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Searching for up to 100 curated homologs for WP_110206771.1 NCBI__GCF_003194585.1:WP_110206771.1 (260 a.a.)

Found high-coverage hits (≥70%) to 79 curated proteins.

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

Q0SH24 3-oxoadipate enol-lactonase (EC 3.1.1.24); 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) from Rhodococcus jostii (see paper)
    45% identity, 94% coverage of query (206 bits)

pcaL / AAC38246.1 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Rhodococcus opacus (see paper)
    45% identity, 94% coverage of query (204 bits)

catD / AAC46435.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 8 papers)
    43% identity, 92% coverage of query (198 bits)

AO353_17230 Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas fluorescens FW300-N2E3
    43% identity, 94% coverage of query (189 bits)

pcaD / Q88N36 subunit of 3-oxoadipate enol-lactone hydrolase (EC 3.1.1.24) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see 5 papers)
Q88N36 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Pseudomonas putida (see paper)
    40% identity, 98% coverage of query (180 bits)

Q13KT2 3-oxoadipate enol-lactonase (EC 3.1.1.24) from Paraburkholderia xenovorans (see 2 papers)
    40% identity, 92% coverage of query (174 bits)

2xuaH / Q13KT2 Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
    40% identity, 92% coverage of query (173 bits)

catD / BAA75208.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see paper)
catD / BAC82535.1 b-ketoadipate enol-lactone hydrolase from Frateuria sp. ANA-18 (see 3 papers)
    40% identity, 92% coverage of query (172 bits)

pcaD / AAC37150.1 beta-ketoadipate enol-lactone hydrolase from Acinetobacter baylyi (see 11 papers)
    36% identity, 91% coverage of query (161 bits)

pcaD / AAF34270.1 beta-ketoadipate enol-lactone hydrolase from Agrobacterium tumefaciens (see 2 papers)
    35% identity, 87% coverage of query (140 bits)

4uhfA / A0A0M3KKY6 Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
    33% identity, 89% coverage of query (112 bits)

4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound)
    33% identity, 89% coverage of query (112 bits)

4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound)
    33% identity, 89% coverage of query (112 bits)

Build an alignment

Build an alignment for WP_110206771.1 and 13 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

bioH / BAB39459.1 BioH from Kurthia sp. 538-KA26 (see paper)
    29% identity, 90% coverage of query (93.2 bits)

O73957 carboxylesterase (EC 3.1.1.1) from Sulfolobus acidocaldarius (see paper)
    30% identity, 80% coverage of query (85.5 bits)

6eb3B Structural and enzymatic characterization of an esterase from a metagenomic library
    30% identity, 83% coverage of query (76.6 bits)

6eb3A Structural and enzymatic characterization of an esterase from a metagenomic library
    29% identity, 83% coverage of query (75.9 bits)

6eb3C Structural and enzymatic characterization of an esterase from a metagenomic library
    29% identity, 83% coverage of query (75.1 bits)

TGND_ACIAD / Q6F9F4 (E)-2-((N-methylformamido)methylene)succinate hydrolase; MFMS hydrolase; EC 3.5.1.- from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see paper)
    26% identity, 92% coverage of query (74.7 bits)

Q8KT44 carboxylesterase (EC 3.1.1.1) from Lacticaseibacillus casei (see paper)
    25% identity, 92% coverage of query (69.7 bits)

YBFK_BACSU / O31452 Carboxylesterase YbfK; EC 3.1.1.1 from Bacillus subtilis (strain 168) (see paper)
    27% identity, 94% coverage of query (64.7 bits)

EstN1 / K0IAM1 pimeloyl-[acyl-carrier protein] methyl ester esterase (EC 3.1.1.85) from Nitrososphaera gargensis (strain Ga9.2) (see paper)
    24% identity, 93% coverage of query (64.3 bits)

3hi4A Switching catalysis from hydrolysis to perhydrolysis in p. Fluorescens esterase
    28% identity, 86% coverage of query (62.8 bits)

3heaA The l29p/l124i mutation of pseudomonas fluorescens esterase
    28% identity, 86% coverage of query (62.8 bits)

3ia2A Pseudomonas fluorescens esterase complexed to the r-enantiomer of a sulfonate transition state analog
    28% identity, 86% coverage of query (62.8 bits)

ESTE_PSEFL / P22862 Arylesterase; Aryl-ester hydrolase; Carboxylic acid perhydrolase; PFE; Putative bromoperoxidase; EC 3.1.1.2; EC 1.-.-.- from Pseudomonas fluorescens (see 8 papers)
GI|951089 arylesterase; EC 1.-.-.-; EC 3.1.1.2 from Pseudomonas fluorescens (see 3 papers)
AAB60168.1 esterase from Pseudomonas fluorescens (see 2 papers)
    28% identity, 86% coverage of query (62.4 bits)

4lxhA Crystal structure of the s105a mutant of a carbon-carbon bond hydrolase, dxnb2 from sphingomonas wittichii rw1, in complex with 3- cl hopda (see paper)
    26% identity, 87% coverage of query (62.4 bits)

bphD / BAA25612.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Rhodococcus erythropolis (see paper)
    29% identity, 80% coverage of query (62.4 bits)

4lyeA Crystal structure of the s105a mutant of a c-c hydrolase, dxnb2 from sphingomonas wittichii rw1, in complex with substrate hopda
    26% identity, 87% coverage of query (62.4 bits)

4lxiA Crystal structure of the s105a mutant of a carbon-carbon bond hydrolase, dxnb2 from sphingomonas wittichii rw1, in complex with 5, 8-dif hopda
    26% identity, 87% coverage of query (62.4 bits)

ABHDB_BOVIN / Q3SZ73 sn-1-specific diacylglycerol lipase ABHD11; Alpha/beta hydrolase domain-containing protein 11; Abhydrolase domain-containing protein 11; EC 3.1.1.116 from Bos taurus (Bovine) (see paper)
    25% identity, 96% coverage of query (61.6 bits)

NAP_BACSU / P96688 Uncharacterized carboxylesterase nap; EC 3.1.1.1 from Bacillus subtilis (strain 168)
    24% identity, 92% coverage of query (61.2 bits)

8pi1B / P22862 Bicyclic incypro pseudomonas fluorescens esterase (see paper)
    28% identity, 86% coverage of query (61.2 bits)

RutD / b1009 putative aminoacrylate hydrolase RutD from Escherichia coli K-12 substr. MG1655 (see 2 papers)
RUTD_ECOLI / P75895 Putative carbamate hydrolase RutD; Aminohydrolase; EC 3.5.1.- from Escherichia coli (strain K12) (see 5 papers)
    27% identity, 91% coverage of query (61.2 bits)

D0EPY0 carboxylesterase (EC 3.1.1.1) from Bacillus subtilis (see paper)
    24% identity, 92% coverage of query (61.2 bits)

4ccwA / Q59248 Crystal structure of naproxen esterase (carboxylesterase np) from bacillus subtilis (see paper)
    24% identity, 92% coverage of query (60.5 bits)

flnE / BAC75995.1 meta cleavage compound hydrolase from Terrabacter sp. DBF63 (see paper)
    27% identity, 86% coverage of query (60.1 bits)

Q3HWU8 carboxylesterase (EC 3.1.1.1) from Pseudomonas putida (see paper)
    27% identity, 75% coverage of query (57.8 bits)

AGH13449.1 hybrid C-C meta-cleavage hydrolase-carboxylesterase from Cycloclasticus zancles (see paper)
    24% identity, 85% coverage of query (57.8 bits)

dauP / Q55217 rhodomycinone D methylesterase from Streptomyces sp. (strain C5) (see 2 papers)
DNRP_STRS5 / Q55217 Rhodomycin D methylesterase DauP; 10-carbomethoxy-13-deoxycarminomycin esterase; 4-O-methylrhodomycin D methylesterase; EC 3.1.1.- from Streptomyces sp. (strain C5) (see paper)
    27% identity, 92% coverage of query (57.0 bits)

ABHDB_HUMAN / Q8NFV4 sn-1-specific diacylglycerol lipase ABHD11; Alpha/beta hydrolase domain-containing protein 11; Abhydrolase domain-containing protein 11; Williams-Beuren syndrome chromosomal region 21 protein; EC 3.1.1.116 from Homo sapiens (Human) (see 3 papers)
    25% identity, 96% coverage of query (56.2 bits)

dnrP / Q54809 rhodomycinone D methyl-esterase from Streptomyces peucetius (see 3 papers)
    25% identity, 92% coverage of query (55.5 bits)

ABHDB_MOUSE / Q8K4F5 sn-1-specific diacylglycerol lipase ABHD11; Alpha/beta hydrolase domain-containing protein 11; Abhydrolase domain-containing protein 11; Williams-Beuren syndrome chromosomal region 21 protein homolog; EC 3.1.1.116 from Mus musculus (Mouse) (see 3 papers)
    27% identity, 96% coverage of query (54.7 bits)

PHAZ_PSEOL / P26495 Poly(3-hydroxyalkanoate) depolymerase; PHA depolymerase; ORF2; PHB depolymerase; EC 3.1.1.- from Pseudomonas oleovorans (see paper)
phaB / AAA25933.1 PHA-depolymerase from Pseudomonas oleovorans (see paper)
    25% identity, 94% coverage of query (53.9 bits)

bpdF / AAB17100.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase from Rhodococcus sp. M5 (see paper)
    26% identity, 92% coverage of query (53.1 bits)

picE / A0A1X9WE55 N-formylmaleamate deformylase (EC 3.5.1.106) from Alcaligenes faecalis (see paper)
    25% identity, 85% coverage of query (52.8 bits)

5dnuA / A0A0M3PN85 Crystal structure of striga kai2-like protein in complex with karrikin (see paper)
    24% identity, 90% coverage of query (52.4 bits)

rdmC / Q54528 aclacinomycin methylesterase (EC 3.1.1.95) from Streptomyces purpurascens (see 2 papers)
RDMC_STREF / Q54528 Aclacinomycin methylesterase RdmC; EC 3.1.1.95 from Streptomyces purpurascens (see 3 papers)
Q54528 aclacinomycin methylesterase (EC 3.1.1.95) from Streptomyces purpurascens (see paper)
    26% identity, 81% coverage of query (52.0 bits)

1q0rA / Q54528 Crystal structure of aclacinomycin methylesterase (rdmc) with bound product analogue, 10-decarboxymethylaclacinomycin t (dcmat) (see paper)
    26% identity, 81% coverage of query (52.0 bits)

1q0zA Crystal structure of aclacinomycin methylesterase (rdmc) with bound product analogue, 10-decarboxymethylaclacinomycin a (dcma)
    26% identity, 81% coverage of query (52.0 bits)

8hgwA / Q2MHH5 Crystal structure of mehph in complex with mbp (see paper)
    23% identity, 85% coverage of query (50.8 bits)

4ihaA Crystal structure of rice dwarf14 (d14) in complex with a gr24 hydrolysis intermediate
    26% identity, 77% coverage of query (49.3 bits)

6ap8A Crystal structure of rice d14 bound to 2-(2-methyl-3-nitroanilino) benzoic acid
    25% identity, 77% coverage of query (49.3 bits)

5dj5A Crystal structure of rice dwarf14 in complex with synthetic strigolactone gr24
    25% identity, 77% coverage of query (49.3 bits)

5zhsA Crystal structure of osd14 in complex with covalently bound kk052
    25% identity, 77% coverage of query (49.3 bits)

5zhtA Crystal structure of osd14 in complex with covalently bound kk073
    25% identity, 77% coverage of query (48.9 bits)

5zhrA Crystal structure of osd14 in complex with covalently bound kk094
    25% identity, 77% coverage of query (48.9 bits)

5yz7A Crystal structure of osd14 in complex with d-ring-opened 7'-carba-4bd
    25% identity, 77% coverage of query (48.9 bits)

6brtA / A0A0D9Z3K8 F-box protein cth with hydrolase (see paper)
    25% identity, 77% coverage of query (48.9 bits)

8y9oA Crystal structure of atkai2 in complex with kk181n1
    22% identity, 90% coverage of query (48.9 bits)

4jymA / Q9SZU7 Crystal structure of kai2 in complex with 3-methyl-2h-furo[2,3- c]pyran-2-one (see paper)
    22% identity, 90% coverage of query (48.9 bits)

D14_ORYSJ / Q10QA5 Strigolactone esterase D14; Protein DWARF 14; Protein DWARF 88; Protein HIGH-TILLERING DWARF 2; EC 3.1.-.- from Oryza sativa subsp. japonica (Rice) (see 11 papers)
    25% identity, 77% coverage of query (48.5 bits)

KAI2_ARATH / Q9SZU7 Probable esterase KAI2; Protein DWARF-14-like; Protein D14-like; Protein HYPOSENSITIVE TO LIGHT; Protein KARRIKIN INSENSITIVE 2 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
    22% identity, 90% coverage of query (48.5 bits)

2d0dA Crystal structure of a meta-cleavage product hydrolase (cumd) a129v mutant
    23% identity, 72% coverage of query (48.1 bits)

RSBQ_BACSU / O07015 Sigma factor SigB regulation protein RsbQ from Bacillus subtilis (strain 168) (see 2 papers)
    23% identity, 91% coverage of query (47.4 bits)

1iunB / P96965 Meta-cleavage product hydrolase from pseudomonas fluorescens ip01 (cumd) s103a mutant hexagonal (see paper)
    22% identity, 72% coverage of query (47.0 bits)

1ukaA Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with (s)-2-methylbutyrate
    22% identity, 72% coverage of query (47.0 bits)

1uk9A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with isovalerate
    22% identity, 72% coverage of query (47.0 bits)

1uk8A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with n-valerate
    22% identity, 72% coverage of query (47.0 bits)

1uk7A Crystal structure of a meta-cleavage product hydrolase (cumd) complexed with n-butyrate
    22% identity, 72% coverage of query (47.0 bits)

1iupA Meta-cleavage product hydrolase from pseudomonas fluorescens ip01 (cumd) s103a mutant complexed with isobutyrates
    22% identity, 72% coverage of query (47.0 bits)

8agpAAA Alpha/beta epoxide hydrolase
    24% identity, 87% coverage of query (45.8 bits)

5ng7B Novel epoxide hydrolases belonging to the alpha/beta hydrolases superfamily in metagenomes from hot environments
    24% identity, 87% coverage of query (45.8 bits)

8agsAAA / A0A1U9WZ52 8agsAAA
    24% identity, 87% coverage of query (45.8 bits)

8agnAAA Alpha/beta epoxide hydrolase
    24% identity, 87% coverage of query (45.4 bits)

8agmAAA Alpha/beta epoxide hydrolase
    24% identity, 87% coverage of query (45.4 bits)

aamsh1 / Q988D4 2-(acetamidomethylene)succinate hydrolase monomer (EC 3.5.1.29) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (see 7 papers)
AAMHY_RHILO / Q988D4 2-(acetamidomethylene)succinate hydrolase; alpha-(N-acetylaminomethylene)succinic acid amidohydrolase; AAMS amidohydrolase; EC 3.5.1.29 from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) (see 2 papers)
Q988D4 2-(acetamidomethylene)succinate hydrolase (EC 3.5.1.29) from Mesorhizobium loti (see 2 papers)
    27% identity, 82% coverage of query (45.1 bits)

4k2aC / E2RV62 Crystal structure of haloalkane dehalogenase dbea from bradyrhizobium elkani usda94 (see paper)
    22% identity, 94% coverage of query (42.7 bits)

5w15D / B1YPY7 Crystal structure of an alpha/beta hydrolase fold protein from burkholderia ambifaria.
    24% identity, 91% coverage of query (42.7 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory