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Searching for up to 100 curated homologs for WP_090446759.1 NCBI__GCF_900100495.1:WP_090446759.1 (262 a.a.)

Found high-coverage hits (≥70%) to 81 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

AO353_17230 Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas fluorescens FW300-N2E3
    68% identity, 98% coverage of query (369 bits)

pcaD / Q88N36 subunit of 3-oxoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 5 papers)
Q88N36 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Pseudomonas putida (see paper)
    68% identity, 99% coverage of query (345 bits)

2xuaH / Q13KT2 Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
    48% identity, 100% coverage of query (250 bits)

Q13KT2 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Paraburkholderia xenovorans (see 2 papers)
    48% identity, 100% coverage of query (250 bits)

catD / AAC46435.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 8 papers)
    48% identity, 98% coverage of query (239 bits)

catD / BAA75208.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see paper)
catD / BAC82535.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see 3 papers)
    49% identity, 98% coverage of query (236 bits)

pcaD / AAC37150.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 11 papers)
    43% identity, 96% coverage of query (209 bits)

pcaL / AAC38246.1 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Rhodococcus opacus (see paper)
    43% identity, 95% coverage of query (194 bits)

Q0SH24 3-oxoadipate enol-lactonase (EC 3.1.1.24); 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) from Rhodococcus jostii (see paper)
    43% identity, 95% coverage of query (189 bits)

pcaD / AAF34270.1 beta-ketoadipate enol-lactone hydrolase from Agrobacterium tumefaciens (see 2 papers)
    38% identity, 96% coverage of query (162 bits)

bioH / BAB39459.1 BioH from Kurthia sp. 538-KA26 (see paper)
    32% identity, 99% coverage of query (127 bits)

6eb3A Structural and enzymatic characterization of an esterase from a metagenomic library
    35% identity, 96% coverage of query (102 bits)

6eb3C Structural and enzymatic characterization of an esterase from a metagenomic library
    33% identity, 96% coverage of query (100 bits)

6eb3B Structural and enzymatic characterization of an esterase from a metagenomic library
    33% identity, 96% coverage of query (98.6 bits)

Build an alignment

Build an alignment for WP_090446759.1 and 14 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

4uhfA / A0A0M3KKY6 Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
    28% identity, 93% coverage of query (85.5 bits)

4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound)
    28% identity, 93% coverage of query (85.5 bits)

4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound)
    28% identity, 93% coverage of query (85.5 bits)

Q8KT44 carboxylesterase (EC 3.1.1.1) from Lacticaseibacillus casei (see paper)
    27% identity, 96% coverage of query (83.6 bits)

O73957 carboxylesterase (EC 3.1.1.1) from Sulfolobus acidocaldarius (see paper)
    26% identity, 95% coverage of query (76.3 bits)

PHAZ_PSEOL / P26495 Poly(3-hydroxyalkanoate) depolymerase; PHA depolymerase; ORF2; PHB depolymerase; EC 3.1.1.- from Pseudomonas oleovorans (see paper)
phaB / AAA25933.1 PHA-depolymerase from Pseudomonas oleovorans (see paper)
    26% identity, 91% coverage of query (75.1 bits)

Q5Y152 poly(3-hydroxyoctanoate) depolymerase (EC 3.1.1.76) from Pseudomonas putida (see paper)
    25% identity, 91% coverage of query (71.6 bits)

phaZ / Q8KQ22 intracellular poly(3-hydroxyoctanoate) depolymerase (EC 3.1.1.76) from Pseudomonas putida (see 6 papers)
    26% identity, 91% coverage of query (71.2 bits)

nahN / BAE92169.1 2-hydroxymuconic semialdehyde hydrolase NahN from Pseudomonas putida (see 2 papers)
    29% identity, 98% coverage of query (66.6 bits)

cmpF / CAB06612.1 2-hydroxymuconic semialdehyde hydrolase from Sphingomonas sp (see 2 papers)
    33% identity, 96% coverage of query (66.6 bits)

G3KFX4 2-hydroxymuconate-6-semialdehyde hydrolase (EC 3.7.1.9) from Pseudomonas sp. (see paper)
    30% identity, 93% coverage of query (66.2 bits)

Q6PLI2 poly(3-hydroxyoctanoate) depolymerase (EC 3.1.1.76) from Pseudomonas putida (see paper)
    26% identity, 91% coverage of query (65.5 bits)

RSBQ_BACSU / O07015 Sigma factor SigB regulation protein RsbQ from Bacillus subtilis (strain 168) (see 2 papers)
    23% identity, 89% coverage of query (65.1 bits)

EstN1 / K0IAM1 pimeloyl-[acyl-carrier protein] methyl ester esterase (EC 3.1.1.85) from Nitrososphaera gargensis (strain Ga9.2) (see paper)
    23% identity, 95% coverage of query (64.7 bits)

5h3hB Esterase (eaest) from exiguobacterium antarcticum (see paper)
    22% identity, 98% coverage of query (64.3 bits)

PFMAB_PESFW / W3XA95 Proline iminopeptidase PfmaB; PIP; Conidial pigment biosynthesis cluster protein B; EC 3.4.11.5 from Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) (see 2 papers)
    25% identity, 99% coverage of query (63.9 bits)

P96965 2-hydroxymuconate-6-semialdehyde hydrolase (EC 3.7.1.9) from Pseudomonas fluorescens (see 2 papers)
cumD / BAA12150.1 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase from Pseudomonas fluorescens (see paper)
    26% identity, 96% coverage of query (63.2 bits)

D14_ORYSJ / Q10QA5 Strigolactone esterase D14; Protein DWARF 14; Protein DWARF 88; Protein HIGH-TILLERING DWARF 2; EC 3.1.-.- from Oryza sativa subsp. japonica (Rice) (see 11 papers)
    26% identity, 89% coverage of query (63.2 bits)

5cbkA / A0A0M5I297 Crystal structure of the strigolactone receptor shhtl5 from striga hermonthica (see paper)
    20% identity, 86% coverage of query (63.2 bits)

6brtA / A0A0D9Z3K8 F-box protein cth with hydrolase (see paper)
    26% identity, 89% coverage of query (63.2 bits)

6ap8A Crystal structure of rice d14 bound to 2-(2-methyl-3-nitroanilino) benzoic acid
    26% identity, 89% coverage of query (63.2 bits)

5zhsA Crystal structure of osd14 in complex with covalently bound kk052
    26% identity, 89% coverage of query (63.2 bits)

5dj5A Crystal structure of rice dwarf14 in complex with synthetic strigolactone gr24
    26% identity, 89% coverage of query (63.2 bits)

4ihaA Crystal structure of rice dwarf14 (d14) in complex with a gr24 hydrolysis intermediate
    26% identity, 89% coverage of query (63.2 bits)

5zhtA Crystal structure of osd14 in complex with covalently bound kk073
    26% identity, 89% coverage of query (63.2 bits)

5zhrA Crystal structure of osd14 in complex with covalently bound kk094
    26% identity, 89% coverage of query (63.2 bits)

5yz7A Crystal structure of osd14 in complex with d-ring-opened 7'-carba-4bd
    26% identity, 89% coverage of query (63.2 bits)

5z7xA / A0A0M4AMQ0 Crystal structure of striga hermonthica htl4 (shhtl4) (see paper)
    22% identity, 86% coverage of query (62.8 bits)

hppC / AAB81313.1 2-hydroxy-6-ketonona-2,4-dienoate hydrolase from Rhodococcus globerulus (see paper)
    26% identity, 98% coverage of query (62.0 bits)

1iunB / P96965 Meta-cleavage product hydrolase from pseudomonas fluorescens ip01 (cumd) s103a mutant hexagonal (see paper)
    26% identity, 96% coverage of query (62.0 bits)

1ukaA Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with (s)-2-methylbutyrate
    26% identity, 96% coverage of query (62.0 bits)

1uk9A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with isovalerate
    26% identity, 96% coverage of query (62.0 bits)

1uk8A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with n-valerate
    26% identity, 96% coverage of query (62.0 bits)

1uk7A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with n-butyrate
    26% identity, 96% coverage of query (62.0 bits)

1iupA Meta-cleavage product hydrolase from pseudomonas fluorescens ip01 (cumd) s103a mutant complexed with isobutyrates
    26% identity, 96% coverage of query (62.0 bits)

8dvcA Receptor shhtl5 from striga hermonthica in complex with strigolactone agonist gr24
    20% identity, 86% coverage of query (61.6 bits)

2d0dA Crystal structure of a meta-cleavage product hydrolase (cumd) a129v mutant
    26% identity, 96% coverage of query (61.6 bits)

TGND_ACIAD / Q6F9F4 (E)-2-((N-methylformamido)methylene)succinate hydrolase; MFMS hydrolase; EC 3.5.1.- from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
    22% identity, 99% coverage of query (60.8 bits)

bpdF / AAB17100.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Rhodococcus sp. M5 (see paper)
    26% identity, 97% coverage of query (60.5 bits)

BPOC_MYCTU / P9WNH1 Putative non-heme bromoperoxidase BpoC from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
    25% identity, 96% coverage of query (60.1 bits)

3heaA The l29p/l124i mutation of pseudomonas fluorescens esterase
    25% identity, 97% coverage of query (59.7 bits)

RutD / b1009 putative aminoacrylate hydrolase RutD from Escherichia coli K-12 substr. MG1655 (see 2 papers)
RUTD_ECOLI / P75895 Putative carbamate hydrolase RutD; Aminohydrolase; EC 3.5.1.- from Escherichia coli (strain K12) (see 5 papers)
    25% identity, 90% coverage of query (59.3 bits)

dnrP / Q54809 rhodomycinone D methyl-esterase from Streptomyces peucetius (see 3 papers)
    24% identity, 91% coverage of query (59.3 bits)

dauP / Q55217 rhodomycinone D methylesterase from Streptomyces sp. (strain C5) (see 2 papers)
DNRP_STRS5 / Q55217 Rhodomycin D methylesterase DauP; 10-carbomethoxy-13-deoxycarminomycin esterase; 4-O-methylrhodomycin D methylesterase; EC 3.1.1.- from Streptomyces sp. (strain C5) (see paper)
    25% identity, 91% coverage of query (58.9 bits)

8pi1B / P22862 Bicyclic incypro pseudomonas fluorescens esterase (see paper)
    25% identity, 97% coverage of query (58.9 bits)

3hi4A Switching catalysis from hydrolysis to perhydrolysis in p. Fluorescens esterase
    25% identity, 97% coverage of query (58.9 bits)

ESTE_PSEFL / P22862 Arylesterase; Aryl-ester hydrolase; Carboxylic acid perhydrolase; PFE; Putative bromoperoxidase; EC 3.1.1.2; EC 1.-.-.- from Pseudomonas fluorescens (see 8 papers)
GI|951089 arylesterase; EC 1.-.-.-; EC 3.1.1.2 from Pseudomonas fluorescens (see 3 papers)
AAB60168.1 esterase from Pseudomonas fluorescens (see 2 papers)
    25% identity, 97% coverage of query (58.2 bits)

3ia2A Pseudomonas fluorescens esterase complexed to the r-enantiomer of a sulfonate transition state analog
    25% identity, 97% coverage of query (58.2 bits)

7snuA / A0A0M3PNA2 Crystal structure of shhtl7 from striga hermonthica in complex with strigolactone antagonist rg6 (see paper)
    21% identity, 93% coverage of query (56.2 bits)

7c8lA Hybrid designing of potent inhibitors of striga strigolactone receptor shhtl7
    21% identity, 93% coverage of query (56.2 bits)

5z7yA Crystal structure of striga hermonthica htl7 (shhtl7)
    21% identity, 93% coverage of query (56.2 bits)

RMS3_PEA / A0A109QYD3 Strigolactone esterase RMS3; Protein DWARF 14 homolog; PsD14; Protein RAMOSUS 3; EC 3.1.-.- from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see paper)
    21% identity, 89% coverage of query (55.8 bits)

B0M0H6 carboxylesterase (EC 3.1.1.1) from Serratia sp. (see paper)
    25% identity, 73% coverage of query (54.7 bits)

5z89A Structural basis for specific inhibition of highly sensitive shhtl7 receptor
    20% identity, 93% coverage of query (54.7 bits)

D14_ARATH / Q9SQR3 Strigolactone esterase D14; Protein DWARF 14; AtD14; EC 3.1.-.- from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
    21% identity, 87% coverage of query (54.3 bits)

6o5jA Crystal structure of dad2 bound to quinazolinone derivative
    22% identity, 89% coverage of query (53.9 bits)

6ap7A Crystal structure of dad2 in complex with 2-(2-methyl-3-nitroanilino) benzoic acid
    22% identity, 89% coverage of query (53.9 bits)

6ap6A / J9U5U9 Crystal structure of dad2 in complex with tolfenamic acid (see paper)
    22% identity, 89% coverage of query (53.9 bits)

5hzgA / Q9SQR3 The crystal structure of the strigolactone-induced atd14-d3-ask1 complex (see paper)
    23% identity, 76% coverage of query (53.5 bits)

5z7wB / A0A0M4AV81 Crystal structure of striga hermonthica htl1 (shhtl1) (see paper)
    24% identity, 85% coverage of query (52.8 bits)

7k38A Crystal structure of pisum sativum kai2 in complex with gr24-ent5ds product (see paper)
    23% identity, 88% coverage of query (52.8 bits)

P25026 Non-heme chloroperoxidase; Chloride peroxidase; Chloroperoxidase P; CPO-P; EC 1.11.1.- from Burkholderia pyrrocinia (Pseudomonas pyrrocinia)
    23% identity, 92% coverage of query (52.4 bits)

DAD2_PETHY / J9U5U9 Probable strigolactone esterase DAD2; Protein DECREASED APICAL DOMINANCE 2; EC 3.1.-.- from Petunia hybrida (Petunia) (see paper)
    22% identity, 89% coverage of query (52.4 bits)

5dnuA / A0A0M3PN85 Crystal structure of striga kai2-like protein in complex with karrikin (see paper)
    21% identity, 84% coverage of query (50.4 bits)

1q0zA Crystal structure of aclacinomycin methylesterase (rdmc) with bound product analogue, 10-decarboxymethylaclacinomycin a (dcma)
    24% identity, 73% coverage of query (49.7 bits)

1q0rA / Q54528 Crystal structure of aclacinomycin methylesterase (rdmc) with bound product analogue, 10-decarboxymethylaclacinomycin t (dcmat) (see paper)
    24% identity, 73% coverage of query (49.7 bits)

carC / Q9AQM4 2-hydroxy-6-oxo-6-(2'-aminophenyl)-hexa-2,4dienoate hydrolase monomer (EC 3.7.1.13) from Pseudomonas resinovorans (see paper)
CARC_PSERE / Q9AQM4 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase; HOPDA; EC 3.7.1.13 from Pseudomonas resinovorans (see 3 papers)
Q9AQM4 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase (EC 3.7.1.13) from Pseudomonas resinovorans (see paper)
carC / BAC41548.1 meta cleavage compound hydrolase from Pseudomonas resinovorans (see 9 papers)
    23% identity, 95% coverage of query (42.7 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory