Alignments for a candidate for metX in Magnetospirillum magneticum AMB-1

GapMind for Amino acid biosynthesis

Alignments for a candidate for metX in Magnetospirillum magneticum AMB-1

Align Homoserine O-acetyltransferase; HAT; Homoserine transacetylase; HTA; EC 2.3.1.31 (characterized)
to candidate WP_011386326.1 AMB_RS20100 homoserine O-acetyltransferase

Query= SwissProt::Q9AAS1
         (382 letters)



>NCBI__GCF_000009985.1:WP_011386326.1
          Length = 393

 Score =  521 bits (1341), Expect = e-152
 Identities = 253/363 (69%), Positives = 293/363 (80%), Gaps = 2/363 (0%)

Query: 20  NEPLRLDSGGVIEGLEIAYQTYGQLNADKSNAVLICHALTGDQHVASPHPTTGKPGWWQR 79
           + P+RLD G  +  +++AYQTYG+LNADKSNA+LICHALTGD +VA PHP T KPGWW  
Sbjct: 24  DRPIRLDCGVELGPIQVAYQTYGRLNADKSNAILICHALTGDHYVADPHPITAKPGWWHE 83

Query: 80  LVGPGKPLDPARHFIICSNVIGGCMGSTGPASINPATGKTYGLSFPVITIADMVRAQAML 139
           LVGPG+  D  R+F+ICSNV+GGCMG+TGP   NPATG+ +GL FPVITI DMV+ QA L
Sbjct: 84  LVGPGRVFDTDRYFLICSNVLGGCMGTTGPVDENPATGQAWGLDFPVITIGDMVKVQARL 143

Query: 140 VSALGVETLFAVVGGSMGGMQVQQWAVDYPERMFSAVVLASASRHSAQNIAFHEVGRQAI 199
           V  LG++ LF VVGGSMGGMQV +WA  YPER+FSA+ +ASA+RHSAQNIAFHEVGRQAI
Sbjct: 144 VDHLGIDQLFCVVGGSMGGMQVLKWAQVYPERVFSAIPIASAARHSAQNIAFHEVGRQAI 203

Query: 200 MADPDWRGGAYAEHGVRPEKGLAVARMAAHITYLSEPALQRKFGRELQ-RDGLSWGFDAD 258
           MADPDW  G Y + G RP +GLAVARMAAHITYLSEPAL +KFGR LQ RD +++GFDAD
Sbjct: 204 MADPDWCEGNYLQEGKRPHRGLAVARMAAHITYLSEPALHQKFGRNLQNRDTVTYGFDAD 263

Query: 259 FQVESYLRHQGSSFVDRFDANSYLYITRAMDYFDIAASHGGVLAKAFTRARNVRFCVLSF 318
           FQVESYLRHQG++FVDRFDANSYLYITRAMDYFD+AA  GGVLA AF R    RFCV+SF
Sbjct: 264 FQVESYLRHQGNTFVDRFDANSYLYITRAMDYFDLAAESGGVLANAF-RGTRTRFCVVSF 322

Query: 319 SSDWLYPTAENRHLVRALTAAGARAAFAEIESDKGHDAFLLDEPVMDAALEGFLASAERD 378
           +SDWL+PT E+R +V AL A  A  +F EI+SDKGHDAFLLDEP   A L GFL  A   
Sbjct: 323 TSDWLFPTPESRAVVHALNAVAANVSFVEIKSDKGHDAFLLDEPEFHATLTGFLEGAATH 382

Query: 379 RGL 381
           RGL
Sbjct: 383 RGL 385


Lambda     K      H
   0.321    0.135    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 564
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 393
Length adjustment: 30
Effective length of query: 352
Effective length of database: 363
Effective search space:   127776
Effective search space used:   127776
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

Align candidate WP_011386326.1 AMB_RS20100 (homoserine O-acetyltransferase)
to HMM TIGR01392 (metX: homoserine O-acetyltransferase (EC 2.3.1.31))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01392.hmm
# target sequence database:        /tmp/gapView.3947.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01392  [M=351]
Accession:   TIGR01392
Description: homoserO_Ac_trn: homoserine O-acetyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.8e-149  482.4   0.0   4.4e-149  482.3   0.0    1.0  1  lcl|NCBI__GCF_000009985.1:WP_011386326.1  AMB_RS20100 homoserine O-acetylt


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000009985.1:WP_011386326.1  AMB_RS20100 homoserine O-acetyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  482.3   0.0  4.4e-149  4.4e-149       3     350 ..      25     376 ..      23     377 .. 0.98

  Alignments for each domain:
  == domain 1  score: 482.3 bits;  conditional E-value: 4.4e-149
                                 TIGR01392   3 eeltlesGevlsevevayktyGtlnaerdNavlvcHaltgsahvagkadeedk.GWWdellGpgraldt 70 
                                               +++ l++G +l  ++vay+tyG+lna+++Na+l+cHaltg++ va+ +  ++k GWW+el+Gpgr +dt
  lcl|NCBI__GCF_000009985.1:WP_011386326.1  25 RPIRLDCGVELGPIQVAYQTYGRLNADKSNAILICHALTGDHYVADPHPITAKpGWWHELVGPGRVFDT 93 
                                               6789******************************************99988778*************** PP

                                 TIGR01392  71 sryfvvclNvlGsckGstgPlsinpetgkpygaefPlvtirDlvkaqkalldsLgveklaavvGgSlGG 139
                                               +ryf++c+NvlG+c G+tgP++ np+tg+ +g +fP++ti D+vk+q++l+d+Lg+++l++vvGgS+GG
  lcl|NCBI__GCF_000009985.1:WP_011386326.1  94 DRYFLICSNVLGGCMGTTGPVDENPATGQAWGLDFPVITIGDMVKVQARLVDHLGIDQLFCVVGGSMGG 162
                                               ********************************************************************* PP

                                 TIGR01392 140 mqalewalsypervkkivvlatsarasaqaiafnevqrqailsDpeyndGeyaeee.qPekGLalARml 207
                                               mq+l+wa  yperv +++++a++ar+saq+iaf+ev rqai++Dp++ +G+y +e+ +P++GLa+ARm 
  lcl|NCBI__GCF_000009985.1:WP_011386326.1 163 MQVLKWAQVYPERVFSAIPIASAARHSAQNIAFHEVGRQAIMADPDWCEGNYLQEGkRPHRGLAVARMA 231
                                               ******************************************************999************ PP

                                 TIGR01392 208 alltYrseesleerfgreakseeslassleeefsvesylryqgkkfverFdAnsYllltkaldthdlar 276
                                               a++tY+se +l+++fgr+ ++++++++ ++++f+vesylr+qg++fv+rFdAnsYl++t+a+d++dla+
  lcl|NCBI__GCF_000009985.1:WP_011386326.1 232 AHITYLSEPALHQKFGRNLQNRDTVTYGFDADFQVESYLRHQGNTFVDRFDANSYLYITRAMDYFDLAA 300
                                               ********************************************************************* PP

                                 TIGR01392 277 grrdslkealkkikapvlvvgiesDllftleeqeelakalkaakle..yaeieseeGHDaFllekekve 343
                                               +  + l++a++ ++++++vv+++sD+lf++ e++ +++al+a++++  ++ei+s++GHDaFll++ +++
  lcl|NCBI__GCF_000009985.1:WP_011386326.1 301 ESGGVLANAFRGTRTRFCVVSFTSDWLFPTPESRAVVHALNAVAANvsFVEIKSDKGHDAFLLDEPEFH 369
                                               *999*************************************9998888********************* PP

                                 TIGR01392 344 elirefl 350
                                               ++++ fl
  lcl|NCBI__GCF_000009985.1:WP_011386326.1 370 ATLTGFL 376
                                               9999987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (351 nodes)
Target sequences:                          1  (393 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 9.86
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis