Align Succinyl-diaminopimelate desuccinylase; SDAP desuccinylase; EC 3.5.1.18; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase (uncharacterized)
to candidate GFF3695 PS417_18910 acetylornithine deacetylase
Query= curated2:B2FIC0 (375 letters) >FitnessBrowser__WCS417:GFF3695 Length = 385 Score = 88.2 bits (217), Expect = 3e-22 Identities = 73/238 (30%), Positives = 108/238 (45%), Gaps = 20/238 (8%) Query: 49 NLWATHGLG-APVLVLLGHTDVVPTGPRESWTSDPFTPHIRDGVLYGRGAADMKGSVAAF 107 NL AT G P +VL GHTDVVP + W+ PF +DG LYGRG ADMKG +A Sbjct: 51 NLLATIGPAEVPGIVLSGHTDVVPV-EGQRWSVAPFDLTEKDGKLYGRGTADMKGYIACV 109 Query: 108 VVAAEQFVADHPDHPGTLAVLLTSDEEGDAIDGVRHVARLFAARGQRIDWCITGEPSSTA 167 + V P + + L+ DEE + GVR + F + + C+ GEP+ Sbjct: 110 LACVPALVKASLRMP--VHIALSYDEEVGCL-GVRSLIERFHEQPVKPLLCVIGEPTELK 166 Query: 168 TLGDLLRVGRRGSLSAKLRVQGVQGHVAYPEKARNPIHQAAPALAEL--------CARRW 219 + +G +G L+ + V G H AY N I AA ++EL + Sbjct: 167 PV-----LGHKGKLAMRCHVHGAACHSAYAPAGVNAIEYAACLISELVRLGETLKAPQHL 221 Query: 220 DDGYESFPPTSLQISNIHAGTGANNVIPGELDVDFNIRYNPHWDAPKLEAEITALLER 277 D + P S + + G A N++P + DF +R P D ++ ++ A E+ Sbjct: 222 DQRFA--PAFSTVQTGLINGGKALNIVPQDCSFDFEVRSLPAQDPWQVARQLQAYAEQ 277 Lambda K H 0.319 0.137 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 321 Number of extensions: 18 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 375 Length of database: 385 Length adjustment: 30 Effective length of query: 345 Effective length of database: 355 Effective search space: 122475 Effective search space used: 122475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory