Align Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.- (characterized)
to candidate NP_663089.1 CT2215 aspartyl/glutamyl-tRNA amidotransferase subunit B
Query= SwissProt::Q9RF06 (475 letters) >NCBI__GCF_000006985.1:NP_663089.1 Length = 475 Score = 462 bits (1188), Expect = e-134 Identities = 226/474 (47%), Positives = 325/474 (68%), Gaps = 1/474 (0%) Query: 1 MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAM 60 M++E V+GLEVH +L T+SK F A FG N+N + LA PG LPV+N R V+ A+ Sbjct: 1 MNYEIVVGLEVHCQLNTESKAFCGCSAKFGKPANTNVCPVCLALPGALPVLNARVVEDAV 60 Query: 61 RAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEVDGETKRIGITR 120 + +A N IA S RKNYFYPD PK YQISQ+++PI G I I+++ K + + R Sbjct: 61 KLGLATNCTIARHSILARKNYFYPDLPKGYQISQYEEPICSEGVIHIDLEEGGKDVRLVR 120 Query: 121 LHMEEDAGKSTHK-GEYSLVDLNRQGTPLIEIVSEPDIRSPKEAYAYLEKLRSIIQYTGV 179 +H+EEDAGKS H G+ + +D+NR G PL+EIVS PD+R+PKEA AYL+KLR I++Y G+ Sbjct: 121 IHIEEDAGKSIHDIGDDTYIDVNRCGVPLLEIVSYPDMRTPKEASAYLQKLRQIVRYLGI 180 Query: 180 SDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLSGG 239 SD MEEGSLRCDAN+S+RP G ++GT+ E+KN+NSF V + +EYE KR E + GG Sbjct: 181 SDGNMEEGSLRCDANVSVRPVGATEYGTRTEIKNMNSFRNVERAIEYEAKRHIEVIEGGG 240 Query: 240 EIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKERVRQTIPELPDER 299 I QETR +D +T MR KE + DYRYFP+PD+VP+ +DD R+++ +PE P++R Sbjct: 241 TIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDDGMIRRMQEELPEFPEDR 300 Query: 300 KAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQVEL 359 A++V+E G+PAYDA V+T+ +E++D+FEST++ D K +SNW+MG V L + +++ Sbjct: 301 AARFVSEFGIPAYDAGVITVDRELADYFESTVKVSGDAKASSNWVMGEVMRTLKEKYLDI 360 Query: 360 LDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGLVQISDEATLL 419 ++PE L G+IKLI G +S+ IAK+VF + A+ I+E GL Q+SD + Sbjct: 361 HKFAISPERLGGLIKLINAGAISNTIAKQVFEIMQQDEATAEAIVEREGLAQVSDRGAIE 420 Query: 420 KFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQELD 473 + E L+ N++ +E Y++GK + GF VGQ M+ KG+ANP++VN +L+ LD Sbjct: 421 AAIREILEANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKMVNDILRSMLD 474 Lambda K H 0.313 0.133 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 630 Number of extensions: 28 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 475 Length of database: 475 Length adjustment: 33 Effective length of query: 442 Effective length of database: 442 Effective search space: 195364 Effective search space used: 195364 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 51 (24.3 bits)
Align candidate NP_663089.1 CT2215 (aspartyl/glutamyl-tRNA amidotransferase subunit B)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00133.hmm # target sequence database: /tmp/gapView.26626.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00133 [M=481] Accession: TIGR00133 Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.3e-184 597.5 0.0 9.3e-184 597.3 0.0 1.0 1 lcl|NCBI__GCF_000006985.1:NP_663089.1 CT2215 aspartyl/glutamyl-tRNA am Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000006985.1:NP_663089.1 CT2215 aspartyl/glutamyl-tRNA amidotransferase subunit B # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 597.3 0.0 9.3e-184 9.3e-184 2 480 .. 1 473 [. 1 474 [. 0.99 Alignments for each domain: == domain 1 score: 597.3 bits; conditional E-value: 9.3e-184 TIGR00133 2 ieyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalnskiv 73 ++ye+v+GlEvH+qlnt+sK Fc cs ++ + + Ntnvcpvcl+lPGalPvlN ++v+ A+kl+la+n+ i lcl|NCBI__GCF_000006985.1:NP_663089.1 1 MNYEIVVGLEVHCQLNTESKAFCGCSAKFGK-PANTNVCPVCLALPGALPVLNARVVEDAVKLGLATNCTIA 71 69****************************9.99************************************65 PP TIGR00133 74 sevsvFdRKhYfYpDlPkgyqitqqdlPiaedGkleieleekekeigierlhlEeDtgksqykesdkdkqsl 145 s+ +RK+YfYpDlPkgyqi+q++ Pi+++G+++i+lee+ k +++ r+h+EeD+gks + d ++ lcl|NCBI__GCF_000006985.1:NP_663089.1 72 -RHSILARKNYFYPDLPKGYQISQYEEPICSEGVIHIDLEEGGKDVRLVRIHIEEDAGKSIHDIGD---DTY 139 .69***********************************************************9776...69* PP TIGR00133 146 vDfNRsgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlkGqekegtrvE 217 +D NR+gvPLlEiV+ Pd++++kea a+l+klrqi+ryl+isdg++eeGs+R+D+Nvs+r++G ++ gtr E lcl|NCBI__GCF_000006985.1:NP_663089.1 140 IDVNRCGVPLLEIVSYPDMRTPKEASAYLQKLRQIVRYLGISDGNMEEGSLRCDANVSVRPVGATEYGTRTE 211 ************************************************************************ PP TIGR00133 218 iKNlnslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRYfpePdlppieide 289 iKN+ns++++e+aieyE +R +++++ g +++qetr +d k t s+R Ke+++DYRYfp+Pdl+p+ +d+ lcl|NCBI__GCF_000006985.1:NP_663089.1 212 IKNMNSFRNVERAIEYEAKRHIEVIEGGGTIVQETRLWDADKLETRSMRGKEHAHDYRYFPDPDLVPVLVDD 283 ************************************************************************ PP TIGR00133 290 evvkekveeklpelPeakrirlkkeyglseedakvlvsdlelldafeevvklikepklavnWileellgeLn 361 ++++ ++e+lpe+Pe + +r+++e+g+ ++da v++ d+el+d+fe+ vk+++++k++ nW++ e++++L+ lcl|NCBI__GCF_000006985.1:NP_663089.1 284 GMIRR-MQEELPEFPEDRAARFVSEFGIPAYDAGVITVDRELADYFESTVKVSGDAKASSNWVMGEVMRTLK 354 ****9.****************************************************************** PP TIGR00133 362 kkkislaeallkpeelaeliklikegkisqksakelleellenkkdpkklieklgliqisdekelvkiveev 433 +k +++++++++pe l likli++g is+++ak+++e + +++ +++++e++gl q+sd+ ++ ++++e+ lcl|NCBI__GCF_000006985.1:NP_663089.1 355 EKYLDIHKFAISPERLGGLIKLINAGAISNTIAKQVFEIMQQDEATAEAIVEREGLAQVSDRGAIEAAIREI 426 ************************************************************************ PP TIGR00133 434 ikenpkevekyksgkekalkflvGqvmkktkgradpkevekllkell 480 ++ n+k++e+y+sgk ++++f+vGq m+k kg+a+pk v+ +l+ +l lcl|NCBI__GCF_000006985.1:NP_663089.1 427 LEANQKQLEQYRSGKTQLFGFFVGQCMQKMKGKANPKMVNDILRSML 473 ********************************************998 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (481 nodes) Target sequences: 1 (475 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.02s 00:00:00.04 Elapsed: 00:00:00.04 # Mc/sec: 5.09 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory