureus (strain Newman) (see paper)
LDH1_STAAC / Q5HJD7 L-lactate dehydrogenase 1; L-LDH 1; EC 1.1.1.27 from Staphylococcus aureus (strain COL) (see paper)
    31% identity, 96% coverage of query (182 bits)

A0A2U9AUU1 L-lactate dehydrogenase (EC 1.1.1.27) from Lacticaseibacillus casei (see paper)
    31% identity, 96% coverage of query (182 bits)

6j9tB Complex structure of lactobacillus casei lactate dehydrogenase with fructose-1,6-bisphosphate
    31% identity, 96% coverage of query (182 bits)

3h3jB / Q5HJD7 Crystal structure of lactate dehydrogenase mutant (a85r) from staphylococcus aureus complexed with NAD and pyruvate
    31% identity, 96% coverage of query (180 bits)

Q5FMB0 L-lactate dehydrogenase (EC 1.1.1.27) from Lactobacillus acidophilus (see paper)
    31% identity, 97% coverage of query (179 bits)

4ln1B / Q81EP4 Crystal structure of l-lactate dehydrogenase from bacillus cereus atcc 14579 complexed with calcium, nysgrc target 029452
    31% identity, 96% coverage of query (177 bits)

LDH_GEOSE / P00344 L-lactate dehydrogenase; L-LDH; EC 1.1.1.27 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 3 papers)
    31% identity, 95% coverage of query (176 bits)

1ldnA / P00344 Structure of a ternary complex of an allosteric lactate dehydrogenase from bacillus stearothermophilus at 2.5 angstroms resolution (see paper)
    31% identity, 95% coverage of query (176 bits)

W8CXQ9 L-lactate dehydrogenase (EC 1.1.1.27) from Weizmannia coagulans (see paper)
    30% identity, 96% coverage of query (172 bits)

Build an alignment

Build an alignment for WP_066600301.1 and 78 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

LDH_LACHE / O32765 L-lactate dehydrogenase; L-LDH; EC 1.1.1.27 from Lactobacillus helveticus (Lactobacillus suntoryeus) (see paper)
    29% identity, 98% coverage of query (172 bits)

ldh1 L-lactate dehydrogenase 1; EC 1.1.1.27 from Lactobacillus plantarum (see paper)
    32% identity, 96% coverage of query (171 bits)

B9MKZ8 L-lactate dehydrogenase (EC 1.1.1.27) from Caldicellulosiruptor bescii (see paper)
    32% identity, 97% coverage of query (171 bits)

1ez4B / P56511 Crystal structure of non-allosteric l-lactate dehydrogenase from lactobacillus pentosus at 2.3 angstrom resolution (see paper)
    32% identity, 96% coverage of query (170 bits)

LDH_LACPE / P56511 L-lactate dehydrogenase; L-LDH; EC 1.1.1.27 from Lactiplantibacillus pentosus (Lactobacillus pentosus) (see 2 papers)
ldh L-lactate dehydrogenase; EC 1.1.1.27 from Lactobacillus pentosus (see paper)
    32% identity, 96% coverage of query (170 bits)

LDH_LACLN / D8KFT1 L-lactate dehydrogenase; L-LDH; EC 1.1.1.27 from Lactococcus lactis subsp. cremoris (strain NZ9000) (see paper)
    30% identity, 98% coverage of query (170 bits)

P00345 L-lactate dehydrogenase (EC 1.1.1.27) from Priestia megaterium (see paper)
    31% identity, 95% coverage of query (170 bits)

A0A1A6GB48 L-lactate dehydrogenase (EC 1.1.1.27) from Enterococcus mundtii (see paper)
    30% identity, 98% coverage of query (167 bits)

B1A4R6 L-lactate dehydrogenase (EC 1.1.1.27) from Thermoanaerobacter ethanolicus (see paper)
    31% identity, 96% coverage of query (166 bits)

6j9uA Complex structure of lactobacillus casei lactate dehydrogenase penta mutant with pyruvate
    29% identity, 96% coverage of query (166 bits)

1a5zA / P16115 Lactate dehydrogenase from thermotoga maritima (tmldh) (see paper)
    35% identity, 88% coverage of query (164 bits)

LDH_THEMA / P16115 L-lactate dehydrogenase; L-LDH; EC 1.1.1.27 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see 2 papers)
    35% identity, 88% coverage of query (164 bits)

3pqdD Crystal structure of l-lactate dehydrogenase from bacillus subtilis complexed with fbp and NAD+
    32% identity, 96% coverage of query (164 bits)

MDH_ARCFU / O08349 Malate dehydrogenase; EC 1.1.1.37 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see 2 papers)
O08349 malate dehydrogenase (EC 1.1.1.37); L-2-hydroxycarboxylate dehydrogenase [NAD(P)+] (EC 1.1.1.375) from Archaeoglobus fulgidus (see 2 papers)
2x0iA / O08349 2.9 a resolution structure of malate dehydrogenase from archaeoglobus fulgidus in complex with nadh (see paper)
    32% identity, 96% coverage of query (162 bits)

ldh / AAA99896.1 L-lactate:NAD+ oxidoreductase from Lactococcus lactis (see 2 papers)
ldh / AAA25172.1 lactate dehydrogenase from Lactococcus lactis (see paper)
    32% identity, 96% coverage of query (162 bits)

Q8CMZ0 L-lactate dehydrogenase (EC 1.1.1.27) from Staphylococcus epidermidis (see paper)
    28% identity, 97% coverage of query (162 bits)

2x0jA 2.8 a resolution structure of malate dehydrogenase from archaeoglobus fulgidus in complex with etheno-NAD
    32% identity, 96% coverage of query (162 bits)

8rwlA / Q8TWG5 Crystal structure of methanopyrus kandleri malate dehydrogenase mutant 1
    34% identity, 95% coverage of query (161 bits)

ldhA L-lactate dehydrogenase 1; EC 1.1.1.27 from Lactococcus lactis subsp. lactis (see paper)
    32% identity, 96% coverage of query (161 bits)

LDH6A_HUMAN / Q6ZMR3 L-lactate dehydrogenase A-like 6A; LDHA-like protein 6A; EC 1.1.1.27 from Homo sapiens (Human) (see paper)
    30% identity, 98% coverage of query (161 bits)

P59390 L-lactate dehydrogenase (EC 1.1.1.27) from Lactiplantibacillus plantarum (see paper)
    31% identity, 93% coverage of query (160 bits)

LDH_PEDAC / Q59645 L-lactate dehydrogenase; L-LDH; EC 1.1.1.27 from Pediococcus acidilactici (see paper)
    30% identity, 96% coverage of query (160 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory