Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate WP_004514199.1 GMET_RS00375 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit A
Query= SwissProt::O06491 (485 letters) >NCBI__GCF_000012925.1:WP_004514199.1 Length = 485 Score = 563 bits (1451), Expect = e-165 Identities = 279/483 (57%), Positives = 363/483 (75%), Gaps = 2/483 (0%) Query: 1 MSLFDHKITELKQLIHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDE 60 M LFD I EL + +KE+ + RI+AVD KV A++ + E A A A+ D Sbjct: 1 MELFDLTIHELHDRLKRKELSSVEATRAMLARIEAVDPKVNAYITVTPEEALAAAEAADR 60 Query: 61 AVDGRSEHGLLFGMPIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIG 120 + + L G+P+ +KD VTKG+RTTC+SKIL+NF P YD T V +L++A AV +G Sbjct: 61 RI-AAGDMAPLTGIPVALKDIFVTKGIRTTCASKILDNFVPPYDGTAVAKLKEAGAVIVG 119 Query: 121 KLNMDEFAMGSSTENSAYKLTKNPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIR 180 KLN DEFAMGSS E+SA+ T+NPWNL+ +PGGSS GSAAA+AA +LG+DTGGSIR Sbjct: 120 KLNQDEFAMGSSGESSAFGPTRNPWNLECIPGGSSSGSAAAIAARTATATLGTDTGGSIR 179 Query: 181 QPASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTS 240 QPAS CG VGL+PTYGRVSRYG++A+ASSLDQ+GP+TR V D A +LQA++G D MDSTS Sbjct: 180 QPASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLQAVAGHDPMDSTS 239 Query: 241 ANVDVPDFLSSLTGDIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLP 300 V VPD+ LTGD+KGLK+ +PKEY EG+ + ++++ A++ GLGA + ++SLP Sbjct: 240 VEVPVPDYAKGLTGDVKGLKLGLPKEYYIEGLDPDVKKALDEAIETYRGLGAEFVDISLP 299 Query: 301 HSKYALATYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIM 360 H+ YA+ATYYL++++EAS+NLAR++G+R+G+RT+ A NLID++++TR+EGFG+EVKRRIM Sbjct: 300 HTDYAVATYYLIATAEASSNLARYEGVRFGHRTEGAANLIDMFRKTRSEGFGDEVKRRIM 359 Query: 361 LGTFALSSGYYDAYYKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLT 420 +GT+ALSSGYYDAYY KAQKVRTLI +DF FE D I+ P PTPAFKIGE T DPL Sbjct: 360 IGTYALSSGYYDAYYLKAQKVRTLIMQDFLKAFEAVDAILTPVAPTPAFKIGEKTSDPLR 419 Query: 421 MYANDILTIPVNLAGVPGISVPCGLAD-GLPLGLQIIGKHFDESTVYRVAHAFEQATDHH 479 MY +DI TIPVNLAG +SVP G++ GLP+GLQ+IG+ F E T+ R AHAFEQAT H Sbjct: 420 MYLSDIFTIPVNLAGTCAVSVPAGMSGAGLPIGLQLIGRPFGEETILRAAHAFEQATAWH 479 Query: 480 KAK 482 K Sbjct: 480 TQK 482 Lambda K H 0.315 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 620 Number of extensions: 35 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 485 Length of database: 485 Length adjustment: 34 Effective length of query: 451 Effective length of database: 451 Effective search space: 203401 Effective search space used: 203401 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
Align candidate WP_004514199.1 GMET_RS00375 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit A)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00132.hmm # target sequence database: /tmp/gapView.8119.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00132 [M=466] Accession: TIGR00132 Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.5e-197 641.7 0.0 4e-197 641.5 0.0 1.0 1 lcl|NCBI__GCF_000012925.1:WP_004514199.1 GMET_RS00375 Asp-tRNA(Asn)/Glu-t Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000012925.1:WP_004514199.1 GMET_RS00375 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit A # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 641.5 0.0 4e-197 4e-197 2 464 .. 12 476 .. 11 478 .. 0.99 Alignments for each domain: == domain 1 score: 641.5 bits; conditional E-value: 4e-197 TIGR00132 2 kellkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva.ke.kklagipiavKdn 68 ++ lk+ke+s++e+++++l+rieav+ k+na+++vt+e+al++a++ d+++a + +l+gip+a+Kd lcl|NCBI__GCF_000012925.1:WP_004514199.1 12 HDRLKRKELSSVEATRAMLARIEAVDPKVNAYITVTPEEALAAAEAADRRIAaGDmAPLTGIPVALKDI 80 67899**********************************************975546************ PP TIGR00132 69 iavkdiettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknPeneerv 137 +++k+i+ttcaSkiL+n+v+pyd t v +lkeaga+i+Gk N DEFamGss+e+Safg+t+nP+n e + lcl|NCBI__GCF_000012925.1:WP_004514199.1 81 FVTKGIRTTCASKILDNFVPPYDGTAVAKLKEAGAVIVGKLNQDEFAMGSSGESSAFGPTRNPWNLECI 149 ********************************************************************* PP TIGR00132 138 pGGSsgGsaaavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGilakk 206 pGGSs+Gsaaa+aa ++ +lg+DTGgSiRqPAs+cg+vGl+PtYG+vSRyG++ayasSldq+G+++++ lcl|NCBI__GCF_000012925.1:WP_004514199.1 150 PGGSSSGSAAAIAARTATATLGTDTGGSIRQPASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRD 218 ********************************************************************* PP TIGR00132 207 vedialvldvisgkDkkDstslevkveelleelkkdlkglkvgvvkelseesldkevkekfekllekle 275 v+d al+l++++g+D +Dsts+ev+v+++ + l+ d+kglk+g+ ke+ e+ld +vk+++++++e+ + lcl|NCBI__GCF_000012925.1:WP_004514199.1 219 VTDCALMLQAVAGHDPMDSTSVEVPVPDYAKGLTGDVKGLKLGLPKEYYIEGLDPDVKKALDEAIETYR 287 ********************************************************************* PP TIGR00132 276 elgaeivevslpsvklalaiYyiispsEassnlarydgiryGkrveelkslkelyaktRsegfgeevkr 344 lgae+v++slp+ ++a+a+Yy+i+++Eassnlary+g+r+G+r+e + +l +++ ktRsegfg+evkr lcl|NCBI__GCF_000012925.1:WP_004514199.1 288 GLGAEFVDISLPHTDYAVATYYLIATAEASSNLARYEGVRFGHRTEGAANLIDMFRKTRSEGFGDEVKR 356 ********************************************************************* PP TIGR00132 345 RimlGayalskeyydkyykkAqkvrtliidefeklfeevDvivsptaptlafklgekaedplemylsDv 413 Rim+G+yals++yyd+yy+kAqkvrtli+++f k+fe vD i++p+apt afk+gek++dpl+mylsD+ lcl|NCBI__GCF_000012925.1:WP_004514199.1 357 RIMIGTYALSSGYYDAYYLKAQKVRTLIMQDFLKAFEAVDAILTPVAPTPAFKIGEKTSDPLRMYLSDI 425 ********************************************************************* PP TIGR00132 414 ltvpanlaGlpaisvPlgkkekglpiGlqiigkafddkkllsvakaleqal 464 +t+p+nlaG++a+svP+g++ glpiGlq+ig+ f ++++l++a+a+eqa+ lcl|NCBI__GCF_000012925.1:WP_004514199.1 426 FTIPVNLAGTCAVSVPAGMSGAGLPIGLQLIGRPFGEETILRAAHAFEQAT 476 ************************************************986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (466 nodes) Target sequences: 1 (485 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 7.45 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory