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Searching for up to 100 curated homologs for WP_077278977.1 NCBI__GCF_002000365.1:WP_077278977.1 (516 a.a.)

Found high-coverage hits (≥70%) to 57 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

LEU1_NEIMB / Q9JZG1 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Neisseria meningitidis serogroup B (strain MC58) (see 2 papers)
Q9JZG1 2-isopropylmalate synthase (EC 2.3.3.13) from Neisseria meningitidis (see 2 papers)
    66% identity, 97% coverage of query (699 bits)

LEU11_LEPIN / Q8F445 2-isopropylmalate synthase 1; Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1; EC 2.3.3.13 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see paper)
    55% identity, 97% coverage of query (548 bits)

LeuA / b0074 2-isopropylmalate synthase (EC 2.3.3.13) from Escherichia coli K-12 substr. MG1655 (see 7 papers)
leuA / P09151 2-isopropylmalate synthase (EC 2.3.3.13) from Escherichia coli (strain K12) (see 15 papers)
    51% identity, 97% coverage of query (495 bits)

LEU1_SALTY / P15875 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 3 papers)
    50% identity, 97% coverage of query (489 bits)

Q72JC9 2-isopropylmalate synthase (EC 2.3.3.13) from Thermus thermophilus (see 2 papers)
    48% identity, 99% coverage of query (474 bits)

K4C627 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum lycopersicum (see paper)
    49% identity, 96% coverage of query (469 bits)

BT1861 2-isopropylmalate synthase (EC 2.3.3.13) from Bacteroides thetaiotaomicron VPI-5482
    49% identity, 97% coverage of query (467 bits)

Q30DX9 2-isopropylmalate synthase (EC 2.3.3.13) from Brassica insularis (see paper)
    47% identity, 98% coverage of query (461 bits)

Q2RAP8 2-isopropylmalate synthase (EC 2.3.3.13) from Oryza sativa Japonica Group (see paper)
    48% identity, 96% coverage of query (457 bits)

LEU11_ARATH / Q9LPR4 2-isopropylmalate synthase 1, chloroplastic; Methylthioalkylmalate synthase-like 4; EC 2.3.3.13 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
Q9LPR4 2-isopropylmalate synthase (EC 2.3.3.13) from Arabidopsis thaliana (see 3 papers)
    47% identity, 98% coverage of query (457 bits)

Q2QXY9 2-isopropylmalate synthase (EC 2.3.3.13) from Oryza sativa Japonica Group (see paper)
    48% identity, 96% coverage of query (455 bits)

LEU12_ARATH / Q9C550 2-isopropylmalate synthase 2, chloroplastic; 2-isopropylmalate synthase 1; Methylthioalkylmalate synthase-like 3; EC 2.3.3.13 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9C550 2-isopropylmalate synthase (EC 2.3.3.13) from Arabidopsis thaliana (see 2 papers)
    47% identity, 97% coverage of query (455 bits)

D0VY45 homocitrate synthase (EC 2.3.3.14) from Lotus japonicus (see paper)
    47% identity, 96% coverage of query (429 bits)

LEU1_METJA / Q58595 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58595 2-isopropylmalate synthase (EC 2.3.3.13) from Methanocaldococcus jannaschii (see paper)
    47% identity, 97% coverage of query (426 bits)

CA265_RS15855 2-isopropylmalate synthase (EC 2.3.3.13) from Pedobacter sp. GW460-11-11-14-LB5
    52% identity, 73% coverage of query (404 bits)

LEU1_SOYBN / Q39891 Probable 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; Late nodulin-56; N-56; EC 2.3.3.13 from Glycine max (Soybean) (Glycine hispida) (see paper)
    44% identity, 97% coverage of query (401 bits)

Echvi_3833 2-isopropylmalate synthase (EC 2.3.3.13) from Echinicola vietnamensis KMM 6221, DSM 17526
    51% identity, 73% coverage of query (395 bits)

6e1jA / C5J4P1 Crystal structure of methylthioalkylmalate synthase (bjumam1.1) from brassica juncea (see paper)
    49% identity, 73% coverage of query (386 bits)

MAM3 / Q9FN52 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see paper)
MAM3_ARATH / Q9FN52 Methylthioalkylmalate synthase 3, chloroplastic; 2-isopropylmalate synthase 2; Methylthioalkylmalate synthase-like; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9FN52 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see 4 papers)
    49% identity, 73% coverage of query (381 bits)

cimA / Q58787 (R)-citratemalate synthase subunit (EC 2.3.3.21) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see paper)
CIMA_METJA / Q58787 (R)-citramalate synthase CimA; EC 2.3.3.21 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58787 (R)-citramalate synthase (EC 2.3.3.21) from Methanocaldococcus jannaschii (see 2 papers)
    43% identity, 96% coverage of query (374 bits)

MAM1 / Q9FG67 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see 2 papers)
MAM1_ARATH / Q9FG67 Methylthioalkylmalate synthase 1, chloroplastic; 2-isopropylmalate synthase 3; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
Q9FG67 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see 7 papers)
    48% identity, 73% coverage of query (373 bits)

K4CJ56 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum lycopersicum (see paper)
    49% identity, 74% coverage of query (370 bits)

MAM2_ARATH / Q8VX04 Methylthioalkylmalate synthase 2, chloroplastic; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q8VX04 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see paper)
    48% identity, 73% coverage of query (370 bits)

A0A0B5GPY2 methylthioalkylmalate synthase (EC 2.3.3.17) from Brassica oleracea (see paper)
    44% identity, 72% coverage of query (335 bits)

LEU1_SULAC / Q4JA78 2-isopropylmalate synthase; IPMS; Alpha-isopropylmalate synthase; Alpha-IPM synthase; EC 2.3.3.13 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
    46% identity, 73% coverage of query (316 bits)

AKSA_METJA / Q57926 Homocitrate synthase AksA; (R)-homo(2)citrate synthase; (R)-homo(3)citrate synthase; EC 2.3.3.14; EC 2.3.3.- from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
    41% identity, 73% coverage of query (265 bits)

P58637 homocitrate synthase (EC 2.3.3.14) from Nostoc sp. PCC 7120 = FACHB-418 (see paper)
    36% identity, 73% coverage of query (209 bits)

BT1858 (R)-citramalate synthase (EC 2.3.1.182) from Bacteroides thetaiotaomicron VPI-5482
    30% identity, 96% coverage of query (197 bits)

B0SN40 2-isopropylmalate synthase (EC 2.3.3.13) from Leptospira biflexa (see paper)
    32% identity, 73% coverage of query (194 bits)

cimA / Q74C76 (R)-citramalate synthase (EC 2.3.3.21) from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) (see paper)
CIMA_GEOSL / Q74C76 (R)-citramalate synthase; Citramalate synthase; EC 2.3.3.21 from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) (see paper)
Q74C76 (R)-citramalate synthase (EC 2.3.3.21) from Geobacter sulfurreducens (see paper)
    30% identity, 95% coverage of query (188 bits)

4ov9A / B0SN40 Structure of isopropylmalate synthase binding with alpha- isopropylmalate (see paper)
    32% identity, 73% coverage of query (187 bits)

4ov4A Isopropylmalate synthase binding with ketoisovalerate
    32% identity, 73% coverage of query (187 bits)

A0A1Y6KBA1 homocitrate synthase (EC 2.3.3.14) from Bradyrhizobium sp. ORS 285 (see 2 papers)
    35% identity, 75% coverage of query (182 bits)

Build an alignment

Build an alignment for WP_077278977.1 and 33 homologs with ≥ 30% identity

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Change minimum %identity:

Additional hits (identity < 30%)

CIMA_SULAC / Q4J6H1 (R)-citramalate synthase; Citramalate synthase; CMS; EC 2.3.3.21 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
    27% identity, 95% coverage of query (161 bits)

cimA / Q8F3Q1 (R)-citramalate synthase subunit (EC 2.3.3.21) from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see paper)
CIMA_LEPIN / Q8F3Q1 (R)-citramalate synthase CimA; LiCMS; EC 2.3.3.21 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see 3 papers)
    27% identity, 97% coverage of query (159 bits)

B0K6M2 (R)-citramalate synthase (EC 2.3.3.21) from Thermoanaerobacter sp. (see paper)
    27% identity, 95% coverage of query (155 bits)

DVU1914 (R)-citramalate synthase (EC 2.3.3.21) from Desulfovibrio vulgaris Hildenborough JW710
    26% identity, 89% coverage of query (145 bits)

3ivtB / Q9Y823 Homocitrate synthase lys4 bound to 2-og (see paper)
    30% identity, 71% coverage of query (143 bits)

HOSM_SCHPO / Q9Y823 Homocitrate synthase, mitochondrial; HCS; EC 2.3.3.14 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
lys4 / RF|NP_596458.1 homocitrate synthase (predicted); EC 2.3.3.14 from Schizosaccharomyces pombe (see 2 papers)
    30% identity, 71% coverage of query (143 bits)

CIRSY_DEHMC / P0DO96 Citrate (Re)-synthase; Re face-specific citrate synthase; Re-citrate synthase; EC 2.3.3.3 from Dehalococcoides mccartyi (strain CBDB1) (see paper)
P0DO96 citrate (Re)-synthase (EC 2.3.3.3) from Dehalococcoides sp. (see paper)
    30% identity, 72% coverage of query (141 bits)

3mi3A Homocitrate synthase lys4 bound to lysine
    30% identity, 71% coverage of query (140 bits)

LYS20 / P48570 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 6 papers)
HOSC_YEAST / P48570 Homocitrate synthase, cytosolic isozyme; HCS; EC 2.3.3.14 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
P48570 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (see 2 papers)
    29% identity, 71% coverage of query (139 bits)

LYS21 / Q12122 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 6 papers)
HOSM_YEAST / Q12122 Homocitrate synthase, mitochondrial; HCS; EC 2.3.3.14 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
Q12122 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (see paper)
    28% identity, 77% coverage of query (139 bits)

LEU1_YEAST / P06208 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
    26% identity, 98% coverage of query (138 bits)

C7C437 homocitrate synthase (EC 2.3.3.14) from Aspergillus fumigatus (see paper)
    29% identity, 71% coverage of query (138 bits)

M1U949 homocitrate synthase (EC 2.3.3.14) from Flammulina velutipes (see paper)
    27% identity, 73% coverage of query (137 bits)

3ivsA Homocitrate synthase lys4
    30% identity, 73% coverage of query (137 bits)

LEU9_YEAST / Q12166 2-isopropylmalate synthase 2, mitochondrial; Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2; Alpha-isopropylmalate synthase II; EC 2.3.3.13 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
    26% identity, 96% coverage of query (136 bits)

CH_124190 2-isopropylmalate synthase 2 from Magnaporthe grisea 70-15 (see paper)
    25% identity, 94% coverage of query (128 bits)

LEU4 putative 2-isopropylmalalate synthase from Candida albicans (see paper)
    27% identity, 96% coverage of query (126 bits)

LEU1_CORGL / P42455 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
    27% identity, 96% coverage of query (124 bits)

leuA / CAA50295.1 2-isopropylmalate synthase from Corynebacterium glutamicum (see paper)
    27% identity, 96% coverage of query (124 bits)

3hpzB Crystal structure of mycobacterium tuberculosis leua complexed with bromopyruvate
    26% identity, 95% coverage of query (116 bits)

3figB / P9WQB3 Crystal structure of leucine-bound leua from mycobacterium tuberculosis (see paper)
    26% identity, 95% coverage of query (115 bits)

3hq1A Crystal structure of mycobacterium tuberculosis leua complexed with citrate and mn2+
    26% identity, 95% coverage of query (115 bits)

1sr9A Crystal structure of leua from mycobacterium tuberculosis
    26% identity, 95% coverage of query (115 bits)

3hpsA Crystal structure of mycobacterium tuberculosis leua complexed with ketoisocaproate (kic)
    26% identity, 95% coverage of query (114 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory