Align aspartate-tRNAAsn ligase (EC 6.1.1.23) (characterized)
to candidate CA265_RS19975 CA265_RS19975 aspartate--tRNA ligase
Query= BRENDA::Q9KDG1 (595 letters) >FitnessBrowser__Pedo557:CA265_RS19975 Length = 581 Score = 587 bits (1514), Expect = e-172 Identities = 300/583 (51%), Positives = 405/583 (69%), Gaps = 11/583 (1%) Query: 4 RTHHCGQLSEEQVNERVQLKGWVQRRRDLGQVIFVDLRDRSGVVQLVFNSDISQEALETA 63 RT CG L+ + E V L GWVQ+ RDLG + F+D+RDR G+ QLVFN D ++E ETA Sbjct: 3 RTVTCGALNLNNLGESVTLCGWVQKSRDLGGMTFIDIRDRYGITQLVFNMDDNRELCETA 62 Query: 64 EKVRNEYVLDVEGVVLKRDPSTVNDKIATGTIEVHVERLTILNKAKSLPFQIEANTDASE 123 + E+V+ G V++R S N K+ATG +E+ + L ILN AK PF I+ TD + Sbjct: 63 RTLGREFVIKAIGTVVER--SNKNPKMATGDVEIKISALEILNAAKLPPFMIDDETDGGD 120 Query: 124 DIRLKYRYLDLRRPDMQETMKLRHQTTKLIRDFLDGQEFFEIETPMLTKSTPEGARDYLV 183 ++R+KYRYLDLRR ++ + LRH+ + +R +LD +F E+ETP+L KSTPEGARD++V Sbjct: 121 ELRMKYRYLDLRRNPVRNNLVLRHKMAQSVRRYLDALDFIEVETPVLIKSTPEGARDFVV 180 Query: 184 PSRVHHGEFYALPQSPQIFKQLLMVSGFERYYQIVRCFRDEDLRADRQPEFTQIDIETSF 243 PSR++ GEFYALPQSPQ FKQLLMVSGF+RY+QIV+CFRDEDLRADRQPEFTQID E SF Sbjct: 181 PSRMNEGEFYALPQSPQTFKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF 240 Query: 244 MDKEDLLTMTENMMAKIMKEVKGLDVALPFPRMTYDDAMNRYGSDKPDTRFEMELIELSD 303 +++ED+L E ++ + KEV+ D+ PRM Y DAM YGSDKPDTRF M+ +EL+ Sbjct: 241 IEQEDILNTFEGLIRTLFKEVRNYDLP-EVPRMQYADAMRLYGSDKPDTRFAMQFVELNH 299 Query: 304 IVKDSDFKVFSSAIKSGGIVKGLNLKGGAGSLSRKEIDGLAEFVK--PYGAKGLAWLK-V 360 +VK F VF +A +V G+N K GA S +RK++D L +F+K GA GL + + Sbjct: 300 LVKGKGFPVFDNA----ELVVGINAK-GAASYTRKQLDELTDFIKRPQIGATGLIYARHN 354 Query: 361 EEGELKGPIAKFFAGETGAELQQAMGAEDGDLLFFAADKKEVVFDSLGALRLKLGKDFNL 420 E+G +K + KFF E + +A G E GDLL A + V L LRL++G L Sbjct: 355 EDGTIKSSVDKFFNEEDLKQWSEAFGTEKGDLLLILAGSTDKVRKQLNELRLEMGSRLGL 414 Query: 421 IDESKFNFLWVVDFPLVEYDEEAKRFVALHHPFTSPKQEDLTKLETDPASVRADAYDLVL 480 D++ F+ LWV+DFPL+E+DEE +R+ A+HHPFTSPK ED+ L+TDP +VRA+AYD+V+ Sbjct: 415 RDKNTFSALWVLDFPLLEWDEETERYHAMHHPFTSPKPEDIALLDTDPKNVRANAYDMVI 474 Query: 481 NGYELGGGSQRIYQRPVQEKMFAALGFTEEAAQKEFGFLLEAFEYGTPPHGGIALGLDRL 540 NG E+GGGS RI+ R +Q MF LGF+ E AQK+FGFL++AFE+G PPHGGIA G DRL Sbjct: 475 NGTEVGGGSIRIHDRALQALMFKHLGFSAEEAQKQFGFLMDAFEFGAPPHGGIAFGFDRL 534 Query: 541 VMLLAGRLNLRDTIAFPKTASASCLLTEAPGEVSLEQLLDLNL 583 + AG ++RD IAFPK S ++ ++P + +QL +L + Sbjct: 535 TSIFAGLDSIRDVIAFPKNNSGRDVMIDSPSTIDEKQLKELKI 577 Lambda K H 0.319 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 924 Number of extensions: 33 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 595 Length of database: 581 Length adjustment: 37 Effective length of query: 558 Effective length of database: 544 Effective search space: 303552 Effective search space used: 303552 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory