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Searching for up to 100 curated homologs for WP_077279608.1 NCBI__GCF_002000365.1:WP_077279608.1 (242 a.a.)

Found high-coverage hits (≥70%) to 48 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Psest_0155 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Pseudomonas stutzeri RCH2
    62% identity, 100% coverage of query (318 bits)

Pf1N1B4_1624 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Pseudomonas fluorescens FW300-N1B4
    61% identity, 100% coverage of query (317 bits)

AO353_12085 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Pseudomonas fluorescens FW300-N2E3
    61% identity, 100% coverage of query (317 bits)

AO356_09800 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Pseudomonas fluorescens FW300-N2C3
    61% identity, 100% coverage of query (317 bits)

Pf6N2E2_3839 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Pseudomonas fluorescens FW300-N2E2
    61% identity, 100% coverage of query (316 bits)

PP_0292 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Pseudomonas putida KT2440
    61% identity, 100% coverage of query (316 bits)

PfGW456L13_359 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Pseudomonas fluorescens GW456-L13
    61% identity, 100% coverage of query (316 bits)

PS417_01575 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Pseudomonas simiae WCS417
    61% identity, 100% coverage of query (314 bits)

HSERO_RS20320 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Herbaspirillum seropedicae SmR1
    59% identity, 100% coverage of query (305 bits)

BPHYT_RS17685 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Burkholderia phytofirmans PsJN
    61% identity, 100% coverage of query (304 bits)

hisA / BAC65275.1 phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase from Burkholderia multivorans (see paper)
    61% identity, 100% coverage of query (293 bits)

SMc02570 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Sinorhizobium meliloti 1021
    51% identity, 99% coverage of query (234 bits)

AZOBR_RS01680 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Azospirillum brasilense Sp245
    51% identity, 99% coverage of query (232 bits)

CCNA_03852 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Caulobacter crescentus NA1000
    50% identity, 98% coverage of query (219 bits)

Ga0059261_1047 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Sphingomonas koreensis DSMZ 15582
    47% identity, 96% coverage of query (202 bits)

Echvi_2515 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Echinicola vietnamensis KMM 6221, DSM 17526
    39% identity, 100% coverage of query (162 bits)

BT1379 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Bacteroides thetaiotaomicron VPI-5482
    40% identity, 93% coverage of query (152 bits)

HIS4_STRCO / P16250 Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16; EC 5.3.1.24 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
P16250 phosphoribosylanthranilate isomerase (EC 5.3.1.24) from Streptomyces coelicolor (see paper)
5dn1A / P16250 Crystal structure of phosphoribosyl isomerase a from streptomyces coelicolor (see paper)
    38% identity, 97% coverage of query (151 bits)

4tx9A / B5I4P8 Crystal structure of hisap from streptomyces sviceus with degraded profar (see paper)
    38% identity, 97% coverage of query (141 bits)

HIS4_THEMA / Q9X0C7 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
    31% identity, 99% coverage of query (132 bits)

3zs4A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound prfar
    35% identity, 98% coverage of query (132 bits)

CA265_RS03615 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Pedobacter sp. GW460-11-11-14-LB5
    34% identity, 98% coverage of query (131 bits)

2y88A / P9WMM5 Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase (variant d11n) with bound prfar (see paper)
    35% identity, 98% coverage of query (131 bits)

2y85A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound rcdrp
    34% identity, 98% coverage of query (124 bits)

P10372 1-(5-phosphoribosyl)-5-[(5 phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Salmonella enterica subsp. enterica serovar Typhimurium (see paper)
    36% identity, 97% coverage of query (122 bits)

hisA / CAQ00038.1 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase from Rhodococcus erythropolis (see paper)
    32% identity, 98% coverage of query (122 bits)

HisA / b2024 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
hisA / P10371 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Escherichia coli (strain K12) (see 2 papers)
    35% identity, 97% coverage of query (121 bits)

5abtA / P10372 S.Enterica hisa mutant d7n, g102a, v106m, d176a
    34% identity, 97% coverage of query (115 bits)

5ab3A S.Enterica hisa mutant d7n, d10g, dup13-15, q24l, g102a
    33% identity, 97% coverage of query (102 bits)

A4WHB6 imidazole glycerol-phosphate synthase (subunit 2/2) (EC 4.3.2.10) from Pyrobaculum arsenaticum (see paper)
    32% identity, 83% coverage of query (90.1 bits)

hisF / BAC65276.1 imidazole glycerol phosphate synthase from Burkholderia multivorans (see paper)
    32% identity, 98% coverage of query (87.0 bits)

Build an alignment

Build an alignment for WP_077279608.1 and 31 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

Q5NMD6 imidazole glycerol-phosphate synthase (subunit 2/2) (EC 4.3.2.10) from Zymomonas mobilis (see paper)
    28% identity, 98% coverage of query (84.0 bits)

Q8ZY16 imidazole glycerol-phosphate synthase (subunit 2/2) (EC 4.3.2.10) from Pyrobaculum aerophilum (see paper)
    32% identity, 82% coverage of query (83.2 bits)

1h5yB / Q8ZY16 Hisf protein from pyrobaculum aerophilum (see paper)
    31% identity, 82% coverage of query (80.9 bits)

hisF / Q9X0C6 imidazole-glycerol-phosphate synthase cycloligase subunit (EC 4.3.2.10) from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
HIS6_THEMA / Q9X0C6 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
Q9X0C6 imidazole glycerol-phosphate synthase (subunit 2/2) (EC 4.3.2.10) from Thermotoga maritima (see 5 papers)
    29% identity, 83% coverage of query (80.9 bits)

7ac8A Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex.
    29% identity, 83% coverage of query (80.9 bits)

3zr4E Structural evidence for ammonia tunneling across the (beta-alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex
    29% identity, 83% coverage of query (80.9 bits)

1gpwC Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex.
    29% identity, 79% coverage of query (80.5 bits)

HisF / b2025 imidazole glycerol phosphate synthase subunit HisF (EC 4.3.2.10) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
hisF / P60664 imidazole glycerol phosphate synthase subunit HisF (EC 4.3.2.10) from Escherichia coli (strain K12) (see 7 papers)
HIS6_ECOLI / P60664 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Escherichia coli (strain K12) (see paper)
hisF / RF|NP_416529 IGP synthase cyclase subunit; EC 4.1.3.- from Escherichia coli K12 (see 7 papers)
    24% identity, 97% coverage of query (78.6 bits)

7qc8A / Q9X0C6 7qc8A (see paper)
    29% identity, 83% coverage of query (76.3 bits)

2wjzE Crystal structure of (hish) k181a y138a mutant of imidazoleglycerolphosphate synthase (hish hisf) which displays constitutive glutaminase activity
    28% identity, 83% coverage of query (72.8 bits)

HIS6_THET8 / Q7SIB9 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
Q7SIB9 imidazole glycerol-phosphate synthase (subunit 1/2) (EC 4.3.2.10) from Thermus thermophilus (see paper)
    29% identity, 84% coverage of query (70.5 bits)

4ewnD Structure of hisf-d130v+d176v with bound rcdrp
    28% identity, 83% coverage of query (67.4 bits)

5d2tA Directed evolutionary changes in kemp eliminase ke07 - crystal 3 wild type
    26% identity, 98% coverage of query (67.4 bits)

3iivB Evolutionary optimization of computationally designed enzymes: kemp eliminases of the ke07 series (see paper)
    29% identity, 80% coverage of query (61.2 bits)

6dnjA Directed evolutionary changes in kemp eliminase ke07 - crystal 28 round 5
    26% identity, 82% coverage of query (61.2 bits)

Q5TKS8 1-(5-phosphoribosyl)-5-[(5 phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (EC 5.3.1.16) from Arabidopsis thaliana (see paper)
    25% identity, 92% coverage of query (48.5 bits)

HISN3 / O82782 phosphoribosyl-formimino-5-aminoimidazole-4-carboxamide ribonucleotide isomerase (EC 5.3.1.16) from Arabidopsis thaliana (see paper)
HIS3_ARATH / O82782 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic; 5-proFAR isomerase; BBM II isomerase; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; Protein ALBINO AND PALE GREEN 10; Protein HISTIDINE BIOSYNTHESIS 3; EC 5.3.1.16 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    25% identity, 92% coverage of query (47.8 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory