Align histidinol-phosphate aminotransferase (EC 2.6.1.9) (characterized)
to candidate Pf6N2E2_3251 Histidinol-phosphate aminotransferase (EC 2.6.1.9)
Query= reanno::pseudo6_N2E2:Pf6N2E2_3251 (350 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3251 Length = 350 Score = 704 bits (1818), Expect = 0.0 Identities = 350/350 (100%), Positives = 350/350 (100%) Query: 1 MSKFWSPFVKNLVPYVPGEQPKLTRLVKLNTNENPYGPSPKALAAMQTELNDNLRLYPDP 60 MSKFWSPFVKNLVPYVPGEQPKLTRLVKLNTNENPYGPSPKALAAMQTELNDNLRLYPDP Sbjct: 1 MSKFWSPFVKNLVPYVPGEQPKLTRLVKLNTNENPYGPSPKALAAMQTELNDNLRLYPDP 60 Query: 61 NSDLLKGAVARYYGVQSNQVFLGNGSDEVLAHIFHGLLQHDQPLLFPDISYSFYPVYCGL 120 NSDLLKGAVARYYGVQSNQVFLGNGSDEVLAHIFHGLLQHDQPLLFPDISYSFYPVYCGL Sbjct: 61 NSDLLKGAVARYYGVQSNQVFLGNGSDEVLAHIFHGLLQHDQPLLFPDISYSFYPVYCGL 120 Query: 121 YGIQFDAVPLDAQFQIDPADYAKPNGGIIFPNPNAPTGCLLALEAVEQILKANPDSVVVV 180 YGIQFDAVPLDAQFQIDPADYAKPNGGIIFPNPNAPTGCLLALEAVEQILKANPDSVVVV Sbjct: 121 YGIQFDAVPLDAQFQIDPADYAKPNGGIIFPNPNAPTGCLLALEAVEQILKANPDSVVVV 180 Query: 181 DEAYIDFGGETAISLVDRYPNLLVTQTLSKSRSLAGLRVGLAVGHPELIEALERIKNSFN 240 DEAYIDFGGETAISLVDRYPNLLVTQTLSKSRSLAGLRVGLAVGHPELIEALERIKNSFN Sbjct: 181 DEAYIDFGGETAISLVDRYPNLLVTQTLSKSRSLAGLRVGLAVGHPELIEALERIKNSFN 240 Query: 241 SYPLDRLANVGGAAAFDDREYFDRTCRLVIEHREWVVAQLQAKGFEVLPSAANFIFARHP 300 SYPLDRLANVGGAAAFDDREYFDRTCRLVIEHREWVVAQLQAKGFEVLPSAANFIFARHP Sbjct: 241 SYPLDRLANVGGAAAFDDREYFDRTCRLVIEHREWVVAQLQAKGFEVLPSAANFIFARHP 300 Query: 301 RHDAAGLAAKLREQGVIVRHFKQERIAQFLRISIGTPEQNQALIEALGEL 350 RHDAAGLAAKLREQGVIVRHFKQERIAQFLRISIGTPEQNQALIEALGEL Sbjct: 301 RHDAAGLAAKLREQGVIVRHFKQERIAQFLRISIGTPEQNQALIEALGEL 350 Lambda K H 0.320 0.139 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 648 Number of extensions: 13 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 350 Length of database: 350 Length adjustment: 29 Effective length of query: 321 Effective length of database: 321 Effective search space: 103041 Effective search space used: 103041 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate Pf6N2E2_3251 (Histidinol-phosphate aminotransferase (EC 2.6.1.9))
to HMM TIGR01141 (hisC: histidinol-phosphate transaminase (EC 2.6.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01141.hmm # target sequence database: /tmp/gapView.5354.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01141 [M=349] Accession: TIGR01141 Description: hisC: histidinol-phosphate transaminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.5e-97 311.0 0.0 5.1e-97 310.8 0.0 1.0 1 lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3251 Histidinol-phosphate aminotransf Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3251 Histidinol-phosphate aminotransferase (EC 2.6.1.9) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 310.8 0.0 5.1e-97 5.1e-97 3 348 .. 8 349 .. 6 350 .] 0.95 Alignments for each domain: == domain 1 score: 310.8 bits; conditional E-value: 5.1e-97 TIGR01141 3 kikklepYqpgarelgekevvkLnsnEnPfgpsekvkealkeelk.klhrYpdpqalelkeala 65 +k+l pY+pg++ + + vkLn+nEnP+gps+k+++a++ el+ +l++Ypdp++ lk a+a lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3251 8 FVKNLVPYVPGEQPKLT-RLVKLNTNENPYGPSPKALAAMQTELNdNLRLYPDPNSDLLKGAVA 70 699*******9777777.6*************************99****************** PP TIGR01141 66 kylgveeenillgnGsdelielliraflepgdavlvleptysmYevsakiagaevkevplkedg 129 +y+gv+ ++++lgnGsde++ +++ +l+ + l+++ +ys Y+v+ ++g++ vpl++++ lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3251 71 RYYGVQSNQVFLGNGSDEVLAHIFHGLLQHDQPLLFPDISYSFYPVYCGLYGIQFDAVPLDAQF 134 ***************************************************************9 PP TIGR01141 130 qedleavleaakekvklvflasPnnPtGnllkreeiekvleevedalVVvDeAYieFseeasvl 193 q d ++ + k + ++f +Pn+PtG ll e +e++l++ d +VVvDeAYi+F +e +++ lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3251 135 QIDPADYA---KPNGGIIF-PNPNAPTGCLLALEAVEQILKANPDSVVVVDEAYIDFGGE-TAI 193 99988888...55555665.89********************77***************7.*** PP TIGR01141 194 ellaeypnlvvlrTlSKafgLAglRvGyaianaeiiealekvrapyn...vsslaleaavaalr 254 +l+ +ypnl+v++TlSK+ +LAglRvG+a++++e+ieale++++++n +++la + aa + lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3251 194 SLVDRYPNLLVTQTLSKSRSLAGLRVGLAVGHPELIEALERIKNSFNsypLDRLANVGGAAAFD 257 *******************************************87543339************* PP TIGR01141 255 dsdkiektveevkkererlleelkkleglevyeSkaNFvlikvke.daeelleallekgiivRd 317 d++++ +t + v+++re++ ++l++ g+ev +S aNF++++ ++ da+ l+ +l e+g+ivR+ lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3251 258 DREYFDRTCRLVIEHREWVVAQLQAK-GFEVLPSAANFIFARHPRhDAAGLAAKLREQGVIVRH 320 ************************87.9****************99****************** PP TIGR01141 318 lksaeglleeclRitvGtreenerllealke 348 +k+ + ++lRi++Gt+e+n++l+eal e lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3251 321 FKQE--RIAQFLRISIGTPEQNQALIEALGE 349 *977..48********************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (349 nodes) Target sequences: 1 (350 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 11.91 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory