Alignments for a candidate for argG in Thauera aminoaromatica S2

GapMind for Amino acid biosynthesis

Alignments for a candidate for argG in Thauera aminoaromatica S2

Align Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 (characterized)
to candidate WP_004298653.1 C665_RS01910 argininosuccinate synthase

Query= SwissProt::P59846
         (400 letters)



>NCBI__GCF_000310185.1:WP_004298653.1
          Length = 409

 Score =  494 bits (1273), Expect = e-144
 Identities = 243/397 (61%), Positives = 307/397 (77%), Gaps = 7/397 (1%)

Query: 2   KIVLAYSGGLDTSIILKWLKETYRAEVIAFTADIGQGEEVEEAREKALRTGASKAIAL-- 59
           K+VLAYSGGLDTS+ILKWL++TY+ EV+ FTAD+GQGEE+E AR KAL+ G         
Sbjct: 6   KVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARAKALQFGIQPENIFID 65

Query: 60  DLKEEFVRDFVFPMMRAGAVYEGYYLLGTSIARPLIAKHLVRIAEEEGAEAIAHGATGKG 119
           DL+EEFVRDFVFPM RA  +YEG YLLGTSIARPLIAK  + IA   GA+A++HGATGKG
Sbjct: 66  DLREEFVRDFVFPMFRANTIYEGEYLLGTSIARPLIAKRQIEIARATGADAVSHGATGKG 125

Query: 120 NDQVRFELTAYALKPDIKVIAPWREWSFQGRKEMIAYAEAHGIPVPVTQEK---PYSMDA 176
           NDQVRFEL  YAL P +KVIAPWREW    R++++AYAE HGIP+ +  ++   PYSMDA
Sbjct: 126 NDQVRFELGYYALMPGVKVIAPWREWDLLSREKLLAYAEKHGIPIEMKHKQGGSPYSMDA 185

Query: 177 NLLHISYEGGVLEDPWAEPPKGMFRMTQDPEEAPDAPEYVEVEFFEGDPVAVNGERLSPA 236
           NLLHIS+EG  LE+P AE  + M+R T  PE APDA EY+++EF +GD VA++G+R+   
Sbjct: 186 NLLHISFEGRHLENPAAEAEESMWRWTVSPEAAPDAAEYIDLEFEQGDLVAIDGKRMKAH 245

Query: 237 ALLQRLNEIGGRHGVGRVDIVENRFVGMKSRGVYETPGGTILYHARRAVESLTLDREVLH 296
            LL RLNE+GG+HG+GR+D+VENR+VGMKSRG YETPGGTIL  A RA+ES+TLDREV H
Sbjct: 246 ELLARLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLRAHRAIESITLDREVAH 305

Query: 297 QRDMLSPKYAELVYYGFWYAPEREALQAYFDHVARSVTGVARLKLYKGNVYVVGRKA-PK 355
            +D L P+YA ++Y G+W++PER A+QA  DH  ++V G  RLKLYKGNV V GR +   
Sbjct: 306 LKDDLMPRYASMIYNGYWWSPERRAIQALIDHTQQTVNGWVRLKLYKGNVIVTGRDSKTD 365

Query: 356 SLYRQDLVSF-DEAGGYDQKDAEGFIKIQALRLRVRA 391
           SL+   + +F D+AG Y+QKDA GFI++ ALR+R+ A
Sbjct: 366 SLFDPTIATFEDDAGAYNQKDAHGFIRLNALRMRIAA 402


Lambda     K      H
   0.319    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 500
Number of extensions: 20
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 400
Length of database: 409
Length adjustment: 31
Effective length of query: 369
Effective length of database: 378
Effective search space:   139482
Effective search space used:   139482
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_004298653.1 C665_RS01910 (argininosuccinate synthase)
to HMM TIGR00032 (argG: argininosuccinate synthase (EC 6.3.4.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00032.hmm
# target sequence database:        /tmp/gapView.27387.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00032  [M=394]
Accession:   TIGR00032
Description: argG: argininosuccinate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.5e-143  464.7   0.0   1.8e-143  464.5   0.0    1.0  1  lcl|NCBI__GCF_000310185.1:WP_004298653.1  C665_RS01910 argininosuccinate s


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000310185.1:WP_004298653.1  C665_RS01910 argininosuccinate synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  464.5   0.0  1.8e-143  1.8e-143       1     390 [.       6     402 ..       6     406 .. 0.96

  Alignments for each domain:
  == domain 1  score: 464.5 bits;  conditional E-value: 1.8e-143
                                 TIGR00032   1 kvvlaysGGlDtsvalklleek.gaeviavtvdvGqpeedldaieekalklGae..kayviDareefvk 66 
                                               kvvlaysGGlDtsv+lk+l++   +ev+++t+d+Gq+ e+l+ ++ kal++G +  +  + D reefv+
  lcl|NCBI__GCF_000310185.1:WP_004298653.1   6 KVVLAYSGGLDTSVILKWLQDTyQCEVVTFTADLGQG-EELEPARAKALQFGIQpeNIFIDDLREEFVR 73 
                                               8*********************899***********9.9*************86314455579****** PP

                                 TIGR00032  67 dylfaaiqanavyegkYllstaLaRpliakklvevakkegaeavahGctgKGnDqvRFelsilllnpdl 135
                                               d++f+  +an++yeg+Yll+t++aRpliak+++e+a+  ga+av+hG+tgKGnDqvRFel  ++l p +
  lcl|NCBI__GCF_000310185.1:WP_004298653.1  74 DFVFPMFRANTIYEGEYLLGTSIARPLIAKRQIEIARATGADAVSHGATGKGNDQVRFELGYYALMPGV 142
                                               ********************************************************************* PP

                                 TIGR00032 136 kviaPvreleli.ReeeieyaaekGievpveke...kaysiDenllgrsiEageLEdpsteppediyel 200
                                               kviaP+re++l  Re++++ya+++Gi+++++++   ++ys+D nll++s E+ +LE+p +e  e ++++
  lcl|NCBI__GCF_000310185.1:WP_004298653.1 143 KVIAPWREWDLLsREKLLAYAEKHGIPIEMKHKqggSPYSMDANLLHISFEGRHLENPAAEAEESMWRW 211
                                               **********987****************997634479******************************* PP

                                 TIGR00032 201 vkdpiektedepevveieFekGvPvalngeslepvelilkaneiagkhGvGriDivEdRiiglKsReiY 269
                                               +++p++++ d +e++++eFe+G  va++g+++++ el+ ++ne++gkhG+Gr+D vE+R++g+KsR++Y
  lcl|NCBI__GCF_000310185.1:WP_004298653.1 212 TVSPEAAP-DAAEYIDLEFEQGDLVAIDGKRMKAHELLARLNELGGKHGIGRLDLVENRYVGMKSRGCY 279
                                               **888887.************************************************************ PP

                                 TIGR00032 270 EapalllLikAhkdLetlvltkdvlefkeiveekyseliYkGlwfdplaealdalikktqervtGtvrv 338
                                               E+p+ ++L++Ah+++e+ +l+++v+++k+    +y+ +iY+G+w++p++ a++ali++tq+ v+G+vr+
  lcl|NCBI__GCF_000310185.1:WP_004298653.1 280 ETPGGTILLRAHRAIESITLDREVAHLKDDLMPRYASMIYNGYWWSPERRAIQALIDHTQQTVNGWVRL 348
                                               ********************************************************************* PP

                                 TIGR00032 339 klfkGnaivigrk.seyslYdeelvsfek.dkefdqkdaiGfikirglqikvyr 390
                                               kl+kGn+iv gr+ +++sl+d+++++fe    +++qkda Gfi++++l++++ +
  lcl|NCBI__GCF_000310185.1:WP_004298653.1 349 KLYKGNVIVTGRDsKTDSLFDPTIATFEDdAGAYNQKDAHGFIRLNALRMRIAA 402
                                               *************8999***********9445799*************998766 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (394 nodes)
Target sequences:                          1  (409 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.01
# Mc/sec: 12.13
//
[ok]

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis