Align candidate NP_347216.1 CA_C0578 (B12-dependent methionine synthase)
to HMM PF02965 (Met_synt_B12)
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/PF02965.21.hmm # target sequence database: /tmp/gapView.12694.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Met_synt_B12 [M=273] Accession: PF02965.21 Description: Vitamin B12 dependent methionine synthase, activation domain Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.9e-116 373.3 0.0 8.4e-116 372.2 0.0 1.6 1 lcl|NCBI__GCF_000008765.1:NP_347216.1 CA_C0578 B12-dependent methionin Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000008765.1:NP_347216.1 CA_C0578 B12-dependent methionine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 372.2 0.0 8.4e-116 8.4e-116 2 272 .. 932 1197 .. 931 1198 .. 0.98 Alignments for each domain: == domain 1 score: 372.2 bits; conditional E-value: 8.4e-116 Met_synt_B12 2 leelveyidWtpffqaWelkgkypkiledekvgeeakklfkdAqamLkkiieekllkakavvglfpAnse 71 ++el++yidWt+ff++W++ ++ypki++d+k+g+ea+klfkdA++mL+ +++e+l+++++v+g+fpAns+ lcl|NCBI__GCF_000008765.1:NP_347216.1 932 INELRKYIDWTFFFTSWDMGMNYPKIMKDPKYGAEAQKLFKDANEMLDLLQKENLITCNGVFGIFPANSV 1001 89******************************************************************** PP Met_synt_B12 72 gddievyadesrseelatlhtLrqqaekeegkpnlclaDfvapkesgvkDyiGlFavtaglgieelakef 141 +ddie+y+d+ ++t++tLrqq+ +++++ +l+D++apk g+kDyiG+F+vtag+g++e+ +++ lcl|NCBI__GCF_000008765.1:NP_347216.1 1002 NDDIEIYTDKG----TVTINTLRQQQIL-KDSDYKALSDYIAPKGIGIKDYIGGFIVTAGIGAKEYSDKL 1066 *******7776....689*******765.7889************************************* PP Met_synt_B12 142 eaekddYsailvkaladrLaeAfaellhekvrkelWgyakdeklsneelikekYqgiRpApGYpacpdht 211 +++ ddY a ++k ++drLaeAf+ellh +vrke+Wgy++de+ls e+l+k +Y+gi+pA+GYp++pdh+ lcl|NCBI__GCF_000008765.1:NP_347216.1 1067 KKKCDDYGATMLKLICDRLAEAFSELLHLRVRKEYWGYSQDENLSLEKLLKGSYRGIKPAIGYPSIPDHS 1136 ********************************************************************** PP Met_synt_B12 212 ekktlfelldaeekigieLteslamtPaasvsGlyfahpearyFavgkiekdqvedyakrk 272 ek++lf+ll +++ig+eLtes++m P++sv+Glyfa+++a+yF+++ki+kdq+edya r lcl|NCBI__GCF_000008765.1:NP_347216.1 1137 EKAKLFDLLLGKTSIGVELTESYMMNPTSSVCGLYFANERAKYFNINKIGKDQLEDYAVRS 1197 **********************************************************996 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (273 nodes) Target sequences: 1 (1212 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 34.86 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory