Alignments for a candidate for carA in Phaeobacter inhibens BS107

GapMind for Amino acid biosynthesis

Alignments for a candidate for carA in Phaeobacter inhibens BS107

Align Carbamoyl-phosphate synthase small chain; Carbamoyl-phosphate synthetase glutamine chain; EC 6.3.5.5 (characterized)
to candidate GFF653 PGA1_c06670 carbamoyl-phosphate synthase small chain

Query= SwissProt::P0A6F1
         (382 letters)



>FitnessBrowser__Phaeo:GFF653
          Length = 387

 Score =  380 bits (976), Expect = e-110
 Identities = 201/385 (52%), Positives = 260/385 (67%), Gaps = 14/385 (3%)

Query: 4   SALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNV 63
           +A L L DGT F+G   GA G  V E+ FNT+MTGYQEI+TDPSY+ QIVT T+PHIGN 
Sbjct: 10  TACLALADGTIFYGNGFGAYGETVAELCFNTAMTGYQEIMTDPSYAGQIVTFTFPHIGNT 69

Query: 64  GTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYLKRHNIVAIADIDTRKLTRLLR 123
           G    D+E++   A G+V++  P +ASN+R TE+L  +L R N +AI  IDTR+LTR +R
Sbjct: 70  GVTPEDDETADPVAAGMVVKWDPTLASNWRATEELKDWLTRTNRIAIGGIDTRRLTRAIR 129

Query: 124 EKGAQNGCIIAGDNPDAALALE----KARAFPGLNGMDLAKEVTTAEAYSWTQGSWTLTG 179
           + GA +  +    +PD    +E    KAR + GL G+DLAK+VT +++Y W +  W    
Sbjct: 130 QLGAPH--VALAHDPDGNFDIEALVAKAREWSGLEGLDLAKDVTCSQSYRWDEMRWAW-- 185

Query: 180 GLPEAKKEDELPFH-VVAYDFGAKRNILRMLVDRGCRLTIVPAQTSAEDVLKMNPDGIFL 238
             PE     E P H VVA D+GAKRNILR L   GC +T++PA  +AE+VL  +PDG+FL
Sbjct: 186 --PEGYTRQEAPKHKVVAIDYGAKRNILRCLASAGCDVTVLPASATAEEVLAHSPDGVFL 243

Query: 239 SNGPGDPAPC-DYAITAIQKFLET-DIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHP 296
           SNGPGDPA   +YA+  IQ+ L+T D+P+FGICLGHQ+LALA G KTVKM  GHHG NHP
Sbjct: 244 SNGPGDPAATGEYAVPMIQQILDTTDLPMFGICLGHQMLALALGGKTVKMNHGHHGANHP 303

Query: 297 VKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFDGTLQGIHRTDKPAFSFQGHPEA 356
           VK+     V IT+ NHGFAVD  +LP  +  TH SLFDG+  GI  +D+P +S Q HPEA
Sbjct: 304 VKENATGKVEITSMNHGFAVDGQSLPDGVEETHVSLFDGSNCGIRISDRPVYSVQHHPEA 363

Query: 357 SPGPHDAAPLFDHFIELIEQYRKTA 381
           SPGP D+  LF+ F E +   RK+A
Sbjct: 364 SPGPQDSFYLFERFAEAMAA-RKSA 387


Lambda     K      H
   0.318    0.135    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 477
Number of extensions: 22
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 387
Length adjustment: 30
Effective length of query: 352
Effective length of database: 357
Effective search space:   125664
Effective search space used:   125664
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate GFF653 PGA1_c06670 (carbamoyl-phosphate synthase small chain)
to HMM TIGR01368 (carA: carbamoyl-phosphate synthase, small subunit (EC 6.3.5.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01368.hmm
# target sequence database:        /tmp/gapView.30069.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01368  [M=361]
Accession:   TIGR01368
Description: CPSaseIIsmall: carbamoyl-phosphate synthase, small subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
   6.8e-135  435.6   0.0   7.8e-135  435.4   0.0    1.0  1  lcl|FitnessBrowser__Phaeo:GFF653  PGA1_c06670 carbamoyl-phosphate 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Phaeo:GFF653  PGA1_c06670 carbamoyl-phosphate synthase small chain
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  435.4   0.0  7.8e-135  7.8e-135       1     360 [.      11     382 ..      11     383 .. 0.95

  Alignments for each domain:
  == domain 1  score: 435.4 bits;  conditional E-value: 7.8e-135
                         TIGR01368   1 atlvledGtvfegksfgaekevvGevvFnTsmtGYqEiltDpsYkgqivvltyplignygvneedaeskkikvkglv 77 
                                       a l+l+dGt+f g +fga +e+v+e++FnT+mtGYqEi+tDpsY+gqiv++t p+ign+gv++ed e   + ++g+v
  lcl|FitnessBrowser__Phaeo:GFF653  11 ACLALADGTIFYGNGFGAYGETVAELCFNTAMTGYQEIMTDPSYAGQIVTFTFPHIGNTGVTPEDDETADPVAAGMV 87 
                                       5799************************************************************************* PP

                         TIGR01368  78 vkelskevsnyrakesLeeflkeegivaiegvDTRalvkklRekgsmkavistekse...keelvekakespkvkev 151
                                       vk   + +sn+ra+e+L+++l++++ +ai g+DTR l++ +R+ g+ + +++++       e lv+ka+e   ++++
  lcl|FitnessBrowser__Phaeo:GFF653  88 VKWDPTLASNWRATEELKDWLTRTNRIAIGGIDTRRLTRAIRQLGAPHVALAHDPDGnfdIEALVAKAREWSGLEGL 164
                                       *************************************************99998765555899999999******** PP

                         TIGR01368 152 nlvkevstkeayeleq......k..akkegkklrvvvidlGvKenilreLvkrgvevtvvpadtsaeeikklnpdgi 220
                                       +l+k+v++++ y++ +      +  +++e  k++vv+id+G K+nilr+L++ g++vtv+pa+++aee+ + +pdg+
  lcl|FitnessBrowser__Phaeo:GFF653 165 DLAKDVTCSQSYRWDEmrwawpEgyTRQEAPKHKVVAIDYGAKRNILRCLASAGCDVTVLPASATAEEVLAHSPDGV 241
                                       ***************976655516567777779******************************************** PP

                         TIGR01368 221 llsnGPGdPaav.eeaietvkkllea.kiPifGIclGhqllalalgaktyklkfGhrGaNhpvkdlktgrveitsqN 295
                                       +lsnGPGdPaa  e+a+ +++++l++ ++P+fGIclGhq+lalalg+kt+k++ Gh+GaNhpvk+ +tg+veits N
  lcl|FitnessBrowser__Phaeo:GFF653 242 FLSNGPGDPAATgEYAVPMIQQILDTtDLPMFGICLGHQMLALALGGKTVKMNHGHHGANHPVKENATGKVEITSMN 318
                                       *********776377899******9879************************************************* PP

                         TIGR01368 296 HgyavdeeslkeeelevthvnlnDgtveglehkelpvfsvQyHPeaspGphdteylFdefvelik 360
                                       Hg+avd +sl+ + +e thv+l+Dg++ g++ ++ pv+svQ+HPeaspGp+d+ ylF++f e+++
  lcl|FitnessBrowser__Phaeo:GFF653 319 HGFAVDGQSLPDG-VEETHVSLFDGSNCGIRISDRPVYSVQHHPEASPGPQDSFYLFERFAEAMA 382
                                       *********8855.***********************************************9986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (361 nodes)
Target sequences:                          1  (387 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 11.62
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis