Alignments for a candidate for glnA in Rhodobacter sphaeroides ATCC 17029

GapMind for Amino acid biosynthesis

Alignments for a candidate for glnA in Rhodobacter sphaeroides ATCC 17029

Align glutamine synthetase (EC 6.3.1.2) (characterized)
to candidate WP_002719482.1 RSPH17029_RS05065 glutamine synthetase

Query= BRENDA::O33342
         (457 letters)



>NCBI__GCF_000015985.1:WP_002719482.1
          Length = 459

 Score =  409 bits (1052), Expect = e-119
 Identities = 222/460 (48%), Positives = 290/460 (63%), Gaps = 10/460 (2%)

Query: 4   PGSPPLAWTELERLVAAGDVDTVIVAFTDMQGRLAGKRISGRHFVDDIATRGVECCSYLL 63
           PG+  + + EL+  V +G +DTV+VA  DMQGRL GKR   ++F+D        CC YLL
Sbjct: 2   PGN--MTFDELKEAVESGGIDTVLVACVDMQGRLMGKRFHAQYFIDG-GHHETHCCDYLL 58

Query: 64  AVDVDLNTVPGYAMASWDTGYGDMVMTPDLSTLRLIPWLPGTALVIADLVWADGSEVAVS 123
           A+D+++ TVPGY  + W+ GYGD VM PD++TLR +PW+PGTALV+ DL+    ++V  S
Sbjct: 59  AIDMEMTTVPGYKNSGWEKGYGDYVMKPDMATLRALPWMPGTALVLCDLLDHHHNDVPQS 118

Query: 124 PRSILRRQLDRLKARGLVADVATELEFIVFDQPY----RQAWASG-YRGLTPASDYNIDY 178
           PR+IL+RQ+ R +A G  A +ATELEF +F+  +        ASG  R L P S YN DY
Sbjct: 119 PRAILKRQIARAEAMGFKAMMATELEFFLFETSFDAVRNSMQASGQIRDLKPISAYNEDY 178

Query: 179 AILASSRMEPLLRDIRLGMAGAGLRFEAVKGECNMGQQEIGFRYDEALVTCDNHAIYKNG 238
            I  +S+ E ++R +R G+ GAG+  E  KGE + GQ+EI  +Y +AL T DNHAI K  
Sbjct: 179 HIFQTSKEEEVMRALRNGLYGAGIPVECSKGEADAGQEEINVKYSDALDTADNHAITKTA 238

Query: 239 AKEIADQHGKSLTFMAKYDERE-GNSCHIHVSLRGTDGSAVFADSNGPHGMSSMFRSFVA 297
            K+IA   GKS+TFMAKYD R  G+S H+H SL   DG   F D   PHGMS + R F+A
Sbjct: 239 VKDIAHAAGKSVTFMAKYDHRRAGSSSHVHQSLWTLDGKPAFFDPEAPHGMSELMRQFLA 298

Query: 298 GQLATLREFTLCYAPTINSYKRFADSSFAPTALAWGLDNRTCALRVVGHG-QNIRVECRV 356
           GQLA   E T   AP +NSYKRF    FAPT   W  DNRT   RV G G + IRVECR+
Sbjct: 299 GQLAHAEEITAFLAPYVNSYKRFCVGMFAPTKAVWSRDNRTAGFRVCGEGTKAIRVECRI 358

Query: 357 PGGDVNQYLAVAALIAGGLYGIERGLQLPEPCVGNAYQGADVERLPVTLADAAVLFEDSA 416
            G D+N YLA AAL+A GL GIE+ + L     G+ Y  A V  +P TL  AA   + S 
Sbjct: 359 GGADLNPYLACAALLAAGLDGIEKKMDLEAEMRGDMYSTAGVREIPKTLRAAAERLKGST 418

Query: 417 LVREAFGEDVVAHYLNNARVELAAFNAAVTDWERIRGFER 456
           ++REA G++VV HY + A  E++  +  VTD+E  R FER
Sbjct: 419 MLREAMGDEVVEHYHHAAEWEISEQDRVVTDFELARLFER 458


Lambda     K      H
   0.321    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 549
Number of extensions: 18
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 457
Length of database: 459
Length adjustment: 33
Effective length of query: 424
Effective length of database: 426
Effective search space:   180624
Effective search space used:   180624
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis