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Searching for up to 100 curated homologs for WP_081943716.1 NCBI__GCF_000763315.1:WP_081943716.1 (418 a.a.)

Found high-coverage hits (≥70%) to 36 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

NUPC_BACSU / P39141 Nucleoside permease NupC from Bacillus subtilis (strain 168) (see paper)
TC 2.A.41.1.2 / P39141 Pyrimidine-specific nucleoside:H+ symporter, NupC from Bacillus subtilis (see 4 papers)
    59% identity, 92% coverage of query (451 bits)

Cru / b2393 nucleoside:H+ symporter NupC from Escherichia coli K-12 substr. MG1655 (see 6 papers)
nupC / P0AFF2 nucleoside:H+ symporter NupC from Escherichia coli (strain K12) (see 13 papers)
NUPC_ECOLI / P0AFF2 Nucleoside permease NupC; Nucleoside-transport system protein NupC from Escherichia coli (strain K12) (see 6 papers)
TC 2.A.41.1.1 / P0AFF2 Pyrimidine nucleoside:H+ symporter, NupC (Craig et al. 1994; Patching et al. 2005). Wild-type NupC had an apparent affinity for uridine of 22.2 +/- 3.7 muM and an apparent binding affinity of 1.8-2.6 mM, and various mutants with alterred properties were isolated and characterized (Sun et al. 2017). ADP-glucose is also a substrate of this system from Escherichia coli (see 5 papers)
    51% identity, 94% coverage of query (395 bits)

nupC / CAA52821.1 NupC from Escherichia coli (see paper)
    51% identity, 94% coverage of query (389 bits)

NUPG_BACSU / P42312 Purine nucleoside transport protein NupG from Bacillus subtilis (strain 168) (see paper)
TC 2.A.41.1.3 / P42312 The purine nucleoside uptake transporter NupG (YxjA) from Bacillus subtilis (see 4 papers)
    37% identity, 93% coverage of query (256 bits)

NupX / b2161 putative nucleoside transporter from Escherichia coli K-12 substr. MG1655 (see 2 papers)
nupX / P33021 putative nucleoside transporter from Escherichia coli (strain K12) (see paper)
NUPX_ECOLI / P33021 Putative nucleoside permease NupX from Escherichia coli (strain K12) (see paper)
TC 2.A.41.2.10 / P33021 Nucleoside permease NupX from Escherichia coli (strain K12) (see 4 papers)
    31% identity, 93% coverage of query (191 bits)

Shewana3_1039 nucleoside permease (cytidine, inosine, uridine, or thymidine) from Shewanella sp. ANA-3
    30% identity, 94% coverage of query (186 bits)

PsuT / b2164 putative pseudouridine transporter from Escherichia coli K-12 substr. MG1655 (see 4 papers)
    30% identity, 94% coverage of query (178 bits)

5l26A Structure of cntnw in an inward-facing substrate-bound state
    30% identity, 93% coverage of query (176 bits)

Build an alignment

Build an alignment for WP_081943716.1 and 8 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

nupC / O25792 purine nucleobase/nucleoside transporter from Helicobacter pylori (strain ATCC 700392 / 26695) (see 2 papers)
NUPC_HELPY / O25792 Nucleoside permease NupC from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
    29% identity, 94% coverage of query (175 bits)

5l2aA / G4CRQ5 Structure of cntnw n149s,f366a in an outward-facing state (see paper)
    29% identity, 93% coverage of query (173 bits)

TC 2.A.41.2.11 / Q9KPL5 Concentrative nucleoside transporter, CNT, of 418 aas and 12 TMSs. A repeat-swapped model of VcCNT predicts that nucleoside transport occurs via a mechanism involving an elevator-like substrate binding domain movement across the membrane from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
    30% identity, 93% coverage of query (168 bits)

4pd7A Structure of vccnt bound to zebularine
    30% identity, 93% coverage of query (168 bits)

3tijA Crystal structure of a concentrative nucleoside transporter from vibrio cholerae
    30% identity, 93% coverage of query (168 bits)

4pb1A / Q9KPL5 Structure of vccnt-7c8c bound to ribavirin (see paper)
    30% identity, 93% coverage of query (167 bits)

4pdaA Structure of vccnt-7c8c bound to cytidine
    30% identity, 93% coverage of query (167 bits)

4pd9A Structure of vccnt-7c8c bound to adenosine
    30% identity, 93% coverage of query (167 bits)

4pd8A Structure of vccnt-7c8c bound to pyrrolo-cytidine
    30% identity, 93% coverage of query (167 bits)

4pb2A Structure of vccnt-7c8c bound to 5-fluorouridine
    30% identity, 93% coverage of query (167 bits)

4pd5A Crystal structure of vccnt-7c8c bound to gemcitabine
    30% identity, 93% coverage of query (167 bits)

S28A3_MOUSE / Q9ERH8 Solute carrier family 28 member 3; Concentrative Na(+)-nucleoside cotransporter 3; CNT 3; mCNT3 from Mus musculus (Mouse) (see paper)
TC 2.A.41.2.6 / Q9ERH8 Broad-specificity nucleoside:Na+, H+ and Li+ symporter, hCNT3 (Slc28a3) transports a broad range of both purine and pyrimidine nucleosides as well as anticancer and antiviral nucleoside drugs, but guanosine, 3'azido-3-deoxythymidine and 2',3'-dideoxycytidine, which are substrates with Na+, are not substrates with H+. Both of the two cation-binding sites can apparently bind Na+, but only one can bind H+, and the Na+ and H+ forms transport different ranges of substrates. (Note: Cnt1 and Cnt2 are Na+-specific.) from Homo sapiens (Human) (see 3 papers)
Slc28a3 / RF|NP_071712.3 solute carrier family 28 member 3 from Mus musculus (see paper)
    26% identity, 92% coverage of query (149 bits)

S28A3_RAT / Q8VIH3 Solute carrier family 28 member 3; Concentrative Na(+)-nucleoside cotransporter 3; CNT 3; rCNT3 from Rattus norvegicus (Rat) (see paper)
    26% identity, 92% coverage of query (145 bits)

TC 2.A.41.2.5 / Q9UA35 Broadly selective nucleoside:Na+ cotransporter, hfCNT (transports uridine, thymidine, inosine, 3'-azido-3'deoxythymidine, 2'3'dideoxycytidine, and 2'3'dideoxyinosine) (Na+/uridine = 2) from Eptatretus stoutii (Pacific hagfish) (see paper)
    27% identity, 94% coverage of query (145 bits)

8tz1A / F1MGR1 Cryo-em structure of bovine concentrative nucleoside transporter 3 in complex with ribavirin (see paper)
    25% identity, 92% coverage of query (138 bits)

8tz7A Cryo-em structure of bovine concentrative nucleoside transporter 3 in complex with molnupiravir, condition 1, int1-int1-int1 conformation
    25% identity, 92% coverage of query (138 bits)

8tz6A Cryo-em structure of bovine concentrative nucleoside transporter 3 in complex with psi-6206
    25% identity, 92% coverage of query (138 bits)

8tz5A Cryo-em structure of bovine concentrative nucleoside transporter 3 in complex with n-hydroxycytidine
    25% identity, 92% coverage of query (138 bits)

8tz4A Cryo-em structure of bovine concentrative nucleoside transporter 3 in complex with gs-441524, subset reconstruction
    25% identity, 92% coverage of query (138 bits)

S28A3_HUMAN / Q9HAS3 Solute carrier family 28 member 3; Concentrative Na(+)-nucleoside cotransporter 3; CNT 3; hCNT3 from Homo sapiens (Human) (see 8 papers)
TC 2.A.41.2.8 / Q9HAS3 Solute carrier family 28 member 3 (Concentrative Na+-nucleoside cotransporter 3) (CNT 3) (hCNT3) from Homo sapiens (see 9 papers)
    25% identity, 92% coverage of query (137 bits)

cntA nucleoside transporter (Eurofung) from Emericella nidulans (see paper)
    25% identity, 92% coverage of query (129 bits)

S28A1_RAT / Q62674 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Rattus norvegicus (Rat) (see 3 papers)
TC 2.A.41.2.2 / Q62674 Nonspecific nucleoside:Na+ symporter from Rattus norvegicus (Rat) (see paper)
Slc28a1 / RF|NP_446315.1 sodium/nucleoside cotransporter 1 from Rattus norvegicus
    24% identity, 92% coverage of query (115 bits)

S28A1_PIG / O62667 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Sus scrofa (Pig) (see paper)
    24% identity, 92% coverage of query (113 bits)

S28A2_RAT / Q62773 Sodium/nucleoside cotransporter 2; Concentrative nucleoside transporter 2; CNT 2; rCNT2; Na(+)/nucleoside cotransporter 2; Sodium-coupled nucleoside transporter 2; Sodium/purine nucleoside cotransporter; SPNT; Solute carrier family 28 member 2 from Rattus norvegicus (Rat) (see 3 papers)
TC 2.A.41.2.1 / Q62773 Purine-specific nucleoside:Na+ symporter (see 3 papers)
    24% identity, 92% coverage of query (113 bits)

S28A1_RABIT / Q9MZT2 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Oryctolagus cuniculus (Rabbit) (see paper)
    23% identity, 92% coverage of query (112 bits)

S28A1_HUMAN / O00337 Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; hCNT1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1 from Homo sapiens (Human) (see 9 papers)
TC 2.A.41.2.3 / O00337 Pyrimidine-preferring nucleoside:Na+ symporter, CNT1 (Na+/nucleoside = 2)(transports uridine, gemcitabine and 5'-deoxy-5'-fluorouridine) (Larráyoz et al., 2004), but in addition to pyrimidine nucelosides, it transports adenosine from Homo sapiens (Human) (see 5 papers)
    24% identity, 92% coverage of query (112 bits)

S28A2_HUMAN / O43868 Sodium/nucleoside cotransporter 2; Concentrative nucleoside transporter 2; CNT 2; hCNT2; Na(+)/nucleoside cotransporter 2; Sodium-coupled nucleoside transporter 2; Sodium/purine nucleoside co-transporter; SPNT; Solute carrier family 28 member 2 from Homo sapiens (Human) (see 4 papers)
TC 2.A.41.2.4 / O43868 Purine nucleoside, uridine, and 2'3'dideoxyinosine cladribrine:Na+ symporter, CNT2 (Na+/nucleoside = 1) from Homo sapiens (Human) (see 7 papers)
    24% identity, 92% coverage of query (112 bits)

CNT cation-coupled nucleoside transporter (H(+)/nucleoside symporter) from Candida albicans (see 5 papers)
    25% identity, 92% coverage of query (110 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory