Alignments for a candidate for hisF in Bacteroides thetaiotaomicron VPI-5482

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisF in Bacteroides thetaiotaomicron VPI-5482

Align imidazole glycerol-phosphate synthase (subunit 2/2) (EC 4.3.2.10) (characterized)
to candidate 350906 BT1378 imidazole glycerol phosphate synthase subunit hisF (NCBI ptt file)

Query= BRENDA::Q9X0C6
         (253 letters)



>FitnessBrowser__Btheta:350906
          Length = 251

 Score =  276 bits (706), Expect = 3e-79
 Identities = 142/250 (56%), Positives = 179/250 (71%), Gaps = 1/250 (0%)

Query: 1   MLAKRIIACLDVKDGRVVKGTNFENLRDSGDPVELGKFYSEIGIDELVFLDITASVEKRK 60
           MLAKRII CLD+KDG+ VKGTNF NLR +GDPVELG+ YSE G DELVFLDITAS E RK
Sbjct: 1   MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRAYSEQGADELVFLDITASHEGRK 60

Query: 61  TMLELVEKVAEQIDIPFTVGGGIHDFETASELILRGADKVSINTAAVENPSLITQIAQTF 120
           T  ELV+++A  I+IPFTVGGGI++      L+  GADK+SIN++A+ NP LI  IA+ F
Sbjct: 61  TFTELVKRIAANINIPFTVGGGINELSDVDRLLNAGADKISINSSAIRNPQLIDDIAKNF 120

Query: 121 GSQAVVVAIDAKRVDGEFMVFTYSGKKNTGILLRDWVVEVEKRGAGEILLTSIDRDGTKS 180
           GSQ  V+A+DAK+ +  +  +   G+  T   L  W  E ++RGAGEIL TS++ DG K+
Sbjct: 121 GSQVCVLAVDAKQTENGWKCYLNGGRIETDKELLAWTKEAQERGAGEILFTSMNHDGVKT 180

Query: 181 GYDTEMIRFVRPLTTLPIIASGGAGKMEHFLEAFLAG-ADAALAASVFHFREIDVRELKE 239
           GY  E +  +    ++P+IASGGAG  EHF +AFL G ADAALAASVFHF EI + ELK 
Sbjct: 181 GYANEALASLADQLSIPVIASGGAGLKEHFRDAFLVGKADAALAASVFHFGEIKIPELKS 240

Query: 240 YLKKHGVNVR 249
           YL   G+ +R
Sbjct: 241 YLCGEGITIR 250


Lambda     K      H
   0.320    0.138    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 211
Number of extensions: 7
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 253
Length of database: 251
Length adjustment: 24
Effective length of query: 229
Effective length of database: 227
Effective search space:    51983
Effective search space used:    51983
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

Align candidate 350906 BT1378 (imidazole glycerol phosphate synthase subunit hisF (NCBI ptt file))
to HMM TIGR00735 (hisF: imidazoleglycerol phosphate synthase, cyclase subunit)

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00735.hmm
# target sequence database:        /tmp/gapView.1373.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00735  [M=254]
Accession:   TIGR00735
Description: hisF: imidazoleglycerol phosphate synthase, cyclase subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                          Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                          -----------
   3.8e-111  356.4   0.1   4.2e-111  356.2   0.1    1.0  1  lcl|FitnessBrowser__Btheta:350906  BT1378 imidazole glycerol phosph


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Btheta:350906  BT1378 imidazole glycerol phosphate synthase subunit hisF (NCBI ptt file)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  356.2   0.1  4.2e-111  4.2e-111       1     254 []       1     250 [.       1     250 [. 0.99

  Alignments for each domain:
  == domain 1  score: 356.2 bits;  conditional E-value: 4.2e-111
                          TIGR00735   1 mlakriipCLdvkdgrvvkGvqfknlrdaGdpvelakkydeeGadelvflditassekretmlevvervaekvfiP 76 
                                        mlakriipCLd+kdg+ vkG++f nlr+aGdpvel ++y+e+Gadelvflditas+e+r+t +e+v+r+a ++ iP
  lcl|FitnessBrowser__Btheta:350906   1 MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRAYSEQGADELVFLDITASHEGRKTFTELVKRIAANINIP 76 
                                        8*************************************************************************** PP

                          TIGR00735  77 ltvgGGiksiedvkkllraGadkvsintaavkapelikeladrfGsqaivvaidakreaeneeakyevtikgGres 152
                                        +tvgGGi++++dv++ll+aGadk+sin++a+++p+li ++a+ fGsq++v+a+dak++++     ++++ +gGr +
  lcl|FitnessBrowser__Btheta:350906  77 FTVGGGINELSDVDRLLNAGADKISINSSAIRNPQLIDDIAKNFGSQVCVLAVDAKQTEN----GWKCYLNGGRIE 148
                                        *******************************************************98875....6*********** PP

                          TIGR00735 153 tdldvvewakeveelGaGeilltsmdkdGtksGydlellkkvkeavkiPviasgGaGkaehleeaflkgkadaaLa 228
                                        td ++++w+ke++e+GaGeil+tsm++dG+k+Gy  e l +++++++iPviasgGaG +eh+++afl gkadaaLa
  lcl|FitnessBrowser__Btheta:350906 149 TDKELLAWTKEAQERGAGEILFTSMNHDGVKTGYANEALASLADQLSIPVIASGGAGLKEHFRDAFLVGKADAALA 224
                                        **************************************************************************** PP

                          TIGR00735 229 asvfhkreltieevkeylaergvkvr 254
                                        asvfh++e++i e+k+yl  +g+++r
  lcl|FitnessBrowser__Btheta:350906 225 ASVFHFGEIKIPELKSYLCGEGITIR 250
                                        ************************98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (254 nodes)
Target sequences:                          1  (251 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 10.32
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis