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Searching for up to 100 curated homologs for WP_108402814.1 NCBI__GCF_003063475.1:WP_108402814.1 (302 a.a.)

Found high-coverage hits (≥70%) to 53 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

HMGCL / P35914 Hydroxymethylglutaryl-CoA lyase, mitochondrial (EC 4.1.3.4) from Homo sapiens (see 9 papers)
HMGCL_HUMAN / P35914 Hydroxymethylglutaryl-CoA lyase, mitochondrial; HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; EC 4.1.3.4 from Homo sapiens (Human) (see 18 papers)
P35914 hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) from Homo sapiens (see 4 papers)
    57% identity, 97% coverage of query (356 bits)

2cw6A / P35914 Crystal structure of human hmg-coa lyase: insights into catalysis and the molecular basis for hydroxymethylglutaric aciduria (see paper)
    57% identity, 97% coverage of query (355 bits)

3mp3B Crystal structure of human lyase in complex with inhibitor hg-coa
    57% identity, 97% coverage of query (355 bits)

HMGCL_RAT / P97519 Hydroxymethylglutaryl-CoA lyase, mitochondrial; HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; EC 4.1.3.4 from Rattus norvegicus (Rat) (see paper)
P97519 hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) from Rattus norvegicus (see paper)
    56% identity, 97% coverage of query (352 bits)

3mp5B Crystal structure of human lyase r41m in complex with hmg-coa
    56% identity, 97% coverage of query (352 bits)

HMGCL_MOUSE / P38060 Hydroxymethylglutaryl-CoA lyase, mitochondrial; HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; EC 4.1.3.4 from Mus musculus (Mouse) (see paper)
    56% identity, 97% coverage of query (347 bits)

HMGC2_RAT / D4A5C3 3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic; 3-hydroxy-3-methylglutaryl-CoA lyase-like protein 1; EC 4.1.3.4 from Rattus norvegicus (Rat) (see paper)
    54% identity, 99% coverage of query (343 bits)

HMGC2_HUMAN / Q8TB92 3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic; 3-hydroxy-3-methylglutaryl-CoA lyase-like protein 1; HMGCL-like 1; Endoplasmic reticulum 3-hydroxy-3-methylglutaryl-CoA lyase; er-cHL; EC 4.1.3.4 from Homo sapiens (Human) (see 2 papers)
    55% identity, 98% coverage of query (343 bits)

liuE / Q9I2A0 hydroxymethylglutaryl-CoA lyase subunit (EC 4.1.3.26; EC 4.1.3.4) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
LIUE_PSEAE / Q9I2A0 3-hydroxy-3-isohexenylglutaryl-CoA/hydroxy-methylglutaryl-CoA lyase; HIHG-CoA lyase; HMG-CoA lyase; (S)-3-hydroxy-3-methylglutaryl-CoA acetoacetate-lyase; 3-hydroxy-3-(4-methylpent-3-en-1-yl)glutaryl-CoA acetate-lyase; EC 4.1.3.26; EC 4.1.3.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
Q9I2A0 3-Hydroxy-3-isohexenylglutaryl-CoA lyase (EC 4.1.3.26) from Pseudomonas aeruginosa PAO1 (see paper)
    57% identity, 96% coverage of query (342 bits)

HMGCL_ARATH / O81027 Hydroxymethylglutaryl-CoA lyase, mitochondrial; HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; EC 4.1.3.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    53% identity, 99% coverage of query (322 bits)

1ydnA / Q8YEF2 Crystal structure of the hmg-coa lyase from brucella melitensis, northeast structural genomics target lr35. (see paper)
    53% identity, 90% coverage of query (289 bits)

SM_b21125 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) from Sinorhizobium meliloti 1021
    51% identity, 91% coverage of query (288 bits)

mvaB / P13703 hydroxymethylglutaryl-CoA lyase monomer (EC 4.1.3.4) from Pseudomonas mevalonii (see 8 papers)
mvaB / GB|AAA25895.1 hydroxymethylglutaryl-CoA lyase; EC 4.1.3.4 from Pseudomonas mevalonii (see paper)
    49% identity, 98% coverage of query (280 bits)

P13703 Hydroxymethylglutaryl-CoA lyase; HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; EC 4.1.3.4 from Pseudomonas mevalonii
    49% identity, 98% coverage of query (280 bits)

hlyA 3-hydroxy-3-methylglutaryl-coenzyme A lyase/3-methylglutaconyl-coenzyme A hydratase; EC 4.1.3.4; EC 4.2.1.18 from Emericella nidulans (see paper)
    45% identity, 94% coverage of query (250 bits)

HMGCL_BACSU / O34873 Hydroxymethylglutaryl-CoA lyase YngG; HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; EC 4.1.3.4 from Bacillus subtilis (strain 168) (see paper)
    43% identity, 95% coverage of query (248 bits)

6ndsA / A0A0D5YK08 Structure of an hmg-coa lyase from acenitobacter baumannii in complex with coenzyme a and 3-methylmalate
    36% identity, 94% coverage of query (191 bits)

CCL_CHLAA / A9WGE2 (R)-citramalyl-CoA lyase; EC 4.1.3.46 from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) (see paper)
    36% identity, 96% coverage of query (190 bits)

CA265_RS13115 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) from Pedobacter sp. GW460-11-11-14-LB5
    31% identity, 92% coverage of query (139 bits)

Build an alignment

Build an alignment for WP_108402814.1 and 19 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

LEU1_METJA / Q58595 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58595 2-isopropylmalate synthase (EC 2.3.3.13) from Methanocaldococcus jannaschii (see paper)
    26% identity, 97% coverage of query (68.6 bits)

hcs / O87198 homocitrate synthase (EC 2.3.3.14) from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
HOSA_THET2 / O87198 Homocitrate synthase; HCS; EC 2.3.3.14 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 3 papers)
    27% identity, 89% coverage of query (65.9 bits)

C7C437 homocitrate synthase (EC 2.3.3.14) from Aspergillus fumigatus (see paper)
    26% identity, 77% coverage of query (65.5 bits)

2zyfA Crystal structure of homocitrate synthase from thermus thermophilus complexed with magnesuim ion and alpha-ketoglutarate
    27% identity, 89% coverage of query (65.1 bits)

3ivtB / Q9Y823 Homocitrate synthase lys4 bound to 2-og (see paper)
    25% identity, 76% coverage of query (64.7 bits)

HOSM_SCHPO / Q9Y823 Homocitrate synthase, mitochondrial; HCS; EC 2.3.3.14 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
lys4 / RF|NP_596458.1 homocitrate synthase (predicted); EC 2.3.3.14 from Schizosaccharomyces pombe (see 2 papers)
    25% identity, 76% coverage of query (64.7 bits)

2ztjA Crystal structure of homocitrate synthase from thermus thermophilus complexed with alpha-ketoglutarate
    27% identity, 89% coverage of query (64.7 bits)

cimA / Q58787 (R)-citratemalate synthase subunit (EC 2.3.3.21) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see paper)
CIMA_METJA / Q58787 (R)-citramalate synthase CimA; EC 2.3.3.21 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58787 (R)-citramalate synthase (EC 2.3.3.21) from Methanocaldococcus jannaschii (see 2 papers)
    24% identity, 92% coverage of query (64.3 bits)

3a9iA / O87198 Crystal structure of homocitrate synthase from thermus thermophilus complexed with lys (see paper)
    27% identity, 89% coverage of query (63.9 bits)

LYS21 / Q12122 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 6 papers)
HOSM_YEAST / Q12122 Homocitrate synthase, mitochondrial; HCS; EC 2.3.3.14 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
Q12122 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (see paper)
    26% identity, 77% coverage of query (63.5 bits)

LYS20 / P48570 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 6 papers)
HOSC_YEAST / P48570 Homocitrate synthase, cytosolic isozyme; HCS; EC 2.3.3.14 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
P48570 homocitrate synthase (EC 2.3.3.14) from Saccharomyces cerevisiae (see 2 papers)
    26% identity, 76% coverage of query (63.2 bits)

LEU1_SULAC / Q4JA78 2-isopropylmalate synthase; IPMS; Alpha-isopropylmalate synthase; Alpha-IPM synthase; EC 2.3.3.13 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
    23% identity, 93% coverage of query (61.6 bits)

3mi3A Homocitrate synthase lys4 bound to lysine
    25% identity, 76% coverage of query (61.6 bits)

HOSA_SULAC / Q4J989 Homocitrate synthase; HCS; EC 2.3.3.14 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
Q4J989 homocitrate synthase (EC 2.3.3.14) from Sulfolobus acidocaldarius (see paper)
    22% identity, 78% coverage of query (61.2 bits)

6ktqA / Q4J989 Crystal structure of catalytic domain of homocitrate synthase from sulfolobus acidocaldarius (sahcs(dram)) in complex with alpha- ketoglutarate/zn2+/coa (see paper)
    22% identity, 78% coverage of query (60.1 bits)

3ivsA Homocitrate synthase lys4
    26% identity, 76% coverage of query (59.3 bits)

nifV / P05342 homocitrate synthase monomer (EC 2.3.3.14) from Azotobacter vinelandii (see paper)
NIFV_AZOVI / P05342 Homocitrate synthase; EC 2.3.3.14 from Azotobacter vinelandii (see paper)
    26% identity, 92% coverage of query (58.9 bits)

AKSA_METJA / Q57926 Homocitrate synthase AksA; (R)-homo(2)citrate synthase; (R)-homo(3)citrate synthase; EC 2.3.3.14; EC 2.3.3.- from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
    23% identity, 89% coverage of query (58.2 bits)

A0A0S6UXF5 homocitrate synthase (EC 2.3.3.14) from Bradyrhizobium sp. DOA9 (see paper)
    28% identity, 89% coverage of query (56.2 bits)

nahM / BAE92172.1 4-hydroxy-2-oxovalerate aldolase NahM from Pseudomonas putida (see 2 papers)
    27% identity, 91% coverage of query (55.8 bits)

HOSA_SULTO / Q971S5 Homocitrate synthase; HCS; EC 2.3.3.14 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
    24% identity, 77% coverage of query (55.5 bits)

M1U949 homocitrate synthase (EC 2.3.3.14) from Flammulina velutipes (see paper)
    26% identity, 76% coverage of query (54.7 bits)

LEU1_NEIMB / Q9JZG1 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Neisseria meningitidis serogroup B (strain ATCC BAA-335 / MC58) (see 2 papers)
Q9JZG1 2-isopropylmalate synthase (EC 2.3.3.13) from Neisseria meningitidis (see 2 papers)
    24% identity, 97% coverage of query (53.9 bits)

3rmjB / Q9JZG1 Crystal structure of truncated alpha-isopropylmalate synthase from neisseria meningitidis (see paper)
    24% identity, 95% coverage of query (53.9 bits)

LEU11_LEPIN / Q8F445 2-isopropylmalate synthase 1; Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1; EC 2.3.3.13 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see paper)
    24% identity, 97% coverage of query (51.6 bits)

MAM12_EUTJA / P0DO78 Methylthioalkylmalate synthase 1-2, chloroplastic; EjMAM1-2; EC 2.3.3.17 from Eutrema japonicum (Wasabi plant) (Eutrema wasabi) (see paper)
    23% identity, 85% coverage of query (50.8 bits)

Q72JC9 2-isopropylmalate synthase (EC 2.3.3.13) from Thermus thermophilus (see 2 papers)
    25% identity, 86% coverage of query (49.7 bits)

MAM3 / Q9FN52 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see paper)
MAM3_ARATH / Q9FN52 Methylthioalkylmalate synthase 3, chloroplastic; 2-isopropylmalate synthase 2; Methylthioalkylmalate synthase-like; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
Q9FN52 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana (see 4 papers)
    22% identity, 87% coverage of query (48.9 bits)

MAM11_EUTJA / P0DO77 Methylthioalkylmalate synthase 1-1, chloroplastic; EjMAM1-1; EC 2.3.3.17 from Eutrema japonicum (Wasabi plant) (Eutrema wasabi) (see paper)
    23% identity, 85% coverage of query (47.8 bits)

A0A0G2T6D7 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum pennellii (see paper)
    24% identity, 96% coverage of query (47.0 bits)

K4CJ56 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum lycopersicum (see paper)
    24% identity, 96% coverage of query (47.0 bits)

hsaF / Q0S815 4-hydroxy-2-oxohexanoate aldolase (EC 4.1.3.43) from Rhodococcus jostii (strain RHA1) (see paper)
    23% identity, 89% coverage of query (46.6 bits)

frbC / Q0ZQ46 2-phosphonomethylmalate synthase (EC 2.3.3.19) from Streptomyces rubellomurinus (strain ATCC 31215) (see 2 papers)
FRBC_STRR3 / Q0ZQ46 2-phosphonomethylmalate synthase; EC 2.3.3.19 from Streptomyces rubellomurinus (strain ATCC 31215) (see paper)
Q0ZQ46 2-phosphonomethylmalate synthase (EC 2.3.3.19) from Streptomyces rubellomurinus (see paper)
    25% identity, 92% coverage of query (46.2 bits)

LEU1_SALTY / P15875 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 3 papers)
    23% identity, 85% coverage of query (46.2 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory