Alignments for a candidate for fadA in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for fadA in Pseudomonas fluorescens FW300-N1B4

Align acetyl-CoA C-acetyltransferase (EC 2.3.1.16) (characterized)
to candidate Pf1N1B4_5835 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9)

Query= metacyc::MONOMER-20831
         (393 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5835
          Length = 393

 Score =  674 bits (1739), Expect = 0.0
 Identities = 337/393 (85%), Positives = 371/393 (94%)

Query: 1   MQDVVIVAATRTAVGSFQGSLASIPAPELGAAVIRRLLEQTGLDPAQVDEVILGQVLTAG 60
           MQ+VVIVAATRTA+GSFQGSLA++ A +LGAAVIR+LL QTGLDPAQVDEVI+GQVLTAG
Sbjct: 1   MQEVVIVAATRTAIGSFQGSLANVSAVDLGAAVIRQLLAQTGLDPAQVDEVIMGQVLTAG 60

Query: 61  SGQNPARQASILAGLPHAVPSLTLNKVCGSGLKALHLGAQAIRCGDAEVIIAGGMENMSL 120
           +GQNPARQA+I AGLP AVP++TLNKVCGSGLKALHL AQAIRCGDAEVIIAGG ENMSL
Sbjct: 61  AGQNPARQAAIKAGLPFAVPAMTLNKVCGSGLKALHLAAQAIRCGDAEVIIAGGQENMSL 120

Query: 121 APYVLPAARTGLRMGHAKMIDSMITDGLWDAFNDYHMGITAENLVDKYGISREAQDAFAA 180
           + YV+P ARTGLRMGHA+++D+MI+DGLWDAFNDYHMGITAENL +KY ++RE QDAFAA
Sbjct: 121 SNYVMPGARTGLRMGHAQIVDTMISDGLWDAFNDYHMGITAENLAEKYSLTREQQDAFAA 180

Query: 181 ASQQKATAAIEAGRFVDEITPILIPQRKGDPVAFAVDEQPRAGTTAESLAKLKPAFKKDG 240
           ASQQKA AAIEAGRF DEITPILIPQRKGDP++FA DEQPRAGTTA+SL KLK AFKKDG
Sbjct: 181 ASQQKAVAAIEAGRFADEITPILIPQRKGDPLSFATDEQPRAGTTADSLGKLKAAFKKDG 240

Query: 241 SVTAGNASSLNDGAAAVLLMSADKAKALGLPVLARIASYANAGVDPAIMGIGPVSATRRC 300
           SVTAGNASSLNDGAAAV+LMSA+KAKALGLPVLA+IA+YANAGVDPAIMGIGPVSATRRC
Sbjct: 241 SVTAGNASSLNDGAAAVILMSAEKAKALGLPVLAKIAAYANAGVDPAIMGIGPVSATRRC 300

Query: 301 LDKAGWSLGDLDLIEANEAFAAQSLAVGKELEWDAEKVNVNGGAIAIGHPIGASGCRVLV 360
           LDK GWS+G LDLIEANEAFAAQSLAV K+LEWD  KVNVNGGAIA+GHPIGASGCRVLV
Sbjct: 301 LDKTGWSIGQLDLIEANEAFAAQSLAVAKDLEWDLNKVNVNGGAIALGHPIGASGCRVLV 360

Query: 361 TLLHEMIKRDAKKGLATLCIGGGQGVALALERS 393
           TLLHEMIK+DAKKGLATLCIGGGQGVALA+ER+
Sbjct: 361 TLLHEMIKQDAKKGLATLCIGGGQGVALAIERA 393


Lambda     K      H
   0.317    0.133    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 559
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 393
Length of database: 393
Length adjustment: 31
Effective length of query: 362
Effective length of database: 362
Effective search space:   131044
Effective search space used:   131044
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate Pf1N1B4_5835 (3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9))
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01930.hmm
# target sequence database:        /tmp/gapView.31592.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01930  [M=385]
Accession:   TIGR01930
Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                      Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                      -----------
     2e-151  490.3  11.3   2.2e-151  490.1  11.3    1.0  1  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5835  3-ketoacyl-CoA thiolase (EC 2.3.


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5835  3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  490.1  11.3  2.2e-151  2.2e-151       1     385 []       6     391 ..       6     391 .. 0.99

  Alignments for each domain:
  == domain 1  score: 490.1 bits;  conditional E-value: 2.2e-151
                                      TIGR01930   1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaRea 64 
                                                    iv+a+Rt+ig+++gsl+++sa+dL+aavi++ll+++gldp+++devi+G+vl+ag+++n+aR+a
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5835   6 IVAATRTAIGSFQGSLANVSAVDLGAAVIRQLLAQTGLDPAQVDEVIMGQVLTAGAGQNPARQA 69 
                                                    8*************************************************************** PP

                                      TIGR01930  65 alaaglpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrres 128
                                                    a++aglp  vpa+t+n+vC+Sgl+A++laaq+i++G+a+v++aGG E+mS + ++++ +  r +
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5835  70 AIKAGLPFAVPAMTLNKVCGSGLKALHLAAQAIRCGDAEVIIAGGQENMSLSNYVMPGA--RTG 131
                                                    *******************************************************9997..89* PP

                                      TIGR01930 129 lklgkakledqllkdl...vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkf 189
                                                    l++g+a+  d++++d+   + ++++mg+tAenla+ky+++Re+qD++a++S+qka +Aie+g+f
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5835 132 LRMGHAQIVDTMISDGlwdAFNDYHMGITAENLAEKYSLTREQQDAFAAASQQKAVAAIEAGRF 195
                                                    *******************99******************************************* PP

                                      TIGR01930 190 kdeivpvevkgk...kkvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaal 250
                                                    +dei+p+ ++++   + ++++De++r++tt+++L+kLk+afk+ +gs vtAgN+s+lnDGAaa+
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5835 196 ADEITPILIPQRkgdPLSFATDEQPRAGTTADSLGKLKAAFKK-DGS-VTAGNASSLNDGAAAV 257
                                                    *********99999999************************95.9*7.**************** PP

                                      TIGR01930 251 llmseevakelgltplarivsaavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFA 314
                                                    +lms e+ak+lgl +la+i ++a+agvdp++mg+gpv A++++L+k+g+si ++dl+E nEAFA
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5835 258 ILMSAEKAKALGLPVLAKIAAYANAGVDPAIMGIGPVSATRRCLDKTGWSIGQLDLIEANEAFA 321
                                                    **************************************************************** PP

                                      TIGR01930 315 aqvlavekelgsldlekvNvnGGAiAlGHPlGasGarivltllkeLkergkkyGlatlCvggGq 378
                                                    aq lav+k+l+  dl+kvNvnGGAiAlGHP+GasG+r+++tll+e+ ++++k GlatlC+ggGq
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5835 322 AQSLAVAKDLE-WDLNKVNVNGGAIALGHPIGASGCRVLVTLLHEMIKQDAKKGLATLCIGGGQ 384
                                                    ***********.88************************************************** PP

                                      TIGR01930 379 GaAvile 385
                                                    G+A+ +e
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5835 385 GVALAIE 391
                                                    ***9886 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (385 nodes)
Target sequences:                          1  (393 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 9.19
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources