Align argininosuccinate lyase (EC 4.3.2.1) (characterized)
to candidate 3609679 Dshi_3062 argininosuccinate lyase (RefSeq)
Query= BRENDA::Q8DVX5 (460 letters) >FitnessBrowser__Dino:3609679 Length = 463 Score = 438 bits (1127), Expect = e-127 Identities = 232/459 (50%), Positives = 301/459 (65%), Gaps = 1/459 (0%) Query: 3 TKNHKLWGGRFEAGLAQWVEEFGASISFDQKLAEFDLKGSIAHVTMLGEKGIISQEDAAT 62 TK++ +WGGRF AG +E ASI FD++LA D+ GS AH ML ++GI+S +DA Sbjct: 4 TKSNAMWGGRFAAGPDAIMEAINASIGFDRRLARQDIDGSRAHAAMLAQQGILSSKDAEA 63 Query: 63 IKAGLEDLLEEYKAGQLKFDVSNEDIHMNMESLLTAKIGPVAGKLHTARSRNDQVATDMH 122 I+ GL +L E + GQ F + EDIHMN+E+ L IG AG+LHT RSRNDQVATD Sbjct: 64 IREGLLTVLSEIETGQFAFSAALEDIHMNVEARLKELIGEPAGRLHTGRSRNDQVATDFK 123 Query: 123 LYLKAKLDEVIEKLANLRTVLVDLADKHVHTIMPGYTHLQHAQPISFGHHLMAYYNMFTR 182 L+++ +LD L L L+ A+ +MPG+THLQ AQP+++GHH+MAY MF R Sbjct: 124 LWVRDQLDAADAGLLALLRALLAQAEAGADWVMPGFTHLQTAQPVTWGHHMMAYVEMFAR 183 Query: 183 DSERFIFNVKHTDLSPLGAAALAGTTFPIDREMTAQLMGFAEPYSNSLDAVSDRDFILEF 242 D R + PLGAAALAGT+FP+DR+ TAQ +GF P +NSLDAVSDRDF LEF Sbjct: 184 DRGRMQDARARMNECPLGAAALAGTSFPLDRDATAQALGFDRPAANSLDAVSDRDFALEF 243 Query: 243 LSNASILMMHMSRMCEEVISWCSHEYQFVTLSDTFSTGSSIMPQKKNPDMAELIRGKSGR 302 L+ ASI MH+SRM EE++ W S +++FVTLSD FSTGSSIMPQKKNPD AELIR K GR Sbjct: 244 LAAASICAMHLSRMAEELVIWSSAQFRFVTLSDRFSTGSSIMPQKKNPDAAELIRAKIGR 303 Query: 303 VYANLFGLLTVMKALPLAYNKDLQEDKEGMFDTAETITVALDILAGMLSSMIVNDKHMAE 362 + LLTVMK LPLAY+KD+QEDKE +FD A+T+ +AL + GM+ M N + + Sbjct: 304 IVGANVALLTVMKGLPLAYSKDMQEDKEQVFDAADTLMLALAAMEGMVRDMTANRASLED 363 Query: 363 STQKDFSNATELADYLASK-GMPFRQAHEIVGKLILECSKNGHYLQDVPLERYQTISDLI 421 + FS AT+LAD+L + +PFR AH + G L+ G L D+ L Q++ I Sbjct: 364 AAASGFSTATDLADWLVRELNLPFRDAHHVTGTLVAMAEAKGCDLPDLSLAEMQSVHGAI 423 Query: 422 EEDVYETLKSHTAVERRHSLGGTGFEQVKWQIAEAKKAL 460 DV+E L H +V R S GGT QV+ Q+A K+ L Sbjct: 424 RADVFEVLGVHNSVASRTSYGGTAPSQVRAQVARWKERL 462 Lambda K H 0.318 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 558 Number of extensions: 20 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 460 Length of database: 463 Length adjustment: 33 Effective length of query: 427 Effective length of database: 430 Effective search space: 183610 Effective search space used: 183610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate 3609679 Dshi_3062 (argininosuccinate lyase (RefSeq))
to HMM TIGR00838 (argH: argininosuccinate lyase (EC 4.3.2.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00838.hmm # target sequence database: /tmp/gapView.28360.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00838 [M=455] Accession: TIGR00838 Description: argH: argininosuccinate lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.3e-184 599.6 0.1 2.6e-184 599.4 0.1 1.0 1 lcl|FitnessBrowser__Dino:3609679 Dshi_3062 argininosuccinate lyas Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Dino:3609679 Dshi_3062 argininosuccinate lyase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 599.4 0.1 2.6e-184 2.6e-184 1 454 [. 9 462 .. 9 463 .] 0.99 Alignments for each domain: == domain 1 score: 599.4 bits; conditional E-value: 2.6e-184 TIGR00838 1 lwggRlkkeldkavaefnaslsfDkelaeaDiegsiahtkaLakagilteeeakklieaLeelkeevkegklelevd 77 +wggR++ ++d +++ nas+ fD +la++Di+gs ah+++La++gil++++a+ ++e+L ++ +e++ g++ ++ lcl|FitnessBrowser__Dino:3609679 9 MWGGRFAAGPDAIMEAINASIGFDRRLARQDIDGSRAHAAMLAQQGILSSKDAEAIREGLLTVLSEIETGQFAFSAA 85 6**************************************************************************** PP TIGR00838 78 aeDiHlavErelidkvgedvgkklhtgrsRnDqvatdlrlylrdkvkelaealkdllkalvekAekevetlmpgytH 154 eDiH++vE++l + +ge +g +lhtgrsRnDqvatd++l++rd+++ l++ll+al+ +Ae+ + +mpg+tH lcl|FitnessBrowser__Dino:3609679 86 LEDIHMNVEARLKELIGEPAG-RLHTGRSRNDQVATDFKLWVRDQLDAADAGLLALLRALLAQAEAGADWVMPGFTH 161 *****************7766.******************************************************* PP TIGR00838 155 LqrAqPitlaHhllayaemlerDleRlldalkRvnksPlGsgAlagtsfeidrellaelLgFdavvensldavsdRD 231 Lq AqP+t++Hh++ay+em++rD R++da R+n++PlG++Alagtsf++dr+++a+ LgFd+ ++nsldavsdRD lcl|FitnessBrowser__Dino:3609679 162 LQTAQPVTWGHHMMAYVEMFARDRGRMQDARARMNECPLGAAALAGTSFPLDRDATAQALGFDRPAANSLDAVSDRD 238 ***************************************************************************** PP TIGR00838 232 fiiEllsaaallmvhlsrlaEelilfsseEfgfvelsdevssgssimPqKKnpDvaEliRgktgrviGnltglltil 308 f++E+l+aa+++++hlsr+aEel+++ss+ f fv+lsd++s+gssimPqKKnpD+aEliR+k gr++G ++llt++ lcl|FitnessBrowser__Dino:3609679 239 FALEFLAAASICAMHLSRMAEELVIWSSAQFRFVTLSDRFSTGSSIMPQKKNPDAAELIRAKIGRIVGANVALLTVM 315 ***************************************************************************** PP TIGR00838 309 KalPlaYnkDlqEdkealfdalktveellevvtgllkelkvnkerleeaakknfalatdlAdylvrk.GvPFReahe 384 K+lPlaY kD+qEdke++fda +t+ +l +++g++++++ n+ le+aa+++f++atdlAd+lvr+ +PFR+ah+ lcl|FitnessBrowser__Dino:3609679 316 KGLPLAYSKDMQEDKEQVFDAADTLMLALAAMEGMVRDMTANRASLEDAAASGFSTATDLADWLVRElNLPFRDAHH 392 ***************************************************************************** PP TIGR00838 385 ivGevvakaiekGkkleeltleelqklsekleedvlevldleeavekrdakGGtakeevekaieeakael 454 ++G +va a++kG +l +l+l+e+q ++ +++ dv+evl ++++v+ r+++GGta+++v+ +++ k++l lcl|FitnessBrowser__Dino:3609679 393 VTGTLVAMAEAKGCDLPDLSLAEMQSVHGAIRADVFEVLGVHNSVASRTSYGGTAPSQVRAQVARWKERL 462 *****************************************************************99987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (455 nodes) Target sequences: 1 (463 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.57 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory