Align 3-dehydroquinate dehydratase (EC 4.2.1.10) (characterized)
to candidate GFF585 PS417_02975 3-dehydroquinate dehydratase
Query= BRENDA::O30557 (147 letters) >FitnessBrowser__WCS417:GFF585 Length = 151 Score = 270 bits (691), Expect = 5e-78 Identities = 134/147 (91%), Positives = 141/147 (95%) Query: 1 MATLLVLHGPNLNLLGTREPGTYGSTTLGQINQDLERRAREAGHHLLHLQSNAEYELIDR 60 MATLLVLHGPNLNLLGTREPG YG+ TL QIN DLERRAREAGHHLL+LQSNAEYELIDR Sbjct: 1 MATLLVLHGPNLNLLGTREPGVYGAVTLDQINLDLERRAREAGHHLLYLQSNAEYELIDR 60 Query: 61 IHAARDEGVDFIIINPAAFTHTSVALRDALLAVSIPFIEVHLSNVHKREPFRHHSYFSDV 120 IHAAR EGVDFI+INPAAFTHTSVALRDALLAVSIPFIEVHLSNVHKRE FRHHSYFSDV Sbjct: 61 IHAARGEGVDFILINPAAFTHTSVALRDALLAVSIPFIEVHLSNVHKREAFRHHSYFSDV 120 Query: 121 AVGVICGLGATGYRLALESALEQLQRP 147 AVGVICGLGA+GYRLALE+ALEQL++P Sbjct: 121 AVGVICGLGASGYRLALEAALEQLEQP 147 Lambda K H 0.322 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 140 Number of extensions: 2 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 147 Length of database: 151 Length adjustment: 16 Effective length of query: 131 Effective length of database: 135 Effective search space: 17685 Effective search space used: 17685 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 42 (20.8 bits)
Align candidate GFF585 PS417_02975 (3-dehydroquinate dehydratase)
to HMM TIGR01088 (aroQ: 3-dehydroquinate dehydratase, type II (EC 4.2.1.10))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01088.hmm # target sequence database: /tmp/gapView.13472.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01088 [M=141] Accession: TIGR01088 Description: aroQ: 3-dehydroquinate dehydratase, type II Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5e-65 203.6 0.1 5.6e-65 203.4 0.1 1.0 1 lcl|FitnessBrowser__WCS417:GFF585 PS417_02975 3-dehydroquinate deh Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF585 PS417_02975 3-dehydroquinate dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 203.4 0.1 5.6e-65 5.6e-65 2 140 .. 4 143 .. 3 144 .. 0.98 Alignments for each domain: == domain 1 score: 203.4 bits; conditional E-value: 5.6e-65 TIGR01088 2 ilvlnGPnlnlLGkrepkvyGsltleeieelleeaakelevevevfqsnsegelidkihealeq.vdgivinpaal 76 +lvl+GPnlnlLG+rep+vyG++tl++i+ le+ a+e++ ++ ++qsn e+elid+ih a ++ vd+i+inpaa+ lcl|FitnessBrowser__WCS417:GFF585 4 LLVLHGPNLNLLGTREPGVYGAVTLDQINLDLERRAREAGHHLLYLQSNAEYELIDRIHAARGEgVDFILINPAAF 79 79************************************************************999*********** PP TIGR01088 77 thtsvalrDalaavslPvvevhlsnvhareefrkksvlaevakGvivGlGakgyklalealvea 140 thtsvalrDal+avs+P++evhlsnvh+re fr++s++++va Gvi+GlGa+gy+lalea++e+ lcl|FitnessBrowser__WCS417:GFF585 80 THTSVALRDALLAVSIPFIEVHLSNVHKREAFRHHSYFSDVAVGVICGLGASGYRLALEAALEQ 143 ***********************************************************99886 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (141 nodes) Target sequences: 1 (151 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 4.61 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory