Protein AZOBR_RS32335 in Azospirillum brasilense Sp245
Annotation: FitnessBrowser__azobra:AZOBR_RS32335
Length: 580 amino acids
Source: azobra in FitnessBrowser
Candidate for 12 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-fructose catabolism | fruA | hi | Fructose phosphotransferase system, IIB/IIC components (characterized, see rationale) | 100% | 100% | 1097 | The fructose porter, FruA (fructose-1-P forming IIABC) (Delobbe et al. 1975) FruA is 39% identical to 4.A.2.1.1). fructose can be metabolized to Fru-1-P via this system as well as Fru-6-P by another PTS system | 47% | 421.4 |
| sucrose catabolism | fruA | hi | Fructose phosphotransferase system, IIB/IIC components (characterized, see rationale) | 100% | 100% | 1097 | The fructose porter, FruA (fructose-1-P forming IIABC) (Delobbe et al. 1975) FruA is 39% identical to 4.A.2.1.1). fructose can be metabolized to Fru-1-P via this system as well as Fru-6-P by another PTS system | 47% | 421.4 |
| D-fructose catabolism | fruII-ABC | med | The fructose porter, FruA (fructose-1-P forming IIABC) (Delobbe et al. 1975) FruA is 39% identical to 4.A.2.1.1). fructose can be metabolized to Fru-1-P via this system as well as Fru-6-P by another PTS system (characterized) | 47% | 74% | 421.4 | PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 | 68% | 753.1 |
| sucrose catabolism | fruII-ABC | med | The fructose porter, FruA (fructose-1-P forming IIABC) (Delobbe et al. 1975) FruA is 39% identical to 4.A.2.1.1). fructose can be metabolized to Fru-1-P via this system as well as Fru-6-P by another PTS system (characterized) | 47% | 74% | 421.4 | PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 | 68% | 753.1 |
| D-mannose catabolism | manP | med | protein-Npi-phosphohistidine-D-mannose phosphotransferase (EC 2.7.1.191) (characterized) | 45% | 71% | 408.7 | PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 | 68% | 753.1 |
| D-fructose catabolism | fruII-C | med | Sugar phosphotransferase system IIC component, component of Fructose-specific Enzyme I-HPr-Enzyme IIABC complex, all encoded within a single operon with genes in the order: ptsC (IIC), ptsA (IIA), ptsH (HPr), ptsI (Enzyme I) and ptsB (IIB) (characterized) | 46% | 92% | 299.7 | PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 | 68% | 753.1 |
| sucrose catabolism | fruII-C | med | Sugar phosphotransferase system IIC component, component of Fructose-specific Enzyme I-HPr-Enzyme IIABC complex, all encoded within a single operon with genes in the order: ptsC (IIC), ptsA (IIA), ptsH (HPr), ptsI (Enzyme I) and ptsB (IIB) (characterized) | 46% | 92% | 299.7 | PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 | 68% | 753.1 |
| D-fructose catabolism | fruII-B | med | PTS system, fructose-specific, IIB subunnit, component of Fructose Enzyme II complex (IIAFru - IIBFru - IICFru) (based on homology) (characterized) | 46% | 99% | 109.4 | PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 | 68% | 753.1 |
| sucrose catabolism | fruII-B | med | PTS system, fructose-specific, IIB subunnit, component of Fructose Enzyme II complex (IIAFru - IIBFru - IICFru) (based on homology) (characterized) | 46% | 99% | 109.4 | PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 | 68% | 753.1 |
| D-ribose catabolism | fru2-IIB | med | PTS system, fructose-specific, IIB component, component of D-allose/D-ribose transporting Enzyme II complex (Fru2; IIA/IIB/IIC) (Patron et al. 2017). This system is similar to Frz of E. coli (TC#4.A.2.1.9) which is involved in environmental sensing, host adaptation and virulence (characterized) | 42% | 98% | 85.9 | PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 | 68% | 753.1 |
| xylitol catabolism | fruI | lo | The fructose inducible fructose/xylitol porter, FruI (characterized) | 38% | 83% | 332.4 | PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 | 68% | 753.1 |
| D-ribose catabolism | fru2-IIC | lo | PTS system, fructose-specific, IIC component, component of D-allose/D-ribose transporting Enzyme II complex (Fru2; IIA/IIB/IIC) (Patron et al. 2017). This system is similar to Frz of E. coli (TC#4.A.2.1.9) which is involved in environmental sensing, host adaptation and virulence (characterized) | 36% | 93% | 209.1 | PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 | 68% | 753.1 |
Sequence Analysis Tools
View AZOBR_RS32335 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
MANMLAVIAAGDLSTQAVLAAEALRKAAAALGHTIQVEVRSSLGVRNTLPTGAAQGAQGV
ILVGSGDLGEERFAGLKRSAAALDAVLRDARAVLEQALATAPAQAPAQTGTKKIVAITSC
PTGIAHTFMAAEGIQQAAQALGHAVRVETQGSVGARDTLTEQEIREADVVLIAADTQVDL
ARFAGKRVFKSGTKPAINDGRALVERALAEAQPHGTAPALADGVAAGKAAKAEHAAAQRS
GPYKHLMTGVSFMLPFVVTGGLLIAIAFALGGIYVFEDSQQGTLGNALFQIGAKGAFALM
VPALAGYIAFSIADRPGITPGMVGGILAANLGAGFLGGIVAGFIAGYATSFLNRNIRLHK
NLEGLKPVLILPLLGSLVTGLAMIYVVGAPVAEALATLSAWLKGMQGSSAILLGLLIGAM
MAFDMGGPVNKAAYAFSTGLIASQVYTPMAAAMAAGMVPPLGLALATKLFADRFTREERE
AGNAAGILGIAFITEGAIPFAARDPLRVIPALVLGAALTGAISMGIGAELKVPHGGIFVL
PIPNAVTHLAGYVVALVAGTVTTAVALRFLKRPVSSVVTA
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory