Align Probable galactose dehydrogenase GalD; EC 1.1.1.- (characterized)
to candidate Pf1N1B4_4943 Oxidoreductase, short chain dehydrogenase/reductase family
Query= SwissProt::Q92RN6 (256 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4943 Length = 263 Score = 117 bits (293), Expect = 2e-31 Identities = 79/245 (32%), Positives = 121/245 (49%), Gaps = 4/245 (1%) Query: 11 LRDRGVLVTGGGSGIGAALVEAFARQGARVAFVDIAAESSLALCEKVAAQTGQAPHFIQA 70 L + L+TG SGIG A+ AFAR+GA VA + + Q G+ + Sbjct: 18 LEGKIALITGADSGIGRAVAIAFAREGADVAIAYLNETEDAQETARWVEQAGRQSLLLPG 77 Query: 71 DLRNVEAVRAAADEAVAKLGSVRVLVNNAA-RDDRQALEAVTEESWDESLSVNLRHLFFM 129 D+ +A D+ + + G + +LVNNAA + + LE + +E W + VN+ +F + Sbjct: 78 DVAQKAHCQALVDKTIERFGRIDILVNNAAFQMTHENLEDIPDEEWVMTFDVNITAMFRL 137 Query: 130 CQAVAPHMQRQGGGSIVNFSSIAFLLNMPEIPAYSTAKAGIIGLTKSLAGKLGPDNIRVN 189 CQA HM + G SI+N SS+ + P + AY+T K I + LA LGP NIRVN Sbjct: 138 CQAALKHM--KPGSSIINTSSVNSDMPKPTLLAYATTKGAIANFSAGLAQMLGPKNIRVN 195 Query: 190 AILPGMIVTERQRRLWLTEESIARMQERQCLKRMLVADDLVGPCLFLASDSSAAMTAQAM 249 ++ PG I T + EE + + L R ++ + LASD ++ +T Q Sbjct: 196 SVAPGPIWTPLIVST-MPEEDVQNFGAQTPLGRPGQPVEVAPIYVLLASDEASYITGQRY 254 Query: 250 IIDGG 254 + GG Sbjct: 255 GVTGG 259 Lambda K H 0.321 0.133 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 140 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 256 Length of database: 263 Length adjustment: 24 Effective length of query: 232 Effective length of database: 239 Effective search space: 55448 Effective search space used: 55448 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory