Clusters of Characterized Proteins

Clustering the characterized proteins for PRAI (phosphoribosylanthranilate isomerase) in L-tryptophan biosynthesis

Or see other characterized proteins similar to PRAI

Or see all steps for L-tryptophan biosynthesis

Or cluster curated proteins matching a keyword

Definition of PRAI

• Curated proteins or TIGRFams with EC 5.3.1.24
• UniProt sequence Q8AAD7_BACTN: RecName: Full=N-(5'-phosphoribosyl)anthranilate isomerase {ECO:0000256|HAMAP-Rule:MF_00135}; Short=PRAI {ECO:0000256|HAMAP-Rule:MF_00135}; EC=5.3.1.24 {ECO:0000256|HAMAP-Rule:MF_00135};
• UniProt sequence A0A0H3CC53_CAUVN: RecName: Full=N-(5'-phosphoribosyl)anthranilate isomerase {ECO:0000256|HAMAP-Rule:MF_00135}; Short=PRAI {ECO:0000256|HAMAP-Rule:MF_00135}; EC=5.3.1.24 {ECO:0000256|HAMAP-Rule:MF_00135};
• UniProt sequence TRPF_RHIME: RecName: Full=N-(5'-phosphoribosyl)anthranilate isomerase {ECO:0000255|HAMAP-Rule:MF_00135}; Short=PRAI {ECO:0000255|HAMAP-Rule:MF_00135}; EC=5.3.1.24 {ECO:0000255|HAMAP-Rule:MF_00135};
• UniProt sequence A0A1L6JC25_9SPHN: RecName: Full=N-(5'-phosphoribosyl)anthranilate isomerase {ECO:0000256|HAMAP-Rule:MF_00135}; Short=PRAI {ECO:0000256|HAMAP-Rule:MF_00135}; EC=5.3.1.24 {ECO:0000256|HAMAP-Rule:MF_00135};
• Ignore hits to items matching EC 5.3.1.16 when looking for 'other' hits
• Comment: PRAI is sometimes known as trpF or as part of trpC (if fused to IGPS). In Bacteroides thetaiotaomicron, BT0528 (Q8AAD7_BACTN) has a PRAI domain (PF00697), is auxotrophic, and clusters with trp synthesis genes Similarly, annotate CCNA_03659 (A0A0H3CC53_CAUVN), SMc02767 (TRPF_RHIME), and Ga0059261_0237 (A0A1L6JC25_9SPHN). Some bacteria have a bifunctional phosphoribosylanthranilate isomerase (EC 5.3.1.24) and isomerase in histidine biosynthesis (hisA, EC 5.3.1.16), for instance P16250. (In both reactions a N-modified-1-amino-ribofuranose-5-phosphate rearranges to a linear N-modified-1-amino-ribulose-5-phosphate.) So hits to hisA are ignored.

Fetched 15 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 13 of these sequences

Found 3 clusters of similar sequences. Another 3 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 208-275 amino acids (not heteromeric)

CH_008324 phosphoribosylanthranilate isomerase; EC 5.3.1.24
PFams: PRAI
213 amino acids: PaperBLAST, CDD, Compare to cluster

Q8U092 phosphoribosylanthranilate isomerase (EC 5.3.1.24)
PFams: PRAI
208 amino acids: PaperBLAST, CDD, Compare to cluster

CH_021917 phosphoribosylanthranilate isomerase; EC 5.3.1.24
PFams: PRAI
235 amino acids: PaperBLAST, CDD, Compare to cluster

Q42440 N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic; EC 5.3.1.24
PFams: PRAI
275 amino acids: PaperBLAST, CDD, Compare to cluster

Q42527 N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic; EC 5.3.1.24
PFams: PRAI
275 amino acids: PaperBLAST, CDD, Compare to cluster

A0A0H3CC53_CAUVN N-(5'-phosphoribosyl)anthranilate isomerase {ECO:0000256|HAMAP-Rule:MF_00135}; Short=PRAI {ECO:0000256|HAMAP-Rule:MF_00135}; EC=5.3.1.24 {ECO:0000256|HAMAP-Rule:MF_00135}
219 amino acids: PaperBLAST, CDD, Compare to cluster

A0A1L6JC25_9SPHN N-(5'-phosphoribosyl)anthranilate isomerase {ECO:0000256|HAMAP-Rule:MF_00135}; Short=PRAI {ECO:0000256|HAMAP-Rule:MF_00135}; EC=5.3.1.24 {ECO:0000256|HAMAP-Rule:MF_00135}
212 amino acids: PaperBLAST, CDD, Compare to cluster

TRPF_RHIME N-(5'-phosphoribosyl)anthranilate isomerase {ECO:0000255|HAMAP-Rule:MF_00135}; Short=PRAI {ECO:0000255|HAMAP-Rule:MF_00135}; EC=5.3.1.24 {ECO:0000255|HAMAP-Rule:MF_00135}
215 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 224-226 amino acids (not heteromeric)

P00912 N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; EC 5.3.1.24
PFams: PRAI
224 amino acids: PaperBLAST, CDD, Compare to cluster

CH_123631 N-(5'-phosphoribosyl)anthranilate isomerase; EC 5.3.1.24
PFams: PRAI
226 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 3 453-531 amino acids (not heteromeric)

PRAI-IGPS fused indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (EC 4.1.1.48; EC 5.3.1.24)
P00909 phosphoribosylanthranilate isomerase (EC 5.3.1.24)
PRAI-IGPS indole-3-glycerol phosphate synthase (EC 4.1.1.48; EC 5.3.1.24)
PFams: IGPS, PRAI
453 amino acids: PaperBLAST, CDD, Compare to cluster

P24920 phosphoribosylanthranilate isomerase (EC 5.3.1.24)
PFams: IGPS, PRAI
531 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/3 heteromeric)

B0B7P4 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one isomerase/dehydratase (EC 4.2.1.160); phosphoribosylanthranilate isomerase (EC 5.3.1.24)
MONOMER-18791 phosphoribosylanthranilate isomerase (EC 5.3.1.24; EC 4.2.1.160)
PFams: PRAI
208 amino acids: PaperBLAST, CDD

P16250 Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16; EC 5.3.1.24
P16250 phosphoribosylanthranilate isomerase (EC 5.3.1.24)
PFams: His_biosynth
240 amino acids: PaperBLAST, CDD

Q8AAD7_BACTN N-(5'-phosphoribosyl)anthranilate isomerase {ECO:0000256|HAMAP-Rule:MF_00135}; Short=PRAI {ECO:0000256|HAMAP-Rule:MF_00135}; EC=5.3.1.24 {ECO:0000256|HAMAP-Rule:MF_00135}
207 amino acids: PaperBLAST, CDD
(similar to CharProtDB::CH_021917 from Cluster 1, but similarity to seed sequence is below thresholds)