Align IGP synthase, amidotransferase subunit (EC 4.3.2.10) (characterized)
to candidate 8501586 DvMF_2305 imidazole glycerol phosphate synthase subunit HisH (RefSeq)
Query= reanno::Miya:8501586 (212 letters) >FitnessBrowser__Miya:8501586 Length = 212 Score = 444 bits (1143), Expect = e-130 Identities = 212/212 (100%), Positives = 212/212 (100%) Query: 1 MLAILDYKAGNQTSVRRALDHLGIPCVITADPAVIAGAHGVIFPGVGAAGQAMNELLTTG 60 MLAILDYKAGNQTSVRRALDHLGIPCVITADPAVIAGAHGVIFPGVGAAGQAMNELLTTG Sbjct: 1 MLAILDYKAGNQTSVRRALDHLGIPCVITADPAVIAGAHGVIFPGVGAAGQAMNELLTTG 60 Query: 61 LDKVLKDQVQAGKPLLGICVGCQIMLDYSQENDTKALGIVPGECRLFNAAWTEEDGTPIR 120 LDKVLKDQVQAGKPLLGICVGCQIMLDYSQENDTKALGIVPGECRLFNAAWTEEDGTPIR Sbjct: 61 LDKVLKDQVQAGKPLLGICVGCQIMLDYSQENDTKALGIVPGECRLFNAAWTEEDGTPIR 120 Query: 121 VPHMGWNSIVQKRPCELLKGIEPEAEFYFVHSYYPAPPESYVIATCTYGEEFCAIHGGPG 180 VPHMGWNSIVQKRPCELLKGIEPEAEFYFVHSYYPAPPESYVIATCTYGEEFCAIHGGPG Sbjct: 121 VPHMGWNSIVQKRPCELLKGIEPEAEFYFVHSYYPAPPESYVIATCTYGEEFCAIHGGPG 180 Query: 181 LWAVQFHPEKSGRPGLALLRNFYAYCKEASRA 212 LWAVQFHPEKSGRPGLALLRNFYAYCKEASRA Sbjct: 181 LWAVQFHPEKSGRPGLALLRNFYAYCKEASRA 212 Lambda K H 0.321 0.139 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 332 Number of extensions: 13 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 212 Length of database: 212 Length adjustment: 21 Effective length of query: 191 Effective length of database: 191 Effective search space: 36481 Effective search space used: 36481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 45 (21.9 bits)
Align candidate 8501586 DvMF_2305 (imidazole glycerol phosphate synthase subunit HisH (RefSeq))
to HMM TIGR01855 (hisH: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (EC 2.4.2.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01855.hmm # target sequence database: /tmp/gapView.28943.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01855 [M=198] Accession: TIGR01855 Description: IMP_synth_hisH: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.3e-60 189.8 0.0 2.7e-60 189.6 0.0 1.0 1 lcl|FitnessBrowser__Miya:8501586 DvMF_2305 imidazole glycerol pho Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Miya:8501586 DvMF_2305 imidazole glycerol phosphate synthase subunit HisH (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 189.6 0.0 2.7e-60 2.7e-60 2 196 .. 3 203 .. 2 205 .. 0.96 Alignments for each domain: == domain 1 score: 189.6 bits; conditional E-value: 2.7e-60 TIGR01855 2 vvidygvgNlksvkkalervgaesevvkdskelekadklvlPGVGafkeamkklrelelellaekvvkkkkpvlgiC 78 +++dy++gN +sv++al+++g ++++d + a +++PGVGa+ +am++l ++l+++ +++v+++kp+lgiC lcl|FitnessBrowser__Miya:8501586 3 AILDYKAGNQTSVRRALDHLGIPCVITADPAVIAGAHGVIFPGVGAAGQAMNELLTTGLDKVLKDQVQAGKPLLGIC 79 79********************************************************99999************** PP TIGR01855 79 lGmQllfekseEgkevkglglikgkvkkleaek........kvPhiGWnevevvkesellkgleeearvYfvHsYav 147 +G Q++++ s+E +++k+lg+++g+ + ++a+ +vPh+GWn++ ++ +ellkg+e ea++YfvHsY+ lcl|FitnessBrowser__Miya:8501586 80 VGCQIMLDYSQE-NDTKALGIVPGECRLFNAAWteedgtpiRVPHMGWNSIVQKRPCELLKGIEPEAEFYFVHSYYP 155 ************.579*************987777889999***********************************9 PP TIGR01855 148 eleeeeavlakadygekfvaavekdnivgvQFHPEkSgktGlkllknfl 196 + e ++v+a+++yge+f a+ + +vQFHPEkSg+ Gl+ll+nf lcl|FitnessBrowser__Miya:8501586 156 APPE-SYVIATCTYGEEFCAIHGGPGLWAVQFHPEKSGRPGLALLRNFY 203 9987.8*****************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (198 nodes) Target sequences: 1 (212 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 7.38 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory